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EP4363612A4 - Procédé de détection et de quantification de la méthylation de l'adn dans un locus ou une région d'adn choisi - Google Patents

Procédé de détection et de quantification de la méthylation de l'adn dans un locus ou une région d'adn choisi

Info

Publication number
EP4363612A4
EP4363612A4 EP22834120.2A EP22834120A EP4363612A4 EP 4363612 A4 EP4363612 A4 EP 4363612A4 EP 22834120 A EP22834120 A EP 22834120A EP 4363612 A4 EP4363612 A4 EP 4363612A4
Authority
EP
European Patent Office
Prior art keywords
dna
detecting
region
quantifying
selected locus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP22834120.2A
Other languages
German (de)
English (en)
Other versions
EP4363612A1 (fr
Inventor
Elazar Rabbani
Jack Coleman
Maurizio Mauro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Biochem Inc
Original Assignee
Enzo Biochem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc
Publication of EP4363612A1 publication Critical patent/EP4363612A1/fr
Publication of EP4363612A4 publication Critical patent/EP4363612A4/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP22834120.2A 2021-07-02 2022-06-29 Procédé de détection et de quantification de la méthylation de l'adn dans un locus ou une région d'adn choisi Pending EP4363612A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163218008P 2021-07-02 2021-07-02
PCT/US2022/035455 WO2023278528A1 (fr) 2021-07-02 2022-06-29 Procédé de détection et de quantification de la méthylation de l'adn dans un locus ou une région d'adn choisi

Publications (2)

Publication Number Publication Date
EP4363612A1 EP4363612A1 (fr) 2024-05-08
EP4363612A4 true EP4363612A4 (fr) 2025-06-11

Family

ID=84690599

Family Applications (1)

Application Number Title Priority Date Filing Date
EP22834120.2A Pending EP4363612A4 (fr) 2021-07-02 2022-06-29 Procédé de détection et de quantification de la méthylation de l'adn dans un locus ou une région d'adn choisi

Country Status (4)

Country Link
EP (1) EP4363612A4 (fr)
JP (1) JP2024526281A (fr)
CA (1) CA3224437A1 (fr)
WO (1) WO2023278528A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060194208A1 (en) * 2003-08-15 2006-08-31 Reimo Tetzner Method for the detection of cytosine methylations in dna with the aid of scorpion
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
US20200332348A1 (en) * 2018-01-31 2020-10-22 Enzo Biochem, Inc. Nucleic acid probes for in situ hybridization

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9261460B2 (en) * 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
JP2009247260A (ja) * 2008-04-04 2009-10-29 Sumitomo Chemical Co Ltd Ｄｎａメチル化測定方法
WO2011127136A1 (fr) * 2010-04-06 2011-10-13 University Of Chicago Compositions et procédés liés à la modification de 5-hydroxyméthylcytosine (5-hmc)
AU2013266341B2 (en) * 2012-05-22 2019-01-03 The Johns Hopkins University A quantitative multiplex methylation specific PCR method- cMethDNA, reagents, and its use
US20170204481A1 (en) * 2014-12-22 2017-07-20 Enzo Biochem, Inc. Comprehensive and comparative flow cytometry-based methods for identifying the state of a biological system
IL319255A (en) * 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
CN114041044A (zh) * 2019-07-01 2022-02-11 达芬奇生物有限公司 高容量分子检测
US20220403471A1 (en) * 2019-08-27 2022-12-22 Exact Sciences Corporation Characterizing methylated dna, rna, and proteins in subjects suspected of having lung neoplasia

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060194208A1 (en) * 2003-08-15 2006-08-31 Reimo Tetzner Method for the detection of cytosine methylations in dna with the aid of scorpion
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
US20200332348A1 (en) * 2018-01-31 2020-10-22 Enzo Biochem, Inc. Nucleic acid probes for in situ hybridization

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KOO DAL-HOE ET AL: "Super-stretched pachytene chromosomes for fluorescence in situ hybridization mapping and immunodetection of DNA methylation", THE PLANT JOURNAL, vol. 59, no. 3, 24 July 2009 (2009-07-24), GB, pages 509 - 516, XP093270488, ISSN: 0960-7412, DOI: 10.1111/j.1365-313X.2009.03881.x *
MAURO MAURIZIO ET AL: "RECENT IMPROVEMENTS IN IN SITU HYBRIDIZATION FOR THE DETECTION OF HPV INFECTIONS IN CLINICAL SAMPLES", 14 July 2020 (2020-07-14), XP093269900, Retrieved from the Internet <URL:https://www.wcrj.net/wp-content/uploads/sites/5/2020/03/e1542-Recent-improvements-in-in-situ-hybridization-for-the-detection-of-HPV-infections-in-clinical-samples-1.pdf> *
See also references of WO2023278528A1 *

Also Published As

Publication number Publication date
EP4363612A1 (fr) 2024-05-08
JP2024526281A (ja) 2024-07-17
CA3224437A1 (fr) 2023-01-05
WO2023278528A1 (fr) 2023-01-05

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A4 Supplementary search report drawn up and despatched

Effective date: 20250512

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 33/52 20060101ALI20250506BHEP

Ipc: C12Q 1/682 20180101AFI20250506BHEP