[go: up one dir, main page]

EP4430383A1 - Appareil optique pour exciter un échantillon, analyseur et procédé pour exciter un échantillon - Google Patents

Appareil optique pour exciter un échantillon, analyseur et procédé pour exciter un échantillon

Info

Publication number
EP4430383A1
EP4430383A1 EP22803332.0A EP22803332A EP4430383A1 EP 4430383 A1 EP4430383 A1 EP 4430383A1 EP 22803332 A EP22803332 A EP 22803332A EP 4430383 A1 EP4430383 A1 EP 4430383A1
Authority
EP
European Patent Office
Prior art keywords
area
light
projection
wavelength
hologram
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP22803332.0A
Other languages
German (de)
English (en)
Inventor
Reinhold Fiess
Ingo Ramsteiner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP4430383A1 publication Critical patent/EP4430383A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B5/00—Optical elements other than lenses
    • G02B5/32—Holograms used as optical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/168—Specific optical properties, e.g. reflective coatings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7756—Sensor type
    • G01N2021/7759—Dipstick; Test strip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/107—CRT flying spot scan

Definitions

  • Optical device for exciting a sample analyzer and method for exciting a sample
  • the invention is based on an optical device for exciting a sample, an analysis device and a method for exciting a sample according to the species of the independent claims.
  • lab-on-chip cartridges containing a sample can be entered into analysis devices and processed.
  • a molecular diagnostic assay can be arranged on a plastic cartridge with a microfluidic network.
  • the analysis device can be designed to process such cartridges, ie it can control microfluidic processes on the cartridge and, for example, heat or illuminate specific areas.
  • the optical device presented here advantageously enables efficient projection of light to a surface to be illuminated with variable lighting options.
  • the device can be implemented in a compact design and in a cost-saving manner, in particular with regard to the long service life.
  • An optical device for exciting a sample in a microfluidic device arranged in a receiving area of an analysis device having a light source which is designed to emit a light beam.
  • the optical device comprises a holographic optical element for redirecting at least part of the light beam onto a projection surface of the microfluidic device in order to excite the sample arranged in the microfluidic device when the microfluidic device is arranged in the receiving area of the analysis device.
  • the holographic optical element comprises at least a first hologram area and a second hologram area, the first hologram area being designed to redirect light of a first wavelength of the light beam in a first beam direction onto a first projection area of the projection surface and the second hologram area being designed to direct light of a to redirect the second wavelength of the light beam in a second beam direction to the first projection area and additionally or alternatively to a second projection area of the projection surface.
  • the analysis device can be a device for carrying out diagnostic tests, such as rapid PCR tests.
  • a sample which can be a liquid with sample material or a solid sample, can be entered, for example, into a suitable microfluidic device, which can be a lab-on-chip cartridge with a microfluidic network, for example can act to process the sample.
  • the microfluidic device with the sample can, for example, be entered manually into the receiving area of the analysis device in order to be processed within the analysis device.
  • the analysis device can include the optical device described here, which can also be referred to as an optical unit, in order to excite the sample by illuminating it.
  • the light source used in the device for example, a or have multiple phosphor-converted laser diodes.
  • the light source can include LEDs.
  • the illumination distribution can be controlled, for example, by means of a light projector, such as a so-called flying spot projector, in that the light beam can be directed, for example, with the aid of a micromechanical mirror.
  • the light is not directed directly onto the surface to be illuminated, the lab-on-chip cartridge, but indirectly via the holographic optical element (HOE), which selects the wavelength.
  • the holographic optical element can have a surface made up of several areas. Each of these hologram areas can include a hologram, preferably a volume hologram, which redirects a light beam coming from the projector to a specific point on the projection surface.
  • the first wavelength assigned to the first hologram area can differ from the second wavelength. Accordingly, the first beam direction can differ from the second beam direction.
  • the first wavelength can also stand for a first wavelength range and the second wavelength correspondingly for a second wavelength range. Accordingly, a feature of the HOE is that the individual surfaces can be effective for different wavelengths.
  • the optical device is thus advantageously designed to illuminate specific areas of the cartridge with light of a defined wavelength range, for example in order to stimulate fluorescence there.
  • the holographic optical element can have any suitable number of hologram areas in order to redirect light of any suitable number of different wavelengths onto a suitable number of different projection areas.
  • the holographic optical element can have at least one group with any suitable number of hologram areas in order to redirect light of different wavelengths onto the same projection area assigned to the group.
  • Each hologram area of the group can be assigned a wavelength that differs from the wavelengths of the other hologram areas of the group. If the holographic optical If the element has a plurality of such mutually corresponding groups, each of the groups can be designed to redirect light to a different projection area assigned to the corresponding group.
  • each group can be assigned its own projection area, which can differ from the projection areas of the other groups.
  • the optical device can have a steering device, which can be designed to direct the light beam onto one of the hologram areas in response to a steering signal.
  • the steering device can include a micromechanical mirror, for example, which can be set at specific angles using the steering signal, for example, in order to thereby direct the light beam to a desired hologram area.
  • the hologram areas can be controlled sequentially, for example first the first hologram area and then the second hologram area, with the light beam deflected by the hologram areas being able to be used, for example, to excite the same sample or to excite two different samples.
  • the precise steering of the light beam can optimize an analysis process and save energy at the same time.
  • the optical device can have a control device, which can be designed to provide the steering signal and additionally or alternatively to provide an action signal for switching the light source on and off.
  • the control device can be designed to control a large number of analysis processes and thereby advantageously optimize the implementation of an analysis process.
  • the control device can be designed, for example, to use an identification signal to identify insertion of the microfluidic device and additionally or alternatively the type of microfluidic device used and to provide the action signal and the steering signal accordingly, for example.
  • control device can have at least one computing unit for processing signals or data, at least one memory unit for storing signals or data, at least one interface to a sensor or an actuator for reading in sensor signals from the sensor or for outputting data or control signals to the Have actuator and / or at least one communication interface for reading or outputting data that are embedded in a communication protocol.
  • the arithmetic unit can be, for example, a signal processor, a microcontroller or the like, with the memory unit being able to be a flash memory, an EEPROM or a magnetic memory unit.
  • the communication interface can be designed to read in or output data wirelessly and/or by wire, wherein a communication interface that can read in or output wire-bound data can, for example, read this data electrically or optically from a corresponding data transmission line or can output it to a corresponding data transmission line.
  • a control device can be understood to mean an electrical device that processes sensor signals and, depending thereon, outputs control and/or data signals.
  • the control device can have an interface that can be configured as hardware and/or software.
  • the interfaces can be part of a so-called system ASIC, for example, which contains a wide variety of functions of the device.
  • the interfaces can be separate integrated circuits or to consist at least partially of discrete components.
  • the interfaces can be software modules which are present, for example, on a microcontroller alongside other software modules.
  • the light source can be designed to emit the light beam with at least one broad wavelength band and additionally or alternatively with a plurality of wavelength lines or wavelength bands spaced apart from one another.
  • the optical device, or a projector used in the optical device can be based on a white light source, for example on a phosphor-converted laser or a phosphor mix with several narrow emission bands, which can also be referred to as sharp wavelength lines.
  • the term "white” is not necessarily to be understood as a color impression, but in such a way that the spectrum cannot consist of a single, narrow wavelength band, but, for example, of one or more broad bands or at least several sharp lines.
  • the light beam emitted by the light source can thus advantageously contain all wavelengths that are to be used for illuminating the projection surface.
  • the holographically optical element can comprise at least one further hologram area, which can be designed to project light of a further wavelength of the light beam in a further beam direction onto the first projection area and additionally or alternatively the second projection area and additionally or alternatively a further projection area of the redirect projection screen.
  • the holographic optical element can be formed with any number of hologram areas that can redirect light of a corresponding number of different wavelength ranges.
  • each hologram area can be used to redirect light that differs in wavelength from light with which the other of the hologram areas are irradiated.
  • between 4 and 6 corresponding hologram areas can be provided.
  • the light beam can advantageously be reduced to a wavelength that is optimal for analyzing the sample, and the direction of the beam can be directed as precisely as possible to the projection area on which the sample is arranged.
  • the holographic optical element can be designed to transmit at least another part of the light beam.
  • each hologram area can be designed with a specific intrinsic wavelength selectivity, as a result of which only light of a specific wavelength can be deflected.
  • the remaining light components which can be irrelevant for the analysis process carried out, for example, can be caused by the holographic optical element, for example pass through and sink behind it in a beam trap or be absorbed on a suitable surface.
  • each sample can be illuminated using only a predetermined wavelength.
  • the device can be designed to be able to illuminate the projection surface without gaps.
  • the holographic optical element can be formed with a multiplicity of hologram areas, each hologram area being able to deflect a specific part of the light beam onto a projection area that is arranged differently from one another.
  • the individual projection areas can directly adjoin one another. This has the advantage that a sample can be optimally illuminated independently of its arrangement on the projection surface.
  • the device can be designed to illuminate the first projection area and the second projection area with the same intensity.
  • a projection grid can be matched to the intrinsic beam divergence, or the individual holograms can be designed in such a way that they not only deflect the light beam, but can also shape the wavefront of the deflected light beam. This has the advantage that each sample can be optimally excited.
  • the holographically optical element can comprise at least one additional first hologram area, wherein the additional first hologram area can be designed to direct light of the first wavelength of the light beam in an additional beam direction onto the first projection area and additionally or alternatively onto the second projection area of the projection surface to redirect.
  • the holographic optical element can have a number of hologram areas which, for example, can illuminate identical points on the projection area with the same type of wavelength. This advantageously makes it possible to vary the direction of illumination. This can be used, for example, in connection with inhomogeneous samples, in which the fluorescence, for example, not only depends on the intensity, but also on the direction of incidence of the Excitation light can be dependent.
  • the first and the second projection area can be exposed with the same wavelength, for example in order to excite two identical samples at the same time.
  • the holographically optical element can be shaped flatly and additionally or alternatively the hologram areas of the holographically optical element can be arranged in a matrix-like manner.
  • the holographic optical element can be optimized to the requirements of the analysis process both in terms of shape and in terms of the number and arrangement of the hologram areas.
  • the number of hologram areas that are different in relation to the wavelengths to be diverted and the total number of hologram areas as well as their arrangement can be suitably selected.
  • an analysis device for analyzing a sample in a microfluidic device comprising a receiving area for receiving the microfluidic device and a variant of the optical device presented above.
  • a method for exciting a sample in a microfluidic device arranged in a receiving area of an analysis device comprising a step of emitting a light beam.
  • the method includes a step of redirecting light of at least a first wavelength of the light beam in a first beam direction onto a first projection area of a projection surface of the microfluidic device in order to excite the sample arranged in the first projection area when the microfluidic device is arranged in the receiving area.
  • the method comprises a step of redirecting light of at least a second wavelength of the light beam in a second beam direction onto the first projection area and additionally or alternatively a second projection area of the projection surface of the microfluidic device in order to excite the sample arranged in the first projection area, if the microfluidic device is arranged in the receiving area.
  • FIG. 1 shows a schematic representation of an exemplary embodiment of an analysis device
  • FIG. 2 shows a schematic representation of an optical device according to an embodiment
  • FIG. 3 shows a schematic representation of an exemplary embodiment of an optical device
  • FIG. 4 shows a schematic representation of an exemplary embodiment of an optical device
  • FIG. 5 shows a flow chart of a method for exciting a sample according to an embodiment.
  • the analysis device 100 is designed to analyze entered samples, as a result of which, for example, PCR tests can be carried out.
  • a microfluidic device 105 which is merely an example of a cartridge with a plastic housing and a microfluidic network for processing the sample, can be introduced into a receiving area 110.
  • the analysis device also includes a display 115 with a touch function, by means of which settings for the desired analysis process can be made manually, merely by way of example can be entered.
  • the display 115 is designed only by way of example to display analysis results.
  • the concept of the analyzer envisages the integration of a molecular diagnostic assay on a plastic cartridge with a microfluidic network.
  • the actual device is designed to process such cartridges, i.e. it can control microfluidic processes on the cartridge and heat certain areas and additionally or alternatively illuminate them.
  • it comprises an optical device, as is described in more detail in the following FIGS. 2, 4 and 5, which can excite and evaluate fluorescence signals.
  • this unit consists of two parts: First, a camera with changeable bandpass filters that views a specific area of the cartridge. Secondly, from a device that can illuminate certain areas of the cartridge with light of a defined wavelength range in order to stimulate fluorescence there. These areas are arranged in the field of view of the camera.
  • the optical device 200 can be used in an analysis device, as was described in the previous figure, in order to excite a sample 202 arranged in a microfluidic device 105 by means of illumination.
  • the optical device 200 includes a light source 205, which is designed to emit a light beam.
  • the light source 205 is configured as a projector, merely by way of example, in which case the light beam X can be output at a variable angle. Two different output directions of the light beam X are shown by way of example in FIG.
  • the light source 205 is further configured to output the light beam X having a plurality of wide wavelength bands.
  • the light beam may be output with only a wide wavelength band or with a plurality of sharp wavelength lines.
  • the light beam can be directed onto a holographic optical element 215 within the optical device 200 .
  • the holographic optical element 215, or the HOE for short, is designed to redirect a portion 220 of the light beam X onto a projection surface 225 of the microfluidic device 105 in order to excite the sample 202 arranged in the microfluidic device 105 .
  • the holographic optical element 215 comprises a first hologram area 230, which is designed to redirect light of a first wavelength or a first wavelength range of the light beam X in a first beam direction 235 onto a first projection area 240 of the projection surface 225, on which, in this exemplary embodiment, the Sample 202 is placed.
  • the holographic optical element 215 comprises a second hologram area 250, which is designed to redirect light of a second wavelength or a second wavelength range of the light beam X in a second beam direction 255 onto a second projection area 260 of the projection surface 225.
  • the second projection area 260 in this exemplary embodiment can be illuminated with the same intensity as the first projection area 240, purely by way of example.
  • the holographic optical element 215 is formed flatly with a plurality of further hologram areas, with the hologram areas being arranged in a matrix-like manner merely by way of example.
  • the projection surface 225 can be illuminated without gaps by means of the steerable light beam X and the multiplicity of hologram regions
  • the optical device 200 is designed to control an illumination distribution by means of a light projector.
  • the light beam X can only be steered by means of a flying spot projector with the aid of a micromechanical mirror.
  • the projector is based on a white light source 205, which is a phosphor-converted laser based example only.
  • white is not necessarily to be understood here as a color impression, but in such a way that the spectrum does not consist of a single, narrow wavelength band, but rather one or more broad bands or at least several sharp lines. It is important that it contains all wavelengths that Illumination of the projection surface 225 are to be used.
  • the light cannot be directed directly onto the surface to be illuminated (the lab-on-chip cartridge), but indirectly via a holographic optical element 215, which carries out a wavelength selection.
  • the holographic optical element 215 has a surface made up of a plurality of regions, merely by way of example. Each area includes a hologram, which in this exemplary embodiment is a volume hologram that directs the light beam to a specific point on the projection surface 225 .
  • a hologram which in this exemplary embodiment is a volume hologram that directs the light beam to a specific point on the projection surface 225 .
  • only light around a specific wavelength is appropriately deflected so as to excite the sample 202.
  • FIG. 3 shows a schematic illustration of an exemplary embodiment of an optical device 200.
  • the optical device 200 illustrated here corresponds to or is similar to the device described in FIG. 2 above.
  • the light beam X can be provided by a so-called flying spot projector 400, which is designed to direct a white beam over a specific angular range and also to switch it on and off during the scanning process in order to only select selected positions in its projection range to illuminate.
  • the flying spot projector 400 comprises, merely by way of example, the light source 205 and a steering device 405, which in this exemplary embodiment comprises a micromechanically movable mirror and is designed to direct the light beam X onto one of the hologram areas of the holographic optical element 215 in response to a steering signal 410 to steer.
  • the steering signal 410 can only be provided by a control device 415 as an example.
  • the control device 415 is also designed to provide an action signal 420 for switching the light source 205 on and off in addition to the steering signal 410 .
  • the light beam X can be guided by means of the steering device 405 onto a first hologram area 230 and a second hologram area 250 of the holographic optical element 215 .
  • the holographically optical element 215 additionally comprises a further hologram area 430 and only by way of example an additional hologram area 440, the hologram areas 230, 250, 430, 440 being arranged in a matrix-like manner merely by way of example.
  • First hologram area 230 is designed to redirect light of a first wavelength AA of light beam ⁇ in a first beam direction to a first projection area of projection surface 225
  • second hologram area 250 is designed to redirect light of a second wavelength AB of light beam A in a second beam direction to a second projection area of the projection surface 225
  • additional hologram area 430 is designed to redirect light of an additional wavelength Ac of light beam A in an additional beam direction to an additional projection area of projection surface 225
  • additional hologram area 440 is designed to redirect light of an additional wavelength AD of light beam A in to redirect an additional beam direction to an additional projection area of the projection surface 225 .
  • each individual hologram of the HOE thus directs a wavelength-specific part of the light beam A to a specific point on the projection surface 225, with the areas of the HOE shown labeled A, B, C and D only emitting light with the wavelengths AA, AB, AC, Process AD.
  • the light of the wavelengths AA, AB, AC, AD thus represents different portions of the light beam A, which are selected and deflected by the hologram areas 230, 250, 430, 440.
  • the spectra shown represent examples of the respective beams.
  • the beams traveling from the projector to the HOE have a broad spectrum, from which the A range deflects a narrow wavelength band with the central wavelength AA, just by way of example, and the B range deflects a narrow wavelength band with AB.
  • the holographic optical element 215 is designed to redirect four wavelengths XA, AB, XC, XD with a plurality of hologram areas.
  • the concept is not limited to four wavelengths, and the regions of the HOE may have a different shape than the square shown here, or different sizes, or follow a different symmetry in their arrangement.
  • the optical device 200 shown here enables one or more connected surfaces, for example on a lab-on-chip cartridge, to be illuminated with light.
  • the number, shape and extent of the surfaces can be freely selected within a certain range and flexibly controlled electronically.
  • the light itself meets the requirements for the fluorescence excitation of molecular diagnostic assays and also includes a number of color channels for this purpose. This means a spectrum that is precisely defined in terms of central wavelength and width, or several such spectra between which it is possible to switch.
  • the embodiment described in this exemplary embodiment with a white-light flying spot projector offers the advantage of particular light efficiency.
  • other projector types DLP, LCOS
  • the image plane of the projector should be in the area of the HOE, in other words, the image of the projector should be focused on the HOE.
  • FIG. 4 shows a schematic illustration of an exemplary embodiment of an optical device 200.
  • the optical device 200 illustrated here corresponds to or is similar to the device described in FIGS. 2 and 3 above.
  • the holographic optical element 215 in this exemplary embodiment comprises an additional first hologram area 500, merely by way of example Projection area 260 of Redirect projection surface, while the first hologram area 230 is formed to redirect light onto the first projection area 240.
  • the first hologram area 230 in this exemplary embodiment is embodied as part of a first group 510 which, for example, includes four HOE areas with different wavelengths that overlap in a common projection area, which corresponds to the first projection area 240 in this exemplary embodiment.
  • the additional first hologram area 500 is formed, merely by way of example, as part of a second group 515, which also includes, by way of example, four HOE areas with different wavelengths that overlap in a common projection area, which in this exemplary embodiment corresponds to the second projection area 260 .
  • the projection surface can be covered without gaps with each wavelength channel.
  • an intensity distribution that is as equal as possible can be achieved with each channel, merely by way of example.
  • the projection grid can alternatively be matched to the intrinsic beam divergence.
  • the additional first hologram area can be designed to redirect light of the first wavelength of the light beam in an additional beam direction onto the first projection area of the projection surface.
  • multiple elements of the same wavelength type can illuminate identical points on the projection area. This can make it possible to vary the direction of illumination. This can be used in connection with inhomogeneous samples, where the fluorescence depends not only on the intensity but also on the direction of incidence of the excitation light.
  • FIG. 5 shows a flow chart of a method 700 for exciting a sample according to an embodiment.
  • the sample is in one in one Recording area of an analysis device arranged microfluidic device arranged.
  • the method 700 includes a step 705 of outputting a light beam. This is followed by a step 710 of redirecting, in which a first portion of the light beam, which has a first wavelength, is redirected in a first beam direction onto a first projection area of a projection surface of the microfluidic device in order to excite the sample arranged in the first projection area.
  • the method 700 in this exemplary embodiment includes a step 715 of redirecting in which a second portion of the light beam, which has a second wavelength, in a second beam direction onto a second projection area of the
  • Projection area of the microfluidic device is diverted to excite the sample arranged in the first projection area.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Molecular Biology (AREA)
  • Holo Graphy (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un appareil optique (200) pour exciter un échantillon (202) dans un dispositif microfluidique (105) disposé dans une région de réception d'un analyseur, ledit appareil comprenant une source de lumière (205) pour émettre un faisceau lumineux (λ) et un élément optique holographique (215) pour dévier au moins une partie (220) du faisceau lumineux (λ) sur une surface de projection (225) du dispositif microfluidique (105) afin d'exciter l'échantillon (202) disposé dans le dispositif microfluidique (105) lorsque le dispositif microfluidique (105) est disposé dans la région de réception de l'analyseur. L'élément optique holographique (215) comprend des régions d'hologramme (230, 250), qui sont conçues pour dévier la lumière de différentes longueurs d'onde (λA, λB) du faisceau lumineux (λ) dans différentes directions de faisceau sur une zone de projection (260) de la surface de projection (225).
EP22803332.0A 2021-11-08 2022-10-31 Appareil optique pour exciter un échantillon, analyseur et procédé pour exciter un échantillon Pending EP4430383A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102021212505.0A DE102021212505A1 (de) 2021-11-08 2021-11-08 Optische Vorrichtung zum Anregen einer Probe, Analysegerät und Verfahren zum Anregen einer Probe
PCT/EP2022/080324 WO2023078817A1 (fr) 2021-11-08 2022-10-31 Appareil optique pour exciter un échantillon, analyseur et procédé pour exciter un échantillon

Publications (1)

Publication Number Publication Date
EP4430383A1 true EP4430383A1 (fr) 2024-09-18

Family

ID=84359324

Family Applications (1)

Application Number Title Priority Date Filing Date
EP22803332.0A Pending EP4430383A1 (fr) 2021-11-08 2022-10-31 Appareil optique pour exciter un échantillon, analyseur et procédé pour exciter un échantillon

Country Status (5)

Country Link
US (1) US20240418922A1 (fr)
EP (1) EP4430383A1 (fr)
CN (1) CN118202230A (fr)
DE (1) DE102021212505A1 (fr)
WO (1) WO2023078817A1 (fr)

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19628562A1 (de) * 1996-07-16 1998-01-22 Boehringer Mannheim Gmbh Analysesystem mit Mitteln zur Erkennung von Unterdosierungen
PL341387A1 (en) 1997-12-29 2001-04-09 Novartis Ag Multiple-focus holographic lens
DE19819537A1 (de) 1998-04-30 2000-03-16 Biochip Technologies Gmbh Analyse- und Diagnostikinstrument
US6356366B1 (en) 1999-10-21 2002-03-12 Digilens, Inc. Holographic light focusing device
US6863406B2 (en) 2002-08-01 2005-03-08 The University Of Chicago Apparatus and method for fabricating, sorting, and integrating materials with holographic optical traps
CN100475317C (zh) 2003-09-04 2009-04-08 阿尔利克斯公司 利用激光操纵进行基于多层流的颗粒和细胞分离
KR100590548B1 (ko) 2004-03-03 2006-06-19 삼성전자주식회사 광검출 장치
CN1973413A (zh) 2004-03-17 2007-05-30 阿尔利克斯公司 使用全息光学捕获来操控和处理材料的系统和方法
EP2419724A4 (fr) * 2009-04-15 2012-11-28 Relia Diagnostic Systems Inc Extension de la plage dynamique d'une bandelette réactive
WO2011008233A1 (fr) * 2009-05-07 2011-01-20 President And Fellows Of Harvard College Procédés et appareils de détection fluorimétrique employant des plaques à zone de fresnel
US8767216B2 (en) 2009-10-13 2014-07-01 California Institute Of Technology Holographically illuminated imaging devices
US9357202B2 (en) 2010-02-23 2016-05-31 California Institute Of Technology High resolution imaging devices with wide field and extended focus
EP2602608B1 (fr) 2011-12-07 2016-09-14 Imec Analyse et tri des cellules biologiques dans un écoulement
CN102636471A (zh) 2012-04-26 2012-08-15 大连理工大学 基于光子晶体谐振腔led激发光源的微流控芯片荧光检测系统
CN107850537B (zh) 2015-06-30 2020-05-19 Imec 非营利协会 辐射载体及其在光学传感器中的使用
EP3339834B1 (fr) 2016-12-22 2021-11-10 IMEC vzw Cytomètre en flux à conception de crête d'intensité multiple
CN111808737A (zh) 2019-04-12 2020-10-23 牛蓉 一种细胞分选系统
CN109943475A (zh) 2019-04-12 2019-06-28 广西医科大学第一附属医院 一种微流控分选芯片及其分选系统
DE102020201112A1 (de) * 2020-01-30 2021-08-05 Robert Bosch Gesellschaft mit beschränkter Haftung Optische Auslesung von Prozessen innerhalb einer mikrofluidischen Vorrichtung
WO2021230868A1 (fr) 2020-05-14 2021-11-18 Hewlett-Packard Development Company, L.P. Capteur d'azote-lacune à optique intégrée
CN112779156B (zh) 2020-12-29 2022-10-25 西安交通大学 一种基于空间光调制技术的纳秒脉冲激光穿孔系统和方法
EP4314775A1 (fr) * 2021-03-23 2024-02-07 Robert Bosch GmbH Appareil, dispositif et procédé d'irradiation d'un échantillon biologique particulier, avec composant optique holographique
CN114433262B (zh) 2022-01-26 2023-08-22 合肥工业大学 一种多粒子快速捕获系统及其操作方法

Also Published As

Publication number Publication date
US20240418922A1 (en) 2024-12-19
WO2023078817A1 (fr) 2023-05-11
DE102021212505A1 (de) 2023-05-11
CN118202230A (zh) 2024-06-14

Similar Documents

Publication Publication Date Title
DE69115701T2 (de) Konfokales optisches rastermikroskop
DE69629794T2 (de) Kompakter abtastkopf mit mehrfachen abtastmodalitaeten
DE19829944C2 (de) Verfahren und Anordnung zur Gerätekonfiguration eines Fluoreszenz-Laserscanmikroskops
EP1949082B1 (fr) Dispositif d éclairage multispectral
EP0898783B1 (fr) Microscope a balayage dans lequel un echantillon est excite optiquement simultanement en plusieurs points
EP3033646B1 (fr) Microscopie à balayage haute résolution
EP3217205B1 (fr) Dispositif et procédé de microscopie par balayage multizone
EP2148187A1 (fr) Optique d'excitation et de représentation pour la détection de fluorescence
EP0114053A2 (fr) Dispositif de détermination de la direction d'incidence d'un rayonnement optique
EP1894051A2 (fr) Microscope
EP3271694A1 (fr) Appareil de mesure d'émission de lumière et procédé pour la mesure d'une d'émission de lumière
DE112015006100T5 (de) Mehrfarbenerfassungsvorrichtung
EP1341023A2 (fr) Méthode et software d'apprentissage pour microscope de balayage et microscope de balayage
DE69203195T3 (de) Vorrichtung zur Spektralphotometrie mit Spektralbandfilterung.
EP1542051B1 (fr) Procédé et système pour séparer des longueurs d'onde dans un microscope à balayage
DE102013227103A1 (de) Mikroskop mit einer akustooptischen Vorrichtung
EP4430383A1 (fr) Appareil optique pour exciter un échantillon, analyseur et procédé pour exciter un échantillon
DE10317669B4 (de) Verfahren zur Separierung von Detektionskanälen eines mikroskopischen Systems
WO2023180099A1 (fr) Appareil d'éclairage pour éclairage d'un dispositif microfluidique, analyseur comportant un appareil d'éclairage et procédé d'éclairage d'un dispositif microfluidique
EP1023622B1 (fr) Dispositif pour le melange et/ou la repartition, en fonction de la longueur d'onde, de lumiere polychromatique
DE102022200502A1 (de) Beleuchtungsvorrichtung zum Beleuchten einer mikrofluidischen Einrichtung, Analysegerät mit Beleuchtungsvorrichtung und Verfahren zum Beleuchten einer mikrofluidischen Einrichtung
WO2002014811A1 (fr) Unite de detection de lumiere et procede de detection de lumiere luminescente, et microscope confocal pour microscopie par luminescence
DE102022202634A1 (de) Vorrichtung, Einrichtung und Verfahren zur Bestrahlung einer insbesondere biologischen Probe mit einem holografisch-optischen Bauelement
DE102023206988A1 (de) Vorrichtung zum Bereitstellen von Anregungslicht, Verfahren zum Beleuchten einer diagnostischen Probe und Verfahren zum Analysieren einer diagnostischen Probe
WO2025252958A1 (fr) Dispositif optique pour diriger un faisceau lumineux et procédé de fonctionnement d'un dispositif optique

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: UNKNOWN

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20240610

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)