ES2185493B1 - Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala. - Google Patents
Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala.Info
- Publication number
- ES2185493B1 ES2185493B1 ES200101545A ES200101545A ES2185493B1 ES 2185493 B1 ES2185493 B1 ES 2185493B1 ES 200101545 A ES200101545 A ES 200101545A ES 200101545 A ES200101545 A ES 200101545A ES 2185493 B1 ES2185493 B1 ES 2185493B1
- Authority
- ES
- Spain
- Prior art keywords
- vitro
- angiogenesis
- biological activity
- pathological conditions
- evaluating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 6
- 150000001875 compounds Chemical class 0.000 title abstract 3
- 238000000338 in vitro Methods 0.000 title abstract 3
- 230000004071 biological effect Effects 0.000 title abstract 2
- 230000001575 pathological effect Effects 0.000 title abstract 2
- 230000033115 angiogenesis Effects 0.000 abstract 3
- 238000002474 experimental method Methods 0.000 abstract 2
- 238000012216 screening Methods 0.000 abstract 2
- 206010027476 Metastases Diseases 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 230000002491 angiogenic effect Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 210000004204 blood vessel Anatomy 0.000 abstract 1
- 238000011161 development Methods 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000009401 metastasis Effects 0.000 abstract 1
- 230000000877 morphologic effect Effects 0.000 abstract 1
- 238000012545 processing Methods 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
- 238000011160 research Methods 0.000 abstract 1
- 238000010937 topological data analysis Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Método para evaluar in vitro en condiciones fisiológicas o patológicas relevantes la actividad biológica de compuestos a gran escala. La formación de vasos sanguíneos (angiogénesis) es un proceso necesario para el crecimiento y diseminación (metástasis) de los tumores. El desarrollo de sistemas de examen "screening" para la identificación de compuestos inhibidores de este proceso es un campo de investigación exhaustiva, que en los últimos años camina hacia el uso de sistemas informáticos y robotizados. Se presenta un sistema de adquisición y procesamiento de imágenes, diseñado y desarrollado para ser el núcleo de una plataforma dirigida a escenarios de "screening" masivo en experimentos basados en modelos de angiogénesis "in vitro"'' miniaturizados. El sistema adquiere y procesa secuencialmente imágenes obtenidas mediante microscopio y cámara digital de los pocillos de microplacas estándar. Se propone un procedimiento de cuantificación del grado de angiogénesis basado en un análisis morfológico y topológico de la red de vasos generados, que proporciona un valor añadido al tradicional parámetro de densidad microvascular. En su cálculo se toman en consideración factores adicionales como conectividad y longitud de los vasos. El sistema cumple con los requerimientos básicos en estos experimentos: objetividad, reproducibilidad y rapidez; alcanzados por la capacidad automática de cuantificación de la actividad angiogénica desarrollada.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200101545A ES2185493B1 (es) | 2001-07-03 | 2001-07-03 | Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala. |
| PCT/ES2002/000327 WO2003005301A1 (es) | 2001-07-03 | 2002-07-01 | Método para evaluar in vitro en condiciones fisiológicas o patológicas relevantes la actividad biológica de compuestos a gran escala |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200101545A ES2185493B1 (es) | 2001-07-03 | 2001-07-03 | Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2185493A1 ES2185493A1 (es) | 2003-04-16 |
| ES2185493B1 true ES2185493B1 (es) | 2004-06-16 |
Family
ID=8498272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200101545A Expired - Fee Related ES2185493B1 (es) | 2001-07-03 | 2001-07-03 | Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala. |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES2185493B1 (es) |
| WO (1) | WO2003005301A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8588492B2 (en) | 2007-11-20 | 2013-11-19 | Koninklijke Philips N.V. | Visualization of vascularization |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPM425294A0 (en) * | 1994-03-04 | 1994-03-31 | Australian National University, The | In-vitro angiogenesis assay |
| GB9720987D0 (en) * | 1997-10-01 | 1997-12-03 | Biocure Ltd | A multicellular in vitro assay of angiogenesis |
| AU768732B2 (en) * | 1999-05-24 | 2004-01-08 | Cellomics, Inc. | Method and system for general purpose analysis of experimental data |
| US6716588B2 (en) * | 1999-12-09 | 2004-04-06 | Cellomics, Inc. | System for cell-based screening |
| CA2418077A1 (en) * | 2000-09-06 | 2002-03-14 | Universite Laval | In vitro human angiogenesis model |
-
2001
- 2001-07-03 ES ES200101545A patent/ES2185493B1/es not_active Expired - Fee Related
-
2002
- 2002-07-01 WO PCT/ES2002/000327 patent/WO2003005301A1/es not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003005301A1 (es) | 2003-01-16 |
| ES2185493A1 (es) | 2003-04-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20030416 Kind code of ref document: A1 |
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| FG2A | Definitive protection |
Ref document number: 2185493B1 Country of ref document: ES |
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| FD2A | Announcement of lapse in spain |
Effective date: 20180807 |