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ES2182703B1 - Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa, 4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el pr cedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal. - Google Patents

Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa, 4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el pr cedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal.

Info

Publication number
ES2182703B1
ES2182703B1 ES200101419A ES200101419A ES2182703B1 ES 2182703 B1 ES2182703 B1 ES 2182703B1 ES 200101419 A ES200101419 A ES 200101419A ES 200101419 A ES200101419 A ES 200101419A ES 2182703 B1 ES2182703 B1 ES 2182703B1
Authority
ES
Spain
Prior art keywords
beta
bdg
substrate
galactopiranosil
xylosa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200101419A
Other languages
English (en)
Other versions
ES2182703A1 (es
Inventor
Francisco J. Cañada Vicinay
Guillermo Corrales Morales
Alfonso Fernandez-Mayoralas
Manuel Martin Lomas
Juan Jose Aragon Reyes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Universidad Autonoma de Madrid
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Universidad Autonoma de Madrid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES200101419A priority Critical patent/ES2182703B1/es
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Universidad Autonoma de Madrid filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to MXPA03011869A priority patent/MXPA03011869A/es
Priority to EP02740774A priority patent/EP1408118B1/en
Priority to AT02740774T priority patent/ATE321876T1/de
Priority to RU2004101406/13A priority patent/RU2316593C2/ru
Priority to ES02740774T priority patent/ES2260444T3/es
Priority to PT02740774T priority patent/PT1408118E/pt
Priority to CA2451065A priority patent/CA2451065C/en
Priority to PCT/ES2002/000297 priority patent/WO2002103038A1/es
Priority to AU2002314215A priority patent/AU2002314215B2/en
Priority to JP2003505359A priority patent/JP4115933B2/ja
Priority to DE60210278T priority patent/DE60210278T2/de
Publication of ES2182703A1 publication Critical patent/ES2182703A1/es
Priority to US10/738,378 priority patent/US7537909B2/en
Application granted granted Critical
Publication of ES2182703B1 publication Critical patent/ES2182703B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Extraction Or Liquid Replacement (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Un procedimiento enzimático para obtener 4-O-{be}-D- galactopiranosil-D-xilosa, 4-O-{be}-D-galactopiranosil-D-xilosa obtenida de acuerdo con el procedimiento, composiciones que la contienen y su uso en la evaluación de la lactasa intestinal. Un procedimiento enzimático para obtener 4-O-{be}-D- galactopiranosil-D-xilosa útil, como tal o en composiciones o disoluciones, en la evaluación in vivo de la actividad lactasa intestinal en humanos, que comprende las etapas de preparar una mezcla de reacción de D-xilosa, un {be}-D-galactopiranósido, y un medio de reacción que comprende agua tamponada a un pH entre 5.0 y 9.0; añadiéndose 10 a 1.000 unidades de {be}-D-galactosidasa por gramo de {be}-D-galactopiranósido; someter la mezcla de reacción a una reacción a una temperatura entre una temperatura superior al punto de congelación de la mezcla de reacción y 45ºC, durante 2 a 48 horas; parar la reacción mediante desactivación de la {be}-D-galactosidasa; y aislar y cristalizar, las fracciones que contienen 4-O-{be}-D-galactopiranosil-D-xilosa en una mezcla de cristalización seleccionada entre mezclas de acetona/metanol en una relación entre 5/1 a 20/1, y mezclas de acetona/agua en una relación entre 5/1 a 20/1.
ES200101419A 2001-06-18 2001-06-18 Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa, 4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el pr cedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal. Expired - Fee Related ES2182703B1 (es)

Priority Applications (13)

Application Number Priority Date Filing Date Title
ES200101419A ES2182703B1 (es) 2001-06-18 2001-06-18 Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa, 4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el pr cedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal.
JP2003505359A JP4115933B2 (ja) 2001-06-18 2002-06-14 ４−Ｏ−β−Ｄ−ガラクトピラノシル−Ｄ−キシロースの酵素的製造方法、該方法によって得られる４−Ｏ−β−Ｄ−ガラクトピラノシル−Ｄ−キシロース、該キシロースを含有する組成物および腸ラクターゼの評価における該キシロースの使用
AT02740774T ATE321876T1 (de) 2001-06-18 2002-06-14 Enzymatisches verfahren zur herstellung von 4-0-b-d-galactopyranosyl-d-xylose, mit diesem verfahren gewonnene 4-0-b-d-galactopyranosyl-d-xylose, diese enthaltende zusammensetzungen und verwendung davon beim quantifiizieren intestinaler lactase
RU2004101406/13A RU2316593C2 (ru) 2001-06-18 2002-06-14 Ферментативный способ получения 4-о-бета-d-галактопиранозил-d-ксилозы
ES02740774T ES2260444T3 (es) 2001-06-18 2002-06-14 Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa,4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el procedimiento, composiciones que la continenen y su uso en la evaluacion de la lactasa intestinal.
PT02740774T PT1408118E (pt) 2001-06-18 2002-06-14 Metodo enzimatico de producao de 4-0-b-d-galactopiranosil-d-xilose, 4-0-b-d-xilose obtida com o referido metodo, composicoes que as contem e a sua utilizacao na avaliacao da lactase intestinal
MXPA03011869A MXPA03011869A (es) 2001-06-18 2002-06-14 Un procedimiento enzimatico para obtener 4-o-??-d-galactopiranosil-d-xilosa, 4-o-??-d-galactopiranosil-d-xilosa obtenida de acuerdo con el procedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal.
PCT/ES2002/000297 WO2002103038A1 (es) 2001-06-18 2002-06-14 Un procedimiento enzimático para obtener 4-0-b-d galactopiranosil-d-xilosa obtenida deacuerdo con el procedimiento, composiciones que la contienen y su uso en la evaluación de la lactasa intestinal
AU2002314215A AU2002314215B2 (en) 2001-06-18 2002-06-14 Enzymatic method of producing 4-0-beta-D galactopyranosyl-D-xylose, 4-0-beta-D-galactopyranosyl-D-xylose obtained using said method, compositions containing same and the use thereof in evaluating intestinal lactase
EP02740774A EP1408118B1 (en) 2001-06-18 2002-06-14 Enzymatic method of producing 4-0-b-d galactopyranosyl-d-xylose, 4-0-b-d-galactopyranosyl-d-xylose obtained using said method, compositions containing same and the use thereof in evaluating intestinal lactase
DE60210278T DE60210278T2 (de) 2001-06-18 2002-06-14 ENZYMATISCHES VERFAHREN ZUR HERSTELLUNG VON 4-O-ß-D-GALACTOPYRANOSYL-D-XYLOSE, MIT DIESEM VERFAHREN GEWONNENE 4-O-ß-D-GALACTOPYRANOSYL-D-XYLOSE, DIESE ENTHALTENDE ZUSAMMENSETZUNGEN UND VERWENDUNG DAVON BEIM QUANTIFIIZIEREN INTESTINALER LACTASE
CA2451065A CA2451065C (en) 2001-06-18 2002-06-14 Enzymatic method of producing 4-0-beta-d-galactopyranosyl-d-xylose
US10/738,378 US7537909B2 (en) 2001-06-18 2003-12-17 Enzymatic method of producing 4-O-β-D-galactopyranosyl-D-xylose, 4-O-β-D-galactopyranosyl-D-xylose obtained using said method, compositions contain same and the use thereof in evaluating intestinal lactase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200101419A ES2182703B1 (es) 2001-06-18 2001-06-18 Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa, 4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el pr cedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal.

Publications (2)

Publication Number Publication Date
ES2182703A1 ES2182703A1 (es) 2003-03-01
ES2182703B1 true ES2182703B1 (es) 2004-06-01

Family

ID=8498115

Family Applications (2)

Application Number Title Priority Date Filing Date
ES200101419A Expired - Fee Related ES2182703B1 (es) 2001-06-18 2001-06-18 Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa, 4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el pr cedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal.
ES02740774T Expired - Lifetime ES2260444T3 (es) 2001-06-18 2002-06-14 Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa,4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el procedimiento, composiciones que la continenen y su uso en la evaluacion de la lactasa intestinal.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES02740774T Expired - Lifetime ES2260444T3 (es) 2001-06-18 2002-06-14 Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa,4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el procedimiento, composiciones que la continenen y su uso en la evaluacion de la lactasa intestinal.

Country Status (12)

Country Link
US (1) US7537909B2 (es)
EP (1) EP1408118B1 (es)
JP (1) JP4115933B2 (es)
AT (1) ATE321876T1 (es)
AU (1) AU2002314215B2 (es)
CA (1) CA2451065C (es)
DE (1) DE60210278T2 (es)
ES (2) ES2182703B1 (es)
MX (1) MXPA03011869A (es)
PT (1) PT1408118E (es)
RU (1) RU2316593C2 (es)
WO (1) WO2002103038A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9128100B2 (en) 2011-01-14 2015-09-08 Venter Pharma, S.L. Non-invasive diagnostic method for the evaluation of intestinal lactase deficiency (hypolactasia)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1887017A1 (en) * 2006-08-09 2008-02-13 Friesland Brands B.V. Prebiotic carbohydrate
CN107050915A (zh) * 2017-06-01 2017-08-18 湖北恒贸茶油有限公司 带结晶导出区的茶油结晶罐

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2023556A6 (es) * 1990-06-18 1992-01-16 Consejo Superior Investigacion Procedimiento de obtencion de 4-o-beta-d-galactopiranosil-d-xilosa utilizable para la evaluacion diagnostica de la lactasa intestinal.
ES2100131A1 (es) * 1995-11-08 1997-06-01 Consejo Superior Investigacion Procedimiento enzimatico de obtencion de beta-d-galactopiranosil-d-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal.
ES2130073A1 (es) * 1997-05-28 1999-06-16 Consejo Superior Investigacion Mejoras en el procedimiento de obtencion de beta-d-galactopiranosil-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228274A (en) * 1976-09-28 1980-10-14 Merck & Co., Inc. 1-Substituted glycopyranosides
US4130709A (en) * 1977-12-08 1978-12-19 Eli Lilly And Company Pleuromutilin glycoside derivatives
SE8203925D0 (sv) * 1982-06-23 1982-06-23 Svenska Sockerfabriks Ab New and novel glycosides, glycoconjugates and processes for their preparation
WO1995008645A1 (en) * 1993-09-23 1995-03-30 New England Biolabs, Inc. Isolation and composition of novel glycosidases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2023556A6 (es) * 1990-06-18 1992-01-16 Consejo Superior Investigacion Procedimiento de obtencion de 4-o-beta-d-galactopiranosil-d-xilosa utilizable para la evaluacion diagnostica de la lactasa intestinal.
ES2100131A1 (es) * 1995-11-08 1997-06-01 Consejo Superior Investigacion Procedimiento enzimatico de obtencion de beta-d-galactopiranosil-d-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal.
ES2130073A1 (es) * 1997-05-28 1999-06-16 Consejo Superior Investigacion Mejoras en el procedimiento de obtencion de beta-d-galactopiranosil-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal.

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ARAGÓN, J.J. et al., "Evaluation of rat intestinal lactse in vivo with 4-galactosylxylose", CLINICA CHIMICA ACTA, Vol. 210, 30 septiembre 1992, Amsterdam, NL, páginas 221-226. Todo el documento. *
LÓPEZ, R. et al., "Enzymic beta-galactosidation of beta-xylopyranosides", BIOTECHNOL. LETT., Vol. 13, nº10, 1991, páginas 705-710. Todo el documento. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9128100B2 (en) 2011-01-14 2015-09-08 Venter Pharma, S.L. Non-invasive diagnostic method for the evaluation of intestinal lactase deficiency (hypolactasia)
US9529000B2 (en) 2011-01-14 2016-12-27 Venter Pharma, S.L. Non-invasive diagnostic method for the evaluation of intestinal lactase deficiency (hypolactasia)

Also Published As

Publication number Publication date
JP2004537298A (ja) 2004-12-16
ATE321876T1 (de) 2006-04-15
WO2002103038A1 (es) 2002-12-27
RU2316593C2 (ru) 2008-02-10
DE60210278T2 (de) 2006-12-14
US20040241811A1 (en) 2004-12-02
RU2004101406A (ru) 2005-05-10
CA2451065A1 (en) 2002-12-27
JP4115933B2 (ja) 2008-07-09
PT1408118E (pt) 2006-08-31
MXPA03011869A (es) 2005-03-07
CA2451065C (en) 2012-09-11
AU2002314215B2 (en) 2006-11-16
EP1408118A1 (en) 2004-04-14
EP1408118B1 (en) 2006-03-29
DE60210278D1 (de) 2006-05-18
ES2260444T3 (es) 2006-11-01
US7537909B2 (en) 2009-05-26
ES2182703A1 (es) 2003-03-01

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