EP4146820A4 - METHODS FOR DETECTING PATHOGENIC INFECTIONS USING PATIENT SAMPLES CONTAINING RED BLOOD CELLS - Google Patents

METHODS FOR DETECTING PATHOGENIC INFECTIONS USING PATIENT SAMPLES CONTAINING RED BLOOD CELLS Download PDF

Info

Publication number: EP4146820A4
Authority: EP; European Patent Office
Prior art keywords: methods; blood cells; red blood; samples containing; patient samples
Prior art date: 2020-05-08
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

EP21800751.6A

Other languages

German (de)

French (fr)

Other versions

EP4146820A1 (en

Inventor

Nilam MANGALMURTI

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

University of Pennsylvania Penn

Original Assignee

University of Pennsylvania Penn

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2020-05-08

Filing date

2021-05-07

Publication date

2024-09-11

2021-05-07 Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn

2023-03-15 Publication of EP4146820A1 publication Critical patent/EP4146820A1/en

2024-09-11 Publication of EP4146820A4 publication Critical patent/EP4146820A4/en

Status Pending legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4716—Complement proteins, e.g. anaphylatoxin, C3a, C5a
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Hematology (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Urology & Nephrology (AREA)
Virology (AREA)
Tropical Medicine & Parasitology (AREA)
Cell Biology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Rehabilitation Therapy (AREA)
Rheumatology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

EP21800751.6A 2020-05-08 2021-05-07 METHODS FOR DETECTING PATHOGENIC INFECTIONS USING PATIENT SAMPLES CONTAINING RED BLOOD CELLS Pending EP4146820A4 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US202063022181P	2020-05-08	2020-05-08
PCT/US2021/031323 WO2021226475A1 (en)	2020-05-08	2021-05-07	Methods for detection of pathogenic infections using red blood cell-containing patient samples

Publications (2)

Publication Number	Publication Date
EP4146820A1 EP4146820A1 (en)	2023-03-15
EP4146820A4 true EP4146820A4 (en)	2024-09-11

Family

ID=78468453

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP21800751.6A Pending EP4146820A4 (en)	2020-05-08	2021-05-07	METHODS FOR DETECTING PATHOGENIC INFECTIONS USING PATIENT SAMPLES CONTAINING RED BLOOD CELLS

Country Status (5)

Country	Link
US (1)	US20230313325A1 (en)
EP (1)	EP4146820A4 (en)
AU (1)	AU2021267932A1 (en)
CA (1)	CA3178218A1 (en)
WO (1)	WO2021226475A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN114836579B (en) *	2022-06-02	2024-05-07	昆明理工大学	Primer combinations for multiplex fluorescence quantitative PCR detection of central nervous system infectious pathogens
CN114959062B (en) *	2022-06-10	2025-06-03	中山大学	A kit for diagnosing schistosomiasis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP3200134B2 (en) *	1992-02-24	2001-08-20	湧永製薬株式会社	Malaria parasite detection
EP2612708B1 (en) *	2007-07-23	2018-12-19	CLONDIAG GmbH	Assays

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CN101541978B (en) *	2006-11-30	2017-12-22	Id-菲什技术公司	Nucleic acid probes and methods for detecting Plasmodium parasites
US10072309B1 (en) *	2015-05-08	2018-09-11	Dougbeh-Chris Nyan	Methods for real-time multiplex isothermal detection and identification of bacterial, viral, and protozoan nucleic acids

2021
- 2021-05-07 US US17/997,742 patent/US20230313325A1/en active Pending
- 2021-05-07 AU AU2021267932A patent/AU2021267932A1/en active Pending
- 2021-05-07 CA CA3178218A patent/CA3178218A1/en active Pending
- 2021-05-07 WO PCT/US2021/031323 patent/WO2021226475A1/en not_active Ceased
- 2021-05-07 EP EP21800751.6A patent/EP4146820A4/en active Pending

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP3200134B2 (en) *	1992-02-24	2001-08-20	湧永製薬株式会社	Malaria parasite detection
EP2612708B1 (en) *	2007-07-23	2018-12-19	CLONDIAG GmbH	Assays

Non-Patent Citations (7)

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J. LOO: "Sample-to-answer on molecular diagnosis of bacterial infection using integrated lab�-on�-a-�disc", BIOSENSORS AND BIOELECTRONICS, vol. 93, 1 July 2017 (2017-07-01), Amsterdam , NL, pages 212 - 219, XP093158581, ISSN: 0956-5663, DOI: 10.1016/j.bios.2016.09.001 *
JEANNE A. JORDAN: "Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis", THE JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 7, no. 5, 1 November 2005 (2005-11-01), pages 575 - 581, XP093158582, ISSN: 1525-1578, DOI: 10.1016/S1525-1578(10)60590-9 *
KOSEI MORIYAMA: "Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood", MICROBIOLOGY AND IMMUNOLOGY, vol. 52, no. 7, 1 July 2008 (2008-07-01), JP, pages 375 - 382, XP093158576, ISSN: 0385-5600, DOI: 10.1111/j.1348-0421.2008.00048.x *
NIELS WUYTS: "A SIMPLIFIED AND HIGHLY SENSITIVE DETECTION OF TRYPANOSOMA EVANS/ BY DNA AMPLIFICATION", vol. 25, no. 2, 1 June 1994 (1994-06-01), pages 266 - 271, XP093158554, Retrieved from the Internet <URL:https://www.semanticscholar.org/paper/A-simplified-and-highly-sensitive-detection-of-by-Wuyts-Chokesajjawatee/f373b40501ae1db0d7b98b179b39e9ba5a9f226bhttps://www.semanticscholar.org/paper/A-simplified-and-highly-sensitive-detection-of-by-Wuyts-Chokesajjawatee/f373b40501ae1db0d7b98b179b39e9ba5a9f226b> *
RAVENHILL BENJAMIN J. ET AL: "Quantitative comparative analysis of human erythrocyte surface proteins between individuals from two genetically distinct populations", COMMUNICATIONS BIOLOGY, vol. 2, no. 1, 1 September 2019 (2019-09-01), pages 350 - 350, XP093295483, ISSN: 2399-3642, DOI: 10.1038/s42003-019-0596-y *
See also references of WO2021226475A1 *
WANG DEGUO: "One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay", BIORXIV, 21 April 2020 (2020-04-21), XP055892452, Retrieved from the Internet <URL:https://www.biorxiv.org/content/10.1101/2020.04.21.052530v1> [retrieved on 20220216], DOI: 10.1101/2020.04.21.052530 *

Also Published As

Publication number	Publication date
EP4146820A1 (en)	2023-03-15
WO2021226475A1 (en)	2021-11-11
US20230313325A1 (en)	2023-10-05
CA3178218A1 (en)	2021-11-11
AU2021267932A1 (en)	2023-01-19

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Kouidhi et al.	2011	Antibiotic resistance and adhesion properties of oral Enterococci associated to dental caries
EP4146820A4 (en)	2024-09-11	METHODS FOR DETECTING PATHOGENIC INFECTIONS USING PATIENT SAMPLES CONTAINING RED BLOOD CELLS
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Belcheva et al.	2012	Transmission of Porphyromonas gingivalis from caregivers to children
Zamani et al.	2008	Frequency of blood transfusion and its products in Emam Hospital of Sari
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Choi et al.	2015	Single-cell morphological analysis for rapid antimicrobial susceptibility test

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2024-06-19	RIC1	Information provided on ipc code assigned before grant	Ipc: G01N 33/53 20060101ALI20240516BHEP Ipc: C12Q 1/689 20180101ALI20240516BHEP Ipc: C12Q 1/6893 20180101AFI20240516BHEP
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