ES2025654T3 - Un metodo para la deteccion de un ligando en un fluido sospechoso de contener el ligando. - Google Patents
Un metodo para la deteccion de un ligando en un fluido sospechoso de contener el ligando.Info
- Publication number
- ES2025654T3 ES2025654T3 ES198787303661T ES87303661T ES2025654T3 ES 2025654 T3 ES2025654 T3 ES 2025654T3 ES 198787303661 T ES198787303661 T ES 198787303661T ES 87303661 T ES87303661 T ES 87303661T ES 2025654 T3 ES2025654 T3 ES 2025654T3
- Authority
- ES
- Spain
- Prior art keywords
- league
- fluid
- catalyst
- modulator
- indicator reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012530 fluid Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 239000003054 catalyst Substances 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/821—Chemistry: analytical and immunological testing involving complement factors or complement systems
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
UN METODO DE INMUNOENSAYO PARA UN LIGANDO SOSPECHOSO DE ESTAR PRESENTE EN UN FLUIDO INCLUYE EL USO DE UN ENZIMA, UN CATALIZADOR ION METALICO PARA UNA REACCION DE INDICADOR Y UN MODULADOR DE BLOQUEO PARA EL CATALIZADOR. EL LIGANDO PRESENTE EN EL FLUIDO SE UNE A UN ANTILIGANDO. LA FRACCION DE ENLACE RESULTANTE ACTIVA EL ENZIMA PARA DESBLOQUEAR EL MODULADOR. EL MODULADOR LIBRE ACTIVA O INHIBE EL CATALIZADOR, MODULANDO ASI LAS VELOCIDADES DE UNA REACCION INDICADOR ENTRE UN SUBSTRATO Y UN REACTIVO REDOX. LA PRESENCIA O AUSENCIA DEL LIGANDO EN EL FLUIDO SE INDICA POR UNA SEÑAL, TAL COMO UN CAMBIO DE COLOR O UNA VELOCIDAD DE CAMBIO DE COLOR, CONSECUENTE A LA REACCION INDICADOR. LA INVENCION TAMBIEN INCLUYE UN JUEGO DE MATERIALES UTILES PARA LLEVAR A CABO EL METODO DE LA INVENCION.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/861,818 US4810631A (en) | 1986-05-12 | 1986-05-12 | Signal enhancement in immunoassay by modulation of chemical catalysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2025654T3 true ES2025654T3 (es) | 1992-04-01 |
Family
ID=25336850
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES198787303661T Expired - Lifetime ES2025654T3 (es) | 1986-05-12 | 1987-04-24 | Un metodo para la deteccion de un ligando en un fluido sospechoso de contener el ligando. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US4810631A (es) |
| EP (1) | EP0245981B1 (es) |
| JP (1) | JPH063447B2 (es) |
| AU (1) | AU596832B2 (es) |
| BR (1) | BR8701435A (es) |
| CA (1) | CA1271419A (es) |
| DE (1) | DE3772840D1 (es) |
| DK (1) | DK233987A (es) |
| ES (1) | ES2025654T3 (es) |
| MX (1) | MX167488B (es) |
| NZ (1) | NZ219442A (es) |
| ZA (1) | ZA871750B (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4962024A (en) * | 1988-08-11 | 1990-10-09 | Becton, Dickinson And Company | Signal enhancement in assay for an enzyme |
| CA2000678A1 (en) * | 1988-11-17 | 1990-05-17 | John E. Reardon | Chromogenic substrate for esterase and immunoassay using same |
| IE900688A1 (en) * | 1989-03-08 | 1991-02-13 | Becton Dickinson Co | Signal enhancement in magnetic immunoassay by inhibition of¹enzymatic catalysis |
| US5196306A (en) * | 1989-03-29 | 1993-03-23 | E. I. Du Pont De Nemours And Company | Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system |
| GB8927503D0 (en) * | 1989-12-04 | 1990-02-07 | Kronem Systems Inc | Enzyme-amplified lanthanide chelate luminescence |
| US5972630A (en) * | 1991-08-19 | 1999-10-26 | Dade Behring Marburg Gmbh | Homogeneous immunoassays using enzyme inhibitors |
| US5279937A (en) * | 1992-04-30 | 1994-01-18 | Detechnology Canada | Use of macroglobulins to improve the signal-to-background ratio in affinity binding assays |
| US5843666A (en) * | 1994-09-02 | 1998-12-01 | Lumigen, Inc. | Chemiluminescent detection methods using dual enzyer-labeled binding partners |
| WO2001056456A2 (en) * | 2000-02-02 | 2001-08-09 | Cygene, Inc. | Methods and compositions for detection of disease |
| EP1332357B1 (en) | 2000-10-14 | 2006-05-24 | Alex M. Saunders | Ligand based solution assay for low concentration analytes |
| GB0310270D0 (en) * | 2003-05-03 | 2003-06-11 | Univ Edinburgh | Biomolecular devices |
| JP2009517690A (ja) * | 2005-11-30 | 2009-04-30 | エージェンシー フォー サイエンス， テクノロジー アンド リサーチ | 分析物を検出する電気化学的方法 |
| US9945133B2 (en) * | 2011-11-15 | 2018-04-17 | New Standards Manufacturing Co. | System and method for aligning and leveling tile |
| RU2713112C2 (ru) | 2013-04-01 | 2020-02-03 | Бектон, Дикинсон Энд Компани | Способы и средства для диагностики гриппа |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4160645A (en) * | 1977-07-14 | 1979-07-10 | Syva Company | Catalyst mediated competitive protein binding assay |
| US4233144A (en) * | 1979-04-16 | 1980-11-11 | Technicon Instruments Corporation | Electrode for voltammetric immunoassay |
| US4446231A (en) * | 1979-10-03 | 1984-05-01 | Self Colin H | Immunoassay using an amplified cyclic detection system |
| GB2059421A (en) * | 1979-10-03 | 1981-04-23 | Self C H | Assay method and reagents therefor |
| US4375972A (en) * | 1979-10-17 | 1983-03-08 | Allied Corporation | Heterogeneous chemiluminescent immunoassays utilizing metallo porphyrin tag |
| US4463090A (en) * | 1981-09-30 | 1984-07-31 | Harris Curtis C | Cascade amplification enzyme immunoassay |
| GB8432069D0 (en) * | 1984-12-19 | 1985-01-30 | Iq Bio Ltd | Apparatus for immunoassay |
| US4865972A (en) * | 1986-08-22 | 1989-09-12 | Westinghouse Electric Corp. | Antibody-based bioassay for enzyme-inducing chemicals |
-
1986
- 1986-05-12 US US06/861,818 patent/US4810631A/en not_active Expired - Fee Related
-
1987
- 1987-02-27 CA CA000530772A patent/CA1271419A/en not_active Expired - Fee Related
- 1987-03-02 NZ NZ219442A patent/NZ219442A/xx unknown
- 1987-03-11 ZA ZA871750A patent/ZA871750B/xx unknown
- 1987-03-23 AU AU70500/87A patent/AU596832B2/en not_active Ceased
- 1987-03-30 BR BR8701435A patent/BR8701435A/pt not_active Application Discontinuation
- 1987-04-24 DE DE8787303661T patent/DE3772840D1/de not_active Expired - Fee Related
- 1987-04-24 EP EP87303661A patent/EP0245981B1/en not_active Expired - Lifetime
- 1987-04-24 ES ES198787303661T patent/ES2025654T3/es not_active Expired - Lifetime
- 1987-05-07 DK DK233987A patent/DK233987A/da unknown
- 1987-05-11 MX MX006433A patent/MX167488B/es unknown
- 1987-05-12 JP JP62115718A patent/JPH063447B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| BR8701435A (pt) | 1988-01-26 |
| DK233987A (da) | 1987-11-13 |
| US4810631A (en) | 1989-03-07 |
| ZA871750B (en) | 1987-08-31 |
| EP0245981A3 (en) | 1988-05-25 |
| EP0245981A2 (en) | 1987-11-19 |
| EP0245981B1 (en) | 1991-09-11 |
| DE3772840D1 (de) | 1991-10-17 |
| JPS62274261A (ja) | 1987-11-28 |
| DK233987D0 (da) | 1987-05-07 |
| JPH063447B2 (ja) | 1994-01-12 |
| MX167488B (es) | 1993-03-25 |
| CA1271419A (en) | 1990-07-10 |
| AU7050087A (en) | 1987-11-19 |
| AU596832B2 (en) | 1990-05-17 |
| NZ219442A (en) | 1989-03-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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