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ES2031507T3 - Metodo para calibrar citometros de flujo. - Google Patents

Metodo para calibrar citometros de flujo.

Info

Publication number
ES2031507T3
ES2031507T3 ES198787306145T ES87306145T ES2031507T3 ES 2031507 T3 ES2031507 T3 ES 2031507T3 ES 198787306145 T ES198787306145 T ES 198787306145T ES 87306145 T ES87306145 T ES 87306145T ES 2031507 T3 ES2031507 T3 ES 2031507T3
Authority
ES
Spain
Prior art keywords
particles
light
calibration
instrument
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787306145T
Other languages
English (en)
Inventor
Diether J. Recktenwald
Rickie S. Kerndt
Michael R. Loken
Chia Huei Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2031507T3 publication Critical patent/ES2031507T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1012—Calibrating particle analysers; References therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1012—Calibrating particle analysers; References therefor
    • G01N2015/1014—Constitution of reference particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

UN METODO PARA CALIBRAR UN INSTRUMENTO PARA CITIMETRIA DE FLUJO, PARA UTILIZARLO EN LA OBTENCION DE AL MENOS UNA SEÑAL RELACIONADA CON LA LUZ A PARTIR DE PARTICULAS QUE PASAN A TRAVES DEL INSTRUMENTO PARA ANALISIS, METODO QUE COMPRENDE: PASAR LAS PARTICULAS DE CALIBRACION, QUE TIENEN CARACTERISTICAS RELACIONADAS CON LAS PARTICULAS A ENSAYAR, EN UNA CORRIENTE DE FLUJO LIQUIDO, DE MODO QUE CADA PARTICULA DE CALIBRACION PASA, SENCIALMENTE UNA CADA VEZ, A TRAVES DE UN HAZ DE LUZ INCIDENTE; DETECTAR UNA SEÑAL LUMINOSA PROCEDENTE DE LAS PARTICULAS DE CALIBRACION QUE PASAN A TRAVES DEL HAZ LUMINOSO; DETECTAR UNA SEÑAL DE RUIDO DE LAS PARTICULAS DE CALIBRACION QUE PASAN A TRAVES DEL HAZ DE LUZ; DETERMINAR LA RELACION DE LA SEÑAL LUMINOSA A LA SEÑAL DE RUIDO DETECTADAS; PRESENTAR LA SEÑAL COMO UN VALOR DE SEPARACION MEDIO ENTRE LAS DOS SEÑALES; COMPARAR EL VALOR DE SEPARACION MEDIO CON UN VALOR DE SEPARACION PREDETERMINADO QUE REPRESENTA UN UMBRAL PARA EL MINIMO RENDIMIENTO DEL INSTRUMENTO; Y AJUSTAR, SI NO SE HA ALCANZADO EL VALOR DE SEPARACION PREDETERMINADO, LA OPERACION DEL INSTRUMENTO, MIENTRAS SE PASAN LAS PARTICULAS DE CALIBRACION.
ES198787306145T 1986-08-29 1987-07-10 Metodo para calibrar citometros de flujo. Expired - Lifetime ES2031507T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/901,860 US4704891A (en) 1986-08-29 1986-08-29 Method and materials for calibrating flow cytometers and other analysis instruments

Publications (1)

Publication Number Publication Date
ES2031507T3 true ES2031507T3 (es) 1992-12-16

Family

ID=25414938

Family Applications (2)

Application Number Title Priority Date Filing Date
ES198787306143T Expired - Lifetime ES2035068T3 (es) 1986-08-29 1987-07-10 Kit de reactivos para ser utilizado al calibrar un instrumento para realizar analisis de fluorescencia bicromatica de particulas.
ES198787306145T Expired - Lifetime ES2031507T3 (es) 1986-08-29 1987-07-10 Metodo para calibrar citometros de flujo.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES198787306143T Expired - Lifetime ES2035068T3 (es) 1986-08-29 1987-07-10 Kit de reactivos para ser utilizado al calibrar un instrumento para realizar analisis de fluorescencia bicromatica de particulas.

Country Status (7)

Country Link
US (1) US4704891A (es)
EP (3) EP0258982B1 (es)
JP (3) JPS6363942A (es)
AT (2) ATE75042T1 (es)
DE (2) DE3778253D1 (es)
ES (2) ES2035068T3 (es)
GR (2) GR3004885T3 (es)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK164144C (da) * 1988-11-17 1992-10-12 Slagteriernes Forskningsinst Fremgangsmaade ved kontrol eller justering af maaleapparater med en optisk sonde og medium til anvendelse ved fremgangsmaaden
CA2021658C (en) * 1989-08-25 2001-10-09 Myron J. Block Multiplex immunoassay system
US5853984A (en) * 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
IE76732B1 (en) * 1990-08-07 1997-11-05 Becton Dickinson Co One step test for absolute counts
JP2913219B2 (ja) * 1991-03-06 1999-06-28 株式会社シマ研究所 尿沈渣検査成績管理方法
EP0554447B1 (en) * 1991-08-28 1997-04-09 Becton, Dickinson and Company Gravitational attractor engine for adaptively autoclustering n-dimensional data streams
US5451525A (en) * 1992-02-14 1995-09-19 Coulter Corporation Method and materials for determining particle count in a flow cytometer
EP0586183B1 (en) 1992-09-04 1999-10-13 Becton, Dickinson and Company Control particles for cell counting and instrument linearity
US5620842A (en) * 1995-03-29 1997-04-15 Becton Dickinson And Company Determination of the number of fluorescent molecules on calibration beads for flow cytometry
JP3875754B2 (ja) * 1995-11-17 2007-01-31 シスメックス株式会社 フローサイトメータ用標準液
US6014904A (en) * 1996-05-09 2000-01-18 Becton, Dickinson And Company Method for classifying multi-parameter data
DE69830598T2 (de) 1997-01-31 2006-05-18 The Horticulture And Food Research Institute Of New Zealand Limited Optische vorrichtung und methode
US6074879A (en) * 1997-06-23 2000-06-13 Bayer Corporation Synthetic polymer particles for use as standards and calibrators in flow cytometry
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6197520B1 (en) * 1999-08-13 2001-03-06 University Of Utah Research Foundation Solution-based color compensation adjusted for temperature and electronic gains
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
WO2001085914A2 (en) 2000-05-11 2001-11-15 Becton, Dickinson And Company System for identifying clusters in scatter plots using smoothed polygons with optimal boundaries
US6809804B1 (en) 2000-05-11 2004-10-26 Becton, Dickinson And Company System and method for providing improved event reading and data processing capabilities in a flow cytometer
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002043486A1 (en) 2000-11-29 2002-06-06 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
MX337304B (es) 2002-08-01 2016-02-24 Xy Llc Sistema de separacion de baja presion para celulas de esperma.
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
WO2004017041A2 (en) 2002-08-15 2004-02-26 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
CA3074799C (en) 2003-03-28 2022-12-06 Inguran, Llc Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
DK1625203T3 (en) 2003-05-15 2015-07-06 Xy Llc EFFECTIVE SEPARATION OF haploid cells FOR FLOWCYTOMETRISYSTEMER
KR101135138B1 (ko) * 2003-08-13 2012-04-16 루미넥스 코포레이션 유세포 분석기식 측정 시스템의 하나 이상의 파라미터의제어 방법
JP4850711B2 (ja) * 2003-10-02 2012-01-11 ベックマン コールター， インコーポレイテッド 血液サンプルの有核赤血球の光学測定のリファレンスコントロール
PT2151243E (pt) 2004-03-29 2013-01-25 Inguran Llc Suspensões de esperma para separação em populações enriquecidas carregando o cromossoma x ou y
JP2008507287A (ja) 2004-07-22 2008-03-13 モンサント テクノロジー エルエルシー 精子細胞の集団を富化する方法
DE102004044717B8 (de) * 2004-09-10 2010-12-16 BAM Bundesanstalt für Materialforschung und -prüfung Verfahren und Kit zur Kalibrierung eines Photolumineszenzmesssystems
JP4953710B2 (ja) * 2005-07-12 2012-06-13 シスメックス株式会社 尿中有形成分分析装置用標準物質
EP1744145B1 (en) 2005-07-12 2015-09-09 Sysmex Corporation Standard material for particle analyzer
JP4748719B2 (ja) * 2006-03-02 2011-08-17 国立大学法人大阪大学 生細胞内の特定タンパク質の定量方法および標準蛍光マイクロビーズの作製方法
CN100386054C (zh) * 2006-04-24 2008-05-07 西安交通大学 用于脉搏血氧仿真器的光散射介质及制备方法
WO2007131507A2 (en) * 2006-05-13 2007-11-22 Dako Denmark A/S Methods for flow cytometry analyses of cells without lysing subpopulations
JP5010443B2 (ja) * 2006-12-20 2012-08-29 シスメックス株式会社 血球分析装置および血球分析方法
CN101226190B (zh) * 2007-01-17 2013-07-03 深圳迈瑞生物医疗电子股份有限公司 流式细胞术的自动分类方法和装置
DE102007014413B4 (de) * 2007-03-17 2016-02-04 DüRR DENTAL AG Verfahren zum Auswerten von Fluoreszenzbildsätzen und Vorrichtung zu seiner Durchführung
US8102528B2 (en) * 2007-09-13 2012-01-24 Brightwell Technologies Inc. Particle standard and method of calibrating or validating an optical particle analyzer
WO2009042423A1 (en) * 2007-09-27 2009-04-02 Cellatope Corporation Methods and compositions for improved diagnostic assays
US8187885B2 (en) * 2009-05-07 2012-05-29 Nodality, Inc. Microbead kit and method for quantitative calibration and performance monitoring of a fluorescence instrument
US20120065092A1 (en) 2010-09-14 2012-03-15 Wai Hobert Fusion analyte cytometric bead assay, and systems and kits for performing the same
WO2012054783A2 (en) * 2010-10-21 2012-04-26 Nexcelom Bioscience Llc Internal focus reference beads for imaging cytometry
EP3217178A3 (en) 2010-11-12 2017-11-22 incellDX, Inc. Methods and systems for predicting whether a subject has a cervical intraepithelial neoplasia (cin) lesion from a suspension sample of cervical cells
JP2013146263A (ja) * 2011-12-21 2013-08-01 Azbil Corp 微生物検出装置の校正方法、及び微生物検出装置の校正キット
WO2013106772A2 (en) 2012-01-11 2013-07-18 Baxter International, Inc. Characterization of subvisible particles using a particle analyzer
GB2499796B (en) 2012-02-28 2016-06-15 Ojk Consulting Ltd Method and system for calibrating a flow cytometer
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
ITTV20130026A1 (it) * 2013-02-27 2014-08-28 Texa Spa Metodo e sistema per calibrare uno strumento analizzatore di particolato di un gas
EP3016968B1 (en) 2013-07-01 2019-06-26 Newsouth Innovations Pty Limited Diagnosis and treatment of autoimmune diseases
US20170248511A1 (en) 2014-12-04 2017-08-31 Becton, Dickinson And Company Flow Cytometry Cell Sorting Systems and Methods of Using the Same
CN208443705U (zh) 2014-12-10 2019-01-29 贝克顿·迪金森公司 光学对准的集光系统和测量系统
KR102656644B1 (ko) 2015-02-09 2024-04-09 슬링샷 바이오사이언시즈 인코포레이티드 튜닝가능한 광 특성을 갖는 하이드로겔 입자 및 이를 사용하기 위한 방법
ES2987551T3 (es) 2015-02-18 2024-11-15 Becton Dickinson Co Sistemas de detección óptica y métodos de uso de los mismos
CN107428918B (zh) 2015-03-12 2021-07-30 贝克顿·迪金森公司 紫外线吸收聚合物染料及其使用方法
EP3268435B1 (en) 2015-03-12 2022-07-13 Becton, Dickinson and Company Polymeric bodipy dyes and methods for using the same
ES2965719T3 (es) 2015-06-17 2024-04-16 Becton Dickinson Co Unidad de tapón de dispersión de detector óptico que tiene una barra de dispersión desmontable y métodos para usar la misma
EP3322969B1 (en) * 2015-07-15 2020-03-04 Becton, Dickinson and Company System and method for adjusting cytometer measurements
US10876953B2 (en) 2015-07-15 2020-12-29 Becton, Dickinson And Company System and method for label selection
CN108474728B (zh) 2015-10-13 2022-04-26 贝克顿·迪金森公司 多模态荧光成像流式细胞术系统
EP3394179B1 (en) 2015-12-16 2022-03-16 Becton, Dickinson and Company Photostable fluorescent polymeric tandem dyes including luminescent metal complexes
US20190086319A1 (en) 2016-03-10 2019-03-21 Becton, Dickinson And Company Methods of evaluating a cellular sample for her-2/neu expression and compositions for practicing the same
JP6237806B2 (ja) * 2016-03-16 2017-11-29 ソニー株式会社 微小粒子分取装置
JP7130562B2 (ja) 2016-03-17 2022-09-05 ベクトン・ディキンソン・アンド・カンパニー ハイスループット蛍光フローサイトメーターを用いる細胞選別
GB2548807A (en) * 2016-03-23 2017-10-04 Sony Corp Information processing apparatus,second information processing apparatus, system,method and computer program product
CN105928753B (zh) * 2016-04-18 2019-01-15 中国科学院苏州生物医学工程技术研究所 流式细胞仪校准用鸡红细胞的制备方法
CN109070083A (zh) 2016-04-22 2018-12-21 贝克顿迪金森公司 用于阵列产生的高密度沉积
KR102381176B1 (ko) 2016-05-12 2022-04-01 비디 바이오사이언시스 개선된 이미지 해상도를 갖는 형광 이미징 유세포 분석법
CN118530607A (zh) 2016-07-07 2024-08-23 贝克顿·迪金森公司 发荧光的水溶剂化共轭聚合物
ES2951466T3 (es) 2016-09-13 2023-10-23 Becton Dickinson Co Citómetro de flujo con ecualización óptica
US10732094B2 (en) 2016-10-03 2020-08-04 Becton, Dickinson And Company Methods and systems for determining a drop delay of a flow stream in a flow cytometer
ES2950436T3 (es) 2016-12-14 2023-10-10 Becton Dickinson Co Métodos y composiciones para obtener una valoración de tuberculosis en un sujeto
JP7058662B2 (ja) 2017-02-08 2022-04-22 ベクトン・ディキンソン・アンド・カンパニー 乾燥色素試薬装置、ならびにその製造及び使用方法
WO2018156371A1 (en) 2017-02-27 2018-08-30 Becton, Dickinson And Company Light detection systems and methods for using thereof
JP6955385B2 (ja) * 2017-07-14 2021-10-27 株式会社堀場製作所 粒子分析装置における光照射を調整するためのモニター装置
CN111758019B (zh) 2018-01-23 2024-06-28 贝克顿·迪金森公司 用于动态光检测遮蔽的系统及其使用方法
CN119220109A (zh) 2018-03-30 2024-12-31 贝克顿·迪金森公司 含侧基发色团的水溶性聚合染料
WO2019209713A1 (en) 2018-04-27 2019-10-31 Becton, Dickinson And Company Collection systems for flow cytometrically sorted samples and methods of using the same
EP3811055A4 (en) 2018-06-19 2022-08-10 Becton, Dickinson and Company VARIABLE MULTIPLEXING SWITCHES FOR SENSOR NETWORKS, SYSTEMS AND METHODS OF USE THEREOF
CN119845832A (zh) 2019-03-22 2025-04-18 贝克顿·迪金森公司 使用射频多路复用激发数据对荧光成像进行光谱解混
CA3165821A1 (en) 2020-01-24 2021-07-29 Jeffrey Kim Compositions and methods for cell-like calibration particles
CN115176138A (zh) * 2020-02-24 2022-10-11 康宁股份有限公司 包含具有尼龙-6和染料的纳米颗粒的校准装置
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
JP2023524524A (ja) 2020-05-04 2023-06-12 スリングショット バイオサイエンシーズ， インコーポレイテッド 多重化アッセイのためのパッシブ光学バーコード付与のための組成物および方法
EP4327070A4 (en) 2021-04-23 2024-08-14 Becton, Dickinson and Company FLUID MANAGEMENT SYSTEM FOR A FLOW TYPE ANALYZER AND/OR SORTER PARTICLE ANALYZER
EP4155349A1 (en) 2021-09-24 2023-03-29 Becton, Dickinson and Company Water-soluble yellow green absorbing dyes
WO2023076629A1 (en) 2021-10-29 2023-05-04 Slingshot Biosciences, Inc. Hydrogel particles as feeder cells and as synthetic antigen presenting cells
WO2023215886A1 (en) 2022-05-05 2023-11-09 Slingshot Biosciences, Inc. Engineered particles as red blood cell mimics and compositions containing same for hematology
EP4531813A1 (en) 2022-06-02 2025-04-09 Slingshot Biosciences, Inc. Apoptotic cell mimic
WO2024092161A2 (en) 2022-10-26 2024-05-02 Slingshot Biosciences, Inc. Size-tunable synthetic particles with tunable optical properties and methods for using the same for immune cell activation
WO2025049609A1 (en) 2023-08-29 2025-03-06 Slingshot Biosciences, Inc. Cd34 stem cell mimics
US20250137905A1 (en) 2023-10-27 2025-05-01 Becton, Dickinson And Company Fluid Supply Systems Having a Flow Control Circuit and Single Fluidic Connection, and Methods of Use Thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3406121A (en) * 1965-10-01 1968-10-15 Dade Reagents Inc Blood cell counting standard and method of preparing the same
US3412037A (en) * 1966-01-26 1968-11-19 Technicon Corp Method and means for the calibration of particle counting apparatus
US3791517A (en) * 1973-03-05 1974-02-12 Bio Physics Systems Inc Digital fluidic amplifier particle sorter
US4135821A (en) * 1977-03-22 1979-01-23 The United States Of America As Represented By The United States Department Of Energy Calibration of optical particle-size analyzer
US4331862A (en) * 1979-02-23 1982-05-25 Ryan Wayne L Method for calibrating a particle counting machine and a calibration standard therefor
US4380392A (en) * 1981-03-18 1983-04-19 Karabegov Mikhail A Method and apparatus for calibration of instruments serving to count and to determine the size of particles suspended in dispersion medium
US4438390A (en) * 1981-03-23 1984-03-20 Coulter Electronics, Inc. Tandem sensing zones for improved signal-to-noise ratio in particle analyzer
US4434647A (en) * 1981-07-27 1984-03-06 Lockheed Corporation Dynamic spot calibration for automatic particle counters
US4475236A (en) * 1981-11-12 1984-10-02 Ortho Diagnostic Systems Inc. Method for counting overlapping cell populations in a distribution histogram
US4499052A (en) * 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
JPS59184862A (ja) * 1983-04-05 1984-10-20 ベクトン・ディッキンソン・アンド・カンパニ− 試料内細胞の複数副次集団を識別する方法と装置
US4596464A (en) * 1983-10-14 1986-06-24 Ortho Diagnostic Systems, Inc. Screening method for red cell abnormality

Also Published As

Publication number Publication date
EP0258982A2 (en) 1988-03-09
GR3006407T3 (es) 1993-06-21
EP0258983A2 (en) 1988-03-09
EP0258982B1 (en) 1992-09-23
ES2035068T3 (es) 1993-04-16
DE3778253D1 (de) 1992-05-21
ATE80947T1 (de) 1992-10-15
GR3004885T3 (es) 1993-04-28
ATE75042T1 (de) 1992-05-15
JPS63177038A (ja) 1988-07-21
EP0258982A3 (en) 1989-10-25
US4704891A (en) 1987-11-10
JPS63171343A (ja) 1988-07-15
EP0257759A3 (en) 1989-10-18
DE3781855T2 (de) 1993-04-29
JPH0350211B2 (es) 1991-08-01
EP0257759A2 (en) 1988-03-02
JPH0435709B2 (es) 1992-06-11
EP0258983B1 (en) 1992-04-15
JPH0350212B2 (es) 1991-08-01
JPS6363942A (ja) 1988-03-22
DE3781855D1 (de) 1992-10-29
EP0258983A3 (en) 1989-09-13

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