ES2013572A6 - Method for the determination of lipid-bound sialic acid content of blood - Google Patents
Method for the determination of lipid-bound sialic acid content of bloodInfo
- Publication number
- ES2013572A6 ES2013572A6 ES8902167A ES8902167A ES2013572A6 ES 2013572 A6 ES2013572 A6 ES 2013572A6 ES 8902167 A ES8902167 A ES 8902167A ES 8902167 A ES8902167 A ES 8902167A ES 2013572 A6 ES2013572 A6 ES 2013572A6
- Authority
- ES
- Spain
- Prior art keywords
- lipid
- sialic acid
- bound sialic
- solution
- acid content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 150000002632 lipids Chemical class 0.000 title abstract 5
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 title abstract 4
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 title abstract 4
- 210000004369 blood Anatomy 0.000 title abstract 3
- 239000008280 blood Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- 230000001376 precipitating effect Effects 0.000 abstract 3
- 238000001556 precipitation Methods 0.000 abstract 3
- 102000004895 Lipoproteins Human genes 0.000 abstract 2
- 108090001030 Lipoproteins Proteins 0.000 abstract 2
- 239000003791 organic solvent mixture Substances 0.000 abstract 2
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical class [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 abstract 1
- WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical class [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 abstract 1
- 239000002202 Polyethylene glycol Substances 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000008346 aqueous phase Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
- 239000011572 manganese Substances 0.000 abstract 1
- 239000011707 mineral Substances 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 229920001223 polyethylene glycol Polymers 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57469—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving tumor associated glycolinkage, i.e. TAG
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a method for the determination of lipid-bound sialic acid content of blood, primarily of human blood, in which serum is diluted with water, then extracted with a water-immiscible organic solvent or solvent mixture to remove neutral lipids, a lipoprotein fraction containing lipid-bound sialic acid is precipitated from the aqueous phase, the precipitated lipoprotein fraction is redissolved, a mineral acid, a copper(II) salt and resorcine are added to the solution, and the lipid-bound sialic acid content of the serum is calculated on the basis of the colour reaction produced with resorcine. According to the invention a) dextrane with a molecular weight of 10,000 to 20,000 is applied as precipitating agent in a solution with a concentration of 15-35 mg/100 ml and precipitation is performed at a pH of 5.8 to 7.5, or b) polyethylene glycol with a molecular weight of 4,000 to 10,000 is applied as precipitating agent in a solution with a concentration of 15-55 g/100 ml and precipitation is performed at a pH of 9 to 11, or c) a water-soluble manganese(II) salt is applied as precipitating agent in a solution corresponding to a Mn<2+> ion concentration of 8.7-13 g/100 ml and precipitation is performed at a pH of 5.5 to 7.5.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU89885A HU204131B (en) | 1989-02-24 | 1989-02-24 | Method for detecting sial acidcontent adherent to the lipid of the blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2013572A6 true ES2013572A6 (en) | 1990-05-01 |
Family
ID=10951876
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES8902167A Expired - Fee Related ES2013572A6 (en) | 1989-02-24 | 1989-06-21 | Method for the determination of lipid-bound sialic acid content of blood |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPH02242160A (en) |
| KR (1) | KR920700401A (en) |
| CN (1) | CN1045185A (en) |
| AU (1) | AU3697189A (en) |
| ES (1) | ES2013572A6 (en) |
| HU (1) | HU204131B (en) |
| WO (1) | WO1990010225A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5462877A (en) * | 1993-11-05 | 1995-10-31 | Katopodis; Nonda | Method for determining lipid bound sialic acid in plasma or serum |
| AU8483698A (en) * | 1997-07-15 | 1999-02-10 | Bioprobes, Inc. | Determining alcohol intake using sialic acid/apo j |
| CA3125914A1 (en) * | 2019-01-07 | 2020-07-16 | 1866402 Ontario Limited | Blood separation and analysis device and methods |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4342567A (en) * | 1981-07-06 | 1982-08-03 | Sloan Kettering Institute For Cancer Research | Sialic acid determination method |
| JPH0775557B2 (en) * | 1986-04-25 | 1995-08-16 | 富士レビオ株式会社 | Method for measuring lipid-bound sialic acid |
-
1989
- 1989-02-24 HU HU89885A patent/HU204131B/en not_active IP Right Cessation
- 1989-05-22 AU AU36971/89A patent/AU3697189A/en not_active Abandoned
- 1989-05-22 WO PCT/HU1989/000022 patent/WO1990010225A1/en not_active Ceased
- 1989-05-22 KR KR1019900702324A patent/KR920700401A/en not_active Withdrawn
- 1989-06-21 ES ES8902167A patent/ES2013572A6/en not_active Expired - Fee Related
- 1989-06-27 CN CN89104231A patent/CN1045185A/en active Pending
- 1989-07-04 JP JP1171347A patent/JPH02242160A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN1045185A (en) | 1990-09-05 |
| WO1990010225A1 (en) | 1990-09-07 |
| JPH02242160A (en) | 1990-09-26 |
| KR920700401A (en) | 1992-02-19 |
| AU3697189A (en) | 1990-09-26 |
| HU204131B (en) | 1991-11-28 |
| HUT53227A (en) | 1990-09-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD1A | Patent lapsed |
Effective date: 19990405 |