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EP4225911A4 - Plateforme nanoporeuse prête à l'emploi pour la détection d'adn/arn attomole à l'aide d'une sonde complémentaire marquée à l'osmium - Google Patents

Plateforme nanoporeuse prête à l'emploi pour la détection d'adn/arn attomole à l'aide d'une sonde complémentaire marquée à l'osmium Download PDF

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Publication number
EP4225911A4
EP4225911A4 EP20956871.6A EP20956871A EP4225911A4 EP 4225911 A4 EP4225911 A4 EP 4225911A4 EP 20956871 A EP20956871 A EP 20956871A EP 4225911 A4 EP4225911 A4 EP 4225911A4
Authority
EP
European Patent Office
Prior art keywords
attomol
osmium
dna
ready
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20956871.6A
Other languages
German (de)
English (en)
Other versions
EP4225911A1 (fr
Inventor
Anastassia Kanavarioti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP4225911A1 publication Critical patent/EP4225911A1/fr
Publication of EP4225911A4 publication Critical patent/EP4225911A4/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137—Metal/ion, e.g. metal label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP20956871.6A 2020-10-05 2020-10-05 Plateforme nanoporeuse prête à l'emploi pour la détection d'adn/arn attomole à l'aide d'une sonde complémentaire marquée à l'osmium Pending EP4225911A4 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2020/054271 WO2022075965A1 (fr) 2020-10-05 2020-10-05 Plateforme nanoporeuse prête à l'emploi pour la détection d'adn/arn attomole à l'aide d'une sonde complémentaire marquée à l'osmium

Publications (2)

Publication Number Publication Date
EP4225911A1 EP4225911A1 (fr) 2023-08-16
EP4225911A4 true EP4225911A4 (fr) 2024-07-31

Family

ID=81127042

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20956871.6A Pending EP4225911A4 (fr) 2020-10-05 2020-10-05 Plateforme nanoporeuse prête à l'emploi pour la détection d'adn/arn attomole à l'aide d'une sonde complémentaire marquée à l'osmium

Country Status (10)

Country Link
EP (1) EP4225911A4 (fr)
JP (1) JP2024500005A (fr)
KR (1) KR20230080472A (fr)
CN (1) CN116710572A (fr)
AR (1) AR123690A1 (fr)
AU (1) AU2020471747A1 (fr)
BR (1) BR112023006304A2 (fr)
CA (1) CA3198227A1 (fr)
TW (1) TW202229561A (fr)
WO (1) WO2022075965A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112481363B (zh) * 2020-03-09 2024-05-28 南京大学 突变Aerolysin单体在检测RNA碱基序列及RNA修饰方面的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017173392A1 (fr) * 2016-03-31 2017-10-05 Two Pore Guys, Inc. Différenciation, à travers des nanopores, de polynucléotides cibles d'un arrière-plan d'échantillon par fragmentation et liaison de charge utile

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170137457A1 (en) * 2015-11-18 2017-05-18 Anastassia Kanavarioti Labeled Nucleic Acids: A Surrogate for Nanopore-based Nucleic Acid Sequencing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017173392A1 (fr) * 2016-03-31 2017-10-05 Two Pore Guys, Inc. Différenciation, à travers des nanopores, de polynucléotides cibles d'un arrière-plan d'échantillon par fragmentation et liaison de charge utile

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KANG ALBERT S ET AL: "Ready-to-use nanopore platform for the detection of any DNA/RNA oligo at attomole range using an Osmium tagged complementary probe", SCIENTIFIC REPORTS, vol. 10, no. 1, 1 December 2020 (2020-12-01), US, XP093174477, ISSN: 2045-2322, DOI: 10.1038/s41598-020-76667-1 *
See also references of WO2022075965A1 *
ZAHID OSAMA K ET AL: "Sequence-Specific Recognition of MicroRNAs and Other Short Nucleic Acids with Solid-State Nanopores", NANO LETTERS, vol. 16, no. 3, 9 March 2016 (2016-03-09), US, pages 2033 - 2039, XP093174431, ISSN: 1530-6984, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367926/pdf/nihms802626.pdf> DOI: 10.1021/acs.nanolett.6b00001 *

Also Published As

Publication number Publication date
AR123690A1 (es) 2023-01-04
WO2022075965A1 (fr) 2022-04-14
KR20230080472A (ko) 2023-06-07
EP4225911A1 (fr) 2023-08-16
AU2020471747A1 (en) 2023-05-04
CA3198227A1 (fr) 2022-04-14
JP2024500005A (ja) 2024-01-04
TW202229561A (zh) 2022-08-01
CN116710572A (zh) 2023-09-05
BR112023006304A2 (pt) 2023-05-09
AU2020471747A9 (en) 2024-10-10

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