EP4212628C0 - Nucleic acid sequencing methods - Google Patents
Nucleic acid sequencing methodsInfo
- Publication number
- EP4212628C0 EP4212628C0 EP23155047.6A EP23155047A EP4212628C0 EP 4212628 C0 EP4212628 C0 EP 4212628C0 EP 23155047 A EP23155047 A EP 23155047A EP 4212628 C0 EP4212628 C0 EP 4212628C0
- Authority
- EP
- European Patent Office
- Prior art keywords
- nucleic acid
- acid sequencing
- sequencing methods
- methods
- nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES23155047T ES3014460T3 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP23155047.6A EP4212628B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP25154432.6A EP4553171A3 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP23155047.6A EP4212628B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| PCT/CN2017/095520 WO2019023951A1 (en) | 2017-08-01 | 2017-08-01 | METHOD OF SEQUENCE OF NUCLEIC ACID |
| EP17920285.8A EP3663407B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
Related Parent Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP17920285.8A Division EP3663407B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP17920285.8A Division-Into EP3663407B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP25154432.6A Division EP4553171A3 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| EP4212628A1 EP4212628A1 (en) | 2023-07-19 |
| EP4212628C0 true EP4212628C0 (en) | 2025-01-29 |
| EP4212628B1 EP4212628B1 (en) | 2025-01-29 |
Family
ID=65232180
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP23155059.1A Active EP4209597B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP17920285.8A Active EP3663407B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP23155047.6A Active EP4212628B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP25154434.2A Pending EP4553172A3 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP25154432.6A Pending EP4553171A3 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP23155059.1A Active EP4209597B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP17920285.8A Active EP3663407B1 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP25154434.2A Pending EP4553172A3 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
| EP25154432.6A Pending EP4553171A3 (en) | 2017-08-01 | 2017-08-01 | Nucleic acid sequencing method |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US11692221B2 (en) |
| EP (5) | EP4209597B1 (en) |
| CN (5) | CN118086480A (en) |
| DK (1) | DK3663407T3 (en) |
| ES (3) | ES2941083T3 (en) |
| FI (1) | FI3663407T3 (en) |
| HU (1) | HUE061474T2 (en) |
| PL (1) | PL3663407T3 (en) |
| PT (1) | PT3663407T (en) |
| RS (1) | RS64086B1 (en) |
| WO (1) | WO2019023951A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11959074B2 (en) * | 2020-11-14 | 2024-04-16 | Life Technologies Corporation | System and method for automated repeat sequencing |
| US20230051226A1 (en) | 2021-08-10 | 2023-02-16 | Mgi Tech Co., Ltd. | Flow cell imaging systems and methods, and flow cells and other substrates for use in the same |
| CN115433675B (en) * | 2022-08-03 | 2024-02-23 | 深圳赛陆医疗科技有限公司 | Nucleic acid sequencing system and sequencing control method |
| WO2025137866A1 (en) * | 2023-12-26 | 2025-07-03 | 深圳华大智造科技股份有限公司 | Recovery method for nucleic acid-containing solution used for sequencing on machine |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006678A1 (en) * | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5639603A (en) | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
| JP3419850B2 (en) * | 1993-10-26 | 2003-06-23 | 株式会社日立製作所 | Nucleic acid sorting method |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| DE69928265T3 (en) | 1998-07-30 | 2013-11-28 | Illumina Cambridge Ltd. | MATRICES OF BIOMOLECULES AND THEIR USE IN SEQUENCING |
| AR021833A1 (en) | 1998-09-30 | 2002-08-07 | Applied Research Systems | METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID |
| DK1218545T3 (en) | 1999-08-18 | 2012-02-20 | Illumina Inc | Methods of Preparation of Oligonucleotide Solutions |
| US6274320B1 (en) * | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| WO2001062982A2 (en) | 2000-02-25 | 2001-08-30 | Mosaic Technologies, Inc. | Methods for multi-stage solid phase amplification of nucleic acids |
| WO2002029003A2 (en) | 2000-10-06 | 2002-04-11 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding dna and rna |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| ES2550513T3 (en) | 2002-08-23 | 2015-11-10 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
| EP3438116B1 (en) | 2002-08-23 | 2021-02-17 | Illumina Cambridge Limited | Labelled nucleotides |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| CN101189345A (en) | 2005-02-01 | 2008-05-28 | Ab先进基因分析公司 | Reagents, Methods, and Libraries for Bead-Based Sequencing |
| EP2857523A1 (en) | 2005-02-01 | 2015-04-08 | Applied Biosystems, LLC | Method for identifying a sequence in a polynucleotide |
| US20060246576A1 (en) * | 2005-04-06 | 2006-11-02 | Affymetrix, Inc. | Fluidic system and method for processing biological microarrays in personal instrumentation |
| EP1888743B1 (en) | 2005-05-10 | 2011-08-03 | Illumina Cambridge Limited | Improved polymerases |
| WO2007120208A2 (en) * | 2005-11-14 | 2007-10-25 | President And Fellows Of Harvard College | Nanogrid rolling circle dna sequencing |
| US20070231823A1 (en) | 2006-03-23 | 2007-10-04 | Mckernan Kevin J | Directed enrichment of genomic DNA for high-throughput sequencing |
| EP4105644A3 (en) | 2006-03-31 | 2022-12-28 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| CN101495654A (en) * | 2006-04-19 | 2009-07-29 | 阿普里拉股份有限公司 | Reagents, methods, and libraries for gel-free bead-based sequencing |
| WO2009046149A1 (en) * | 2007-10-01 | 2009-04-09 | Applied Biosystems Inc. | Chase ligation sequencing |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| US10837879B2 (en) | 2011-11-02 | 2020-11-17 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
| KR102230831B1 (en) | 2013-03-15 | 2021-03-22 | 일루미나 케임브리지 리미티드 | Modified nucleosides or nucleotides |
| CN103993100B (en) * | 2014-06-17 | 2017-02-15 | 东南大学 | Method for improving accuracy of gene connecting and sequencing |
-
2017
- 2017-08-01 ES ES17920285T patent/ES2941083T3/en active Active
- 2017-08-01 CN CN202410439618.1A patent/CN118086480A/en active Pending
- 2017-08-01 PT PT179202858T patent/PT3663407T/en unknown
- 2017-08-01 HU HUE17920285A patent/HUE061474T2/en unknown
- 2017-08-01 CN CN201780093472.8A patent/CN111032883B/en active Active
- 2017-08-01 EP EP23155059.1A patent/EP4209597B1/en active Active
- 2017-08-01 CN CN202410439435.XA patent/CN118086479A/en active Pending
- 2017-08-01 ES ES23155059T patent/ES3018947T3/en active Active
- 2017-08-01 US US16/635,131 patent/US11692221B2/en active Active
- 2017-08-01 CN CN202410439616.2A patent/CN118109572A/en active Pending
- 2017-08-01 EP EP17920285.8A patent/EP3663407B1/en active Active
- 2017-08-01 ES ES23155047T patent/ES3014460T3/en active Active
- 2017-08-01 FI FIEP17920285.8T patent/FI3663407T3/en active
- 2017-08-01 EP EP23155047.6A patent/EP4212628B1/en active Active
- 2017-08-01 PL PL17920285.8T patent/PL3663407T3/en unknown
- 2017-08-01 RS RS20230242A patent/RS64086B1/en unknown
- 2017-08-01 WO PCT/CN2017/095520 patent/WO2019023951A1/en not_active Ceased
- 2017-08-01 DK DK17920285.8T patent/DK3663407T3/en active
- 2017-08-01 EP EP25154434.2A patent/EP4553172A3/en active Pending
- 2017-08-01 CN CN202410439381.7A patent/CN118086478A/en active Pending
- 2017-08-01 EP EP25154432.6A patent/EP4553171A3/en active Pending
-
2023
- 2023-05-05 US US18/144,121 patent/US20230272467A1/en active Pending
- 2023-05-05 US US18/144,124 patent/US20230323445A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20230323445A1 (en) | 2023-10-12 |
| PL3663407T3 (en) | 2023-05-08 |
| EP4553171A2 (en) | 2025-05-14 |
| US20200318177A1 (en) | 2020-10-08 |
| DK3663407T3 (en) | 2023-04-03 |
| ES3014460T3 (en) | 2025-04-22 |
| CN111032883B (en) | 2024-05-03 |
| CN118109572A (en) | 2024-05-31 |
| EP3663407A4 (en) | 2021-03-03 |
| EP4553172A2 (en) | 2025-05-14 |
| EP4209597B1 (en) | 2025-02-26 |
| CN118086480A (en) | 2024-05-28 |
| CN111032883A (en) | 2020-04-17 |
| EP4209597C0 (en) | 2025-02-26 |
| PT3663407T (en) | 2023-03-27 |
| EP4212628A1 (en) | 2023-07-19 |
| EP4209597A1 (en) | 2023-07-12 |
| EP4553171A3 (en) | 2025-10-22 |
| FI3663407T3 (en) | 2023-04-18 |
| CN118086479A (en) | 2024-05-28 |
| CN118086478A (en) | 2024-05-28 |
| WO2019023951A1 (en) | 2019-02-07 |
| HUE061474T2 (en) | 2023-07-28 |
| EP4553172A3 (en) | 2025-06-25 |
| RS64086B1 (en) | 2023-04-28 |
| EP3663407B1 (en) | 2023-03-15 |
| EP3663407A1 (en) | 2020-06-10 |
| ES2941083T3 (en) | 2023-05-16 |
| US20230272467A1 (en) | 2023-08-31 |
| ES3018947T3 (en) | 2025-05-19 |
| EP4212628B1 (en) | 2025-01-29 |
| US11692221B2 (en) | 2023-07-04 |
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