EP3728581A4 - Contrôle du phénotype d'organismes avec ciblage de gène par crispr/cas - Google Patents

Contrôle du phénotype d'organismes avec ciblage de gène par crispr/cas Download PDF

Info

Publication number: EP3728581A4
Authority: EP; European Patent Office
Prior art keywords: crispr; cas; phenotype; organisms; control
Prior art date: 2017-12-22
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP18890272.0A

Other languages

German (de)

English (en)

Other versions

EP3728581B1 (fr

EP3728581A1 (fr

Inventor

Alex Michael WARD

Molly Mo-Yin LEUNG

Jean Frances SPAGNOLO-SMITH

George CHENAUX

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bio Rad Laboratories Inc

Original Assignee

Bio Rad Laboratories Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2017-12-22

Filing date

2018-12-20

Publication date

2021-09-01

2018-12-20 Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc

2020-10-28 Publication of EP3728581A1 publication Critical patent/EP3728581A1/fr

2021-09-01 Publication of EP3728581A4 publication Critical patent/EP3728581A4/fr

2024-10-09 Application granted granted Critical

2024-10-09 Publication of EP3728581B1 publication Critical patent/EP3728581B1/fr

Status Active legal-status Critical Current

2038-12-20 Anticipated expiration legal-status Critical

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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Medicinal Chemistry (AREA)
Bioinformatics & Computational Biology (AREA)
Crystallography & Structural Chemistry (AREA)
Mycology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

EP18890272.0A 2017-12-22 2018-12-20 Contrôle du phénotype d'organismes avec ciblage de gène par crispr/cas Active EP3728581B1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US201762610040P	2017-12-22	2017-12-22
PCT/US2018/066908 WO2019126562A1 (fr)	2017-12-22	2018-12-20	Contrôle du phénotype d'organismes avec ciblage de gène par crispr/cas

Publications (3)

Publication Number	Publication Date
EP3728581A1 EP3728581A1 (fr)	2020-10-28
EP3728581A4 true EP3728581A4 (fr)	2021-09-01
EP3728581B1 EP3728581B1 (fr)	2024-10-09

Family

ID=66993851

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP18890272.0A Active EP3728581B1 (fr)	2017-12-22	2018-12-20	Contrôle du phénotype d'organismes avec ciblage de gène par crispr/cas

Country Status (4)

Country	Link
US (1)	US20200318106A1 (fr)
EP (1)	EP3728581B1 (fr)
CN (1)	CN111566208A (fr)
WO (1)	WO2019126562A1 (fr)

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US12181452B2 (en)	2017-09-18	2024-12-31	Waters Technologies Corporation	Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes
US11709156B2 (en)	2017-09-18	2023-07-25	Waters Technologies Corporation	Use of vapor deposition coated flow paths for improved analytical analysis
US12180581B2 (en)	2017-09-18	2024-12-31	Waters Technologies Corporation	Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes
US11709155B2 (en)	2017-09-18	2023-07-25	Waters Technologies Corporation	Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes
MX2021012157A (es) *	2019-04-05	2022-01-06	Danisco Us Inc	Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos.
WO2020245093A1 (fr) *	2019-06-02	2020-12-10	Redbiotec Ag	Administration bactérienne d'outils d'édition de gènes dans des cellules eucaryotes
CN111235130B (zh) *	2019-11-15	2022-11-25	武汉大学	II类V型CRISPR蛋白CeCas12a及其在基因编辑的应用
US11918936B2 (en)	2020-01-17	2024-03-05	Waters Technologies Corporation	Performance and dynamic range for oligonucleotide bioanalysis through reduction of non specific binding
CN114231547A (zh) *	2020-09-09	2022-03-25	中国科学院微生物研究所	CRISPR/Cas9系统的一种新应用
CN116391122A (zh)	2020-09-24	2023-07-04	沃特世科技公司	用于反应性分子分离的色谱硬件改进
US10947552B1 (en)	2020-09-30	2021-03-16	Alpine Roads, Inc.	Recombinant fusion proteins for producing milk proteins in plants
US10894812B1 (en)	2020-09-30	2021-01-19	Alpine Roads, Inc.	Recombinant milk proteins
EP4222167A1 (fr)	2020-09-30	2023-08-09	Nobell Foods, Inc.	Protéines de lait recombinantes et compositions les comprenant
AU2022245134A1 (en) *	2021-03-24	2023-09-14	Syngenta Crop Protection Ag	Inducible mosaicism
CN116949085B (zh) *	2023-09-19	2023-12-22	广东省农业科学院蔬菜研究所	草菇Vvpks基因在调控开伞性状中的应用
CN118460446B (zh) *	2024-07-12	2024-11-15	内蒙古自治区农牧业科学院	一种支原体突变株及其制备方法与应用

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WO2016011080A2 (fr) *	2014-07-14	2016-01-21	The Regents Of The University Of California	Modulation transcriptionnelle par crispr/cas

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US10287590B2 (en) *	2014-02-12	2019-05-14	Dna2.0, Inc.	Methods for generating libraries with co-varying regions of polynuleotides for genome modification
KR102243243B1 (ko) *	2014-10-23	2021-04-22	리제너론 파마슈티칼스 인코포레이티드	신규한 cho 통합 부위 및 이의 용도
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WO2017009399A1 (fr) *	2015-07-13	2017-01-19	Institut Pasteur	Amélioration des agents antimicrobiens spécifiques à une séquence par le blocage de la réparation de l'adn
US9580727B1 (en) *	2015-08-07	2017-02-28	Caribou Biosciences, Inc.	Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
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EP3312192B1 (fr) *	2016-10-24	2023-02-22	BiOMVis Srl	Compositions immunogènes contenant des vésicules de membrane externe de bactéries
WO2018148410A1 (fr) *	2017-02-09	2018-08-16	The Charles Stark Draper Laboratory Inc.	Recombinaison de bactériophages suivie du blocage de la réplication de bactériophages non recombinés

2018
- 2018-12-20 EP EP18890272.0A patent/EP3728581B1/fr active Active
- 2018-12-20 US US16/955,682 patent/US20200318106A1/en active Pending
- 2018-12-20 WO PCT/US2018/066908 patent/WO2019126562A1/fr not_active Ceased
- 2018-12-20 CN CN201880083194.2A patent/CN111566208A/zh active Pending

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WO2015017866A1 (fr) *	2013-08-02	2015-02-05	Enevolv, Inc.	Procédés et cellules hôtes pour ingénierie biomoléculaire, génomique et de la voie
WO2016011080A2 (fr) *	2014-07-14	2016-01-21	The Regents Of The University Of California	Modulation transcriptionnelle par crispr/cas

Also Published As

Publication number	Publication date
WO2019126562A1 (fr)	2019-06-27
EP3728581B1 (fr)	2024-10-09
EP3728581A1 (fr)	2020-10-28
CN111566208A (zh)	2020-08-21
US20200318106A1 (en)	2020-10-08

Publication	Publication Date	Title
EP3728581A4 (fr)	2021-09-01	Contrôle du phénotype d'organismes avec ciblage de gène par crispr/cas
EP3612181A4 (fr)	2021-01-06	Thérapies d'association avec des inhibiteurs d'ehmt2
IL281930A (en)	2021-05-31	Rna particles comprising polysarcosin
EP3655836A4 (fr)	2021-04-21	Repositionnement d'un véhicule autonome
IL271389A (en)	2020-01-30	Targeted non-viral dna insertions
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IL281223A (en)	2021-04-29	The history of heterocycles as pesticides
EP3651775A4 (fr)	2021-04-07	Méthodes d'inhibition de l'expression génique d'hao1 (hydroxyacide oxydase 1 (glycolate oxydase))
IL260148A (en)	2018-07-31	Heterocyclene derivatives as pest control agents
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Sherwood et al.	2018	Are we wired differently?
MA53225A (fr)	2021-06-23	Agents inhibiteurs d'ask1
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EP3720866A4 (fr)	2021-09-08	Lutte contre des organismes nuisibles aux plantes à l'aide de molécules d'arn

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