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EP3893949A4 - CELL SEPARATION APPARATUS AND METHODS OF USE - Google Patents

CELL SEPARATION APPARATUS AND METHODS OF USE Download PDF

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Publication number
EP3893949A4
EP3893949A4 EP18943193.5A EP18943193A EP3893949A4 EP 3893949 A4 EP3893949 A4 EP 3893949A4 EP 18943193 A EP18943193 A EP 18943193A EP 3893949 A4 EP3893949 A4 EP 3893949A4
Authority
EP
European Patent Office
Prior art keywords
methods
separation apparatus
cell separation
cell
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP18943193.5A
Other languages
German (de)
French (fr)
Other versions
EP3893949A1 (en
Inventor
Rolf Wolters
Jules VALENTI
Tom GURSKI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koligo Therapeutics Inc
Original Assignee
Koligo Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koligo Therapeutics Inc filed Critical Koligo Therapeutics Inc
Publication of EP3893949A1 publication Critical patent/EP3893949A1/en
Publication of EP3893949A4 publication Critical patent/EP3893949A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02—Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/05—Means for pre-treatment of biological substances by centrifugation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Botany (AREA)
  • Mechanical Engineering (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Centrifugal Separators (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP18943193.5A 2018-12-13 2018-12-13 CELL SEPARATION APPARATUS AND METHODS OF USE Withdrawn EP3893949A4 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2018/065405 WO2020122916A1 (en) 2018-12-13 2018-12-13 Cell separation apparatus and methods of use

Publications (2)

Publication Number Publication Date
EP3893949A1 EP3893949A1 (en) 2021-10-20
EP3893949A4 true EP3893949A4 (en) 2022-08-17

Family

ID=71076920

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18943193.5A Withdrawn EP3893949A4 (en) 2018-12-13 2018-12-13 CELL SEPARATION APPARATUS AND METHODS OF USE

Country Status (11)

Country Link
EP (1) EP3893949A4 (en)
JP (1) JP2022522323A (en)
KR (1) KR20210092324A (en)
CN (1) CN113660961A (en)
AU (1) AU2018452704A1 (en)
BR (1) BR112021011482A2 (en)
CA (1) CA3123386A1 (en)
IL (1) IL283930A (en)
MX (1) MX2021007080A (en)
WO (1) WO2020122916A1 (en)
ZA (1) ZA202104197B (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050260175A1 (en) * 2001-12-07 2005-11-24 Hedrick Marc H Systems and methods for isolating and using clinically safe adipose derived regenerative cells
US20110111497A1 (en) * 2009-11-06 2011-05-12 Olympus Corporation Cell separation apparatus, method for activating fat-derived cells, graft material producing process, and graft material
US9144583B2 (en) * 2002-03-29 2015-09-29 Tissue Genesis, Inc. Cell separation apparatus and methods of use
KR20160006076A (en) * 2014-07-08 2016-01-18 (주)시지바이오 Container for separating stem cells from fattissue and method for separating stem cells from fattissue using the same
US20160030486A1 (en) * 2012-09-06 2016-02-04 The Gid Group, Inc. Preparation of compositions to treat and treatment of osteoarthritis using adipose-derived stromal vascular fraction cells
US20160252537A1 (en) * 2013-11-13 2016-09-01 Stempeutics Research Private Limited System and method for processing of biological tissue samples
US20180207203A1 (en) * 2010-07-09 2018-07-26 The Gid Group, Inc. Portable apparatus with mixing device and methods relating to collecting and processing human biological material comprising adipose

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035708A (en) * 1985-06-06 1991-07-30 Thomas Jefferson University Endothelial cell procurement and deposition kit
FR2729398A1 (en) * 1995-01-17 1996-07-19 Hospal R & D Int ORGAN DIGESTION DEVICE AND METHOD FOR ISOLATING CELLS OR AMAS FROM CELLS OF BIOLOGICAL INTEREST FROM AN ORGAN
US6235534B1 (en) * 1998-04-27 2001-05-22 Ronald Frederich Brookes Incremental absorbance scanning of liquid in dispensing tips
JP2002068995A (en) * 2000-08-25 2002-03-08 Phoenix Seiyaku Kk Diabetes mellitus-treating agent
US7045349B2 (en) * 2001-01-23 2006-05-16 Benedict Daniel J Method of islet isolation using process control
JP2004033006A (en) * 2002-06-28 2004-02-05 Asahi Kasei Corp Biological material digestion and cell recovery method and apparatus
AU2004260937B2 (en) * 2003-06-25 2010-05-13 Macropore Biosurgery Inc. Systems and methods for separating and concentrating regenerative cells from tissue
US7541166B2 (en) * 2003-09-19 2009-06-02 Microfluidic Systems, Inc. Sonication to selectively lyse different cell types
EP2352533B1 (en) * 2008-12-01 2018-01-10 General Electric Company System and method for separating nucleated cells from body fluids
JP2011097878A (en) * 2009-11-06 2011-05-19 Olympus Corp Cell separation apparatus
US8951513B2 (en) * 2010-12-16 2015-02-10 Ingeneron Incorporated Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples
CN103018224B (en) * 2012-12-14 2015-07-29 中国科学院上海微系统与信息技术研究所 Based on rare cells separation detecting system and the method for centrifugal microfluidic control techniques
WO2014089838A1 (en) * 2012-12-14 2014-06-19 Zheng Chong Centrifugal dynamic filtering apparatus and cell separation system using same
CA2989898C (en) * 2015-12-29 2024-01-16 Thermogenesis Corporation Cell separation devices, systems, and methods

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050260175A1 (en) * 2001-12-07 2005-11-24 Hedrick Marc H Systems and methods for isolating and using clinically safe adipose derived regenerative cells
US9144583B2 (en) * 2002-03-29 2015-09-29 Tissue Genesis, Inc. Cell separation apparatus and methods of use
US20110111497A1 (en) * 2009-11-06 2011-05-12 Olympus Corporation Cell separation apparatus, method for activating fat-derived cells, graft material producing process, and graft material
US20180207203A1 (en) * 2010-07-09 2018-07-26 The Gid Group, Inc. Portable apparatus with mixing device and methods relating to collecting and processing human biological material comprising adipose
US20160030486A1 (en) * 2012-09-06 2016-02-04 The Gid Group, Inc. Preparation of compositions to treat and treatment of osteoarthritis using adipose-derived stromal vascular fraction cells
US20160252537A1 (en) * 2013-11-13 2016-09-01 Stempeutics Research Private Limited System and method for processing of biological tissue samples
KR20160006076A (en) * 2014-07-08 2016-01-18 (주)시지바이오 Container for separating stem cells from fattissue and method for separating stem cells from fattissue using the same

Also Published As

Publication number Publication date
JP2022522323A (en) 2022-04-18
CA3123386A1 (en) 2020-06-18
CN113660961A (en) 2021-11-16
EP3893949A1 (en) 2021-10-20
ZA202104197B (en) 2023-10-25
MX2021007080A (en) 2021-10-13
KR20210092324A (en) 2021-07-23
WO2020122916A1 (en) 2020-06-18
AU2018452704A1 (en) 2021-07-29
BR112021011482A2 (en) 2021-08-31
IL283930A (en) 2021-07-29

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