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EP4263778A2 - Élimination de conaimination et de recontamination virales par les coronaviridae (y compris sars-cov-2) avec des solutions hygiéniques à base de probiotiques - Google Patents

Élimination de conaimination et de recontamination virales par les coronaviridae (y compris sars-cov-2) avec des solutions hygiéniques à base de probiotiques

Info

Publication number
EP4263778A2
EP4263778A2 EP21886990.7A EP21886990A EP4263778A2 EP 4263778 A2 EP4263778 A2 EP 4263778A2 EP 21886990 A EP21886990 A EP 21886990A EP 4263778 A2 EP4263778 A2 EP 4263778A2
Authority
EP
European Patent Office
Prior art keywords
probiotic
coronaviridae
cov
test
bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21886990.7A
Other languages
German (de)
English (en)
Inventor
Johannes VAN OVERVELD
Tim KEMLAND
Gjorgji JOVANOVSKI
Natalija ATANASOVA-PANCEVSKA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Investstone Doo Skopje
Original Assignee
Investstone Doo Skopje
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Investstone Doo Skopje filed Critical Investstone Doo Skopje
Publication of EP4263778A2 publication Critical patent/EP4263778A2/fr
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/381—Microorganisms
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions

Definitions

  • the invention relates to probiotic hygiene solutions and methods for the use of probiotic non- pathogenic bacteria as an innovative solution for efficient cleaning, sanitation, disinfection and protection against contamination, elimination and re-contamination and of Coronaviridae (family of Corona viruses, including SARS-Cov-2, causing a global pandemic of Covid 19) with immediate, residual and prolonged efficacy applicable to hard surfaces, soft surfaces, human and animal skin, plants and the general environment as a natural-based product with numerous health and environmental benefits.
  • Coronaviridae family of Corona viruses, including SARS-Cov-2, causing a global pandemic of Covid 19
  • the invention is in the field of human needs, chemistry and procedures, which according to the International Patent Classification belongs to the:
  • Sub area preparations for medical and cosmetic use i.e. medicines containing probiotics and in the sub area of microbiology, i.e. measurement and testing of procedures involving microorganisms (bacteria).
  • the invention can be classified into the following classes: A61K 35/741 , A61L 2/18, C12Q 1/00, C12Q 1/02, C12Q 1/689, B08B 3/04, B05D 1/00.
  • probiotic hygienic solution is based on biological active substance and has the following new applications:
  • the invention is tested via probiotic hand spray, which is able to reduce and, in less than one minute, completely eliminate the growth of SARS-Cov-2 virus.
  • the product has also general antimicrobial properties and has been tested against other most common pathogenic microbial species namely: Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9037, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404.
  • probiotic solution is able to reduce and, completely eliminate the growth of specific pathogenic microbial species namely Coronaviridae family of viruses, including the novel SARS-Cov-2.
  • the in vitro tests allowed the reduction in microbial count of the pathogens to be verified under controlled conditions, thus verifying the efficacy of tested Probiotic Hand spray to combat microorganisms where there is no risk of recontamination.
  • the results achieved confirms that the continuous and constant action of this probiotic-based solution is able to reduce and eliminate the proliferation of tested microorganisms.
  • this invention is effective and innovative product for environmental management.
  • Probiotics are ecological, easy to use and biodegradable. They render the skin, surfaces and the overall environment hygienically stable and are able to survive on and colonize non biological surfaces, combatting the proliferation of Coronaviridae viruses and other pathogens. In this study they were also found to perform well on different surfaces. Probiotics are therefore effective innovative products for sanitizing different environment and constitute a valid “green” alternative to the chemical disinfectants used up to now.
  • EP2556823 A review of the European patent application EP2556823 has established that there are cosmetic products that contain probiotic microorganisms as cleaning agents, but also contain chemical ingredients such as alkyl sulfate and alkyl polyglycol cholesterol sulphate salts. They are used to improve the skin barrier. Compared to the invention, it does not refer to cleaning or disinfecting the surface from viral contamination, i.e. SARS-Cov-2 (corona virus).
  • Russian patent number RU2699540 C2 relates to compositions containing probiotic bacteria and procedures for their use in the treatment of immune skin diseases.
  • Any surface or environment can be contaminated with a variety of microorganisms derived from various sources, some of them pathogen (harmful) to humans.
  • a pathogen is the term used to describe an infectious microorganism or agent, such as a virus, bacterium, protozoan, prion, viroid or fungus; or this is an organism that causes disease.
  • viruses are submicroscopic infectious agent that replicates only inside the living cells of an organism. When not inside an infected cell or in the process of infecting a cell, viruses exist in the form of independent particles, or virions, consisting of genetic material (DNA or RNA) and a protein coat.
  • Probiotics are live microorganisms promoted with claims that they provide health and environmental benefits or in the case of this patent application as proven by the microbiological tests provided in this patent application, non-pathogen good bacteria safe for handling and use that promote biological dirt degradation and anti-microbial and antiviral disinfection and after-protection. Probiotics are even considered generally safe to consume.
  • the novel SARS-Cov-2 (Corona) virus that causes Covid-19 infections belongs to Coronaviridae family of viruses. Its particles (or “virions”) are spheres with diameters of approximately 0.125 microns (125 nm). The smallest particles are 0.06 microns, and the largest are 0.14 microns. Coronaviridae Viruses can infect all types of life forms: animals, plants and humans and can survive on hard and soft surfaces for up to 48 hours mainly due to the biofilm created by pathogen (bad) bacteria which virus is using as a host.
  • the invention is new break-through solution composed of probiotic non-pathogen bacteria as an innovative solution for efficient cleaning, sanitation, disinfection and protection against contamination, elimination and re-contamination with Coronaviridae (Corona-family of viruses, including SARS-Cov-2 virus that causes the pandemic of Covid 19) with immediate in less than 60 seconds and time-extended efficacy applicable on hard surfaces, soft surfaces, human and animal skin, plants and the general environment as a natural-based product with numerous health and ecological benefits. They offer hours or days of effective residual after-protection against Coronaviridae viruses after their application on abovementioned skin or surfaces.
  • the solution is aqueous active substance of Probiotic non-pathogen bacteria belonging to Class 1 EU / Generally Regarded As Safe and mixed with water - resulting in sufficient end-concentrations of viable probiotic bacteria. Therefore it provides non-toxic activity to skin or surfaces - due to absence of chemical components.
  • the application method of such innovative cleaning, sanitation, disinfection and protection solutions is to be made by means of manual mechanical application, mopping, rinsing, spraying or machine application by spraying trough nozzles in hand-disinfection machines, disinfection tunnels, spray cabins or any external human application by air- borne solution particles.
  • probiotic-based cleaning products employ the capability of living microorganisms to produce extracellular enzymes, such as cellulases, proteases and ureases. These enzymes can degrade high molecular weight compounds often associated with dirt and effectively mitigate unfavourable odours by further metabolism of intermediates from these processes.
  • surfactins produced by various Bacillus species have detergent-like properties and have been shown to exhibit antimicrobial properties by interacting with bio-membranes (Bartal et al., 2018).
  • Other microorganisms such as the lactic acid bacteria can inhibit growth by lowering the pH in its environment (Dural-Erem et al., 2019).
  • a synergy of various mechanisms could come into play when it comes to the cleaning effect of these probiotic-based products (Spbk et al., 2018).
  • Disk diffusion test A clear zone or ring is formed around the disk after incubation if the agent inhibits microbial growth.
  • Figure 2 Protocol for testing protection time: in order to assess how effectively probiotics were able to compete against above mentioned microbial strains in the absence of external factors linked to recontamination.
  • the two surfaces floor and desk, with area of 30*30 cm
  • Samples of each microorganism were taken daily over a five-days period and inoculated on agar medium. Then bacterial strains were incubated at 37°C for 18-24 hours, and fungal strains were incubated at room temperature, 3-5 days.
  • Figure 3 The product has also general antimicrobial properties and has been tested against other most common pathogenic microbial species namely: antimicrobial activity of probiotic based solution (original and diluted 1 :10 Handspray against Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9037, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404.
  • probiotic based solution original and diluted 1 :10 Handspray against Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9037, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404.
  • the first test is a PCR sampling test on a surface populated with SARS-Cov-2. According to the analytical rapport i.e. efficiency test of probiotic hand spray vis-a-vis Sars-Cov-2 performed by TLR International laboratories in Rotterdam is confirmed that after use of probiotic hand spray there is no detection of Coronaviridae / SARS-Cov-2.
  • the second test EN 1500 disinfection standard test for hand-rub, EU standard is aimed to evaluate and compare the efficacy of probiotic-based hand soap and hand spray in reducing the microbial load present on artificially contaminated hands; and to compare the antimicrobial efficacy of the tested products with those of reference indicated in the methodology of EN1499 and EN1500, which used the non-antimicrobial soft soap and propan-2-ol 60% (v/v).
  • the EN 1499 and EN 1500 are a European Standard test methods that evaluates the efficacy of a hygienic handwash and handrub by measuring the number of viable bacteria remaining on the fingertips after contamination and handwash (handrub) exposure.
  • the EN 1499 standard is used to test antiseptic soaps and requires 12-15 subjects.
  • the EN 1500 is used to test antiseptic hand rubs and requires 18-22 subjects. Experimental contamination is with a non-pathogenic strain of Escherichia coli. Subjects are allotted randomly to two groups of approximately the same size, where one group applies the test formulation, and the other a reference antiseptic solution using a standardised procedure.
  • the mean reduction caused by the test product in the numbers of viable bacteria shall be significantly higher than that obtained with the non-antimicrobial soft soap.
  • the mean reduction in the numbers of viable bacteria shall not be significantly inferior to that with the reference alcohol-based hand rub (propan-2-ol 60% (v/v)).
  • the pre- and post-values recovered from the fingertips are evaluated against one another, resulting in a ratio called the reduction factor.
  • the reduction factor provides a quantitative measure of the antimicrobial efficacy. This is determined by comparing average counts of viable microorganism colony forming units of the pre-values to the average counts after exposure to the hygienic handrub.
  • Probiotic-based products hand soap and hand spray, were used in experiments for reducing the microbial load present on artificially contaminated hands, according to protocol EN1499 and EN1500.
  • biotechnology cleaning products contain non-pathogenic probiotic bacteria. These nonpathogenic bacteria are considered as a GRAS (generally recognized as safe) organism as they do not deemed to cause human diseases, with no impact on plants or animals. They also produce antimicrobial components active against many dangerous pathogenic bacteria. Due to their safety and activity on pathogens, biotechnology products have been used probiotic sanitation, demonstrating a significant reduction in microbial contamination with a stable effect over time.
  • GRAS generally recognized as safe
  • the EN1499 European Standard specifies a test method simulating practical conditions for establishing whether a product for hygienic handwash reduces the release of transient microbial flora on hands when used to wash the artificially contaminated hands of volunteers. In our study, we used 20 people for all tests. When tested in accordance with this test, the mean reduction of the release of the test organism Escherichia coli achieved by the hygienic handwash with the product under test shall be larger than that achieved by a specified reference hygienic handwash (non-antimicrobial soft soap).
  • the log reduction rate and % of reduction of the test microorganism after application of the probiotic-based hand soap and non-antimicrobial soft soap is shown in Table 1.
  • probiotic-based hand soap when applied in the manner described meet the requirement stipulated in EN 1499 i.e. the mean reduction of the release of test organisms achieved by the tested product shall be larger than that achieved by the reference product (non-antimicrobial soft soap) and therefore passes the European Standard EN 1499 for a hygienic handwash.
  • the E 1500 European Standard specifies a test method simulating practical conditions for establishing whether a product for hygienic handrub reduces the release of transient microbial flora on hands when rubbed onto the artificially contaminated hands of volunteers.
  • the mean reduction of the release of the test organism Escherichia coli achieved by the hygienic handrub with the product under test shall be at least not inferior to that achieved by a specified reference hygienic handrub (60 % volume concentration of propan-2-ol).
  • the log reduction rate and % of reduction of the test microorganism after application of the probiotic-based hand spray and propan-2-ol (60%) is shown in Table 2.
  • probiotic-based hand spray when applied and rubbed into the hands get reduction of about 99.9 % (log reduction of 3.697651187), higher than the reduction % of the reference product propan-2-ol 60% (w/v) (log reduction of 3.518963).
  • probiotic-based hand spray when applied in the manner described in the Standard meets the requirement stipulated in EN 1500- the mean reduction of the release of test organisms achieved by the test product is not significantly smaller than that achieved by the reference product (propan-2-ol 60% w/v) and therefore passes the European Standard EN 1500 for a hygienic hand rub.
  • biotechnology cleaning products containing probiotic bacteria was found effective in reducing the number of viable bacteria remaining on the fingertips after artificial contamination. They are eco-friendly; thus, they can reduce the exposure to chemical disinfectants. It is necessary to establish a new biosafety protocol which includes adequate disinfection by appropriate cleaning products. The information obtained from this study can be used to develop proper interventions to improve disinfection practices in all aspects of our lives. The only demand set by this method is that the frequency of disinfection is kept constant, so the probiotic bacteria (the good bacteria).

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Detergent Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

L'invention concerne des solutions d'hygiène à base de probiotiques, composées de bactéries probiotiques non pathogènes en tant que solution innovante, destinées à un nettoyage, un assainissement, une désinfection et une protection efficaces, pour faire face, par élimination, à une contamination et une recontamination par les Coronaviridae (famille de Coronavirus, y compris le SARS-Cov-2 provoquant une pandémie mondiale de Covid 19), avec une protection immédiate et une après-protection résiduelle étendue (en heures ou en jours) efficaces, et pouvant s'appliquer sur des surfaces dures, des surfaces souples, sur la peau humaine et animale, sur des plantes et l'environnement général, en tant que produit d'origine naturelle présentant de nombreux avantages sanitaires et environnementaux.
EP21886990.7A 2020-11-02 2021-11-01 Élimination de conaimination et de recontamination virales par les coronaviridae (y compris sars-cov-2) avec des solutions hygiéniques à base de probiotiques Pending EP4263778A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MK85720 2020-11-02
PCT/MK2021/000004 WO2022092999A2 (fr) 2020-11-02 2021-01-01 Élimination de conaimination et de recontamination virales par les coronaviridae (y compris sars-cov-2) avec des solutions hygiéniques à base de probiotiques

Publications (1)

Publication Number Publication Date
EP4263778A2 true EP4263778A2 (fr) 2023-10-25

Family

ID=81384459

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21886990.7A Pending EP4263778A2 (fr) 2020-11-02 2021-11-01 Élimination de conaimination et de recontamination virales par les coronaviridae (y compris sars-cov-2) avec des solutions hygiéniques à base de probiotiques

Country Status (2)

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EP (1) EP4263778A2 (fr)
WO (1) WO2022092999A2 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011080715A1 (de) * 2011-08-10 2013-02-14 Henkel Ag & Co. Kgaa Hautbarrierestärkendes Reinigungsmittel
CN108347947A (zh) * 2015-10-30 2018-07-31 以Z生物科学公司名义营业的Z益生菌公司 益生菌组合物及其用途
RU2699540C2 (ru) * 2017-08-17 2019-09-06 Павел Павлович Несмиянов Композиция, содержащая пробиотические бактерии или их компоненты, и способ ее применения в лечении иммунных заболеваний кожи

Also Published As

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WO2022092999A8 (fr) 2022-07-14
WO2022092999A2 (fr) 2022-05-05
WO2022092999A3 (fr) 2022-11-03

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