ES2175992T3 - Obtencion de polimeros a partir de componentes nucleotidicos y/o no nucleotidicos por via enzimatica. - Google Patents
Obtencion de polimeros a partir de componentes nucleotidicos y/o no nucleotidicos por via enzimatica.Info
- Publication number
- ES2175992T3 ES2175992T3 ES99929296T ES99929296T ES2175992T3 ES 2175992 T3 ES2175992 T3 ES 2175992T3 ES 99929296 T ES99929296 T ES 99929296T ES 99929296 T ES99929296 T ES 99929296T ES 2175992 T3 ES2175992 T3 ES 2175992T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleotidic
- enzymatic
- components
- obtaining polymers
- polymers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229920000642 polymer Polymers 0.000 title abstract 3
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 101710086015 RNA ligase Proteins 0.000 abstract 1
- 239000000178 monomer Substances 0.000 abstract 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical group [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Macromonomer-Based Addition Polymer (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Procedimiento para la obtención de polímeros naturales y/o modificados con una secuencia definida de componentes nucleotídicos y/o no nucleotídicos, que comprende los siguientes pasos: a) unión de un cebador de la formula general p(dNp)m o p(rNp)m, pudiendo faltar, en caso dado, el grupo p en la posición 3¿ del cebador, con un soporte de la fórmula general (rM)ndN o (rM)ndU-rN, en los que p, dN, rN, m, rM, n y dU tienen los siguientes significados: p un grupo fosfato y/o fosforotioato y/o fosfonato, como preferentemente metilfosfonato y/o H-fosfonato, y/o fosforoamidato y/o formacetal y/o fosforoditioato y/o boranofosfato y/o fosforotriéster, dN un desoxiribonucleósido natural y/o modificado con bases y/o azúcares, y/o un grupo de enlace no nucleosídico apto para la unión, rN un ribonucleósido natural y/o modificado con bases y/o azúcares, y/o un grupo de enlace no nucleosídico apto para la unión, m un número entero de 1 o más, rM un ribonucleósido de purina y/o pirimidina, n un número entero de 1 o más. dU una desoxiuridina, pudiendo comprender el cebador de la fórmula general p(dNp)m o p(rNp)m también restos mixtos de dN y/o rN, con ayuda de una ARN-ligasa en presencia de cationes metálicos divalentes, no poseyendo el soporte ningún grupo protector en la posición 3¿; b) desprotección del producto de unión, en el caso de empleo del soporte de la fórmula general (rM)ndN mediante eliminación del grupo protector (rM)n en presencia de una RNasa, o bien eliminación del grupo protector (rM)ndU, en el caso de empleo del soporte de la fórmula general (rM)ndU-rN con ayuda de una uracil-ADN-glicosilasa y una endonucleasa apurínica, o eliminación de los citados grupos protectores en el intervalo alcalino; c) transferencia de un grupo terminal natural y/o modificado, de la fórmula general p definida anteriormente, al grupo 5¿-hidroxilo del producto de unión con ayuda de una polinucleótido-quinasa; d) en caso dado repetición de los pasos a)-c), y; e) en caso dado purificación del producto de unión obtenido en el paso c).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19827728 | 1998-06-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2175992T3 true ES2175992T3 (es) | 2002-11-16 |
Family
ID=7871626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES99929296T Expired - Lifetime ES2175992T3 (es) | 1998-06-22 | 1999-06-22 | Obtencion de polimeros a partir de componentes nucleotidicos y/o no nucleotidicos por via enzimatica. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6329177B1 (es) |
| EP (1) | EP1090137B1 (es) |
| JP (1) | JP2002518059A (es) |
| AT (1) | ATE215604T1 (es) |
| AU (1) | AU753605B2 (es) |
| CA (1) | CA2335562A1 (es) |
| DE (2) | DE19928591A1 (es) |
| DK (1) | DK1090137T3 (es) |
| ES (1) | ES2175992T3 (es) |
| PT (1) | PT1090137E (es) |
| WO (1) | WO1999067413A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6479262B1 (en) | 2000-05-16 | 2002-11-12 | Hercules, Incorporated | Solid phase enzymatic assembly of polynucleotides |
| WO2010094036A1 (en) | 2009-02-13 | 2010-08-19 | X-Chem, Inc. | Methods of creating and screening dna-encoded libraries |
| US20120171729A1 (en) * | 2010-12-30 | 2012-07-05 | Ibis Biosciences, Inc. | Methods for the synthesis of polyadenylic acid |
| SG10201605812YA (en) * | 2011-09-07 | 2016-09-29 | Chem Inc X | Methods for tagging dna-encoded libraries |
| KR102146721B1 (ko) | 2012-07-13 | 2020-08-21 | 엑스-켐, 인크. | 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리 |
| CN117070512B (zh) * | 2023-10-16 | 2024-04-26 | 吉林凯莱英医药化学有限公司 | tRNA及其生物合成方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3300478A (en) | 1965-06-01 | 1967-01-24 | Upjohn Co | Arabinofuranosyl 2', 5'-and 3'-5'-dinucleoside phosphates and process therefor |
| JP2780572B2 (ja) | 1991-09-13 | 1998-07-30 | 株式会社島津製作所 | オリゴヌクレオチドの酵素的合成法及びオリゴヌクレオチドのプライマーとしての使用 |
| US5516664A (en) | 1992-12-23 | 1996-05-14 | Hyman; Edward D. | Enzymatic synthesis of repeat regions of oligonucleotides |
| US5750341A (en) * | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5889136A (en) | 1995-06-09 | 1999-03-30 | The Regents Of The University Of Colorado | Orthoester protecting groups in RNA synthesis |
-
1999
- 1999-06-22 EP EP99929296A patent/EP1090137B1/de not_active Expired - Lifetime
- 1999-06-22 PT PT99929296T patent/PT1090137E/pt unknown
- 1999-06-22 DE DE19928591A patent/DE19928591A1/de not_active Withdrawn
- 1999-06-22 AU AU46144/99A patent/AU753605B2/en not_active Ceased
- 1999-06-22 CA CA002335562A patent/CA2335562A1/en not_active Abandoned
- 1999-06-22 DE DE59901131T patent/DE59901131D1/de not_active Expired - Fee Related
- 1999-06-22 AT AT99929296T patent/ATE215604T1/de not_active IP Right Cessation
- 1999-06-22 JP JP2000556053A patent/JP2002518059A/ja active Pending
- 1999-06-22 ES ES99929296T patent/ES2175992T3/es not_active Expired - Lifetime
- 1999-06-22 DK DK99929296T patent/DK1090137T3/da active
- 1999-06-22 WO PCT/EP1999/004306 patent/WO1999067413A1/de not_active Ceased
- 1999-06-22 US US09/338,124 patent/US6329177B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DK1090137T3 (da) | 2002-06-17 |
| EP1090137A1 (de) | 2001-04-11 |
| AU753605B2 (en) | 2002-10-24 |
| PT1090137E (pt) | 2002-08-30 |
| AU4614499A (en) | 2000-01-10 |
| DE19928591A1 (de) | 1999-12-23 |
| ATE215604T1 (de) | 2002-04-15 |
| DE59901131D1 (de) | 2002-05-08 |
| WO1999067413A1 (de) | 1999-12-29 |
| US6329177B1 (en) | 2001-12-11 |
| EP1090137B1 (de) | 2002-04-03 |
| CA2335562A1 (en) | 1999-12-29 |
| JP2002518059A (ja) | 2002-06-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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