ES2179821T3 - Celulas pancreaticas secretoras de hormonas y mantenimiento en cultivo a largo plazo. - Google Patents
Celulas pancreaticas secretoras de hormonas y mantenimiento en cultivo a largo plazo.Info
- Publication number
- ES2179821T3 ES2179821T3 ES92914162T ES92914162T ES2179821T3 ES 2179821 T3 ES2179821 T3 ES 2179821T3 ES 92914162 T ES92914162 T ES 92914162T ES 92914162 T ES92914162 T ES 92914162T ES 2179821 T3 ES2179821 T3 ES 2179821T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- hormone
- secretion
- insulin
- hormones
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229940088597 hormone Drugs 0.000 title abstract 6
- 239000005556 hormone Substances 0.000 title abstract 6
- 230000007774 longterm Effects 0.000 title abstract 2
- 238000012423 maintenance Methods 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 10
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 6
- 102000004877 Insulin Human genes 0.000 abstract 3
- 108090001061 Insulin Proteins 0.000 abstract 3
- 229940079593 drug Drugs 0.000 abstract 3
- 239000003814 drug Substances 0.000 abstract 3
- 229940125396 insulin Drugs 0.000 abstract 3
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 abstract 2
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 abstract 2
- 238000004113 cell culture Methods 0.000 abstract 2
- 229940011871 estrogen Drugs 0.000 abstract 2
- 239000000262 estrogen Substances 0.000 abstract 2
- 229940028334 follicle stimulating hormone Drugs 0.000 abstract 2
- 230000003248 secreting effect Effects 0.000 abstract 2
- 230000028327 secretion Effects 0.000 abstract 2
- 102000051325 Glucagon Human genes 0.000 abstract 1
- 108060003199 Glucagon Proteins 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 102000009151 Luteinizing Hormone Human genes 0.000 abstract 1
- 108010073521 Luteinizing Hormone Proteins 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 238000004166 bioassay Methods 0.000 abstract 1
- 229960004407 chorionic gonadotrophin Drugs 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 206010012601 diabetes mellitus Diseases 0.000 abstract 1
- 210000004696 endometrium Anatomy 0.000 abstract 1
- 230000003325 follicular Effects 0.000 abstract 1
- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 abstract 1
- 229960004666 glucagon Drugs 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000003914 insulin secretion Effects 0.000 abstract 1
- 229940040129 luteinizing hormone Drugs 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000002611 ovarian Effects 0.000 abstract 1
- 210000001672 ovary Anatomy 0.000 abstract 1
- 210000000496 pancreas Anatomy 0.000 abstract 1
- 230000001817 pituitary effect Effects 0.000 abstract 1
- 239000000583 progesterone congener Substances 0.000 abstract 1
- 230000006259 progesterone secretion Effects 0.000 abstract 1
- 238000009256 replacement therapy Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000010257 thawing Methods 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 210000001685 thyroid gland Anatomy 0.000 abstract 1
- 231100000041 toxicology testing Toxicity 0.000 abstract 1
- 210000002993 trophoblast Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0613—Cells from endocrine organs
- C12N5/0617—Thyroid and parathyroid glands
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
- C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/31—Pituitary sex hormones, e.g. follicle-stimulating hormone [FSH], luteinising hormone [LH]; Chorionic gonadotropins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Reproductive Health (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Endocrinology (AREA)
- Gynecology & Obstetrics (AREA)
- Developmental Biology & Embryology (AREA)
- Hematology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pregnancy & Childbirth (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Data Exchanges In Wide-Area Networks (AREA)
Abstract
SE PREVEN METODOS PARA EL ESTABLECIMIENTO Y MANTENIMIENTO EN CULTIVOS A LARGO PLAZO DE CELULAS SECRETORAS DE HORMONAS. LAS CELULAS SON DERIVADAS DE TEJIDOS HUMANOS O ANIMALES TUMOROSOS O NO, QUE INCLUYEN OVARIOS, ENDOMETRIO, TROFOBLASTOS, PITUITARIA, TIROIDES Y PANCREAS. LAS CELULAS SECRETAN EN EL MEDIO CULTIVO HORMONAS TALES COMO EXTROGENOS, PROGESTINAS, HORMONA ESTIMULANTE FOLICULAR, TIROXINA, GLUCAGON, E INSULINA, DEPENDIENDO DEL ORIGEN DEL TEJIDO DE LOS CULTIVOS DE CELULAS INDIVIDUALES. EL CONTACTO CON UN SECRETOR APROPIADO PROVOCA QUE LAS CELULAS RESPONDAN CON UNA SECRECION DE HORMONAS MAYOR. POR EJEMPLO, CELULAS DE FOLICULO DE OVARIO RESPONDEN A LA HORMONA ESTIMULANTE DE FOLICULO CON UNA SECRECION AUMENTADA DE EXTROGENO Y PROGESTERONA. LAS CELULAS PANCREATICAS RESPONDEN A LA GLUCOSA ELEVADA CON UNA SECRECION AUMENTADA DE INSULINA. LAS CELULAS PROLIFERAN "IN VITRO" POR UN AÑO O MAS, Y DURANTE ESTE TIEMPO RETIENEN SU PERFIL DE SECRECION HORMONAL. LAS CELULAS SE PUEDEN CONGELAR PARA ALMACENARLAS, Y RETIENEN SU PERFIL DE SECRECION HORMONAL DESPUES DE LA DESCONGELACION. LOS CULTIVOS CELULARES SON UTILES PARA LA PRODUCCION DE HORMONAS HUMANAS, PARA EL BIO ANALISIS DE MEDICAMENTOS TALES COMO LAS GONADOTROPINAS TERAPEUTICAS, PARA DETECTAR LA EFICACIA DE UN MEDICAMENTO Y SU DISEÑO, Y PARA LA DETECCION DE LA TOXICIDAD DE MEDICAMENTOS Y PRODUCTOS QUIMICOS. LAS CELULAS TAMBIEN SE PUEDEN IMPLANTAR EN UN INDIVIDUO PARA SUBSANAR UNA SECRECION HORMONAL DEFICIENTE. POR EJEMPLO, SE PUEDEN IMPLANTAR CELULAS PANCREATICAS SECRETORAS DE INSULINA EN UN INDIVIDUO DIABETICO COMO UNA TERAPIA DE SUBSTITUCION O DE REFUERZO A LA INSULINA ADMINISTRADA EXTERNAMENTE.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71997791A | 1991-06-24 | 1991-06-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2179821T3 true ES2179821T3 (es) | 2003-02-01 |
Family
ID=24892163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES92914162T Expired - Lifetime ES2179821T3 (es) | 1991-06-24 | 1992-06-23 | Celulas pancreaticas secretoras de hormonas y mantenimiento en cultivo a largo plazo. |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US5928942A (es) |
| EP (1) | EP0605428B9 (es) |
| JP (1) | JPH06508527A (es) |
| AT (1) | ATE215605T1 (es) |
| AU (2) | AU2228592A (es) |
| CA (1) | CA2112112A1 (es) |
| DE (2) | DE69232535D1 (es) |
| DK (1) | DK0605428T5 (es) |
| ES (1) | ES2179821T3 (es) |
| WO (1) | WO1993000441A1 (es) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6352707B1 (en) | 1992-02-24 | 2002-03-05 | Anton-Lewis Usala | Transplant encapsulation in a hydrogel matrix to obscure immune recognition |
| US6231881B1 (en) | 1992-02-24 | 2001-05-15 | Anton-Lewis Usala | Medium and matrix for long-term proliferation of cells |
| JP2813467B2 (ja) * | 1993-04-08 | 1998-10-22 | ヒューマン・セル・カルチャーズ・インコーポレーテッド | 細胞培養法および培地 |
| US6008047A (en) * | 1993-04-08 | 1999-12-28 | Livercell L.L.C. | Cell culturing method and medium |
| US5534404A (en) * | 1993-12-10 | 1996-07-09 | Cytotherapeutics, Inc. | Glucose responsive insulin secreting β-cell lines and method for producing same |
| US5834308A (en) * | 1994-04-28 | 1998-11-10 | University Of Florida Research Foundation, Inc. | In vitro growth of functional islets of Langerhans |
| US6703017B1 (en) | 1994-04-28 | 2004-03-09 | Ixion Biotechnology, Inc. | Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures |
| US6001647A (en) * | 1994-04-28 | 1999-12-14 | Ixion Biotechnology, Inc. | In vitro growth of functional islets of Langerhans and in vivo uses thereof |
| US6485723B1 (en) | 1995-02-10 | 2002-11-26 | Purdue Research Foundation | Enhanced submucosal tissue graft constructs |
| US5695998A (en) * | 1995-02-10 | 1997-12-09 | Purdue Research Foundation | Submucosa as a growth substrate for islet cells |
| JPH11514877A (ja) * | 1995-10-25 | 1999-12-21 | ユニバーシティ オブ フロリダ リサーチ ファウンデイション，インコーポレイテッド | 機能性ランゲルハンス島のインビトロ成長およびそのインビボの使用 |
| US6043092A (en) * | 1996-03-18 | 2000-03-28 | University Of Pittsburgh | Cell culture media for mammalian cells |
| FR2757371B1 (fr) * | 1996-12-20 | 1999-03-26 | Mxm | Dispositif elastique a grande capacite d'allongement pour l'extension de tissu vivant |
| AU4899397A (en) | 1997-02-06 | 1998-08-26 | Genetics Institute, Llc | Human sdf-5 protein and compositions |
| US6165748A (en) | 1997-07-11 | 2000-12-26 | Genetics Institute, Inc. | Frazzled nucleotide sequences and expression products |
| US6074874A (en) * | 1997-08-29 | 2000-06-13 | University Of Pittsburgh | Epithelial cell cultures for in vitro testing |
| US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| AU778929B2 (en) * | 1999-12-06 | 2004-12-23 | General Hospital Corporation, The | Pancreatic stem cells and their use in transplantation |
| US20050042749A1 (en) | 2001-05-16 | 2005-02-24 | Carpenter Melissa K. | Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease |
| CN100580079C (zh) | 2000-05-17 | 2010-01-13 | 杰龙公司 | 神经祖细胞群体 |
| CA2415108A1 (en) * | 2000-06-30 | 2002-01-10 | Amcyte, Inc. | Culturing pancreatic stem cells having a specified, intermediate stage of development |
| US6759039B2 (en) * | 2000-06-30 | 2004-07-06 | Amcyte, Inc. | Culturing pancreatic stem cells having a specified, intermediate stage of development |
| US6468989B1 (en) * | 2000-07-13 | 2002-10-22 | Dow Pharmaceutical Sciences | Gel compositions containing metronidazole |
| WO2002079457A1 (en) | 2001-03-29 | 2002-10-10 | Ixion Biotechnology, Inc. | Method for transdifferentiation of non-pancreatic stem cells to the pancreatic differentiation pathway |
| US6642003B2 (en) | 2001-08-02 | 2003-11-04 | Cedars-Sinai Medical Center | Human glucose-dependent insulin-secreting cell line |
| GB2415432B (en) * | 2001-12-07 | 2006-09-06 | Geron Corp | Islet cells from human embryonic stem cells |
| US7101546B2 (en) * | 2001-12-21 | 2006-09-05 | Amcyte, Inc. | In situ maturation of cultured pancreatic stem cells having a specified, intermediate stage of development |
| US6881726B2 (en) * | 2001-12-24 | 2005-04-19 | Dow Pharmaceutical Sciences | Aqueous compositions containing metronidazole |
| US7141240B2 (en) * | 2002-03-12 | 2006-11-28 | Cedars-Sinai Medical Center | Glucose-dependent insulin-secreting cells transfected with a nucleotide sequence encoding GLP-1 |
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| ES2622366T3 (es) | 2009-10-26 | 2017-07-06 | F. Hoffmann-La Roche Ag | Procedimiento para la producción de una inmunoglobulina glucosilada |
| US9878071B2 (en) | 2013-10-16 | 2018-01-30 | Purdue Research Foundation | Collagen compositions and methods of use |
| CA2959139C (en) | 2014-08-27 | 2023-10-10 | Purdue Research Foundation Office Of Technology Commercialization | Collagen-based therapeutic delivery systems |
| US11919941B2 (en) | 2015-04-21 | 2024-03-05 | Purdue Research Foundation | Cell-collagen-silica composites and methods of making and using the same |
| US20180140640A1 (en) * | 2015-04-22 | 2018-05-24 | Cadens Beheer B.V. | Improved contraceptive methods and compositions and devices for use therein |
| CN106701659A (zh) * | 2016-10-31 | 2017-05-24 | 四川农业大学 | 一种猪卵巢颗粒细胞原代培养的方法 |
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| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| WO2018200750A1 (en) | 2017-04-25 | 2018-11-01 | Purdue Research Foundation | 3-dimensional (3d) tissue-engineered muscle for tissue restoration |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3882233A (en) * | 1973-07-27 | 1975-05-06 | American Home Prod | Methods of increasing production of pituitary hormones |
| US4288546A (en) * | 1976-04-09 | 1981-09-08 | The Regents Of The University Of Minnesota | Process for the large scale production of pituitary hormones by serial secondary suspension culture |
| US4439521A (en) * | 1981-10-21 | 1984-03-27 | Ontario Cancer Institute | Method for producing self-reproducing mammalian pancreatic islet-like structures |
| US4657866A (en) * | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| DK402984D0 (da) * | 1984-08-23 | 1984-08-23 | Nordisk Insulinlab | Fremgangsmaade til formering af insulin-producerende celler |
| EP0262211A1 (en) * | 1986-04-01 | 1988-04-06 | Genelabs, Incorporated | Immortalized cells which produce tissue-specific products |
| EP0283942B1 (en) * | 1987-03-24 | 1992-05-20 | W.R. Grace & Co.-Conn. | Basal nutrient medium for cell culture |
| US4929542A (en) * | 1987-10-30 | 1990-05-29 | Cornell Research Foundation, Inc. | In vitro screening test for mutagenicity and genotoxicity during spermatogenesis |
| US4980281A (en) * | 1988-02-10 | 1990-12-25 | Housey Gerard M | Method of screening for protein inhibitors and activators |
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| US4983515A (en) * | 1989-02-16 | 1991-01-08 | E. I. Du Pont De Nemours And Company | Labeled cryopreserved cells for use as targets in cytotoxicity assays |
| US4983527A (en) * | 1989-05-26 | 1991-01-08 | Arizona Board Of Regents | Oocyte test for detection of tumor promoting compounds |
| US5328844A (en) * | 1990-05-09 | 1994-07-12 | University Of Colorado Foundation, Inc. | Culture media for mammalian cells |
| JP2813467B2 (ja) * | 1993-04-08 | 1998-10-22 | ヒューマン・セル・カルチャーズ・インコーポレーテッド | 細胞培養法および培地 |
| US5534404A (en) * | 1993-12-10 | 1996-07-09 | Cytotherapeutics, Inc. | Glucose responsive insulin secreting β-cell lines and method for producing same |
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1992
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- 1992-06-23 DE DE69232535T patent/DE69232535T4/de not_active Expired - Lifetime
- 1992-06-23 EP EP92914162A patent/EP0605428B9/en not_active Expired - Lifetime
- 1992-06-23 CA CA002112112A patent/CA2112112A1/en not_active Abandoned
- 1992-06-23 ES ES92914162T patent/ES2179821T3/es not_active Expired - Lifetime
- 1992-06-23 DK DK92914162T patent/DK0605428T5/da active
- 1992-06-23 AT AT92914162T patent/ATE215605T1/de not_active IP Right Cessation
- 1992-06-23 JP JP5501606A patent/JPH06508527A/ja active Pending
- 1992-06-23 AU AU22285/92A patent/AU2228592A/en not_active Abandoned
- 1992-06-23 WO PCT/US1992/005267 patent/WO1993000441A1/en not_active Ceased
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1995
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- 1996-10-23 AU AU70365/96A patent/AU700946B2/en not_active Ceased
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1999
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| AU7036596A (en) | 1997-02-20 |
| US5928942A (en) | 1999-07-27 |
| EP0605428A4 (en) | 1995-01-11 |
| DE69232535T2 (de) | 2003-01-09 |
| DK0605428T5 (da) | 2003-01-06 |
| DE69232535T4 (de) | 2003-09-11 |
| CA2112112A1 (en) | 1993-01-07 |
| EP0605428A1 (en) | 1994-07-13 |
| AU2228592A (en) | 1993-01-25 |
| DK0605428T3 (da) | 2002-07-29 |
| EP0605428B9 (en) | 2003-01-02 |
| JPH06508527A (ja) | 1994-09-29 |
| DE69232535D1 (de) | 2002-05-08 |
| EP0605428B1 (en) | 2002-04-03 |
| ATE215605T1 (de) | 2002-04-15 |
| AU700946B2 (en) | 1999-01-14 |
| WO1993000441A1 (en) | 1993-01-07 |
| US6372493B1 (en) | 2002-04-16 |
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