EP4114941A4 - Improved methods and compositions for modulating a genome - Google Patents
Improved methods and compositions for modulating a genome Download PDFInfo
- Publication number
- EP4114941A4 EP4114941A4 EP21764647.0A EP21764647A EP4114941A4 EP 4114941 A4 EP4114941 A4 EP 4114941A4 EP 21764647 A EP21764647 A EP 21764647A EP 4114941 A4 EP4114941 A4 EP 4114941A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- genome
- modulating
- compositions
- improved methods
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Enzymes And Modification Thereof (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cosmetics (AREA)
- Toys (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062985264P | 2020-03-04 | 2020-03-04 | |
| US202063035674P | 2020-06-05 | 2020-06-05 | |
| PCT/US2021/020943 WO2021178717A2 (en) | 2020-03-04 | 2021-03-04 | Improved methods and compositions for modulating a genome |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP4114941A2 EP4114941A2 (en) | 2023-01-11 |
| EP4114941A4 true EP4114941A4 (en) | 2024-10-16 |
Family
ID=77613826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP21764647.0A Pending EP4114941A4 (en) | 2020-03-04 | 2021-03-04 | Improved methods and compositions for modulating a genome |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US20240018551A1 (en) |
| EP (1) | EP4114941A4 (en) |
| JP (2) | JP2023517294A (en) |
| KR (1) | KR20230053735A (en) |
| CN (1) | CN116209770A (en) |
| AU (1) | AU2021230545A1 (en) |
| BR (1) | BR112022017736A2 (en) |
| CA (1) | CA3174483A1 (en) |
| IL (1) | IL296095A (en) |
| WO (1) | WO2021178717A2 (en) |
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| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| WO2020047124A1 (en) | 2018-08-28 | 2020-03-05 | Flagship Pioneering, Inc. | Methods and compositions for modulating a genome |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| AU2020240109A1 (en) | 2019-03-19 | 2021-09-30 | President And Fellows Of Harvard College | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CA3149897A1 (en) | 2019-09-03 | 2021-03-11 | Daniel Getts | Methods and compositions for genomic integration |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| IL296024A (en) | 2020-03-04 | 2022-10-01 | Flagship Pioneering Innovations Vi Llc | Methods and compositions for genome modulation |
| IL296095A (en) | 2020-03-04 | 2022-11-01 | Flagship Pioneering Innovations Vi Llc | Improved methods and compositions for modulating a genome |
| BR112022022603A2 (en) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | METHODS AND COMPOSITIONS FOR SIMULTANEOUS EDITING OF BOTH DUAL-STRANDED NUCLEOTIDE TARGET SEQUENCE STRAINS |
| IL308445A (en) | 2021-05-11 | 2024-01-01 | Myeloid Therapeutics Inc | Methods and compositions for genomic integration |
| MX2024002927A (en) | 2021-09-08 | 2024-05-29 | Flagship Pioneering Innovations Vi Llc | Methods and compositions for modulating a genome. |
| CN113755627B (en) * | 2021-09-24 | 2023-06-16 | 广西大学 | Primer based on cis-regulatory element, design method thereof and application thereof in molecular marking |
| CN114277119B (en) * | 2021-12-09 | 2023-05-16 | 浙江大学医学院附属邵逸夫医院 | Application of circular RNA circ-Arsb in preparation of osteoporosis prevention and treatment product |
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| IL316725A (en) * | 2022-05-02 | 2024-12-01 | Univ California | Multicomponent systems for site-specific genome modifications |
| CN116334141A (en) * | 2022-08-04 | 2023-06-27 | 北京中因科技有限公司 | RHO-R135W-adrP gene editing medicine based on gene editing |
| CN116064797B (en) * | 2022-08-29 | 2023-10-20 | 广州达健生物科技有限公司 | Endometrial cancer gene methylation level detection reagent and application thereof |
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| WO2024131786A1 (en) * | 2022-12-19 | 2024-06-27 | 北京干细胞与再生医学研究院 | System for inserting large fragment dna into genome |
| WO2024137990A2 (en) * | 2022-12-21 | 2024-06-27 | Trustees Of Boston University | Compositions and methods for controlled mrna translation and stability |
| CN116179513B (en) * | 2023-03-10 | 2023-12-22 | 之江实验室 | A Cpf1 protein and its application in gene editing |
| WO2024196963A2 (en) * | 2023-03-21 | 2024-09-26 | The Regents Of The University Of California | Compositions and methods for genome editing |
| US20240352485A1 (en) | 2023-04-19 | 2024-10-24 | Flagship Pioneering Innovations Vii, Llc | Compositions and methods for the production of libraries |
| CN116523902B (en) * | 2023-06-21 | 2023-09-26 | 湖南盛鼎科技发展有限责任公司 | Electronic powder coating uniformity detection method and device based on improved YOLOV5 |
| WO2025006419A1 (en) | 2023-06-26 | 2025-01-02 | Flagship Pioneering Innovations Vii, Llc | Engineered plasmodia and related methods |
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| EP4430188A1 (en) | 2021-11-09 | 2024-09-18 | Prime Medicine, Inc. | Genome editing compositions and methods for treatment of amyotrophic lateral sclerosis |
| EP4430189A1 (en) | 2021-11-11 | 2024-09-18 | Prime Medicine, Inc. | Genome editing compositions and methods for treatment of fragile x syndrome |
| WO2023096977A2 (en) | 2021-11-24 | 2023-06-01 | Prime Medicine, Inc. | Modified prime editing guide rnas |
| EP4437106A2 (en) | 2021-11-24 | 2024-10-02 | Prime Medicine, Inc. | Methods and compositions for inhibiting mismatch repair |
| WO2023192655A2 (en) | 2022-04-01 | 2023-10-05 | Prime Medicine, Inc. | Methods and compositions for editing nucleotide sequences |
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2021
- 2021-03-04 IL IL296095A patent/IL296095A/en unknown
- 2021-03-04 JP JP2022552817A patent/JP2023517294A/en active Pending
- 2021-03-04 EP EP21764647.0A patent/EP4114941A4/en active Pending
- 2021-03-04 WO PCT/US2021/020943 patent/WO2021178717A2/en not_active Ceased
- 2021-03-04 KR KR1020227034440A patent/KR20230053735A/en active Pending
- 2021-03-04 CA CA3174483A patent/CA3174483A1/en active Pending
- 2021-03-04 AU AU2021230545A patent/AU2021230545A1/en active Pending
- 2021-03-04 CN CN202180035530.8A patent/CN116209770A/en active Pending
- 2021-03-04 BR BR112022017736A patent/BR112022017736A2/en unknown
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2022
- 2022-09-02 US US17/929,455 patent/US20240018551A1/en active Pending
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2023
- 2023-09-14 US US18/467,392 patent/US12065669B2/en active Active
- 2023-09-14 US US18/467,428 patent/US12037602B2/en active Active
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2025
- 2025-08-27 JP JP2025141096A patent/JP2025172847A/en active Pending
Non-Patent Citations (3)
| Title |
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| S. M. CHRISTENSEN ET AL: "RNA from the 5' end of the R2 retrotransposon controls R2 protein binding to and cleavage of its DNA target site", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 103, no. 47, 21 November 2006 (2006-11-21), pages 17602 - 17607, XP055659527, ISSN: 0027-8424, DOI: 10.1073/pnas.0605476103 * |
| SEA URCHIN GENOME SEQUENCING CONSORTIUM: "The genome of the sea urchin Strongylocentrotus purpuratus", 20061110, vol. 314, no. 5801, 10 November 2006 (2006-11-10), pages 941 - 952, XP008120409, DOI: 10.1126/SCIENCE.1133609 * |
| TAY WEE TEK ET AL: "Generation of microsatellite repeat families by RTE retrotransposons in lepidopteran genomes", BMC EVOLUTIONARY BIOLOGY, BIOMED CENTRAL LTD., LONDON, GB, vol. 10, no. 1, 17 May 2010 (2010-05-17), pages 144, XP021072205, ISSN: 1471-2148, DOI: 10.1186/1471-2148-10-144 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA3174483A1 (en) | 2021-09-10 |
| US20240084333A1 (en) | 2024-03-14 |
| AU2021230545A1 (en) | 2022-09-29 |
| JP2025172847A (en) | 2025-11-26 |
| CN116209770A (en) | 2023-06-02 |
| US20240018551A1 (en) | 2024-01-18 |
| BR112022017736A2 (en) | 2022-11-29 |
| US20240076698A1 (en) | 2024-03-07 |
| JP2023517294A (en) | 2023-04-25 |
| WO2021178717A2 (en) | 2021-09-10 |
| US12065669B2 (en) | 2024-08-20 |
| EP4114941A2 (en) | 2023-01-11 |
| KR20230053735A (en) | 2023-04-21 |
| US12037602B2 (en) | 2024-07-16 |
| WO2021178717A9 (en) | 2022-03-17 |
| IL296095A (en) | 2022-11-01 |
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