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ES2097363T3 - Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas. - Google Patents

Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas.

Info

Publication number
ES2097363T3
ES2097363T3 ES92922343T ES92922343T ES2097363T3 ES 2097363 T3 ES2097363 T3 ES 2097363T3 ES 92922343 T ES92922343 T ES 92922343T ES 92922343 T ES92922343 T ES 92922343T ES 2097363 T3 ES2097363 T3 ES 2097363T3
Authority
ES
Spain
Prior art keywords
transcription
euchariot
excerpt
exempt
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92922343T
Other languages
English (en)
Inventor
David V Thompson
Oosbree Thomas R Van
Gregory S Beckler
Herwynen John F Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25103433&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2097363(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ES2097363T3 publication Critical patent/ES2097363T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

UN METODO PARA LIGAR LA TRANSCRIPCION Y TRANSLACION DE UN ADN UTILIZANDO UNA SOLUCION QUE COMPRENDE UN EXTRACTO LIBRE DE CELULAS CONSISTE EN LA ADICION DE UNA CANTIDAD SUFICIENTE DE UN COMPUESTO DE MAGNESIO AL EXTRACTO PARA AUMENTAR LA CONCENTRACION DE MAGNESIO A UN NIVEL DONDE EL ARN SE TANSCRIBE DESDE EL ADN Y SE TRASLADA A UNA PROTEINA.
ES92922343T 1991-10-11 1992-10-07 Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas. Expired - Lifetime ES2097363T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US77513691A 1991-10-11 1991-10-11

Publications (1)

Publication Number Publication Date
ES2097363T3 true ES2097363T3 (es) 1997-04-01

Family

ID=25103433

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92922343T Expired - Lifetime ES2097363T3 (es) 1991-10-11 1992-10-07 Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas.

Country Status (10)

Country Link
US (1) US5324637A (es)
EP (1) EP0566714B1 (es)
JP (1) JP2904583B2 (es)
AT (1) ATE147104T1 (es)
AU (1) AU660329B2 (es)
DE (1) DE69216385T2 (es)
DK (1) DK0566714T3 (es)
ES (1) ES2097363T3 (es)
GR (1) GR3022719T3 (es)
WO (1) WO1993007287A1 (es)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
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US5665563A (en) * 1991-10-11 1997-09-09 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
US5492817A (en) * 1993-11-09 1996-02-20 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
AU4843893A (en) * 1992-09-02 1994-03-29 Scripps Research Institute, The Coupled isothermal polynucleotide amplification and translation system
AU4930993A (en) * 1992-09-24 1994-04-12 Public Health Research Institute Of The City Of New York, Inc., The Coupled replication-translation methods and kits for protein synthesis
KR0131166B1 (ko) * 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법
US5874231A (en) * 1994-08-22 1999-02-23 Mcgill University Methods of screening for non-hormone compounds which effect modulation of polypeptide translation
EP0795027A4 (en) * 1994-11-08 1999-03-10 Novagen Inc IN VITRO SYNTHESIS OF PROTEINS
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
FI990655A7 (fi) 1996-10-01 1999-06-01 Geron Corp Ihmistelomeraasin katalyyttinen alayksikkö
WO1998035062A1 (en) * 1997-02-07 1998-08-13 Lingappa Jaisri R Multistep, atp-dependent cell-free system for the assembly of human immunodeficiency virus capsids
US7638269B2 (en) * 1997-02-07 2009-12-29 The Regents Of The University Of California Viral capsid assembly intermediates and methods of production
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6168931B1 (en) 1999-03-17 2001-01-02 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of biological macromolecules using a novel ATP regeneration system
US6994986B2 (en) 1999-03-17 2006-02-07 The Board Of Trustees Of The Leland Stanford University In vitro synthesis of polypeptides by optimizing amino acid metabolism
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
KR100546009B1 (ko) * 1999-05-11 2006-01-25 셀프리 사이언스 코 엘티디 무세포 단백질 합성용 세포추출물 함유제제 및 무세포단백질 합성수단
US20030091999A1 (en) * 1999-10-01 2003-05-15 Zhongping Yu Compositions and methods for identifying polypeptides and nucleic acid molecules
US6632645B1 (en) * 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
ATE286140T1 (de) * 2000-05-03 2005-01-15 Emd Biosciences Inc E. coli extrakt zur synthese von proteinen
US6878861B2 (en) * 2000-07-21 2005-04-12 Washington State University Research Foundation Acyl coenzyme A thioesterases
KR100399337B1 (ko) * 2001-02-07 2003-09-26 드림바이오젠 주식회사 단백질의 무세포 번역후수식법
JP4328530B2 (ja) * 2001-03-08 2009-09-09 ライフ テクノロジーズ コーポレーション 改良インビトロ合成系
WO2003023360A2 (en) * 2001-09-10 2003-03-20 Meso Scale Technologies, Llc Methods and apparatus for conducting multiple measurements on a sample
US8034581B2 (en) * 2001-11-26 2011-10-11 Toshio Hara Cell-free extract and glycoprotein synthesis system
EP1477566B1 (en) * 2002-01-31 2007-09-26 CellFree Sciences Co., Ltd. Germ extract for cell-free protein synthesis and process for producing the same
US7744816B2 (en) 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
US8278055B2 (en) * 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
ATE411400T1 (de) 2002-11-21 2008-10-15 Epict Technologies Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
US7799937B2 (en) * 2004-10-25 2010-09-21 Synthonics, Inc. Metal coordinated compositions
US8779175B2 (en) 2004-10-25 2014-07-15 Synthonics, Inc. Coordination complexes, pharmaceutical solutions comprising coordination complexes, and methods of treating patients
US20060141054A1 (en) 2004-10-25 2006-06-29 Thomas Piccariello Metal coordinated compositions
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
WO2008106660A2 (en) * 2007-03-01 2008-09-04 Invitrogen Corporation Isolated phospholipid-protein particles
US8680019B2 (en) * 2007-08-10 2014-03-25 Protelica, Inc. Universal fibronectin Type III binding-domain libraries
US8470966B2 (en) 2007-08-10 2013-06-25 Protelica, Inc. Universal fibronectin type III binding-domain libraries
US20090176654A1 (en) * 2007-08-10 2009-07-09 Protelix, Inc. Universal fibronectin type III binding-domain libraries
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
CA2697193C (en) 2007-09-14 2017-06-06 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor
ES2860453T3 (es) 2009-10-30 2021-10-05 Novartis Ag Bibliotecas universales del dominio de unión del lado inferior de la fibronectina de tipo III
US20130203110A1 (en) 2010-04-20 2013-08-08 Institut Fuer Mikrotechnik Mainz Gmbh System for the in vitro transcription and translation of membrane proteins
WO2012009568A2 (en) 2010-07-16 2012-01-19 Adimab, Llc Antibody libraries
AU2011283646B2 (en) 2010-07-30 2015-07-09 Novartis Ag Fibronectin cradle molecules and libraries thereof
US10684289B2 (en) 2013-12-09 2020-06-16 Adimab, Llc Polyclonal mixtures of antibodies, and methods of making and using them
EP3280444B1 (en) 2015-04-10 2024-05-08 Adimab, LLC Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species
JP7636135B2 (ja) * 2017-02-09 2025-02-26 コルテバ アグリサイエンス エルエルシー 人工エネルギー再生システムを必要としない新規の無真核細胞発現システム
CN113409887A (zh) * 2021-07-07 2021-09-17 中国科学院生物物理研究所 一种无细胞翻译体系、方法及产物

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1705302A1 (ru) * 1988-12-22 1992-01-15 Институт Белка Ан Ссср Способ препаративной экспрессии генов в бесклеточной системе сопр женной транскрипции/трансл ции
EP0593757B1 (en) * 1989-07-31 1997-01-15 Institute Of Protein Research, Russian Academy Of Sciences Method for obtaining polypeptides in a cell-free system

Also Published As

Publication number Publication date
AU660329B2 (en) 1995-06-22
US5324637A (en) 1994-06-28
EP0566714A1 (en) 1993-10-27
EP0566714A4 (en) 1994-07-13
GR3022719T3 (en) 1997-06-30
ATE147104T1 (de) 1997-01-15
AU2792192A (en) 1993-05-03
JPH06503477A (ja) 1994-04-21
DE69216385D1 (de) 1997-02-13
DK0566714T3 (da) 1997-06-16
WO1993007287A1 (en) 1993-04-15
JP2904583B2 (ja) 1999-06-14
EP0566714B1 (en) 1997-01-02
DE69216385T2 (de) 1997-06-12

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