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EP3669163A4 - Devices and methods for separating circulating tumor cells from biological samples - Google Patents

Devices and methods for separating circulating tumor cells from biological samples Download PDF

Info

Publication number
EP3669163A4
EP3669163A4 EP18841411.4A EP18841411A EP3669163A4 EP 3669163 A4 EP3669163 A4 EP 3669163A4 EP 18841411 A EP18841411 A EP 18841411A EP 3669163 A4 EP3669163 A4 EP 3669163A4
Authority
EP
European Patent Office
Prior art keywords
methods
devices
tumor cells
biological samples
circulating tumor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18841411.4A
Other languages
German (de)
French (fr)
Other versions
EP3669163A1 (en
Inventor
Leidong Mao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Georgia
University of Georgia Research Foundation Inc UGARF
Original Assignee
University of Georgia
University of Georgia Research Foundation Inc UGARF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Georgia, University of Georgia Research Foundation Inc UGARF filed Critical University of Georgia
Publication of EP3669163A1 publication Critical patent/EP3669163A1/en
Publication of EP3669163A4 publication Critical patent/EP3669163A4/en
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP18841411.4A 2017-08-04 2018-08-04 Devices and methods for separating circulating tumor cells from biological samples Pending EP3669163A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762541552P 2017-08-04 2017-08-04
PCT/US2018/045294 WO2019028445A1 (en) 2017-08-04 2018-08-04 Devices and methods for separating circulating tumor cells from biological samples

Publications (2)

Publication Number Publication Date
EP3669163A1 EP3669163A1 (en) 2020-06-24
EP3669163A4 true EP3669163A4 (en) 2021-08-11

Family

ID=65233143

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18841411.4A Pending EP3669163A4 (en) 2017-08-04 2018-08-04 Devices and methods for separating circulating tumor cells from biological samples

Country Status (3)

Country Link
EP (1) EP3669163A4 (en)
CA (1) CA3071593A1 (en)
WO (1) WO2019028445A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110093246B (en) * 2019-05-05 2022-05-24 温州医科大学 Multi-pole magnetic field continuous magnetic bead cell separation device and method thereof
US20240094106A1 (en) * 2021-02-03 2024-03-21 University Of Georgia Research Foundation, Inc. Devices, kits, and methods for label-free inertial ferrohydrodynamic cell separation with high throughput and resolution

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432630B1 (en) * 1996-09-04 2002-08-13 Scandinanian Micro Biodevices A/S Micro-flow system for particle separation and analysis
DE102009005925A1 (en) * 2009-01-23 2010-07-29 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Device for handling bio molecules to accomplish immunoassay, has rotary encoder producing relative motion between magnetic force element and channel structure for moving magnetic particles over phase boundary of multiphase flow
US20100297733A1 (en) * 2009-04-21 2010-11-25 Qiao Lin Systems And Methods For The Capture And Separation Of Microparticles
US20130306566A1 (en) * 2012-05-18 2013-11-21 University Of Georgia Research Foundation, Inc. Devices and methods for separating particles
US9278353B2 (en) * 2012-06-25 2016-03-08 The General Hospital Corporation Sorting particles using high gradient magnetic fields
US20160244714A1 (en) * 2013-10-18 2016-08-25 The General Hosptial Corporation Microfluidic sorting using high gradient magnetic fields
US20170029782A1 (en) * 2015-07-31 2017-02-02 University Of Georgia Research Foundation, Inc. Devices and methods for separating particles

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010117458A1 (en) * 2009-04-10 2010-10-14 President And Fellows Of Harvard College Manipulation of particles in channels
JP6757723B2 (en) * 2014-11-03 2020-09-23 ザ ジェネラル ホスピタル コーポレイション Particle enrichment in microfluidic equipment

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432630B1 (en) * 1996-09-04 2002-08-13 Scandinanian Micro Biodevices A/S Micro-flow system for particle separation and analysis
DE102009005925A1 (en) * 2009-01-23 2010-07-29 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Device for handling bio molecules to accomplish immunoassay, has rotary encoder producing relative motion between magnetic force element and channel structure for moving magnetic particles over phase boundary of multiphase flow
US20100297733A1 (en) * 2009-04-21 2010-11-25 Qiao Lin Systems And Methods For The Capture And Separation Of Microparticles
US20130306566A1 (en) * 2012-05-18 2013-11-21 University Of Georgia Research Foundation, Inc. Devices and methods for separating particles
US9278353B2 (en) * 2012-06-25 2016-03-08 The General Hospital Corporation Sorting particles using high gradient magnetic fields
US20160244714A1 (en) * 2013-10-18 2016-08-25 The General Hosptial Corporation Microfluidic sorting using high gradient magnetic fields
US20170029782A1 (en) * 2015-07-31 2017-02-02 University Of Georgia Research Foundation, Inc. Devices and methods for separating particles

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2019028445A1 *

Also Published As

Publication number Publication date
EP3669163A1 (en) 2020-06-24
CA3071593A1 (en) 2019-02-07
WO2019028445A1 (en) 2019-02-07

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