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EP3947731A4 - Composition de liaison multivalente pour l'analyse d'acide nucléique - Google Patents

Composition de liaison multivalente pour l'analyse d'acide nucléique Download PDF

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Publication number
EP3947731A4
EP3947731A4 EP20815612.5A EP20815612A EP3947731A4 EP 3947731 A4 EP3947731 A4 EP 3947731A4 EP 20815612 A EP20815612 A EP 20815612A EP 3947731 A4 EP3947731 A4 EP 3947731A4
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
acid analysis
binding composition
multivalent binding
multivalent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20815612.5A
Other languages
German (de)
English (en)
Other versions
EP3947731A1 (fr
Inventor
Sinan ARSLAN
Molly He
Matthew KELLINGER
Jake Levieux
Michael Previte
Junhua Zhao
Su Zhang
Tyler LOPEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Element Biosciences Inc
Original Assignee
Element Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/579,794 external-priority patent/US10768173B1/en
Application filed by Element Biosciences Inc filed Critical Element Biosciences Inc
Publication of EP3947731A1 publication Critical patent/EP3947731A1/fr
Publication of EP3947731A4 publication Critical patent/EP3947731A4/fr
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/157A reaction step characterised by the number of molecules incorporated or released
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP20815612.5A 2019-05-24 2020-05-22 Composition de liaison multivalente pour l'analyse d'acide nucléique Pending EP3947731A4 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962852876P 2019-05-24 2019-05-24
US201962897172P 2019-09-06 2019-09-06
US16/579,794 US10768173B1 (en) 2019-09-06 2019-09-23 Multivalent binding composition for nucleic acid analysis
PCT/US2020/034409 WO2020243017A1 (fr) 2019-05-24 2020-05-22 Composition de liaison multivalente pour l'analyse d'acide nucléique

Publications (2)

Publication Number Publication Date
EP3947731A1 EP3947731A1 (fr) 2022-02-09
EP3947731A4 true EP3947731A4 (fr) 2022-06-29

Family

ID=73554179

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20815612.5A Pending EP3947731A4 (fr) 2019-05-24 2020-05-22 Composition de liaison multivalente pour l'analyse d'acide nucléique

Country Status (11)

Country Link
EP (1) EP3947731A4 (fr)
JP (2) JP7542000B2 (fr)
KR (3) KR20250096873A (fr)
CN (1) CN113939601A (fr)
AU (3) AU2020285657B2 (fr)
CA (1) CA3137120A1 (fr)
DE (1) DE112020002516B4 (fr)
GB (1) GB2597398B (fr)
IL (3) IL316901A (fr)
SG (1) SG11202112049VA (fr)
WO (1) WO2020243017A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2589496B (en) 2018-06-12 2023-01-25 Element Biosciences Inc Improved reverse transcriptase for nucleic acid sequencing
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
WO2020102766A2 (fr) 2018-11-15 2020-05-22 Element Biosciences, Inc. Procédés de génération de molécules d'acides nucléiques circulaires
DE202019005610U1 (de) 2018-12-07 2021-06-10 Element Biosciences, Inc. Durchflusszellenvorrichtung und ihre Verwendung
US12313627B2 (en) 2019-05-01 2025-05-27 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US11060138B1 (en) 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
US11198121B1 (en) 2020-06-10 2021-12-14 Element Biosciences, Inc. Flow cell systems and devices
GB2599729A (en) * 2020-10-12 2022-04-13 Sumitomo Chemical Co Method comprising light emitting marker
GB2637891A (en) 2020-10-30 2025-08-06 Element Biosciences Inc Reagents for massively parallel nucleic acid sequencing
IL310281A (en) 2021-07-21 2024-03-01 Element Biosciences Inc Optical systems for nucleic acid sequencing and methods thereof
KR20230153706A (ko) 2022-04-29 2023-11-07 연세대학교 산학협력단 핵산의 검출 또는 분리용 조성물 및 이를 이용한 핵산의 검출 또는 분리 방법
CN116024315B (zh) * 2022-11-30 2025-09-09 温州医科大学 一种基于链置换反应的多价双锁钥系统及其在核苷酸多态性检测中的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005111240A2 (fr) * 2004-04-30 2005-11-24 Li-Cor, Inc. Séquençage à excitation coupée
WO2009073201A2 (fr) * 2007-12-04 2009-06-11 Pacific Biosciences Of California, Inc. Stratégies de marquage alternées pour séquençage de molécule unique
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2006097320A2 (fr) * 2005-03-17 2006-09-21 Genovoxx Gmbh Liaisons nucleotidiques macromoleculaires et procedes d'utilisation associes
US8993737B2 (en) * 2010-08-25 2015-03-31 Pacific Biosciences, Inc. Phospholinked dye analogs with an amino acid linker
EP2705161A1 (fr) * 2011-05-04 2014-03-12 Genovoxx GmbH Conjugués nucléoside-triphosphate et procédés d'utilisation de ceux-ci
EP3222627B1 (fr) * 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Substrats d'enzymes de polymérase avec blindage de protéine
WO2018137826A1 (fr) * 2017-01-26 2018-08-02 Qiagen Gmbh Procédé pour l'enrichissement d'acides nucléiques matriciels
CA3052297A1 (fr) * 2017-02-02 2018-08-09 Caris Science, Inc. Oligonucleotides cibles

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
WO2005111240A2 (fr) * 2004-04-30 2005-11-24 Li-Cor, Inc. Séquençage à excitation coupée
WO2009073201A2 (fr) * 2007-12-04 2009-06-11 Pacific Biosciences Of California, Inc. Stratégies de marquage alternées pour séquençage de molécule unique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2020243017A1 *

Also Published As

Publication number Publication date
DE112020002516T5 (de) 2022-03-24
JP7542000B2 (ja) 2024-08-29
KR20250096873A (ko) 2025-06-27
SG11202112049VA (en) 2021-12-30
WO2020243017A1 (fr) 2020-12-03
DE112020002516B4 (de) 2025-03-13
AU2020285657B2 (en) 2022-10-06
AU2020285657A1 (en) 2021-11-18
JP2022535187A (ja) 2022-08-05
IL301380A (en) 2023-05-01
IL287528B1 (en) 2023-04-01
GB202115667D0 (en) 2021-12-15
IL287528A (en) 2021-12-01
JP2024174880A (ja) 2024-12-17
AU2025200380A1 (en) 2025-02-06
AU2022291540A1 (en) 2023-02-02
IL316901A (en) 2025-01-01
IL301380B2 (en) 2025-05-01
KR20230165871A (ko) 2023-12-05
IL301380B1 (en) 2025-01-01
IL287528B2 (en) 2023-08-01
GB2597398A (en) 2022-01-26
AU2022291540B2 (en) 2024-11-14
KR20210144929A (ko) 2021-11-30
EP3947731A1 (fr) 2022-02-09
CA3137120A1 (fr) 2020-12-03
KR102607124B1 (ko) 2023-11-29
CN113939601A (zh) 2022-01-14
KR102823471B1 (ko) 2025-06-19
GB2597398B (en) 2024-03-06

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