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EP4650358A2 - Analogue de coiffe modifié par un ribose et son utilisation - Google Patents

Analogue de coiffe modifié par un ribose et son utilisation

Info

Publication number
EP4650358A2
EP4650358A2 EP25158385.2A EP25158385A EP4650358A2 EP 4650358 A2 EP4650358 A2 EP 4650358A2 EP 25158385 A EP25158385 A EP 25158385A EP 4650358 A2 EP4650358 A2 EP 4650358A2
Authority
EP
European Patent Office
Prior art keywords
cap
mmol
ribose
solvate
stereoisomer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP25158385.2A
Other languages
German (de)
English (en)
Inventor
Honglei Zhang
Gengshen SONG
Yangjian LIU
Kai DONG
Yuqing MA
Chao Zhang
Yanfeng Wang
Guoliang Zhang
Lili Jiang
Jinyu Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Youcare Kechuang Pharmaceutical Technology Co Ltd
Original Assignee
Beijing Youcare Kechuang Pharmaceutical Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN202410814191.9A external-priority patent/CN118389495B/zh
Application filed by Beijing Youcare Kechuang Pharmaceutical Technology Co Ltd filed Critical Beijing Youcare Kechuang Pharmaceutical Technology Co Ltd
Publication of EP4650358A2 publication Critical patent/EP4650358A2/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/45—Transferases (2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
    • A61K48/0033—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid the non-active part being non-polymeric
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A61K9/5107—Excipients; Inactive ingredients
    • A61K9/5123—Organic compounds, e.g. fats, sugars
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A61K9/5107—Excipients; Inactive ingredients
    • A61K9/513—Organic macromolecular compounds; Dendrimers
    • A61K9/5146—Organic macromolecular compounds; Dendrimers obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01001—Inorganic diphosphatase (3.6.1.1)

Definitions

  • the present disclosure belongs to the technical field of chemical and biological engineering, and relates to a ribose-modified cap analog and a use thereof.
  • cap structure is a special structure at the 5' end of mRNA formed by modification during mRNA transcription, namely an m7GPPPN structure, also known as a methylguanosine cap. It is formed under the co-catalysis of RNA triphosphatase, guanylyltransferase, mRNA (guanine-N7) methyltransferase, and mRNA (nucleoside-2') methyltransferase.
  • CAP0 CAP1
  • CAP2 CAP2
  • m7G5'ppp5'Np m7G5'ppp5'NmpNp
  • m7G5'ppp5'NmpNmpNp m7G5'ppp5'NmpNp
  • the cap structure is necessary for the initiation of mRNA translation, which provides a signal for the recognition of mRNA by the ribosome, assists the ribosome in binding to the mRNA, and enables translation to start from AUG. Meanwhile, the cap structure can increase the stability of mRNA and protect the mRNA from 5'-3' exonuclease attack.
  • cap structure is like a steel helmet for mRNA, which can not only protect the mRNA from being destroyed, but also imprint the helmet through chemical modification to facilitate recognition by other members.
  • cap structure analogs are also mostly used to improve the stability of mRNA structures during in vitro transcription, with ARCA and Cap1 structure analogs being the common ones.
  • the cap structure of mRNA is importantly linked to mRNA quality control and innate immunity of the organism. Therefore, the invention of a novel cap analog is of great significance for increasing the stability of mRNA and improving the translation efficiency of mRNA.
  • the present disclosure aims to provide a ribose-modified cap analog and a use thereof.
  • the ribose-modified cap analog of the present disclosure can improve the stability of mRNA and/or the translation efficiency of mRNA.
  • a first aspect of the present disclosure provides a ribose-modified cap analog, or a stereoisomer, a pharmaceutically acceptable salt, or a solvate thereof, wherein the ribose-modified cap analog has a structure of formula (I):
  • the ribose-modified cap analogs of the present disclosure can result in a significant increase in the in vitro transcription yield of mRNA, the capping rate, the translation efficiency of mRNA, and the amount and duration of protein expression by mRNA in mice, as well as a significant decrease in the decapping rate.
  • a second aspect of the present disclosure provides a use of the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure in the preparation of an in vitro co-transcription mRNA capping reagent.
  • a third aspect of the present disclosure provides an RNA molecule comprising the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure as a cap structure or a cap structure fragment.
  • a fourth aspect of the present disclosure provides a pharmaceutical composition comprising the RNA molecule according to the third aspect of the present disclosure.
  • a fifth aspect of the present disclosure provides a method for synthesizing an mRNA molecule for non-disease diagnostic and therapeutic purposes comprising the steps of: co-incubating the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure with a polynucleotide template for template transcription.
  • a sixth aspect of the present disclosure provides a capped mRNA transcription reaction system for non-disease diagnostic and therapeutic purposes comprising:
  • a seventh aspect of the present disclosure provides a kit comprising: (1) the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure; and (2) a nucleotide triphosphate molecule and an RNA polymerase.
  • An eighth aspect of the present disclosure provides a method for improving intracellular stability of an RNA, comprising incorporating the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure into the RNA.
  • a ninth aspect of the present disclosure provides a method for introducing RNA into a cell, comprising contacting the cell with the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure, or the pharmaceutical composition according to the fourth aspect of the present disclosure.
  • a tenth aspect of the present disclosure provides a method for inhibiting RNA translation in a cell, comprising contacting the cell with the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure, or the pharmaceutical composition according to the fourth aspect of the present disclosure.
  • An eleventh aspect of the present disclosure provides a use of the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure, or the pharmaceutical composition according to the fourth aspect of the present disclosure in the preparation of a vaccine.
  • the ribose-modified cap analogs of the present disclosure can result in a significant increase in the in vitro transcription yield of mRNA, the capping rate, the translation efficiency of mRNA, and the amount and duration of protein expression by mRNA in mice, as well as a significant decrease in the decapping rate.
  • C 1 -C 3 refers to a group having any integral number of carbon atoms within the range of 1 to 3 in the main chain, such as 1, 2, or 3 carbon atoms.
  • C 6-15 refers to a group having any integral number of carbon atoms within the range of 6 to 15, such as 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 carbon atoms.
  • alkyl refers to a saturated aliphatic hydrocarbon group having a linear or branched chain; non-limiting examples include methyl, ethyl, propyl, isopropyl, etc.
  • salt refers to a corresponding salt of a modified nucleoside compound (or nucleotide compound) of the present disclosure that can be conveniently or desirably prepared, purified, and/or treated, such as a pharmaceutically acceptable salt. Unless otherwise indicated, references to a specific compound in the present disclosure also include a salt form thereof.
  • cap analog refers to a structure at the 5' end of a mature mRNA formed by post-transcriptional modification in eukaryotes, namely an m7GPPPN structure, also known as a methylguanosine cap.
  • the structure can prevent the degradation of mRNA at the 5' end, help RNA transcripts pass through the selective pores of the nuclear membrane and enter the cytoplasm, enhance translation, and help complete the entire splicing process.
  • pharmaceutically acceptable in the present disclosure means that a compound or composition is chemically and/or toxicologically compatible with the other ingredients making up the preparation and/or with the human or mammal in which it is used to prevent or treat a disease or condition.
  • solvate in the present disclosure refers to a complex formed by combining a compound of formula (I) or a pharmaceutically acceptable salt thereof with a solvent (e.g., ethanol or water). It should be understood that any solvate of a compound of formula I for use in the treatment of a disease or condition may provide different properties (including pharmacokinetic properties), however will result in the compound of formula I upon absorbed into a subject, such that the use of the compound of formula I encompasses the use of any solvate of the compound of formula I respectively.
  • solvent e.g., ethanol or water
  • the compound of formula I or the pharmaceutically acceptable salt thereof may be isolated in the form of a solvate, and therefore any such solvate is included within the scope of the present disclosure.
  • the compound of formula I or the pharmaceutically acceptable salt thereof may exist in an unsolvated form as well as a solvated form with a pharmaceutically acceptable solvent (e.g., water, ethanol).
  • the present disclosure also includes a salt of the compound described herein, especially a pharmaceutically acceptable salt.
  • the compounds of the present disclosure having sufficiently acidic or sufficiently basic functional groups can react with a wide variety of bases or acids to form salts.
  • compounds that are inherently charged e.g., compounds having a quaternary nitrogen
  • may form salts with appropriate counterions e.g., halide ions such as bromide ions, chloride ions, or fluoride ions, especially bromide ions).
  • the pharmaceutically acceptable salt of the present disclosure may be, for example, an acid addition salt of the compound of the present disclosure which is sufficiently basic and bears a nitrogen atom in a chain or ring of the compound of formula (I), for example, an acid addition salt formed with an inorganic acid, such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, phosphoric acid, or nitric acid, or an acid addition salt formed with an organic acid, such as formic acid, acetic acid, acetoacetic acid, pyruvic acid, trifluoroacetic acid, propionic acid, butyric acid, hexanoic acid, heptanoic acid, undecanoic acid, lauric acid, benzoic acid, salicylic acid, 2-(4-hydroxybenzoyl)benzoic acid, camphoric acid, cinnamic acid, cyclopentylpropionic acid, 3-hydroxy-2-naphthoic acid, nicotinic acid, pa
  • an alkali metal salt such as a sodium or potassium salt
  • an alkaline earth metal salt such as a calcium or magnesium salt
  • an ammonium salt e.g., a salt formed with NH 3 or ammonia water
  • a salt formed with an organic base which provides a physiologically acceptable cation, for example, a salt formed with triethylamine, N-methylglucamine, dimethylglucamine, ethylglucamine, lysine, dicyclohexylamine, 1,6-hexanediamine, ethanolamine, glucosamine, sarcosine, serinol, tris(hydroxymethyl)aminomethane, aminopropanediol, 1-amino-2,3,4-butanetriol.
  • basic nitrogen-containing groups can be quaternized with the following reagents: lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides, and iodides; dialkyl sulfates such as dimethyl, diethyl, dibutyl, and diamyl sulfates; long chain halides such as decyl, lauryl, myristyl, and stearyl chlorides, bromides, and iodides; aralkyl halides such as benzyl and phenethyl bromides.
  • lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides, and iodides
  • dialkyl sulfates such as dimethyl, diethyl, dibutyl, and diamyl sulfates
  • long chain halides such as decyl, lauryl, my
  • acid addition salts of the compound of formula (I) of the present disclosure can be prepared by reacting the compound with a suitable inorganic or organic acid by any one of the known methods.
  • base addition salts of the acidic compound of the present disclosure are prepared by reacting the compound with a suitable base by various known methods.
  • the present disclosure includes all possible salts of the compound of formula (I) of the present disclosure, which may be a single salt or any mixture of the salt in any ratio.
  • Stereoisomers include geometric isomers, diastereomers, and enantiomers. Accordingly, the compound of formula (I) of the present disclosure also includes racemic mixtures, single stereoisomers, and optically active mixtures. It should be understood by those skilled in the art that one stereoisomer may have better efficacy and/or lower side effects than other stereoisomers.
  • Single stereoisomers and optically active mixtures can be obtained by methods such as chiral source synthesis, chiral catalysis, and chiral resolution. The racemate can be chirally resolved by chromatographic resolution or chemical resolution.
  • a chiral acid resolution reagent such as chiral tartaric acid and chiral malic acid can be added to form a salt with the compound of the present disclosure, and the physicochemical properties of the product, such as the difference in solubility, can be utilized for separation.
  • compound of the present disclosure may include a compound of formula (I), a solvate thereof, a pharmaceutically acceptable salt thereof, a stereoisomer thereof, or mixtures thereof according to the context.
  • a first aspect of the present disclosure provides a ribose-modified cap analog, or a stereoisomer, a pharmaceutically acceptable salt, or a solvate thereof, wherein the ribose-modified cap analog has a structure of formula (I):
  • X 2 is -O-.
  • X 2 is -O- and R 2 is absent.
  • X 2 is -O- and R 3 is -H.
  • X 2 is -O-
  • R 2 is absent
  • R 3 is -H.
  • X 2 is -CH-.
  • X 2 is -CH- and R 2 is -H, -CH 3 , or -F.
  • X 2 is -CH- and R 3 is -N(CH 3 ) 2 , -C(O)CH 3 , -OCH 3 , -NHC(O)CH 3 , or -F.
  • X 2 is -CH-
  • R 2 is -H, -CH 3 , or -F
  • R 3 is -N(CH 3 ) 2 , -C(O)CH 3 , - OCH 3 , -NHC(O)CH 3 , or -F.
  • X 2 is -CN.
  • X 2 is -CN and R 2 is absent.
  • X 2 is -CN, R 2 is absent, and R 3 is absent.
  • X 2 is
  • X 2 is and R 2 is absent.
  • X 2 is and R 3 is absent.
  • X 2 is R 2 is absent, and R 3 is absent.
  • X 2 is -C(O)-.
  • X 2 is -C(O)- and R 2 is absent.
  • X 2 is -C(O)- and R 3 is -N(CH 2 CH 3 ) 2 or -N(CH 2 CH 2 CH 3 ) 2 .
  • X 2 is -C(O)-, R 2 is absent, and R 3 is -N(CH 2 CH 3 ) 2 or - N(CH 2 CH 2 CH 3 ) 2 .
  • X 3 is -O-.
  • X 3 is -O- and R 7 is -CH 3 or -OCH 3 .
  • X 3 is -CH 2 -.
  • X 3 is -CH 2 - and R 7 is -OCH 3 , -NHC(O)CH 3 , or -F.
  • X 3 is -CFH-.
  • X 3 is -CFH- and R 7 is -F.
  • m is 1.
  • m is 1 and R 5 is -OCH 3 , -F, or -CF 2 H; preferably, m is 1 and R 5 is - OCH 3 .
  • R 6 is -H.
  • the ribose-modified cap analog is YK-CAP-101, YK-CAP-102, YK-CAP-103, YK-CAP-104, YK-CAP-105, YK-CAP-106, YK-CAP-107, YK-CAP-108, YK-CAP-109, YK-CAP-110, YK-CAP-111, YK-CAP-112, YK-CAP-113, YK-CAP-114, YK-CAP-115, YK-CAP-116, YK-CAP-117, YK-CAP-118, or YK-CAP-119 as shown below:
  • a second aspect of the present disclosure provides a use of the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure in the preparation of an in vitro co-transcription mRNA capping reagent.
  • a third aspect of the present disclosure provides an RNA molecule comprising the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure as a cap structure or a cap structure fragment.
  • a fourth aspect of the present disclosure provides a pharmaceutical composition comprising the RNA molecule according to the third aspect of the present disclosure.
  • the pharmaceutical composition further comprises at least one RNA delivery agent.
  • the RNA delivery agent may be, for example, lipid nanoparticles (LNPs).
  • LNPs lipid nanoparticles
  • Lipid nanoparticles are widely used in small molecule drug and nucleic acid delivery.
  • mRNA encapsulated by LNPs can be protected from extracellular ribonucleases and facilitates intracellular delivery of mRNA.
  • lipid nanoparticles please refer to the review " Chemistry of Lipid Nanoparticles for RNA Delivery. Acc Chem Res. 2022 Jan 4; 55(1): 2-12 ".
  • the at least one RNA delivery agent comprises at least one cationic lipid.
  • cationic lipid refers to a lipid that is positively charged at a selected pH value.
  • cationic lipids disclosed in literatures such as WO2023133946A1 , CN115745820A , and " Chemistry of Lipid Nanoparticles for RNA Delivery. Acc Chem Res. 2022 Jan 4; 55(1): 2-12 ".
  • the cationic lipid is selected from one or a combination of at least two of the following compounds:
  • the cationic lipid is selected from one or a combination of at least two of YK-009, YK-401, YK-305, ALC0315, SM102, or DLIN-MC3-DMA:
  • the cationic lipid is YK-009.
  • the at least one RNA delivery agent further comprises at least one neutral lipid.
  • the neutral lipid refers to an auxiliary lipid that is uncharged or exists in a zwitterionic form at a selected pH value.
  • the neutral lipid may regulate the fluidity of nanoparticles into a lipid bilayer structure and improve efficiency by promoting lipid phase transition, and may also affect target organ specificity.
  • the neutral lipid includes one or a combination of at least two of phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, ceramide, sterol, or derivatives thereof.
  • the neutral lipid is selected from one or a combination of at least two of the following: 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (DLPC), 1,2-dimyristoyl-sn-glycero-phosphocholine (DMPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-diundecanoyl-sn-glycero-phosphocholine (DUPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-di-O-octadecenyl-sn-glycero-3-phosphocholine (18:0 Diether PC), 1-oleoyl-2-cholesterylhem
  • the neutral lipid is DOPE and/or DSPC.
  • the at least one RNA delivery agent further comprises a structural lipid.
  • the structural lipid refers to a lipid that enhances the stability of nanoparticles by filling the gaps between lipids.
  • the structural lipid is selected from one or a combination of at least two of the following: cholesterol, nonsterol, sitosterol, ergosterol, campesterol, stigmasterol, brassinosterol, tomatine, ursolic acid, ⁇ -tocopherol, or corticosteroid.
  • the structural lipid is cholesterol.
  • the at least one RNA delivery agent further comprises a polymer-conjugated lipid.
  • the polymer-conjugated lipid mainly refers to a lipid modified with polyethylene glycol (PEG).
  • PEG polyethylene glycol
  • Hydrophilic PEG stabilizes lipid nanoparticles (LNPs), regulates nanoparticle size by limiting lipid fusion, and increases nanoparticle half-life by reducing non-specific interactions with macrophages.
  • the polymer-conjugated lipid is selected from one or a combination of at least two of the following: distearoyl phosphatidylethanolamine polyethylene glycol 2000 (DSPE-PEG2000), dimyristoylglycero-3-methoxypolyethylene glycol 2000 (DMG-PEG2000), or methoxypolyethylene glycol ditetradecylacetamide (ALC-0159).
  • DSPE-PEG2000 distearoyl phosphatidylethanolamine polyethylene glycol 2000
  • DMG-PEG2000 dimyristoylglycero-3-methoxypolyethylene glycol 2000
  • AAC-0159 methoxypolyethylene glycol ditetradecylacetamide
  • the RNA delivery agent comprises a neutral lipid, a structural lipid, and a polymer-conjugated lipid, wherein the molar ratio of the cationic lipid, the neutral lipid, the structural lipid, and the polymer-conjugated lipid is (25 to 75):(5 to 25):(15 to 65):(0.5 to 10), such as (35 to 49):(7.5 to 15):(35 to 55):(1 to 5).
  • the pharmaceutical composition further comprises one or at least two cell-penetrating peptides.
  • the present disclosure provides a kit comprising: (1) the ribose-modified cap analog, or the stereoisomer, the pharmaceutically acceptable salt, or the solvate thereof according to the first aspect of the present disclosure; and (2) a nucleotide triphosphate molecule and an RNA polymerase.
  • the kit further comprises one or a combination of at least two of an RNAase inhibitor, an inorganic pyrophosphatase, Mg 2+ , a crowding agent, or a buffer.
  • Step 1 Synthesis of INT-I-PM1
  • INT-I-PM1 (60.0 g, 0.31 mol) was dissolved in pyridine (200 mL), then N,N- diisopropylethylamine (120.2 g, 0.93 mol) was added thereto, and the mixture was cooled to 0°C.
  • Diphenylcarbamoyl chloride (86.2 g, 0.37 mol) was dissolved in pyridine (100 mL), and the mixture was slowly added dropwise to the above reaction system in an ice bath. After the dropwise addition was completed, the ice bath was removed. The reaction mixture was naturally warmed to room temperature, and stirred and reacted for 3 hours. LCMS monitored that there was no starting material remaining and the reaction was complete.
  • the reaction system was quenched with 100 mL of water, and evaporated to dryness by rotary evaporation under reduced pressure. The residue was added with 800 mL of a mixed solvent of ethanol and water (1:1, v/v), and heated to reflux for 2 hours. The heating was stopped, and the reaction mixture was naturally cooled to room temperature to precipitate a large amount of solid, which was filtered. The filter cake was washed with ethanol to obtain INT-I (59.7 g, 0.15 mol, 49.6%). C 20 H 16 N 6 O 3 , MS (ES): m/z (M+H + ) 389.1.
  • pA(2'-OMe)mpG ⁇ TEA (300.1 mg, 0.37 mmol), imidazole (347.2 mg, 5.10 mmol), dithiodipyridine (1123.6 mg, 5.10 mmol), and triethylamine (516.1 mg, 5.10 mmol) were dissolved in 2.0 mL of ultra-dry N , N -dimethylformamide, then triphenylphosphine (1337.7 mg, 5.10 mmol) was added thereto under nitrogen atmosphere, and the mixture was reacted at 25°C for 4 hours.
  • Step 1 Synthesis of YK-CAP-101-PM1
  • 2-amino-6-chloropurine (6.29 g, 37.09 mmol), toluene (50 mL), and BSA(15.09 g, 74.19 mmol).
  • the system was heated to 80°C, stirred until clarified, and naturally cooled to room temperature.
  • ⁇ -Pentaacetylglucose (10.00 g, 25.62 mmol) was dissolved in 20 mL of toluene, and the mixture was added to the system, which was stirred and reacted at room temperature for 5 minutes.
  • TMSOTf (8.24 g, 37.09 mmol) was added thereto at room temperature, and the system was heated to 110°C and reacted at 110°C for 3 hours. After the reaction was completed, the system was naturally cooled to room temperature, added with 100 mL of saturated sodium bicarbonate solution and 100 mL of ethyl acetate, stirred for 5 minutes, and filtered through diatomite. The phases were separated, and the aqueous phase was extracted with 100 mL of ethyl acetate. The ethyl acetate phases were combined, washed with 100 mL of saturated brine, dried over anhydrous sodium sulfate, and filtered.
  • Step 2 Synthesis of YK-CAP-101-PM2
  • Step 3 Synthesis of YK-CAP-101-PM3
  • YK-CAP-101-PM2 300 mg, 0.96 mmol was dissolved in 3 mL of trimethyl phosphate. The mixture was cooled to 0°C under nitrogen atmosphere, then phosphorus oxychloride (450 mg, 2.93 mmol) was slowly added dropwise thereto, and the mixture was stirred and reacted at 0°C for about 3 hours. After the reaction was completed, the mixture was added with 5 mL of water, warmed to room temperature, stirred for about 1.5 hours, then washed with dichloromethane (10 mL), and left for phase separation. The upper aqueous phase was collected and concentrated under reduced pressure.
  • the concentrated mixture was diluted with water to 180 mL, and purified by gel column chromatography (eluted with water and 1.5 M TEAB at a ratio of 10:1).
  • the target product peak was collected, concentrated, and lyophilized to obtain YK-CAP-101-PM3 (triethylamine salt, 340 mg, 0.69 mmol, 71.6%) as a white solid.
  • Step 4 Synthesis of YK-CAP-101-PM4
  • YK-CAP-101-PM3 (340 mg, 0.69 mmol), imidazole (706 mg, 10.38 mmol), 2,2'-dithiodipyridine (2287 mg, 10.38 mmol), triethylamine (1050 mg, 10.38 mmol), and triphenylphosphine (2723 mg, 10.38 mmol) were dissolved in 4 mL of dry N,N- dimethylformamide. The mixture was stirred and reacted at room temperature for about 4 hours under nitrogen atmosphere.
  • Step 5 Synthesis of YK-CAP-101-PM5
  • YK-CAP-101-PM4 (309 mg, 0.66 mmol) and TEAP (458 mg, 2.30 mmol) were dissolved in dry N,N- dimethylformamide (5 mL), then zinc chloride (215 mg, 1.58 mmol) was added thereto, and the mixture was stirred and reacted at room temperature for about 21 hours under nitrogen atmosphere. After the reaction was completed, the system was added with MTBE (10 mL), washed with ultrasonic stirring, allowed to stand, and the supernatant was poured out. The procedure was repeated once. The bottom substance was collected and concentrated under reduced pressure.
  • Step 6 Synthesis of YK-CAP-101-PM6
  • YK-CAP-101-PM5 (320 mg, 0.56 mmol) and iodomethane (960 mg, 6.76 mmol) were dissolved in dry N , N -dimethylformamide (4 mL), and the mixture was stirred and reacted in an oil bath at 37°C for about 23 hours. After the reaction was completed, the system was dissolved in water (5 mL) until clarified, washed with EA (25 mL), and the phases were separated. The lower aqueous phase was collected and concentrated under reduced pressure. The residue was dissolved in water (50 mL) until clarified, and purified by gel column chromatography (eluted with water and 1.5 M TEAB at a ratio of 10:1).
  • the target product peak was collected, concentrated, lyophilized, and further desalted by preparative high performance liquid chromatography (50 mM TEAB and methanol mobile phase system) to obtain YK-CAP-101-PM6 (triethylamine salt, 95 mg, 0.16 mmol, 28.8%) as a white solid.
  • Step 7 Synthesis of YK-CAP-101
  • YK-CAP-101-PM6 95 mg, 0.16 mmol
  • INT-II 219 mg, 0.27 mmol
  • dry dimethyl sulfoxide 1.2 mL
  • zinc chloride 518 mg, 3.80 mmol
  • the mixture was stirred and reacted in an oil bath at 37°C for about 3 days under nitrogen atmosphere.
  • the mixture was dissolved in 0.25 M EDTA solution until clarified, then added with 1.5 M TEAB to adjust the pH to 6 to 7, and purified by gel column chromatography (eluted with water and 1.5 M TEAB at a ratio of 10:1).
  • Step 1 Synthesis of YK-CAP-102-PM1
  • YK-CAP-102-PM1 (8.79 g, 17.62 mmol) was used as starting material to obtain YK-CAP-102-PM2 (2.37 g, 6.69 mmol, 37.96%).
  • YK-CAP-102-PM2 (500 mg, 1.41 mmol) was used as starting material to obtain YK-CAP-102-PM3 (triethylamine salt, 702 mg, 1.31 mmol, 93.0%).
  • Step 4 Synthesis of intermediate YK-CAP-102-PM4
  • YK-CAP-102-PM3 (702 mg, 1.31 mmol) was used as starting material to obtain YK-CAP-102-PM4 (sodium salt, 521 mg, 1.03 mmol, 78.5%).
  • Step 5 Synthesis of intermediate YK-CAP-102-PM5
  • YK-CAP-102-PM4 521 mg, 1.03 mmol was used as starting material to obtain YK-CAP-102-PM5 (triethylamine salt, 338 mg, 0.55 mmol, 53.3%).
  • Step 6 Synthesis of intermediate YK-CAP-102-PM6
  • YK-CAP-102-PM5 (338 mg, 0.55 mmol) was used as starting material to obtain YK-CAP-102-PM6 (triethylamine salt, 158 mg, 0.25 mmol, 45.6%).
  • Step 7 Synthesis of YK-CAP-102
  • YK-CAP-102-PM6 158 mg, 0.25 mmol was used as starting material to obtain YK-CAP-102 (23.9 mg, 18.85 ⁇ mol, 7.5%).
  • Step 1 Synthesis of YK-CAP-103-PM1
  • Step 2 Synthesis of YK-CAP-103-PM2
  • Step 3 Synthesis of YK-CAP-103-PM3
  • YK-CAP-103-PM2 (20.0 g, 41.27 mmol) was dissolved in a single-necked flask containing 150 mL of DCM. The mixture was cooled to -78°C, and a solution of diisobutylaluminum hydride in toluene (1.5 M, 63.2 mL, 94.8 mmol) was slowly added dropwise thereto. After the dropwise addition was completed, the mixture was warmed to room temperature, and stirred and reacted overnight. After the reaction was completed, the mixture was slowly added with 65 mL of methanol in an ice bath, followed by the generation of a white flocculent solid.
  • Step 4 Synthesis of YK-CAP-103-PM4
  • Step 5 Synthesis of YK-CAP-103-PM5
  • YK-CAP-103-PM4 (17.00 g, 27.83 mmol) was dissolved in a single-necked flask containing tetrahydrofuran (150 mL), and a solution of potassium tert -butoxide in tetrahydrofuran (1 M, 61.0 mL, 61.0 mmol) was slowly added dropwise thereto at -40°C. After the dropwise addition was completed, the mixture was stirred and reacted at room temperature for 3 hours, diluted with ethyl acetate (200 mL), and washed with saturated brine (150 mL ⁇ 2). The organic phase was dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under vacuum. The residue was purified by silica gel chromatography (0 to 11% ethyl acetate/ n- hexane) to obtain YK-CAP-103-PM5 (3.29 g, 7.50 mmol, 27.0%).
  • Step 6 Synthesis of YK-CAP-103-PM6
  • YK-CAP-103-PM5 (3.29 g, 7.50 mmol) was dissolved in a mixed solution of tetrahydrofuran (25 mL) and water (5 mL). Potassium osmate dihydrate (140 mg, 0.38 mmol) and N -methylmorpholine- N -oxide (1.05 g, 9.00 mmol) were sequentially added to the above mixed solution. The mixture was heated to 40°C, and stirred and reacted at 40°C for 6 hours. After the reaction was completed, the mixture was diluted with ethyl acetate (100 mL) and washed with saturated sodium sulfite aqueous solution (80 mL ⁇ 2). The organic phase was dried over anhydrous sodium sulfate, filtered, and the solvent was removed under vacuum to obtain YK-CAP-103-PM6 (3.48 g, 7.36 mmol, 98.2%).
  • Step 7 Synthesis of YK-CAP-103-PM7
  • YK-CAP-103-PM6 (3.48 g, 7.36 mmol) was dissolved in a mixed solution of tetrahydrofuran (25 mL) and water (5 mL). Potassium periodate (2.54 g, 11.04 mmol) was sequentially added to the above mixed solution. The mixture was heated to 40°C, and stirred and reacted at 40°C for 6 hours. After the reaction was completed, the mixture was diluted with ethyl acetate (100 mL) and washed with saturated sodium sulfite aqueous solution (50 mL ⁇ 2). The organic phase was dried over anhydrous sodium sulfate, filtered, and the solvent was removed under vacuum to obtain YK-CAP-103-PM7 (3.14 g, 7.13 mmol, 96.9%).
  • Step 8 Synthesis of YK-CAP-103-PM8
  • YK-CAP-103-PM7 (5.19 g, 11.78 mmol) was dissolved in a single-necked flask containing methanol (100 mL), then sodium borohydride (0.54 g, 13.18 mmol) was added thereto in batches in an ice bath, and the mixture was stirred and reacted at room temperature for 3 hours. After the reaction was completed, the mixture was diluted with water (150 mL) and extracted with ethyl acetate (150 mL ⁇ 2). The organic phases were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The residue was purified by silica gel chromatography (0 to 13% ethyl acetate/n-hexane) to obtain YK-CAP-103-PM8 (3.30 g, 7.46 mmol, 63.3%).
  • Step 9 Synthesis of YK-CAP-103-PM9
  • YK-CAP-103-PM8 (3.30 g, 7.46 mmol) was used as starting material to obtain YK-CAP-103-PM9 (3.42 g, 5.73 mmol, 76.8%).
  • Step 10 Synthesis of YK-CAP-103-PM10
  • Step 11 Synthesis of YK-CAP-103-PM11
  • YK-CAP-103-PM10 (1.90 g, 3.20 mmol) was dissolved in tetrahydrofuran (40 mL), then 1 M HCl (80 mL) was added thereto, and the mixture was stirred and reacted at 90°C for 7 hours. After the reaction was completed, the solvent was removed under reduced pressure, and the residue was purified by preparative high-pressure liquid chromatography to obtain compound YK-CAP-103-PM11 (587 mg. 1.97 mmol, 61.7%) as a white solid. C 11 H 15 N 5 O 5 , MS (ES): m/z (M+H + ) 298.2.
  • YK-CAP-103-PM11 1 H NMR (400 MHz, DMSO-d 6 ) ⁇ 10.57 (s, 1H), 7.68 (s, 1H), 6.46 (s, 2H), 5.00 - 4.53 (m, 2H), 4.17 - 4.09 (m, 1H), 4.00 - 3.87 (m, 2H), 3.77 - 3.61 (m, 3H), 3.46 - 3.32 (m, 3H).
  • Step 12 Synthesis of YK-CAP-103-PM12
  • YK-CAP-101-PM3 YK-CAP-103-PM11 (300 mg, 1.01 mmol) was used as starting material to obtain YK-CAP-103-PM12 (triethylamine salt, 350 mg, 0.73 mmol, 72.5%).
  • Step 13 Synthesis of YK-CAP-103-PM13
  • YK-CAP-103-PM12 350 mg, 0.73 mmol was used as starting material to obtain YK-CAP-103-PM13 (sodium salt, 320 mg, 0.71 mmol, 97.4%).
  • Step 14 Synthesis of YK-CAP-103-PM14
  • YK-CAP-103-PM13 (320 mg, 0.71 mmol) was used as starting material to obtain YK-CAP-103-PM14 (triethylamine salt, 250 mg, 0.45 mmol, 62.9%).
  • Step 15 Synthesis of YK-CAP-103-PM15
  • YK-CAP-101-PM6 YK-CAP-103-PM14 (250 mg, 0.45 mmol) was used as starting material to obtain YK-CAP-103-PM15 (triethylamine salt, 90 mg, 0.16 mmol, 34.9%).
  • Step 16 Synthesis of YK-CAP-103
  • YK-CAP-103-PM15 (90 mg, 0.16 mmol) was used as starting material to obtain YK-CAP-103 (29 mg, 23.95 ⁇ mol, 15.0%).
  • Step 1 Synthesis of YK-CAP-104-PM1
  • 1,2-O-isopropyl-A-D-ribofuranose (20.0 g, 0.11 mol) was dissolved in dichloromethane.
  • Imidazole (11.6 g, 0.17 mol) and TBDPSCl (33.0 g, 0.12 mol) were added to the above system.
  • the mixture was stirred and reacted at room temperature for 15 hours.
  • the reaction mixture was added with saturated sodium bicarbonate solution and extracted with dichloromethane.
  • the dichloromethane phase was then washed once with saturated brine, dried over anhydrous sodium sulfate, filtered, and the filtrate was evaporated to dryness by rotary evaporation under reduced pressure.
  • Step 2 Synthesis of YK-CAP-104-PM2
  • YK-CAP-104-PM1 (33.5 g, 78.16 mmol) was dissolved in acetonitrile, and 2-iodoxybenzoic acid (28.5 g, 101.8 mmol) was added thereto. The mixture was heated to 90°C, and stirred and reacted for 5 hours. The reaction mixture was filtered, and the filtrate was evaporated to dryness by rotary evaporation under reduced pressure to obtain YK-CAP-104-PM2 (32.7 g, 76.66 mmol, 98.1%).
  • Step 3 Synthesis of YK-CAP-104-PM3
  • the above reaction system was re-cooled to -78°C, and a solution of YK-CAP-104-PM2 (48.0 g, 112.52 mmol) in tetrahydrofuran (100 mL) was slowly added dropwise thereto. After the dropwise addition was completed, the reaction system was warmed to room temperature, and stirred and reacted overnight. The reaction system was quenched with saturated ammonium chloride solution (200 mL) and extracted with ethyl acetate (300 mL ⁇ 3). The organic phases were combined, washed with saturated NaCl aqueous solution, dried over anhydrous sodium sulfate, filtered, and evaporated to dryness by rotary evaporation.
  • Step 4 Synthesis of YK-CAP-104-PM4
  • YK-CAP-104-PM3 (34.0 g, 80.07 mmol) was dissolved in tetrahydrofuran (200 mL), then tetrabutylammonium fluoride (52.0 g, 198.9 mmol) was added thereto, and the mixture was stirred and reacted at room temperature for 1 hour.
  • the reaction system was added with saturated ammonium chloride aqueous solution and extracted with ethyl acetate (200 mL ⁇ 3). The organic phases were combined, washed with saturated NaCl aqueous solution, dried over anhydrous sodium sulfate, filtered, and evaporated to dryness by rotary evaporation. The residue was purified by silica gel chromatography (0 to 60% ethyl acetate/n-hexane) to obtain YK-CAP-104-PM4 (13.5 g, 72.50 mmol, 90.5%).
  • Step 5 Synthesis of YK-CAP-104-PM5
  • YK-CAP-104-PM4 (13.5 g, 72.50 mmol) was dissolved in dichloromethane (150 mL), and triethylamine (22.0 g, 217.4 mmol) was added thereto.
  • the reaction system was cooled to 0°C, and benzoyl chloride (11.2 g, 79.7 mmol) was slowly added dropwise thereto.
  • the reaction system was naturally warmed to room temperature, and stirred and reacted for 1 hour. After the reaction was completed, the reaction system was quenched with saturated sodium bicarbonate aqueous solution (100 mL) and extracted with dichloromethane (100 mL ⁇ 3).
  • Step 6 Synthesis of YK-CAP-104-PM6
  • YK-CAP-104-PM5 (18.6 g, 64.07 mmol) was dissolved in a mixed solvent of tetrahydrofuran (160 mL) and water (40 mL).
  • N-methylmorpholine oxide (11.3 g, 96.5 mmol) and potassium osmate dihydrate (2.0 g, 6.4 mmol) were sequentially weighed and added thereto. The mixture was stirred and reacted at room temperature overnight. After the reaction was completed, the reaction system was quenched with saturated sodium sulfite aqueous solution and extracted with ethyl acetate (200 mL ⁇ 3).
  • Step 7 Synthesis of YK-CAP-104-PM7
  • YK-CAP-104-PM6 (17.7 g, 54.57 mmol) as starting material was dissolved in acetonitrile (140 mL) and pyridine (140 mL). Imidazole (11.1 g, 163.8 mmol), triphenylphosphine (21.5 g, 81.9 mmol), and carbon tetrabromide (27.1 g, 81.9 mmol) were sequentially weighed and added thereto. The above reaction system was heated to 70°C under nitrogen atmosphere, and stirred and reacted for 6 hours. Thin-layer chromatography (TLC) monitored that the reaction was complete. The reaction mixture was evaporated to dryness by rotary evaporation under vacuum. The residue was purified by silica gel chromatography (0 to 50% ethyl acetate/n-hexane) to obtain YK-CAP-104-PM7 (13.9 g, 35.90 mmol, 65.8%).
  • Step 8 Synthesis of YK-CAP-104-PM8
  • YK-CAP-104-PM7 (13.4 g, 34.60 mmol) was dissolved in acetonitrile (120 mL). Potassium carbonate (14.4 g, 104.2 mmol) and dimethylamine hydrochloride (3.4 g, 41.1 mmol) were sequentially added thereto. The above system was heated to 70°C and reacted for 16 hours. TLC monitored that the reaction was complete. The system was cooled to room temperature, filtered, and the organic phase was evaporated to dryness by rotary evaporation under reduced pressure.
  • Step 9 Synthesis of YK-CAP-104-PM9
  • YK-CAP-104-PM8 (8.5 g, 24.18 mmol) was dissolved in dichloromethane (160 mL). The mixture was cooled to -40°C, and a solution of diethylaminosulfur trifluoride (4.7 g, 29.0 mmol) in dichloromethane (10 mL) was slowly added dropwise thereto. After the dropwise addition was completed, the mixture was slowly warmed to 0°C, and stirred and reacted for 4 hours. TLC monitored that the reaction was complete. The reaction system was quenched with saturated sodium bicarbonate aqueous solution (100 mL) and extracted with dichloromethane (100 mL ⁇ 3).
  • Step 10 Synthesis of YK-CAP-104-PM10
  • YK-CAP-104-PM9 (4.7 g, 13.30 mmol) was dissolved in acetic acid (100 mL), then acetic anhydride (16.4 g, 160.6 mmol) was added thereto, and concentrated sulfuric acid (4.7 g, 26.8 mmol) was slowly added dropwise thereto. After the dropwise addition was completed, the mixture was slowly heated to 40°C, and stirred and reacted for 16 hours. LCMS monitored that the reaction was complete. The reaction system was quenched with saturated sodium bicarbonate aqueous solution to adjust the pH to neutrality, and extracted with ethyl acetate (200 mL ⁇ 3).
  • Step 11 Synthesis of YK-CAP-104-PM11
  • Step 12 Synthesis of YK-CAP-104-PM12
  • YK-CAP-104-PM11 (2.7 g, 3.72 mmol) was dissolved in a mixed solvent of NH 3 /MeOH (20 mL) and water (4 mL). The above reaction system was heated to 50°C, and stirred and reacted for 10 hours. LCMS monitored that the reaction was complete. The reaction mixture was evaporated to dryness by rotary evaporation to obtain 2.5 g of a crude product, which was purified by preparative high-pressure liquid chromatography to obtain YK-CAP-104-PM12 (560 mg, 1.64 mmol, 44.0%). C 13 H 19 FN 6 O 4 , MS (ES): m/z (M+H + ) 343.1.
  • Step 13 Synthesis of YK-CAP-104-PM13
  • Step 14 Synthesis of YK-CAP-104-PM14
  • Step 15 Synthesis of YK-CAP-104-PM15
  • YK-CAP-104-PM14 (414 mg, 0.84 mmol) was used as starting material to obtain YK-CAP-104-PM15 (triethylamine salt, 300 mg, 0.50 mmol, 59.2%).
  • YK-CAP-104-PM15 triethylamine salt, 300 mg, 0.50 mmol, 59.2%.
  • Step 16 Synthesis of YK-CAP-104-PM16
  • YK-CAP-101-PM6 YK-CAP-104-PM15 (300 mg, 0.50 mmol) was used as starting material to obtain YK-CAP-104-PM16 (triethylamine salt, 124 mg, 0.20 mmol, 40.2%).
  • YK-CAP-104-PM16 triethylamine salt, 124 mg, 0.20 mmol, 40.2%.
  • Step 17 Synthesis of YK-CAP-104
  • YK-CAP-104-PM16 (124 mg, 0.20 mmol) was used as starting material to obtain YK-CAP-104 (ammonium salt, 21 mg, 16.72 ⁇ mol, 8.4%).
  • YK-CAP-104 (ammonium salt, 21 mg, 16.72 ⁇ mol, 8.4%).
  • Step 1 Synthesis of YK-CAP-105-PM1
  • YK-CAP-104-PM4 (5.76 g, 30.93 mmol) was dissolved in acetic acid (8 mL), then acetic anhydride (31.6 g, 310 mmol) and sulfuric acid (500 ⁇ L) were added thereto, and the mixture was stirred and reacted at room temperature for 4 hours. After the reaction was completed, the reaction mixture was added with NaHCO 3 aqueous solution to adjust the pH to weak acidity, and extracted twice with ethyl acetate.
  • Step 2 Synthesis of YK-CAP-105-PM2
  • Trimethylsilyl trifluoromethanesulfonate (5.31 g, 23.9 mmol) was then added thereto, and the mixture was reacted at 70°C for 2 hours. TLC monitored that the reaction was complete. After filtration, the filtrate was evaporated to dryness by rotary evaporation under reduced pressure. The residue was purified by silica gel column chromatography (0 to 80% ethyl acetate/n-hexane) to obtain YK-CAP-105-PM2 (10.00 g, 16.65 mmol, 76.8%).
  • Step 3 Synthesis of YK-CAP-105-PM3
  • YK-CAP-105-PM2 (8.00 g, 13.32 mmol) and [ N , N '-(1,1,2,2-tetramethylethane)bis(3,5-di-tert-butylsalicylideneimine)]cobalt(II) (403 mg, 0.67 mmol) were dissolved in 1,4-dioxane (30 mL), then benzenesulfonyl cyanide (72.30 g, 400 mmol) was added thereto, and the mixture was stirred and reacted at room temperature for 5 minutes. Phenylsilane (1.73 g, 16 mmol) was dissolved in anhydrous ethanol (60 mL) and added to the above mixture.
  • Step 4 Synthesis of YK-CAP-105-PM4
  • YK-CAP-105-PM3 (4.50 g, 7.17 mmol) was dissolved in 7 M ammonia/methanol (50 mL), and the mixture was stirred and reacted at 50°C for 4 hours. After the reaction was completed, the reaction mixture was evaporated to dryness by rotary evaporation to remove the solvent, and the residue was purified by preparative high-pressure liquid chromatography to obtain YK-CAP-105-PM4 (800 mg. 2.61 mmol, 36.4%). C 12 H 14 N 6 O 4 , MS (ES): m/z (M+H + ) 307.11.
  • Step 5 Synthesis of YK-CAP-105-PM5
  • YK-CAP-101-PM3 YK-CAP-105-PM4 (800 mg, 2.61 mmol) was used as starting material to obtain YK-CAP-105-PM5 (triethylamine salt, 994 mg, 2.04 mmol, 78.1%).
  • Step 6 Synthesis of YK-CAP-105-PM6
  • YK-CAP-105-PM5 (994 mg, 2.04 mmol) was used as starting material to obtain YK-CAP-105-PM6 (sodium salt, 661 mg, 1.44 mmol, 70.7%).
  • Step 7 Synthesis of YK-CAP-105-PM7
  • YK-CAP-105-PM6 (661 mg, 1.44 mmol) was used as starting material to obtain YK-CAP-105-PM7 (triethylamine salt, 612 mg, 1.08 mmol, 74.8%).
  • YK-CAP-105-PM7 triethylamine salt, 612 mg, 1.08 mmol, 74.8%.
  • Step 8 Synthesis of YK-CAP-105-PM8
  • Step 9 Synthesis of YK-CAP-105
  • YK-CAP-105-PM8 (325 mg, 0.56 mmol) was used as starting material to obtain YK-CAP-105 (16 mg, 13.12 ⁇ mol, 2.3%).
  • Step 1 Synthesis of YK-CAP-106-PM1
  • Step 2 Synthesis of YK-CAP-106-PM2
  • Step 3 Synthesis of YK-CAP-106-PM3
  • Step 4 Synthesis of YK-CAP-106-PM4
  • YK-CAP-106-PM4 1 H NMR (400 MHz, MeOD) ⁇ 7.99 (s, 1H), 5.84 (d, 1H), 4.77 (s, 1H), 4.66 (d, 1H), 3.93 - 3.82 (m, 2H), 2.06 (s, 3H), 1.63 (s, 3H).
  • Step 5 Synthesis of YK-CAP-106-PM5
  • YK-CAP-101-PM3 YK-CAP-106-PM4 (500 mg, 1.48 mmol) was used as starting material to obtain YK-CAP-106-PM5 (triethylamine salt, 480 mg, 0.92 mmol, 62.4%).
  • YK-CAP-106-PM5 triethylamine salt, 480 mg, 0.92 mmol, 62.4%.
  • Step 6 Synthesis of YK-CAP-106-PM6
  • YK-CAP-106-PM5 (480 mg, 0.92 mmol) was used as starting material to obtain YK-CAP-106-PM6 (sodium salt, 410 mg, 0.84 mmol, 90.9%).
  • Step 7 Synthesis of YK-CAP-106-PM7
  • YK-CAP-106-PM6 (410 mg, 0.84 mmol) was used as starting material to obtain YK-CAP-106-PM7 (triethylamine salt, 331 mg, 0.55 mmol, 65.7%).
  • Step 8 Synthesis of YK-CAP-106-PM8
  • YK-CAP-101-PM6 YK-CAP-106-PM7 (331 mg, 0.55 mmol) was used as starting material to obtain YK-CAP-106-PM8 (triethylamine salt, 150 mg, 0.24 mmol, 44.5%).
  • Step 9 Synthesis of YK-CAP-106
  • YK-CAP-106-PM8 150 mg, 0.24 mmol was used as starting material to obtain YK-CAP-106 (32 mg, 25.56 ⁇ mol, 10.6%).
  • Step 1 Synthesis of YK-CAP-107-PM1
  • Step 2 Synthesis of YK-CAP-107-PM2
  • YK-CAP-107-PM1 (34.20 g, 77.27 mmol) was dissolved in acetonitrile, and 2-iodoxybenzoic acid (28.10 g, 100.5 mmol) was added to the above system. The mixture was heated to 90°C, and stirred and reacted for 5 hours. The reaction mixture was cooled to room temperature, filtered, and the filtrate was evaporated to dryness by rotary evaporation to obtain crude product YK-CAP-107-PM2 (34.50 g) as a light yellow oily liquid, which was directly used in the next step without purification.
  • Step 3 Synthesis of YK-CAP-107-PM3
  • Step 4 Synthesis of YK-CAP-107-PM4
  • YK-CAP-107-PM3 (16.80 g, 36.79 mmol) was dissolved in THF, and the mixture was cooled to 0°C under nitrogen atmosphere. A solution of sodium tert-butoxide (11.20 g, 116.7 mmol) in THF was slowly added to the above system. The mixture was stirred and reacted at room temperature for 1.5 hours, and iodomethane (27.60 g, 194.5 mmol) was slowly added dropwise to the above system. After the dropwise addition was completed, the mixture was stirred and reacted for another 3 hours. TLC detected that the reaction was complete. The reaction mixture was quenched with saturated ammonium chloride solution and extracted with ethyl acetate.
  • Step 5 Synthesis of YK-CAP-107-PM5
  • YK-CAP-107-PM4 (13.16 g, 27.96 mmol) was dissolved in glacial acetic acid (130 mL). Acetic anhydride (17.13 g, 167.8 mmol) and concentrated sulfuric acid (0.52 mL) were sequentially added to the above system. The mixture was stirred and reacted at room temperature for 4 hours. The reaction system was diluted with ethyl acetate, washed once with water, and then washed three times with saturated sodium bicarbonate solution. The organic phase was dried, and evaporated to dryness by rotary evaporation to obtain YK-CAP-107-PM5 (11.38 g) as a yellow oily liquid, which was directly used in the next reaction step.
  • Step 6 Synthesis of YK-CAP-107-PM6
  • 2-Acetamido-9 H -purin-6-yldiphenylcarbamate (6.30 g, 16.22 mmol) was dissolved in 1,2-dichloroethane (100 mL), and N,O-bis(trimethylsilyl)acetamide (6.60 g, 32.4 mmol) was added thereto. The mixture was heated to 80°C, stirred and reacted for 1.5 hours, and subjected to rotary evaporation to remove the reaction solvent.
  • a solution of YK-CAP-107-PM5 (6.00 g, calculated as 18.85 mmol) in toluene (100 mL) and TMSOTf (3.6 g, 16.2 mmol) were then sequentially added thereto at room temperature.
  • Step 7 Synthesis of YK-CAP-107-PM7
  • YK-CAP-107-PM6 (3.20 g, 4.95 mmol) was dissolved in 7 M ammonia/methanol solution and water (5:1, 24 mL). The mixture was heated to 50°C, and stirred and reacted for 6 hours. The reaction mixture was evaporated to dryness by rotary evaporation, slurried twice with EA, and subjected to suction filtration to collect the filter cake to obtain YK-CAP-107-PM7 (1.38 g, 4.24 mmol, 85.7%) as a white solid.
  • Step 8 Synthesis of YK-CAP-107-PM8
  • Step 9 Synthesis of YK-CAP-107-PM9
  • YK-CAP-107-PM8 (644 mg, 1.27 mmol) was used as starting material to obtain YK-CAP-107-PM9 (sodium salt, 549 mg, 1.15 mmol, 90.6%).
  • YK-CAP-107-PM9 sodium salt, 549 mg, 1.15 mmol, 90.6%.
  • Step 10 Synthesis of YK-CAP-107-PM10
  • YK-CAP-107-PM9 549 mg, 1.15 mmol was used as starting material to obtain YK-CAP-107-PM10 (triethylamine salt, 531 mg, 0.91 mmol, 78.7%).
  • Step 11 Synthesis of YK-CAP-107-PM11
  • YK-CAP-107-PM10 531 mg, 0.91 mmol was used as starting material to obtain YK-CAP-107-PM11 (triethylamine salt, 163 mg, 0.27 mmol, 29.8%).
  • Step 12 Synthesis of YK-CAP-107
  • YK-CAP-107-PM11 (163 mg, 0.27 mmol) was used as starting material to obtain YK-CAP-107 (35 mg, 28.25 ⁇ mol, 10.5%).
  • Step 1 Synthesis of YK-CAP-108-PM1
  • YK-CAP-107-PM3 (17.5 g, 38.32 mmol) was dissolved in 300 mL of THF, and triphenylphosphine (12.1 g, 46.00 mmol) and phthalimide (6.76 g, 46.00 mmol) were added thereto.
  • the system was cooled to 0°C under nitrogen atmosphere, and a solution of DEAD (9.3 g, 53.40 mmol) in THF (30 mL) was added dropwise thereto. After the dropwise addition was completed, the mixture was reacted for 3 hours. TLC monitored that the reaction was complete (DCM). After the reaction was completed, the system was added dropwise with 30 mL of purified water at 0°C to quench the reaction.
  • reaction mixture was extracted with EA (200 mL ⁇ 3).
  • the organic phase was sequentially washed with saturated brine (400 mL), dried over anhydrous sodium sulfate, and evaporated to dryness by rotary evaporation.
  • Step 2 Synthesis of YK-CAP-108-PM2
  • Step 3 Synthesis of YK-CAP-108-PM3
  • the system was warmed to room temperature, added with 200 mL of saturated sodium bicarbonate solution to quench the reaction, and the phases were separated.
  • the aqueous phase was extracted with DCM (200 mL ⁇ 2).
  • the organic phase was sequentially washed with saturated brine (300 mL), dried over anhydrous sodium sulfate, and evaporated to dryness by rotary evaporation.
  • Step 4 Synthesis of YK-CAP-108-PM4
  • Step 5 Synthesis of YK-CAP-108-PM5
  • YK-CAP-108-PM4 (4.5 g, 17.35 mmol) was dissolved in 15 mL of acetic acid, then acetic anhydride (35.4 g, 34.71 mmol) and p -toluenesulfonic acid (1.5 g, 8.67 mmol) were added thereto, and the system was heated to 50°C. The reaction was monitored by TLC. After the reaction was completed, the system was cooled to room temperature, added with 100 mL of purified water and 100 mL of EA, stirred for 10 minutes, and the phases were separated. The aqueous phase was extracted with EA (100 mL ⁇ 2).
  • Step 6 Synthesis of YK-CAP-108-PM6
  • Step 7 Synthesis of YK-CAP-108-PM7
  • YK-CAP-108-PM6 (3.8 g, 5.64 mmol) was dissolved in a mixed solvent of 4 M NH 3 /MeOH (40 mL) and water (4 mL), and the mixture was stirred and reacted at room temperature overnight. LCMS monitored that the reaction was complete. The reaction mixture was evaporated to dryness by rotary evaporation, and slurried with EA (100 mL ⁇ 2) at room temperature to obtain YK-CAP-108-PM7 (1.92 g, 5.45 mmol, 96.6%) as an off-white solid. C 14 H 20 N 6 O 5 , MS (ES): m/z (M+H + ) 353.1.
  • Step 8 Synthesis of YK-CAP-108-PM8
  • Step 9 Synthesis of YK-CAP-108-PM9
  • YK-CAP-108-PM8 (1.11 g, 2.08 mmol) was used as starting material to obtain YK-CAP-108-PM9 (sodium salt, 778 mg, 1.54 mmol, 74.2%).
  • Step 10 Synthesis of YK-CAP-108-PM10
  • YK-CAP-108-PM9 (778 mg, 1.54 mmol) was used as starting material to obtain YK-CAP-108-PM10 (triethylamine salt, 512 mg, 0.83 mmol, 54.2%).
  • Step 11 Synthesis of YK-CAP-108-PM11
  • YK-CAP-101-PM6 YK-CAP-108-PM10 (512 mg, 0.83 mmol) was used as starting material to obtain YK-CAP-108-PM11 (triethylamine salt, 117 mg, 0.19 mmol, 22.5%).
  • Step 12 Synthesis of YK-CAP-108
  • YK-CAP-108-PM11 (117 mg, 0.19 mmol) was used as starting material to obtain YK-CAP-108 (18 mg, 14.22 ⁇ mol, 7.5%).
  • Step 1 Synthesis of YK-CAP-109-PM1
  • YK-CAP-107-PM3 (10.0 g, 21.90 mmol) was dissolved in dichloromethane.
  • the reaction system was cooled to 0°C, and DAST (7.1 g, 43.80 mmol) was slowly added dropwise thereto.
  • the reaction system was stirred and reacted at 0°C for 4 hours.
  • the system was slowly added with saturated sodium bicarbonate aqueous solution to quench the reaction, then added with DCM, stirred, and the phases were separated.
  • the organic phase was washed twice with saturated sodium bicarbonate aqueous solution, and the phases were separated.
  • Step 2 Synthesis of YK-CAP-109-PM2
  • YK-CAP-109-PM1 (6.1 g, 13.30 mmol) was dissolved in acetic acid, then acetic anhydride (27.2 g, 266.0 mmol) was added thereto, and concentrated sulfuric acid (280 ⁇ L) was slowly added dropwise thereto. After the dropwise addition was completed, the mixture was stirred at room temperature for 16 hours. The reaction mixture was added with 200 mL of water and extracted with ethyl acetate.
  • Step 3 Synthesis of YK-CAP-109-PM3
  • the reaction system was diluted with ethyl acetate, washed once with saturated sodium bicarbonate solution, filtered to remove the insoluble substance, and the phases of the filtrate were separated. The organic phase was dried over anhydrous sodium sulfate, and evaporated by rotary evaporation under reduced pressure to remove the solvent. The residue was purified by silica gel column chromatography (0 to 30% ethyl acetate/DCM) to obtain YK-CAP-109-PM3 (3.1 g, 4.88 mmol). C 31 H 31 FN 6 O 8 , MS (ES): m/z (M+H + ) 635.2.
  • Step 4 Synthesis of YK-CAP-109-PM4
  • YK-CAP-109-PM3 (3.1 g, 4.88 mmol) was dissolved in 7 M ammonia/methanol solution and water (5:1), and the mixture was stirred and reacted at room temperature for 16 hours. The reaction mixture was evaporated to dryness by rotary evaporation to remove the solvent, and the crude product was recrystallized with ethyl acetate to obtain YK-CAP-109-PM4 (1.1 g, 3.51 mmol, 71.9%). C 12 H 16 FN 5 O 4 , MS (ES): m/z (M+H + ) 314.1.
  • Step 5 Synthesis of YK-CAP-109-PM5
  • Phosphorus oxychloride (1.6 g, 10.5 mmol) was dissolved in 20 mL of trimethyl phosphate. The mixture was cooled to 0°C under nitrogen atmosphere. The above reaction system was slowly added with YK-CAP-109-PM4 (1.1 g, 3.51 mmol), and stirred and reacted at 0°C for about 4 hours. After the reaction was completed, the reaction mixture was added with 20 mL of ice water and washed twice with ethyl acetate. The aqueous phase was added with ammonia water to adjust the pH to 3.5, and stored in a refrigerator overnight. The next day, the pH was continuously adjusted to 6.5, and the mixture was diluted to 400 mL for sample loading.
  • the sample was purified by gel column chromatography (eluted with water and 1.5 M TEAB at a ratio of 1:4).
  • the target product peak was collected, concentrated, and lyophilized to obtain YK-CAP-109-PM5 (triethylamine salt, 980 mg, 1.98 mmol, 56.5%) as a white solid.
  • Step 6 Synthesis of YK-CAP-109-PM6
  • YK-CAP-109-PM5 (980 mg, 1.98 mmol) was used as starting material to obtain YK-CAP-109-PM6 (sodium salt, 900 mg, 1.93 mmol, 97.6%).
  • YK-CAP-109-PM6 sodium salt, 900 mg, 1.93 mmol, 97.6%.
  • Step 7 Synthesis of YK-CAP-109-PM7
  • YK-CAP-109-PM6 (900 mg, 1.93 mmol) was used as starting material to obtain YK-CAP-109-PM7 (triethylamine salt, 850 mg, 1.48 mmol, 76.7%).
  • YK-CAP-109-PM7 triethylamine salt, 850 mg, 1.48 mmol, 76.7%.
  • Step 8 Synthesis of YK-CAP-109-PM8
  • YK-CAP-101-PM6 YK-CAP-109-PM7 (850 mg, 1.48 mmol) was used as starting material to obtain YK-CAP-109-PM8 (triethylamine salt, 450 mg, 0.76 mmol, 51.7%).
  • Step 9 Synthesis of YK-CAP-109
  • Step 1 Synthesis of YK-CAP-110-PM1
  • YK-CAP-107-PM2 (14.4 g, 32.68 mmol) was dissolved in dichloromethane.
  • the reaction system was cooled to 0°C, and DAST (16.7 g, 103.5 mmol) was slowly added dropwise thereto.
  • the reaction system was stirred and reacted at 0°C for 4 hours.
  • the system was slowly added with saturated sodium bicarbonate aqueous solution to quench the reaction, then added with DCM, stirred, and the phases were separated.
  • the organic phase was washed twice with saturated sodium bicarbonate aqueous solution, and the phases were separated.
  • Step 2 Synthesis of YK-CAP-110-PM2
  • YK-CAP-110-PM1 (5.6 g, 12.11 mmol) was dissolved in acetic acid, then acetic anhydride (24.7 g, 242.1 mmol) was added thereto, and concentrated sulfuric acid (280 ⁇ L) was slowly added dropwise thereto. After the dropwise addition was completed, the mixture was stirred at room temperature for 16 hours. The reaction mixture was added with 200 mL of water and extracted with ethyl acetate.
  • Step 3 Synthesis of YK-CAP-110-PM3
  • the reaction system was diluted with ethyl acetate, washed once with saturated sodium bicarbonate solution, filtered to remove the insoluble substance, and the phases of the filtrate were separated. The organic phase was dried over anhydrous sodium sulfate, and evaporated by rotary evaporation under reduced pressure to remove the solvent. The residue was purified by silica gel column chromatography (0 to 30% ethyl acetate/DCM) to obtain YK-CAP-110-PM3 (3.5 g, 5.48 mmol). C 30 H 28 F 2 N 6 O 8 , MS (ES): m/z (M+H + ) 639.1.
  • Step 4 Synthesis of YK-CAP-110-PM4
  • YK-CAP-110-PM3 (3.5 g, 5.48 mmol) was dissolved in 4 M ammonia/methanol solution and water (5:1), and the mixture was stirred and reacted at room temperature for 16 hours. The reaction mixture was evaporated to dryness by rotary evaporation to remove the solvent, and the crude product was recrystallized with ethyl acetate to obtain YK-CAP-110-PM4 (1.3 g, 4.10 mmol, 74.7%). C 11 H 13 F 2 N 5 O 4 , MS (ES): m/z (M+H + ) 318.1.
  • YK-CAP-110-PM4 1 H NMR (400 MHz, DMSO- d 6 ) ⁇ 10.56 (s, 1H), 8.00 (s, 1H), ⁇ 6.40 (s, 2H), 5.71 - 5.56 (m, 2H), 4.86 - 4.83 (m, 1H), 4.36 - 4.32 (m, 1H), 4.19 - 4.15 (m, 1H), 4.62 (s, 1H), 3.75 - 3.73 (m, 1H), 3.62 - 3.49 (m, 1H), 3.27 - 3.22 (m, 1H).
  • Step 5 Synthesis of YK-CAP-110-PM5
  • Phosphorus oxychloride (1.9 g, 12.3 mmol) was dissolved in 20 mL of trimethyl phosphate. The mixture was cooled to 0°C under nitrogen atmosphere, and YK-CAP-110-PM4 (1.3 g, 4.10 mmol) was slowly added to the above reaction system. The mixture was stirred and reacted at 0°C for about 4 hours. After the reaction was completed, the reaction mixture was added with 20 mL of ice water and washed twice with ethyl acetate. The aqueous phase was added with ammonia water to adjust the pH to 3.5, and stored in a refrigerator overnight. The next day, the pH was continuously adjusted to 6.5, and the mixture was diluted to 400 mL for sample loading.
  • the sample was purified by gel column chromatography (eluted with water and 1.5 M TEAB at a ratio of 1:5).
  • the target product peak was collected, concentrated, and lyophilized to obtain YK-CAP-110-PM5 (triethylamine salt, 1.1 g, 2.21 mmol, 53.8%) as a white solid.
  • Step 6 Synthesis of YK-CAP-110-PM6
  • Step 7 Synthesis of YK-CAP-110-PM7
  • YK-CAP-101-PM5 YK-CAP-110-PM6 (1.0 g, 2.13 mmol) was used as starting material to obtain YK-CAP-110-PM7 (triethylamine salt, 900 mg, 1.56 mmol, 73.1%).
  • Step 8 Synthesis of YK-CAP-110-PM8
  • YK-CAP-101-PM6 YK-CAP-110-PM7 (900 mg, 1.56 mmol) was used as starting material to obtain YK-CAP-110-PM8 (triethylamine salt, 500 mg, 0.84 mmol, 54.1%).
  • Step 9 Synthesis of YK-CAP-110
  • YK-CAP-110-PM8 100 mg, 0.17 mmol was used as starting material to obtain YK-CAP-110 (25 mg, 22.03 ⁇ mol, 13.0%).
  • Step 1 Synthesis of YK-CAP-111-PM1
  • YK-CAP-107-PM1 (35.0 g, 79.07 mmol) was dissolved in acetonitrile and water (1:1, 280 mL), then (diacetoxyiodo)benzene (53.5 g, 166.03 mmol), sodium bicarbonate (9.96 g, 118.56 mmol), and TEMPO (1.85 g, 11.85 mmol) were sequentially added thereto in an ice bath, and the mixture was reacted at room temperature for 2 hours. TLC monitored that the starting material was completely reacted. The reaction was terminated. The reaction mixture was quenched with saturated sodium thiosulfite aqueous solution, extracted with EA, and the phases were separated.
  • Step 2 Synthesis of YK-CAP-111-PM2
  • YK-CAP-111-PM1 (34.0 g, calculated as 39.54 mmol) was dissolved in acetonitrile, and the mixture was cooled to 0°C. DIEA (14.0 g, 108.33 mmol) and HATU (17.86 g, 46.97 mmol) were added thereto. The mixture was stirred for 20 minutes, and then diethylamine (6.6 g, 90.24 mmol) was added thereto. The mixture was warmed to room temperature, and stirred and reacted for 4 hours.
  • reaction mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (0 to 20% ethyl acetate/n-hexane) to obtain YK-CAP-111-PM2 (17.5 g, 34.20 mmol, 86.5%).
  • Step 3 Synthesis of YK-CAP-111-PM3
  • YK-CAP-111-PM2 (17.5 g, 34.20 mmol) was dissolved in tetrahydrofuran, then TBAF (13.4 g, 51.25 mmol) was added thereto, and the mixture was stirred and reacted at room temperature for 2 hours.
  • the reaction mixture was evaporated to dryness by rotary evaporation, and the residue was purified by silica gel column chromatography (0 to 90% ethyl acetate/n-hexane) to obtain YK-CAP-111-PM3 (8.9 g, 32.56 mmol, 95.2%).
  • Step 4 Synthesis of YK-CAP-111-PM4
  • YK-CAP-111-PM3 (4.0 g, 14.63 mmol) was dissolved in acetic acid, then sulfuric acid (300 ⁇ L) was added thereto, and the mixture was stirred at room temperature for 30 minutes.
  • Acetic anhydride (30.0 g, 293.86 mmol) was then added thereto. The mixture was stirred at room temperature for 18 hours. The reaction mixture was added with 200 mL of water and extracted with ethyl acetate.
  • Step 5 Synthesis of YK-CAP-111-PM5
  • the reaction system was diluted with ethyl acetate, washed once with saturated sodium bicarbonate solution, filtered to remove the insoluble substance, and the phases of the filtrate were separated.
  • the organic phase was dried over anhydrous sodium sulfate, and evaporated by rotary evaporation under reduced pressure to remove the solvent.
  • the residue was purified by silica gel column chromatography (0 to 60% ethyl acetate/DCM) to obtain YK-CAP-111-PM5 (2.20 g, 3.20 mmol).
  • Step 6 Synthesis of YK-CAP-111-PM6
  • YK-CAP-111-PM5 (2.20 g, 3.20 mmol) was dissolved in 4 M ammonia/methanol solution and water (5:1), and the mixture was stirred and reacted at room temperature for 16 hours. The reaction mixture was evaporated to dryness by rotary evaporation to remove the solvent, and the crude product was recrystallized with ethyl acetate to obtain YK-CAP-111-PM6 (890 mg, 2.43 mmol, 75.9%). C 15 H 22 N 6 O 5 , MS (ES): m/z (M+H + ) 367.1.
  • Step 7 Synthesis of YK-CAP-111-PM7
  • Phosphorus oxychloride (1.3 g, 8.48 mmol) was dissolved in 15 mL of trimethyl phosphate. The mixture was cooled to 0°C under nitrogen atmosphere, and YK-CAP-111-PM6 (880 mg, 2.42 mmol) was slowly added to the above reaction system. The mixture was stirred and reacted at 0°C for about 4 hours. After the reaction was completed, the reaction mixture was added with 20 mL of ice water and washed twice with ethyl acetate. The aqueous phase was added with ammonia water to adjust the pH to 3.5, and refrigerated overnight. The pH was continuously adjusted to 6.5, and the mixture was diluted to 400 mL for sample loading.
  • the sample was purified by gel column chromatography (eluted with water and 1.5 M TEAB at a ratio of 5:1).
  • the target product peak was collected, concentrated, and lyophilized to obtain YK-CAP-111-PM7 (triethylamine salt, 900 mg, 1.64 mmol, 68.5%) as a white solid.
  • Step 8 Synthesis of YK-CAP-111-PM8
  • YK-CAP-111-PM7 (900 mg, 1.64 mmol) was used as starting material to obtain YK-CAP-111-PM8 (sodium salt, 730 mg, 1.41 mmol, 85.7%).
  • Step 9 Synthesis of YK-CAP-111-PM9
  • YK-CAP-111-PM8 (720 mg, 1.39 mmol) was used as starting material to obtain YK-CAP-111-PM9 (triethylamine salt, 750 mg, 1.20 mmol, 86.1%).
  • Step 10 Synthesis of YK-CAP-111-PM10
  • YK-CAP-111-PM9 400 mg, 0.64 mmol was dissolved in H 2 O (20 mL), and the mixture was added with glacial acetic acid to adjust the pH to 4.0. Dimethyl sulfate (800 ⁇ L, 8.45 mmol) was added thereto within 30 minutes.
  • the reaction system was added with NaOH aqueous solution (0.1 M) to maintain the pH between 3.8 and 4.1, and stirred at room temperature for 5 hours. After the reaction was completed, the reaction mixture was extracted twice with dichloromethane. The pH of the aqueous phase was adjusted to 6.5, and the volume was fixed to 400 mL.
  • Step 11 Synthesis of YK-CAP-111
  • YK-CAP-111-PM10 100 mg, 0.16 mmol was used as starting material to obtain YK-CAP-111 (ammonium salt, 50 mg, 39.07 ⁇ mol, 25.1%).
  • Step 1 Synthesis of YK-CAP-112-PM1
  • YK-CAP-111-PM1 (10.0 g, 21.90 mmol) and dipropylamine (5.5 g, 54.35 mmol) were used as starting materials to obtain YK-CAP-112-PM1 (11.0 g, 20.38 mmol, 93.1%).
  • YK-CAP-112-PM1 (11.0 g, 20.38 mmol) was used as starting material to obtain YK-CAP-112-PM2 (6.0 g, 19.91 mmol, 97.7%).
  • YK-CAP-112-PM2 (6.0 g, 19.91 mmol) was used as starting material to obtain the crude product YK-CAP-112-PM3 (5.1 g) as a yellow oily liquid, which was directly used in the next reaction step.
  • YK-CAP-112-PM4 (3.9 g, 5.45 mmol) was used as starting material to obtain YK-CAP-112-PM5 (1.7 g, 4.31 mmol, 79.1%).
  • YK-CAP-112-PM5 (1.7 g, 4.31 mmol) was used as starting material to obtain YK-CAP-112-PM6 (triethylamine salt, 1.80 g, 3.13 mmol, 72.6%).
  • Step 7 Synthesis of YK-CAP-112-PM7
  • YK-CAP-112-PM6 (1.8 g, 3.13 mmol) was used as starting material to obtain YK-CAP-112-PM7 (sodium salt, 1.47 g, 2.69 mmol, 86.0%).
  • YK-CAP-112-PM7 sodium salt, 1.47 g, 2.69 mmol, 86.0%.
  • Step 8 Synthesis of YK-CAP-112-PM8
  • YK-CAP-112-PM7 (1.47 g, 2.69 mmol) was used as starting material to obtain YK-CAP-112-PM8 (triethylamine salt, 1.30 g, 1.98 mmol, 73.7%).
  • YK-CAP-112-PM8 600 mg, 0.92 mmol was used as starting material to obtain YK-CAP-112-PM9 (triethylamine salt, 200 mg, 0.30 mmol, 32.6%).
  • YK-CAP-112-PM9 triethylamine salt, 200 mg, 0.30 mmol, 32.6%.
  • YK-CAP-112-PM9 200 mg, 0.30 mmol was used as starting material to obtain YK-CAP-112 (ammonium salt, 50 mg, 38.23 ⁇ mol, 12.8%).
  • YK-CAP-112 ammonium salt, 50 mg, 38.23 ⁇ mol, 12.8%.
  • Step 1 Synthesis of YK-CAP-113-PM1
  • Methyl-beta-D-ribofuranoside (100.0 g, 0.61 mol) was dissolved in 1 L of anhydrous pyridine, and TIPDSCl (230.6 g, 0.73 mol) was added dropwise thereto in an ice-water bath. After the dropwise addition was completed, the mixture was warmed to room temperature, and stirred and reacted for 12 hours. The reaction mixture was concentrated under reduced pressure to remove a large amount of solvent, and the residue was purified by silica gel column chromatography (0 to 30% ethyl acetate/n-hexane) to obtain YK-CAP-113-PM1 (212.2 g, 0.52 mol, 85.7%).
  • Step 2 Synthesis of YK-CAP-113-PM2
  • YK-CAP-113-PM1 (212.2 g, 0.52 mol) was dissolved in 2 L of acetonitrile, and Dess-Martin periodinane (485.1 g, 1.14 mol) was added thereto. The mixture was heated to 40°C, and stirred and reacted for 12 hours. The reaction mixture was cooled to room temperature, filtered, and the filtrate was evaporated to dryness by rotary evaporation under reduced pressure to obtain YK-CAP-113-PM2 (208.8 g, 0.52 mol, 98.9%).
  • Step 3 Synthesis of YK-CAP-113-PM3
  • Step 4 Synthesis of YK-CAP-113-PM4
  • the reaction system was re-cooled to 0°C, and water/tetrahydrofuran (1:1) (540 mL), 2 N sodium hydroxide solution (540 mL), and 30% hydrogen peroxide (460 mL) were sequentially and slowly added thereto. After the addition was completed, the reaction system was warmed to room temperature, and stirred and reacted for another 3 hours.
  • the reaction system was diluted with ethyl acetate, and the phases were separated. The aqueous phase was back-extracted once with ethyl acetate.
  • the organic phases were combined, dried, filtered, and the filtrate was evaporated to dryness by rotary evaporation under reduced pressure.
  • the residue was purified by silica gel column chromatography (0 to 17% ethyl acetate/n-hexane) to obtain YK-CAP-113-PM4 (98.0 g, 0.23 mol, 86.2%).
  • Step 5 Synthesis of YK-CAP-113-PM5
  • YK-CAP-113-PM4 (20.0 g, 47.54 mmol) and sodium hydride (1369 mg, 57.04 mmol) were dissolved in dry tetrahydrofuran (100 mL), and the mixture was cooled to 0°C. Iodomethane (13.5 g, 95.11 mmol) was then added dropwise thereto under nitrogen atmosphere, and the mixture was stirred and reacted for about 5 hours. After the reaction was completed, the system was quenched with water (10 mL) and extracted with EA (200 mL ⁇ 3).
  • Step 6 Synthesis of YK-CAP-113-PM6
  • Benzoyladenosine (39.2 g, 163.85 mmol) was dissolved in hexamethyldisilazane (500 mL), and a catalytic amount of ammonium sulfate was added thereto. The mixture was heated to 130°C under nitrogen atmosphere, stirred for 12 hours, and evaporated to dryness by rotary evaporation to remove the solvent.
  • YK-CAP-113-PM5 17.8 g, 40.95 mmol
  • 1,2-dichloroethane 300 mL
  • Trimethylsilyl trifluoromethanesulfonate (10.1 g, 48.97 mmol) was then added thereto, and the mixture was reacted at 80°C for 5 hours. TLC monitored that the reaction was complete. After filtration, the filtrate was evaporated to dryness by rotary evaporation under reduced pressure. The residue was purified by silica gel column chromatography (0 to 80% ethyl acetate/n-hexane) to obtain YK-CAP-113-PM6 (14.5 g, 22.59 mmol, 55.2%). C 31 H 47 N 5 O 6 Si 2 , MS (ES): m/z (M+H + ) 642.3.
  • Step 7 Synthesis of YK-CAP-113-PM7
  • YK-CAP-113-PM6 (14.5 g, 22.59 mmol) was dissolved in tetrahydrofuran (100 mL), then tetrabutylammonium fluoride (23.6 g, 90.26 mmol) was added thereto, and the mixture was stirred and reacted at room temperature for 1 hour.
  • the reaction system was added with saturated ammonium chloride aqueous solution and extracted with ethyl acetate (200 mL ⁇ 3). The organic phases were combined, washed with saturated NaCl aqueous solution, dried over anhydrous sodium sulfate, filtered, and evaporated to dryness by rotary evaporation.
  • Step 8 Synthesis of YK-CAP-113-PM8
  • YK-CAP-113-PM7 (7.5 g, 18.78 mmol) was dissolved in pyridine (50 mL), then 4,4'-dimethoxytrityl chloride (10.2 g, 30.10 mmol) was added thereto at room temperature, and the mixture was stirred and reacted at room temperature for 3 hours. TLC monitored that the reaction was complete. The reaction mixture was quenched with 10 mL of methanol, stirred for 10 minutes, and subjected to rotary evaporation until no solvent was evaporated to obtain a crude product. The crude product was purified by flash column chromatography to obtain YK-CAP-113-PM8 (8.8 g, 12.54 mmol, 66.8%). C 40 H 39 N 5 O 7 , MS (ES): m/z (M+H + ) 702.3.
  • Step 9 Synthesis of YK-CAP-113-PM9
  • YK-CAP-113-PM8 (8.8 g, 12.54 mmol) was dissolved in acetonitrile (100 mL), then N-methylimidazole (1.1 g, 13.40 mmol) and bis(diisopropylamino)(2-cyanoethoxy)phosphine (11.3 g, 37.49 mmol) were sequentially added thereto, and the mixture was stirred and reacted for 6 hours under nitrogen atmosphere. TLC monitored that the reaction was complete. The reaction mixture was diluted with ethyl acetate. The organic phase was washed with saturated sodium bicarbonate aqueous solution, and the phases were separated. The organic phase was washed once with water, and the phases were separated.
  • Step 10 Synthesis of YK-CAP-113-PM10
  • YK-CAP-113-PM9 (4.0 g, 4.43 mmol), N-isobutyryl-2',3'-acetylguanosine (2.0 g, 4.57 mmol), and tetrazole (3.1 g, 44.25 mmol) were dissolved in acetonitrile (50 mL), and the mixture was stirred and reacted at room temperature for 3 hours under nitrogen atmosphere. The above reaction mixture was added with 0.1 M iodine solution (53 mL), stirred and reacted for another 1 hour, diluted with 200 mL of brine, and extracted with dichloromethane (200 mL ⁇ 3).
  • Step 11 Synthesis of YK-CAP-113-PM11
  • YK-CAP-113-PM10 (5.2 g, 4.15 mmol) was dissolved in 80% acetic acid aqueous solution (20 mL), and the mixture was stirred and reacted at room temperature for 2 hours under nitrogen atmosphere. The reaction system was concentrated under reduced pressure to remove acetic acid, and subjected to rotary evaporation until no solvent was evaporated to obtain a crude product. The crude product was purified by flash column chromatography to obtain YK-CAP-113-PM11 (3.0 g, 3.15 mmol, 76.0%). C 40 H 46 N 11 O 15 P, MS (ES): m/z (M-H - ) 950.3.
  • Step 12 Synthesis of YK-CAP-113-PM12
  • YK-CAP-113-PM11 (3.0 g, 3.15 mmol) was dissolved in acetonitrile (30 mL), then N-methylimidazole (0.5 g, 6.09 mmol) and bis(diisopropylamino)(2-cyanoethoxy)phosphine (2.8 g, 9.29 mmol) were sequentially added thereto, and the mixture was stirred and reacted for 2 hours under nitrogen atmosphere.
  • the above reaction mixture was added with 0.1 M iodine solution (40 mL), stirred and reacted for another 1 hour, diluted with 200 mL of brine, and extracted with dichloromethane (200 mL ⁇ 3).
  • Step 13 Synthesis of YK-CAP-113-PM13
  • YK-CAP-113-PM12 (1.8 g, 1.66 mmol) was dissolved in a mixed solution of ammonia water (10 mL) and methanol (5 mL). The mixture was heated to 50°C under nitrogen atmosphere, and stirred and reacted for 24 hours. The reaction system was concentrated under reduced pressure to remove the solvent, and subjected to rotary evaporation until no solvent was evaporated to obtain a crude product. The crude product was dissolved in water (50 mL) until clarified, and purified by gel column chromatography (eluted with water and 1.5 M TEAB at a ratio of 10:1).
  • the target product peak was collected, concentrated, lyophilized, and further desalted by preparative high performance liquid chromatography (50 mM TEAB and methanol mobile phase system) to obtain YK-CAP-113-PM13 (triethylamine salt, 660 mg, 0.80 mmol, 48.4%) as a white solid.
  • Step 14 Synthesis of YK-CAP-113
  • YK-CAP-113-PM11 150 mg, 0.18 mmol
  • Im-m7GDP (193 mg, 0.36 mmol) were used as starting materials to obtain YK-CAP-113 (ammonium salt, 40 mg, 33.04 ⁇ mol, 18.1%).
  • Step 1 Synthesis of YK-CAP-114-PM1
  • YK-CAP-113-PM4 (13.3 g, 31.61 mmol), phthalimide (5.6 g, 38.06 mmol), triphenylphosphine (16.6 g, 63.29 mmol), and DIAD (7.7 g, 38.08 mmol) were dissolved in dry tetrahydrofuran (150 mL). The mixture was cooled to 0°C, and stirred and reacted for about 5 hours under nitrogen atmosphere. After the reaction was completed, the system was quenched with water (10 mL) and extracted with EA (200 mL ⁇ 3).
  • Step 2 Synthesis of YK-CAP-114-PM2
  • YK-CAP-114-PM1 (15.9 g, 28.92 mmol) was dissolved in 300 mL of ethanol, and 50 mL of hydrazine hydrate was added thereto. The mixture was heated to 80°C, and stirred and reacted for 12 hours. After the reaction was completed, the system was quenched with water (10 mL) and extracted with EA (200 mL ⁇ 3). The organic phases were combined, washed with saturated NaCl aqueous solution, dried over anhydrous sodium sulfate, filtered, and evaporated to dryness by rotary evaporation to obtain YK-CAP-114-PM2 (12.0 g, 28.59 mmol, 98.9%). C 19 H 41 NO 5 Si 2 , MS (ES): m/z (M+H + ) 420.3.
  • Step 3 Synthesis of YK-CAP-114-PM3
  • YK-CAP-114-PM2 (12.0 g, 28.59 mmol) and triethylamine (5.9 g, 58.31 mmol) were dissolved in dichloromethane (150 mL). The mixture was cooled to 0°C, and acetyl chloride (3.3 g, 42.04 mmol) was slowly added dropwise thereto. After the dropwise addition was completed, the reaction system was maintained at 0°C, and stirred and reacted for 3 hours. After the reaction was completed, the system was quenched with water (20 mL) and extracted with DCM (200 mL ⁇ 3).
  • Step 4 Synthesis of YK-CAP-114-PM4
  • YK-CAP-113-PM6 YK-CAP-114-PM3 (12.2 g, 26.42 mmol) was used as starting material to obtain YK-CAP-114-PM4 (8.5 g, 12.71 mmol, 48.1%).
  • Step 5 Synthesis of YK-CAP-114-PM5
  • YK-CAP-114-PM4 (8.5 g, 12.71 mmol) was used as starting material to obtain YK-CAP-114-PM5 (4.4 g, 10.32 mmol, 81.2%).
  • Step 6 Synthesis of YK-CAP-114-PM6
  • YK-CAP-114-PM5 (4.4 g, 10.32 mmol) was used as starting material to obtain YK-CAP-114-PM6 (5.5 g, 7.55 mmol, 73.1%).
  • Step 7 Synthesis of YK-CAP-114-PM7
  • YK-CAP-114-PM6 (5.5 g, 7.55 mmol) was used as starting material to obtain YK-CAP-114-PM7 (5.0 g, 5.38 mmol, 71.3%).
  • Step 8 Synthesis of YK-CAP-114-PM8
  • YK-CAP-114-PM7 (5.0 g, 5.38 mmol) was used as starting material to obtain YK-CAP-114-PM8 (5.7 g, 4.45 mmol, 82.7%).
  • Step 9 Synthesis of YK-CAP-114-PM9
  • YK-CAP-114-PM8 (5.7 g, 4.45 mmol) was used as starting material to obtain YK-CAP-114-PM9 (3.5 g, 3.58 mmol, 80.4%).
  • Step 10 Synthesis of YK-CAP-114-PM10
  • YK-CAP-114-PM9 (3.5 g, 3.58 mmol) was used as starting material to obtain YK-CAP-114-PM10 (2.0 g, 1.80 mmol, 50.3%).
  • Step 11 Synthesis of YK-CAP-114-PM11
  • YK-CAP-114-PM10 (2.0 g, 1.80 mmol) was used as starting material to obtain YK-CAP-114-PM11 (triethylamine salt, 800 mg, 0.94 mmol, 52.4%).
  • Step 12 Synthesis of YK-CAP-114
  • YK-CAP-114-PM11 150 mg, 0.18 mmol was used as starting material to obtain YK-CAP-114 (ammonium salt, 26 mg, 21.01 ⁇ mol, 11.9%).
  • Step 1 Synthesis of YK-CAP-115-PM1
  • YK-CAP-113-PM4 (20.0 g, 47.54 mmol) was dissolved in dichloromethane (200 mL). The mixture was cooled to -40°C, and a solution of diethylaminosulfur trifluoride (9.2 g, 57.08 mmol) in dichloromethane (20 mL) was slowly added dropwise thereto. After the dropwise addition was completed, the mixture was slowly warmed to 0°C, and stirred and reacted for 4 hours. TLC monitored that the reaction was complete. The reaction system was quenched with saturated sodium bicarbonate aqueous solution (100 mL) and extracted with dichloromethane (200 mL ⁇ 3).
  • Step 2 Synthesis of YK-CAP-115-PM2
  • YK-CAP-115-PM1 (11.2 g, 26.50 mmol) was used as starting material to obtain YK-CAP-115-PM2 (8.6 g, 13.65 mmol, 51.5%).
  • Step 3 Synthesis of YK-CAP-115-PM3
  • YK-CAP-115-PM2 (8.6 g, 13.65 mmol) was used as starting material to obtain YK-CAP-115-PM3 (4.8 g, 12.39 mmol, 90.8%).
  • Step 4 Synthesis of YK-CAP-115-PM4
  • YK-CAP-115-PM3 (4.8 g, 12.39 mmol) was used as starting material to obtain YK-CAP-115-PM4 (5.6 g, 8.12 mmol, 65.5%).
  • Step 5 Synthesis of YK-CAP-115-PM5
  • YK-CAP-115-PM4 (5.6 g, 8.12 mmol) was used as starting material to obtain YK-CAP-115-PM5 (5.0 g, 5.62 mmol, 69.2%).
  • Step 6 Synthesis of YK-CAP-115-PM6
  • YK-CAP-115-PM5 (5.0 g, 5.62 mmol) was used as starting material to obtain YK-CAP-115-PM6 (4.9 g, 3.94 mmol, 70.2%).
  • Step 7 Synthesis of YK-CAP-115-PM7
  • YK-CAP-115-PM6 (4.9 g, 3.94 mmol) was used as starting material to obtain YK-CAP-115-PM7 (2.3 g, 2.45 mmol, 62.0%).
  • Step 8 Synthesis of YK-CAP-115-PM8
  • YK-CAP-115-PM7 (2.3 g, 2.45 mmol) was used as starting material to obtain YK-CAP-115-PM8 (2.0 g, 1.86 mmol, 76.2%).
  • Step 9 Synthesis of YK-CAP-115-PM9
  • YK-CAP-115-PM8 (2.0 g, 1.86 mmol) was used as starting material to obtain YK-CAP-115-PM9 (triethylamine salt, 720 mg, 0.89 mmol, 47.7%).
  • YK-CAP-115-PM9 triethylamine salt, 720 mg, 0.89 mmol, 47.7%.
  • Step 10 Synthesis of YK-CAP-115
  • YK-CAP-115-PM9 150 mg, 0.19 mmol was used as starting material to obtain YK-CAP-115 (ammonium salt, 31 mg, 25.86 ⁇ mol, 14.0%).
  • YK-CAP-115 ammonium salt, 31 mg, 25.86 ⁇ mol, 14.0%.
  • Step 1 Synthesis of YK-CAP-116-PM1
  • YK-CAP-113-PM4 (20.0 g, 47.54 mmol) was dissolved in acetonitrile (200 mL), and 2-iodoxybenzoic acid (16.0 g, 57.14 mmol) was added thereto. The mixture was heated to 90°C, and stirred and reacted for 4 hours. The reaction mixture was cooled to room temperature, filtered, and the filtrate was evaporated to dryness by rotary evaporation under reduced pressure to obtain YK-CAP-116-PM1 (18.4 g, 43.95 mmol, 92.4%).
  • Step 2 Synthesis of YK-CAP-116-PM2
  • YK-CAP-116-PM1 (18.4 g, 43.95 mmol) was dissolved in dichloromethane (200 mL).
  • the reaction system was cooled to -40°C, and a solution of diethylaminosulfur trifluoride (21.3 g, 132.14 mmol) in dichloromethane (40 mL) was slowly added dropwise thereto. After the dropwise addition was completed, the reaction system was slowly warmed to 0°C, and stirred and reacted for 4 hours. TLC monitored that the reaction was complete.
  • the reaction system was quenched with saturated sodium bicarbonate aqueous solution (200 mL) and extracted with dichloromethane (300 mL ⁇ 3).
  • Step 3 Synthesis of YK-CAP-116-PM3
  • YK-CAP-113-PM6 (12.8 g, 29.05 mmol) was used as starting material to obtain YK-CAP-116-PM3 (9.6 g, 14.82 mmol, 51.0%).
  • Step 4 Synthesis of YK-CAP-116-PM4
  • YK-CAP-116-PM3 (9.6 g, 14.82 mmol) was used as starting material to obtain YK-CAP-116-PM4 (5.2 g, 12.83 mmol, 86.6%).
  • Step 5 Synthesis of YK-CAP-116-PM5
  • YK-CAP-113-PM8 According to the synthesis method of YK-CAP-113-PM8, YK-CAP-116-PM4 (5.2 g, 12.83 mmol) was used as starting material to obtain YK-CAP-116-PM5 (6.4 g, 9.04 mmol, 70.5%).
  • Step 6 Synthesis of YK-CAP-116-PM6
  • YK-CAP-116-PM5 (6.4 g, 9.04 mmol) was used as starting material to obtain YK-CAP-116-PM6 (5.5 g, 6.06 mmol, 67.0%).
  • Step 7 Synthesis of YK-CAP-116-PM7
  • YK-CAP-113-PM10 According to the synthesis method of YK-CAP-113-PM10, YK-CAP-116-PM6 (5.5 g, 6.06 mmol) was used as starting material to obtain YK-CAP-116-PM7 (5.3 g, 4.21 mmol, 69.4%).
  • Step 8 Synthesis of YK-CAP-116-PM8
  • Step 9 Synthesis of YK-CAP-116-PM9
  • YK-CAP-116-PM8 (2.8 g, 2.92 mmol) was used as starting material to obtain YK-CAP-116-PM9 (2.2 g, 2.02 mmol, 69.0%).
  • Step 10 Synthesis of YK-CAP-116-PM10
  • YK-CAP-116-PM9 (2.2 g, 2.02 mmol) was used as starting material to obtain YK-CAP-116-PM10 (triethylamine salt, 580 mg, 0.70 mmol, 34.7%).
  • Step 11 Synthesis of YK-CAP-116
  • YK-CAP-116-PM10 150 mg, 0.18 mmol was used as starting material to obtain YK-CAP-116 (ammonium salt, 17 mg, 13.97 ⁇ mol, 7.7%).
  • Step 1 Synthesis of YK-CAP-117-PM1
  • Step 2 Synthesis of YK-CAP-117-PM2
  • YK-CAP-117-PM1 (8.62 g, 20.80 mmol) was dissolved in acetic acid, then acetic anhydride (21.23 g, 207.95 mmol) was added thereto, and concentrated sulfuric acid (380 ⁇ L) was slowly added dropwise thereto. After the dropwise addition was completed, the mixture was stirred at room temperature for 16 hours. The reaction mixture was added with 300 mL of water and extracted with ethyl acetate. The organic phase was washed three times with saturated sodium bicarbonate aqueous solution to adjust the pH to alkalinity, then dried, and evaporated to dryness by rotary evaporation.
  • Step 3 Synthesis of YK-CAP-117-PM3
  • YK-CAP-117-PM2 (8.13 g, 17.73 mmol) was used as starting material to obtain YK-CAP-117-PM3 (6.24 g, 7.93 mmol, 44.7%).
  • Step 4 Synthesis of YK-CAP-117-PM4
  • YK-CAP-117-PM3 (6.24 g, 7.93 mmol) was dissolved in dichloromethane (200 mL).
  • dichloromethane 200 mL
  • the above reaction system was cooled to -40°C under nitrogen atmosphere, and a 1 M solution of boron trichloride (79.3 mL, 79.30 mmol) in dichloromethane was slowly added dropwise thereto. After the dropwise addition was completed, the reaction system was slowly warmed to 0°C and stirred at the same temperature for 3 hours. TLC showed that the reaction was complete.
  • YK-CAP-117-PM4 1 H NMR (400 MHz, MeOD) ⁇ 8.15 (s, 1H), 4.51 (s, 1H), 4.41 (s, 1H), 4.21 - 4.17 (m, 1H), 3.81 (s, 2H), 3.44 - 3.31 (m, 2H), 2.97 (s, 3H).
  • Step 5 Synthesis of YK-CAP-117-PM5
  • Step 6 Synthesis of YK-CAP-117-PM6
  • YK-CAP-117-PM5 (1.23 g, 2.42 mmol) was used as starting material to obtain YK-CAP-117-PM6 (sodium salt, 993 mg, 2.07 mmol, 85.6%).
  • Step 7 Synthesis of YK-CAP-117-PM7
  • YK-CAP-117-PM6 (993 mg, 2.07 mmol) was used as starting material to obtain YK-CAP-117-PM7 (triethylamine salt, 794 mg, 1.35 mmol, 65.1%).
  • Step 8 Synthesis of YK-CAP-117-PM8
  • Step 9 Synthesis of YK-CAP-117
  • YK-CAP-117-PM8 150 mg, 0.25 mmol was used as starting material to obtain YK-CAP-117 (ammonium salt, 26 mg, 20.95 ⁇ mol, 8.4%).
  • Step 1 Synthesis of YK-CAP-118-PM1
  • Step 2 Synthesis of YK-CAP-118-PM2
  • YK-CAP-118-PM1 (9.11 g, 22.64 mmol) was used as starting material to obtain YK-CAP-118-PM2 (7.18 g, 16.08 mmol, 71.0%).
  • Step 3 Synthesis of YK-CAP-118-PM3
  • YK-CAP-118-PM2 (7.18 g, 16.08 mmol) was used as starting material to obtain YK-CAP-118-PM3 (6.64 g, 8.57 mmol, 53.3%).
  • Step 4 Synthesis of YK-CAP-118-PM4
  • YK-CAP-118-PM3 (6.64 g, 8.57 mmol) was used as starting material to obtain YK-CAP-118-PM4 (2.31 g, 7.33 mmol, 85.5%).
  • YK-CAP-118-PM4 1 H NMR (400 MHz, MeOD) ⁇ 8.12 (s, 1H), 4.51 (s, 1H), 4.40 (s, 1H), 4.27 - 4.22 (m, 1H), 3.88 (s, 2H), 3.72 - 3.62 (m, 2H).
  • Step 5 Synthesis of YK-CAP-118-PM5
  • YK-CAP-101-PM3 YK-CAP-118-PM4 (1.30 g, 4.12 mmol) was used as starting material to obtain YK-CAP-118-PM5 (triethylamine salt, 1.15 g, 2.32 mmol, 56.2%).
  • YK-CAP-118-PM5 triethylamine salt, 1.15 g, 2.32 mmol, 56.2%.
  • Step 6 Synthesis of YK-CAP-118-PM6
  • YK-CAP-118-PM5 (1.15 g, 2.32 mmol) was used as starting material to obtain YK-CAP-118-PM6 (sodium salt, 870 mg, 1.86 mmol, 80.4%).
  • Step 7 Synthesis of YK-CAP-118-PM7
  • YK-CAP-118-PM6 870 mg, 1.86 mmol was used as starting material to obtain YK-CAP-118-PM7 (triethylamine salt, 664 mg, 1.15 mmol, 61.9%).
  • YK-CAP-118-PM7 triethylamine salt, 664 mg, 1.15 mmol, 61.9%.
  • Step 8 Synthesis of YK-CAP-118-PM8
  • YK-CAP-118-PM7 (664 mg, 1.15 mmol) was used as starting material to obtain YK-CAP-118-PM8 (triethylamine salt, 419 mg, 0.71 mmol, 61.6%).
  • YK-CAP-118-PM8 triethylamine salt, 419 mg, 0.71 mmol, 61.6%.
  • Step 9 Synthesis of YK-CAP-118
  • YK-CAP-118-PM8 150 mg, 0.25 mmol was used as starting material to obtain YK-CAP-118 (ammonium salt, 33 mg, 26.86 ⁇ mol, 10.7%).
  • YK-CAP-118 ammonium salt, 33 mg, 26.86 ⁇ mol, 10.7%.
  • Step 1 Synthesis of YK-CAP-119-PM1
  • Step 2 Synthesis of YK-CAP-119-PM2
  • YK-CAP-119-PM1 (10.21 g, calculated as 24.97 mmol) was used as starting material to obtain YK-CAP-119-PM2 (8.16 g, 19.41 mmol, 77.7%).
  • Step 3 Synthesis of YK-CAP-119-PM3
  • YK-CAP-119-PM2 (8.16 g, 19.41 mmol) was used as starting material to obtain YK-CAP-119-PM3 (6.55 g, 14.10 mmol, 72.7%).
  • Step 4 Synthesis of YK-CAP-119-PM4
  • YK-CAP-119-PM3 (6.55 g, 14.10 mmol) was used as starting material to obtain YK-CAP-119-PM4 (5.77 g, 7.28 mmol, 51.6%).
  • Step 5 Synthesis of YK-CAP-119-PM5
  • YK-CAP-119-PM5 1 H NMR (400 MHz, MeOD) ⁇ 8.24 (s, 1H), 4.77 (s, 1H), 4.56 (s, 1H), 4.51 (s, 1H), 4.27 - 4.22 (m, 1H), 3.72 - 3.62 (m, 2H).
  • Step 6 Synthesis of YK-CAP-119-PM6
  • Step 7 Synthesis of YK-CAP-119-PM7
  • YK-CAP-119-PM6 (1.29 g, 2.51 mmol) was used as starting material to obtain YK-CAP-119-PM7 (sodium salt, 991 mg, 2.04 mmol, 81.4%).
  • YK-CAP-119-PM7 sodium salt, 991 mg, 2.04 mmol, 81.4%.
  • Step 8 Synthesis of YK-CAP-119-PM8
  • YK-CAP-119-PM7 (991 mg, 2.04 mmol) was used as starting material to obtain YK-CAP-119-PM8 (triethylamine salt, 774 mg, 1.30 mmol, 63.8%).
  • Step 9 Synthesis of YK-CAP-119-PM9
  • YK-CAP-119-PM8 (774 mg, 1.30 mmol) was used as starting material to obtain YK-CAP-119-PM9 (triethylamine salt, 533 mg, 0.88 mmol, 67.3%).
  • Step 10 Synthesis of YK-CAP-119
  • YK-CAP-119-PM9 150 mg, 0.25 mmol was used as starting material to obtain YK-CAP-119 (ammonium salt, 32 mg, 25.67 ⁇ mol, 10.3%).
  • 5227-S was used as starting material to obtain 35 mg of compound 5227.
  • Example 2 In vitro transcription yield of mRNA and capping rate
  • the plasmid was first linearized with plasmid linearizing enzyme, and then the linearized plasmid was purified.
  • YK-CAP-101 to 119, compound 5227, and CAP-2'O-ethyl synthesized in Example 1 were respectively used as cap analogs.
  • the reaction system is shown in Table 2: Table 2: In vitro transcription reaction system System Amount T7 RNA polymerase 50U 10X buffer 2 ⁇ L 100mMATP 1 ⁇ L 100mMGTP 1 ⁇ L 100mMCTP 1 ⁇ L 100mMUTP 1 ⁇ L 100 mM cap analog 1 ⁇ L Nuclease inhibitor 20U Inorganic pyrophosphatase 0.05U Sterile enzyme-free water Supplemented to 20 ⁇ L DNA template 1 ⁇ g
  • the volume of materials required for the system was first calculated, and then sample addition was conducted.
  • the system was first added with sterile enzyme-free water, followed by the sequential addition of 10X buffer, NTPs, and cap analogs, mixed well, and gently centrifuged. Nuclease inhibitors, inorganic pyrophosphatase, T7RNA polymerase, and linearized DNA templates were then added thereto.
  • the system was mixed well, gently centrifuged, and incubated at 37°C. After 2 hours of incubation, the system was added with 1 U of DNase I and incubated at 37°C for another 30 minutes.
  • the mRNA precipitate was then washed with 75% ethanol, and after the ethanol was briefly evaporated to dryness, the mRNA was redissolved in sterile enzyme-free water.
  • the obtained mRNA was subjected to an annealing reaction with a probe.
  • the annealing reaction was performed in a PCR instrument: 95°C for 5 minutes; 65°C for 2 minutes; 55°C for 2 minutes; 40°C for 2 minutes; 22°C for 2 minutes.
  • Magnetic bead pretreatment and probe binding 100 ⁇ L of magnetic beads was placed on a magnetic frame for pretreatment. The magnetic bead solution was added with 120 ⁇ L of sample and incubated at room temperature for 30 minutes with slow mixing.
  • the mixture was added with 20 ⁇ L of RNase H (5 U/ ⁇ L) and incubated at 37°C for 3 hours with mixing every half hour. After incubation, the magnetic beads were washed and then added with 100 ⁇ L of 75% methanol heated to 80°C. The mixture was heated to 80°C on a heating plate, held for 3 minutes, then placed on a magnetic frame to aspirate the supernatant, and dried at room temperature for 45 minutes to a volume of 10 ⁇ L using a centrifugal evaporator. The sample was then resuspended in 50 ⁇ L of 100 ⁇ M EDTA/1% MeOH and ready for LC-MS analysis to determine the capping of RNA in the transcription reaction. Since there is a significant difference in molecular weight between capped and uncapped bases, the capping rate of mRNA transcription initiated by different cap analogs can be determined based on the difference in molecular mass.
  • the measurement results of the in vitro transcription yield of mRNA and capping rate show that the ribose-modified cap analogs of the present disclosure have a significant difference in the in vitro transcription yield of mRNA and capping rate. Compared to the ribose-modified cap analogs in the prior art, the ribose-modified cap analogs of the present disclosure show a significant increase in both in vitro transcription yield of mRNA and capping rate.
  • Table 3 The specific in vitro transcription yield of mRNA and capping rate are shown in Table 3.
  • Table 3 In vitro transcription yield of mRNA and capping rate Name Yield per unit template ( ⁇ g) Increase relative to 5227 (%) Capping rate (%) Increase relative to 5227 (%) YK-CAP-101 32.5 -75.8 42.8 -40.5 YK-CAP-102 44.1 -67.1 30.2 -58.0 YK-CAP-103 88.0 -34.4 71.1 -1.1 YK-CAP-104 73.2 -45.5 77.2 7.4 YK-CAP-105 82.3 -38.7 69.3 -3.6 YK-CAP-106 155.5 15.9 95.3 32.5 YK-CAP-107 170.7 27.2 97.3 35.3 YK-CAP-108 151.8 13.1 95.8 33.2 YK-CAP-109 168.1 25.3 95.2 32.4 YK-CAP-110 172.1 28.2 97.4 35.5 YK-CAP-111 173.5 29.3 98.1 36.4
  • YK-CAP-106 to 119 have significantly higher in vitro transcription yield of mRNA and capping rate than those of YK-CAP-101 to 105.
  • YK-CAP-111 has the highest transcription yield and capping rate, with the transcription yield being 5.3 times that of YK-CAP-101 (the lowest) and the capping rate being 3.2 times that of YK-CAP-102 (the lowest).
  • YK-CAP-107, YK-CAP-110, YK-CAP-111, YK-CAP-117, and YK-CAP-118 have a yield of 170.7 ⁇ g, 172.1 ⁇ g, 173.5 ⁇ g, 153.2 ⁇ g, and 172.2 ⁇ g, respectively, with YK-CAP-111 having the highest yield of 173.5 ⁇ g (as shown in FIG. 1 ).
  • YK-CAP-101 has the lowest in vitro transcription yield of mRNA, which is only 32.5 ⁇ g.
  • YK-CAP-102 to 105 also have a very low yield of 44.1 ⁇ g, 88.0 ⁇ g, 73.2 ⁇ g, and 82.3 ⁇ g, respectively.
  • the transcription yield of YK-CAP-111 is 5.3 times, 3.9 times, 2.0 times, 2.4 times, and 2.1 times that of YK-CAP-101 to 105, respectively, showing a significant increase.
  • YK-CAP-106 to 119 have very high capping rate, all exceeding 95%.
  • YK-CAP-107, YK-CAP-110, YK-CAP-111, YK-CAP-117, and YK-CAP-118 have a capping rate of 97.3%, 97.4%, 98.1%, 95.1%, and 97.2%, respectively, with YK-CAP-111 having the highest capping rate of 98.1%.
  • YK-CAP-102 has the lowest capping rate, which is only 30.2%.
  • YK-CAP-101 and YK-CAP-103 to 105 also have a very low capping rate of 42.8%, 71.1%, 77.2%, and 69.3%, respectively.
  • the capping rate of YK-CAP-111 is 2.3 times, 3.2 times, 1.4 times, 1.3 times, and 1.4 times higher than that of YK-CAP-101 to 105, respectively, showing a significant increase (as shown in FIG. 2 ).
  • the ribose-modified cap analogs of the present disclosure show a significant increase in both in vitro transcription yield of mRNA and capping rate.
  • the transcription yield of YK-CAP-111 is 40.8% higher than that of compound 14, and the capping rate of YK-CAP-111 is 36.4% higher than that of 5227.
  • the in vitro transcription yield of mRNA and capping rate of compound 14 are 123.2 ⁇ g and 78.3%, respectively.
  • the in vitro transcription yield of mRNA and capping rate of YK-CAP-111 in the present disclosure are 40.8% and 25.3% higher than those of compound 14, respectively, showing a significant increase.
  • the in vitro transcription yield of mRNA and capping rate of 5227 are 134.2 ⁇ g and 71.9%, respectively.
  • the in vitro transcription yield of mRNA and capping rate of YK-CAP-111 in the present disclosure are 29.3% and 36.4% higher than those of 5227, respectively, showing a significant increase.
  • ribose-modified cap analogs YK-CAP-117 to 119 designed in the present disclosure have structures that are very similar. This series of compounds is also very similar in structure to 5227, but they vary greatly in the in vitro transcription yield of mRNA and capping rate.
  • compounds YK-CAP-117 to 119 in the present disclosure differ from 5227 only in the substituent at the C4 position of the first sugar ring, i.e., the C4-substituent of 5227 is methoxy; the C4-substituents of YK-CAP-117 to 119 are methoxymethyl, 1-fluoromethyl, and difluoromethyl, respectively.
  • the other structures are exactly identical.
  • the in vitro transcription yield of mRNA of YK-CAP-117 to 119 is 14.2%, 28.3% and 14.9% higher than that of 5227, respectively, and the capping rate of YK-CAP-117 to 119 is 32.3%, 35.2%, and 33.9% higher than that of 5227, respectively, showing a significant increase.
  • ribose-modified cap analogs with similar structures do not necessarily have similar mRNA transcription activities and capping rates. On the contrary, there may be a huge difference.
  • the ribose-modified cap analogs YK-CAP-106 to 119 in the present disclosure show a significant increase in both in vitro transcription yield of mRNA and capping rate compared to both YK-CAP-101 to 105 in the present disclosure and compounds 14 and 5227 in the prior art, which demonstrates that the ribose modification of YK-CAP-106 to 119 shows excellent resistance to reverse transcription during in vitro transcription of mRNA and can greatly enhance the binding ability of the cap structure to the capping enzyme, thereby increasing the capping rate of mRNA transcription.
  • the ethanol lipid solution was mixed with the Fluc-mRNA aqueous solution prepared from different cap structures at a volume ratio of 1:3 using a microfluidic device at a flow rate of 10 mL/min to prepare LNPs at a weight ratio of total lipid to mRNA of approximately 15:1.
  • the resulting liposomes were diluted to 10-fold volume with PBS, and then ultrafiltered with a 300 KDa ultrafiltration tube to remove ethanol. The volume was then fixed to a certain volume with PBS.
  • LNPs were filtered through a 0.2 ⁇ m sterile filter to obtain an LNP preparation encapsulating Fluc-mRNA using YK-009/DSPC/cholesterol/DMG-PEG2000 (at a molar ratio of 49:10:39.5:1.5).
  • the particle size and polydispersity index (PDI) were determined by dynamic light scattering using a Malvern laser particle size analyzer. 10 ⁇ L of the liposome solution was taken, diluted to 1 mL with RNase-free deionized water, and added to a sample pool. Each sample was measured in triplicate. The measurement conditions were: a scattering angle of 90° and a temperature of 25°C. The encapsulation efficiency of LNPs was determined using the Quant-iT RiboGreen RNA Quantification Kit (Thermo Fisher Scientific, UK) according to the manufacturer's instructions.
  • Table 4 Characterization of lipid nanoparticles Name Particle size (nm) PDI EE (%) YK-CAP-101 75.45 0.043 93.4 YK-CAP-102 73.22 0.055 95.3 YK-CAP-103 67.24 0.053 94.7 YK-CAP-104 69.24 0.047 95.7 YK-CAP-105 77.22 0.063 95.7 YK-CAP-106 68.24 0.048 97.1 YK-CAP-107 78.42 0.053 96.3 YK-CAP-108 83.13 0.023 96.4 YK-CAP-109 86.35 0.035 97.2 YK-CAP-110 69.64 0.033 97.7 YK-CAP-111 78.33 0.053 95.1 YK-CAP-112 83.24 0.056 93.5 YK-CAP-113 79.34 0.043 96.1 YK-CAP-101 75.45 0.043 93.4 YK-
  • good lipid nanoparticles can be prepared from the Fluc-mRNA transcribed using the cap analogs YK-CAP-101 to 119 in the present disclosure and the cap analogs 5227, CAP-2'O-ethyl, N-7113, compound 14, HN3002, and m6A disclosed in the prior art. All lipid nanoparticles have a particle size between 66 and 88 nm, a PDI value between 0.023 and 0.078, and an encapsulation efficiency of 90% or more.
  • the relative fluorescence readings of capped mRNAs are shown in Table 5.
  • the relative fluorescence intensity is proportional to the translation efficiency of mRNA.
  • Table 5: Relative fluorescence readings of capped mRNAs Name Relative fluorescence intensity Multiple of m6A YK-CAP-101 0.22 0.6 YK-CAP-102 0.34 0.9 YK-CAP-103 0.77 2.0 YK-CAP-104 0.81 2.1 YK-CAP-105 0.72 1.9 YK-CAP-106 1.53 4.0 YK-CAP-107 1.62 4.3 YK-CAP-108 1.43 3.8 YK-CAP-109 1.53 4.0 YK-CAP-110 1.95 5.1 YK-CAP-111 2.12 5.6 YK-CAP-112 1.74 4.6 YK-CAP-113 1.63 4.3 YK-CAP-114 1.45 3.8 YK-CAP-115 1.69 4.4 YK-CAP-116 1.39 3.7 YK-CAP-
  • YK-CAP-106 to YK-CAP-119 have high relative fluorescence intensity (between 1.4 and 2.2).
  • YK-CAP-107, YK-CAP-110, YK-CAP-111, YK-CAP-117, and YK-CAP-118 have a relative fluorescence intensity of 1.62, 1.95, 2.12, 1.82, and 1.65, respectively, with YK-CAP-111 having the highest relative fluorescence intensity of 2.12, followed by YK-CAP-110 with a relative fluorescence intensity of 1.95.
  • YK-CAP-101 has the lowest relative fluorescence intensity, which is only 0.22.
  • YK-CAP-102 to YK-CAP-105 also have a very low relative fluorescence intensity of 0.34, 0.77, 0.81, and 0.72, respectively.
  • the relative fluorescence intensity of YK-CAP-111 is 9.6 times, 6.2 times, 2.8 times, 2.6 times, and 2.9 times that of YK-CAP-101 to YK-CAP-105, respectively, while the relative fluorescence intensity of YK-CAP-110 is 8.9 times, 5.7 times, 2.5 times, 2.4 times, and 2.7 times that of YK-CAP-101 to YK-CAP-105, respectively (as shown in FIG. 3 ).
  • the ribose-modified cap analogs of the present disclosure show a significant increase in the translation efficiency of mRNA.
  • the translation efficiency of YK-CAP-111 is 5.6 times that of m6A.
  • N-7113, HN3002, compound 14, and m6A have a relative fluorescence intensity (corresponding to the translation efficiency of mRNA) of 1.00, 1.12, 1.13, and 0.38, respectively.
  • the relative fluorescence intensity of YK-CAP-111 in the present disclosure is 2.1 times, 1.9 times, 1.9 times, and 5.6 times that of N-7113, HN3002, compound 14, and m6A, respectively, while the relative fluorescence intensity of YK-CAP-110 in the present disclosure is 1.9 times, 1.7 times, 1.7 times, and 5.1 times that of N-7113, HN3002, compound 14, and m6A, respectively.
  • the ribose-modified cap analogs YK-CAP-117 to 119 designed in the present disclosure have structures that are very similar. This series of compounds is also very similar in structure to 5227, but they vary greatly in the translation efficiency of mRNA.
  • compounds YK-CAP-117 to 119 in the present disclosure differ from 5227 only in the substituent at the C4 position of the first sugar ring, i.e., the C4-substituent of 5227 is methoxy; the C4-substituents of YK-CAP-117 to 119 are methoxymethyl, 1-fluoromethyl, and difluoromethyl, respectively.
  • the other structures are exactly identical.
  • the translation efficiency of mRNA of YK-CAP-117 to 119 is 2.2 times, 2.0 times, and 1.8 times that of 5227, respectively, showing a significant increase.
  • ribose-modified cap analogs YK-CAP-113 to 116 designed in the present disclosure have structures that are very similar. This series of compounds is also very similar in structure to CAP-2'O-ethyl, but they vary greatly in the translation efficiency of mRNA.
  • compounds YK-CAP-113 to 116 in the present disclosure differ from CAP-2'O-ethyl only in the substituent at the C2 position of the second sugar ring, i.e., the C2-substituent of CAP-2'O-ethyl is ethoxy; the C2-substituents of YK-CAP-113 to 116 are methoxymethyl, acetamidomethyl, 1-fluoromethyl, and difluoromethyl, respectively.
  • the other structures are exactly identical.
  • the translation efficiency of mRNA of YK-CAP-113 to 116 is 1.4 times, 1.3 times, 1.5 times, and 1.2 times that of CAP-2'O-ethyl, respectively, showing a significant increase.
  • the ribose-modified cap analogs in the present disclosure show a significant increase in the translation efficiency of mRNA compared to both ribose-modified cap analogs with similar structures (including YK-CAP-101 to 105 in the present disclosure as well as 5227, CAP-2'O-ethyl, N-7113, and HN3002) and ribose-modified cap analogs with vastly different structures (including compound 14 and m6A).
  • ribose-modified cap analogs with similar structures do not necessarily have similar translation efficiency of luciferase mRNA. On the contrary, there may be a huge difference.
  • RNA purified by polyacrylamide gel electrophoresis was subjected to an enzymatic reaction with 50 U of mRNA decapping enzyme (New England Biolabs) and 1 ⁇ MDE buffer at 37°C for 45 minutes.
  • the enzymatic reactants were subjected to PAGE and stained with SYBR Green II (Lonza), followed by observation of the post-electrophoresis gel image on a Typhoon FLA 7000 (GE Healthcare) instrument.
  • the electrophoresis band intensity ratio of RNA capping to RNA decapping was counted using ImageQuant (GE Healthcare) software, and the decapping rate of the decapping enzyme was calculated.
  • Decapping rate after treatment with decapping enzyme Name Decapping rate (%) of DCP2 enzyme Decrease relative to N-7113 (%) YK-CAP-101 32.4 11.4 YK-CAP-102 41.2 2.6 YK-CAP-103 36.5 7.3 YK-CAP-104 46.1 -2.3 YK-CAP-105 33.9 9.9 YK-CAP-106 17.3 26.5 YK-CAP-107 11.5 32.3 YK-CAP-108 16.3 27.5 YK-CAP-109 17.3 26.5 YK-CAP-110 10.2 33.6 YK-CAP-111 11.5 32.3 YK-CAP-112 11.9 31.9 YK-CAP-113 15.3 28.5 YK-CAP-114 14.2 29.6 YK-CAP-115 15.3 28.5 YK-CAP-116 14.3 29.5 YK-CAP-117 9.8 34.0 YK-CAP-118 11.9 31.9 YK-CAP-119 11.4 32.4 5227 23.2 20.6 CAP-2
  • ribose-modified cap analogs YK-CAP-101 to 119 in the present disclosure vary greatly in the decapping rate.
  • YK-CAP-106 to 119 all have very low decapping rates.
  • YK-CAP-107, YK-CAP-110, YK-CAP-111, YK-CAP-117, and YK-CAP-118 have a decapping rate of 11.5%, 10.2%, 11.5%, 9.8%, and 11.9%, respectively, with YK-CAP-117 having the lowest decapping rate of only 9.8%, followed by YK-CAP-110 with a decapping rate of 10.2%.
  • YK-CAP-104 has the highest decapping rate, which is 46.1%.
  • YK-CAP-101, YK-CAP-102, YK-CAP-103, and YK-CAP-105 also have a high decapping rate of 32.4%, 41.2%, 36.5%, and 33.9%, respectively.
  • the decapping rate of YK-CAP-117 is 22.6%, 31.4%, 26.7%, 36.3%, and 24.1% lower than that of YK-CAP-101 to 105, respectively, while the decapping rate of YK-CAP-110 is 22.2%, 31.0%, 26.3%, 35.9%, and 23.7% lower than that of YK-CAP-101 to 105 (as shown in FIG. 4 ).
  • the ribose-modified cap analogs of the present disclosure show a significant decrease in the decapping rate.
  • the decapping rate of YK-CAP-117 is 34.0% lower than that of N-7113.
  • N-7113, compound 14, HN3002, and m6A have a decapping rate of 43.8%, 26.8%, 23.3%, and 33.8%, respectively.
  • the decapping rate of YK-CAP-117 in the present disclosure is 34.0%, 17.0%, 13.5%, and 24.0% lower than that of N-7113, compound 14, HN3002, and m6A, respectively, while the decapping rate of YK-CAP-110 is 33.6%, 16.6%, 13.1%, and 23.6% lower than that of N-7113, compound 14, HN3002, and m6A, respectively.
  • the ribose-modified cap analogs YK-CAP-117 to 119 designed in the present disclosure have structures that are very similar. This series of compounds is also very similar in structure to 5227, but they vary greatly in the mRNA decapping rate.
  • compounds YK-CAP-117 to 119 in the present disclosure differ from 5227 only in the substituent at the C4 position of the first sugar ring, i.e., the C4-substituent of 5227 is methoxy; the C4-substituents of YK-CAP-117 to 119 are methoxymethyl, 1-fluoromethyl, and difluoromethyl, respectively.
  • the other structures are exactly identical.
  • the mRNA decapping rate of YK-CAP-117 to 119 is 13.4%, 11.3%, and 11.8% lower than that of 5227, respectively, showing a significant decrease.
  • the ribose-modified cap analogs YK-CAP-113 to 116 designed in the present disclosure have structures that are very similar. This series of compounds is also very similar in structure to CAP-2'O-ethyl, but they vary greatly in the mRNA decapping rate.
  • compounds YK-CAP-113 to 116 in the present disclosure differ from CAP-2'O-ethyl only in the substituent at the C2 position of the second sugar ring, i.e., the C2-substituent of CAP-2'O-ethyl is ethoxy; the C2-substituents of YK-CAP-113 to 116 are methoxymethyl, acetamidomethyl, 1-fluoromethyl, and difluoromethyl, respectively.
  • the other structures are exactly identical.
  • the mRNA decapping rate of YK-CAP-113 to 116 is 13.0%, 14.1%, 13.0%, and 14.0% lower than that of CAP-2'O-ethyl, respectively, showing a significant decrease.
  • the ribose-modified cap analogs in the present disclosure show a significant decrease in the decapping rate of DCP2 enzyme compared to both ribose-modified cap analogs with similar structures (including YK-CAP-101 to 105 in the present disclosure as well as 5227, CAP-2'O-ethyl, N-7113, and HN3002 in the prior art) and ribose-modified cap analogs with vastly different structures (including compound 14 and m6A).
  • ribose-modified cap analogs with similar structures do not necessarily have similar decapping rates. On the contrary, there may be a huge difference.
  • the LNP preparation containing 5 ⁇ g of cap analog-transcribed Fluc-mRNA was intramuscularly injected into female BALB/C mice aged 4 to 6 weeks and weighing 17 to 19 g. At specific time points after administration (6 hours, 12 hours, 24 hours, 48 hours, 96 hours, and 168 hours), the mice were intraperitoneally injected with fluorescence imaging substrate. The mice were then allowed to move freely for 5 minutes, followed by detection of the total radiation intensity of the protein expressed by LNP-carrying mRNA in the mice using an IVIS Spectrum small-animal in vivo imaging system (corresponding to the amount of protein expression).
  • Table 7 Experimental data of in vivo imaging in mice Name Total radiation intensity ( ⁇ 108 p/s) 6h 12h 24h 48h 96h 168h YK-CAP-101 1.81 0.96 0.36 0.19 0.04 0.02 YK-CAP-106 5.88 6.85 1.76 0.53 0.11 0.03 YK-CAP-107 8.09 6.87 1.92 0.60 0.11 0.03 YK-CAP-108 6.42 6.03 1.70 1.04 0.19 0.02 YK-CAP-109 6.04 5.84 1.85 0.54 0.14 0.03 YK-CAP-110 9.52 10.55 3.04 2.30 0.27 0.04 YK-CAP-111 8.61 9.34 2.75 1.95 0.27 0.03 YK-CAP-112 8.90 7.48 2.44 1.43 0.30 0.03 YK-CAP-113 6.07 7.63 2.02 1.29 0.31 0.04 YK-CAP-114 8.89 5.76 2.31 1.51 0.29 0.03 YK-CAP-115 6.20 5.42 1.85 1.21 0.25 0.02 YK-CAP-116
  • YK-CAP-106 to 119 all have very high total radiation intensity of in vivo imaging in mice, among which YK-CAP-110 has the highest total radiation intensity, reaching 10.55 ⁇ 10 8 p/s at 12 hours and still reaching 2.30 ⁇ 10 8 p/s at 48 hours, while YK-CAP-111 has the second highest total radiation intensity, reaching 9.34 ⁇ 10 8 p/s at 12 hours and still reaching 1.95 ⁇ 10 8 p/s at 48 hours.
  • YK-CAP-101 has the lowest total radiation intensity, which is 0.96 ⁇ 10 8 p/s at 12 hours and only 0.19 ⁇ 10 8 p/s at 48 hours.
  • the total radiation intensity of YK-CAP-110 is 11.0 times that of YK-CAP-101 at 12 hours and 12.1 times that of YK-CAP-101 at 48 hours.
  • the total radiation intensity of YK-CAP-111 is 9.7 times that of YK-CAP-101 at 12 hours and 10.3 times that of YK-CAP-101 at 48 hours.
  • the ribose-modified cap analogs of the present disclosure show a significant increase in the total radiation intensity and duration of the protein expressed by mRNA in mice.
  • the total radiation intensity of YK-CAP-110 is 5.0 times that of m6A at 12 hours and 4.4 times that of m6A at 48 hours.
  • N-7113 and m6A have a total radiation intensity of 4.33 ⁇ 10 8 p/s and 2.12 ⁇ 10 8 p/s at 12 hours, and 0.49 ⁇ 10 8 p/s and 0.52 ⁇ 10 8 p/s at 48 hours.
  • the total radiation intensity of YK-CAP-110 in the present disclosure is 2.4 times that of N-7113 and 5.0 times that of m6A at 12 hours, and 4.7 times that of N-7113 and 4.4 times that of m6A at 48 hours.
  • the total radiation intensity of YK-CAP-111 in the present disclosure is 2.2 times that of N-7113 and 4.4 times that of m6A at 12 hours, and 4.0 times that of N-7113 and 3.8 times that of m6A at 48 hours.
  • the ribose-modified cap analogs YK-CAP-117 to 119 designed in the present disclosure have structures that are very similar. This series of compounds is also very similar in structure to 5227, but they vary greatly in the total radiation intensity of the protein expressed by mRNA in mice.
  • compounds YK-CAP-117 to 119 in the present disclosure differ from 5227 only in the substituent at the C4 position of the first sugar ring, i.e., the C4-substituent of 5227 is methoxy; the C4-substituents of YK-CAP-117 to 119 are methoxymethyl, 1-fluoromethyl, and difluoromethyl, respectively.
  • the other structures are exactly identical.
  • the total radiation intensity of the protein expressed by mRNA in mice of YK-CAP-117 to 119 is 3.8 times, 3.0 times, and 3.1 times that of 5227 at 6 hours, respectively, showing a significant increase.
  • ribose-modified cap analogs YK-CAP-113 to 116 designed in the present disclosure have structures that are very similar. This series of compounds is also very similar in structure to CAP-2'O-ethyl, but they vary greatly in the total radiation intensity of the protein expressed by mRNA in mice.
  • compounds YK-CAP-113 to 116 in the present disclosure differ from CAP-2'O-ethyl only in the substituent at the C2 position of the second sugar ring, i.e., the C2-substituent of CAP-2'O-ethyl is ethoxy; the C2-substituents of YK-CAP-113 to 116 are methoxymethyl, acetamidomethyl, 1-fluoromethyl, and difluoromethyl, respectively.
  • the other structures are exactly identical.
  • the total radiation intensity of the protein expressed by mRNAin mice of YK-CAP-114 and YK-CAP-116 is 1.6 times and 1.7 times that of CAP-2'O-ethyl at 6 hours, respectively, showing a significant increase.
  • Fluc-mRNAs prepared from ribose-modified cap analogs with similar structures do not necessarily have similar amount and duration of protein expression in mice. On the contrary, there may be a huge difference.
  • the ribose-modified cap analogs in the present disclosure show a significant increase in the amount and duration of protein expression by mRNA in mice compared to both ribose-modified cap analogs with similar structures (including YK-CAP-101 to 105 in the present disclosure as well as 5227, N-7113, and CAP-2'O-ethyl in the prior art) and ribose-modified cap analogs with vastly different structures (m6A).
  • the ribose-modified cap analogs YK-CAP-106 to 119 in the present disclosure show a significant increase in the in vitro transcription yield of mRNA, capping rate, translation efficiency of mRNA, decapping enzyme stability, and amount and duration of protein expression in animals compared to the ribose-modified cap analogs in the prior art (including 5227, CAP-2'O-ethyl, N-7113, compound 14, HN3002, and m6A).
  • YK-CAP-106 to 119 cap structures can significantly enhance the resistance of ribose-modified structures to decapping enzymes as well as their binding affinity to capping enzymes, providing a novel and efficient ribose-modified cap structure for in vitro transcription of mRNA.
  • the present disclosure illustrates the ribose-modified cap analogs of the present disclosure and the use thereof through the above examples.
  • the present disclosure is not limited to these examples, which does not mean that the present disclosure must be implemented depending on these examples. It should be understood by those skilled in the art that any improvements to the present disclosure, equivalent substitutions of starting materials for the products of the present disclosure, additions of auxiliary ingredients, selections of specific means, etc., all fall within the scope of protection and disclosure of the present disclosure.

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