EP3585903C0 - PRODUCTION OF CAROTENOIDS AND APOCAROTENOIDS - Google Patents

PRODUCTION OF CAROTENOIDS AND APOCAROTENOIDS

Info

Publication number: EP3585903C0
Authority: EP; European Patent Office
Prior art keywords: apocarotenoids; carotenoids; production
Prior art date: 2017-02-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Active

Application number

EP18758451.1A

Other languages

German (de)

French (fr)

Other versions

EP3585903B1 (en

EP3585903A4 (en

EP3585903A1 (en

Inventor

Congqiang Zhang

Xixian Chen

Heng-Phon Too

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Agency for Science Technology and Research Singapore

Original Assignee

Agency for Science Technology and Research Singapore

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2017-02-24

Filing date

2018-02-26

Publication date

2024-04-17

2018-02-26 Application filed by Agency for Science Technology and Research Singapore filed Critical Agency for Science Technology and Research Singapore

2018-02-26 Priority to EP24165181.9A priority Critical patent/EP4403627A3/en

2020-01-01 Publication of EP3585903A1 publication Critical patent/EP3585903A1/en

2021-05-19 Publication of EP3585903A4 publication Critical patent/EP3585903A4/en

2024-04-17 Application granted granted Critical

2024-04-17 Publication of EP3585903B1 publication Critical patent/EP3585903B1/en

2024-04-17 Publication of EP3585903C0 publication Critical patent/EP3585903C0/en

Status Active legal-status Critical Current

2038-02-26 Anticipated expiration legal-status Critical

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
- C12Y113/11051—9-Cis-epoxycarotenoid dioxygenase (1.13.11.51)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
- C12Y113/11063—Beta-carotene 15,15'-dioxygenase (1.13.11.63)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
- C12Y113/11068—9-Cis-beta-carotene 9',10'-cleaving dioxygenase (1.13.11.68)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01018—Lycopene epsilon-cyclase (5.5.1.18)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01019—Lycopene beta-cyclase (5.5.1.19)

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Chemical Kinetics & Catalysis (AREA)
Plant Pathology (AREA)
General Chemical & Material Sciences (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

EP18758451.1A 2017-02-24 2018-02-26 Production of carotenoids and apocarotenoids Active EP3585903B1 (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
EP24165181.9A EP4403627A3 (en)	2017-02-24	2018-02-26	Production of carotenoids and apocarotenoids

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
SG10201701500U		2017-02-24
PCT/SG2018/050087 WO2018156086A1 (en)	2017-02-24	2018-02-26	Production of carotenoids and apocarotenoids

Related Child Applications (1)

Application Number	Title	Priority Date	Filing Date
EP24165181.9A Division EP4403627A3 (en)	2017-02-24	2018-02-26	Production of carotenoids and apocarotenoids

Publications (4)

Publication Number	Publication Date
EP3585903A1 EP3585903A1 (en)	2020-01-01
EP3585903A4 EP3585903A4 (en)	2021-05-19
EP3585903B1 EP3585903B1 (en)	2024-04-17
EP3585903C0 true EP3585903C0 (en)	2024-04-17

Family

ID=63252286

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
EP18758451.1A Active EP3585903B1 (en)	2017-02-24	2018-02-26	Production of carotenoids and apocarotenoids
EP24165181.9A Pending EP4403627A3 (en)	2017-02-24	2018-02-26	Production of carotenoids and apocarotenoids

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
EP24165181.9A Pending EP4403627A3 (en)	2017-02-24	2018-02-26	Production of carotenoids and apocarotenoids

Country Status (6)

Country	Link
US (2)	US11920137B2 (en)
EP (2)	EP3585903B1 (en)
CN (2)	CN110546268B (en)
ES (1)	ES2985126T3 (en)
SG (2)	SG11201907812TA (en)
WO (1)	WO2018156086A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US11124809B2 (en) *	2017-12-21	2021-09-21	Centrome, Inc.	Production of alpha-(R)-(E)-(+)-ionone in recombinant Saccharomyces cerevisiae
KR20210018056A (en)	2019-08-08	2021-02-17	에이전시 포 사이언스, 테크놀로지 앤드 리서치	Viridiflorol Production In Auxotrophic Escherichia Coli
CN111100849B (en) *	2020-01-16	2021-01-12	安徽农业大学	Tea tree carotenoid cleavage dioxygenase CsCCD4 and its application in catalytic synthesis of β-ionone
EP4140293A1 (en) *	2020-04-21	2023-03-01	Japan Tobacco Inc.	Tobacco plant body and method for producing same
WO2022245301A2 (en) *	2021-05-21	2022-11-24	Agency For Science, Technology And Research	De novo biosynthesis of glycosylated carotenoids
CN113549639B (en) *	2021-07-21	2022-07-29	云南中烟工业有限责任公司	Regulatory gene for reducing contents of total proteins and phenol in flue gas of tobacco leaves
EP4486906A2 (en) *	2022-03-01	2025-01-08	Agency for Science, Technology and Research	Biosynthesis of apocarotenoids by controlling oxidative stress
CN116064433B (en) *	2022-09-19	2025-09-16	云南大学	Carotenoid dioxygenase mutant and application thereof
WO2024163976A1 (en) *	2023-02-03	2024-08-08	Amyris, Inc.	Host cells capable of producing retinol or retinol precursors and methods of use thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20030166595A1 (en)	2000-03-20	2003-09-04	Johannes Von Lintig	Novel deoxygenases catalyzing cleavage of beta-carotene
US7252964B2 (en)	2001-06-12	2007-08-07	Institut De Recherche Pour Le Developpement (I.R.D.)	Isolated carotenoid biosynthesis gene cluster involved in canthaxanthin production and applications thereof
US20040219629A1 (en)	2002-12-19	2004-11-04	Qiong Cheng	Increasing carotenoid production in bacteria via chromosomal integration
AU2006287233A1 (en)	2005-09-02	2007-03-08	Cornell University	Polynucleotides encoding carotenoid and apocarotenoid biosynthetic pathway
GB0524873D0 (en) *	2005-12-06	2006-01-11	New Royal Holloway & Bedford	Bacterial production of carotenoids
US20090233342A1 (en) *	2007-08-23	2009-09-17	Asuncion Martinez	Heterologous expression of proteorhodopsin photosystem
KR101169739B1 (en)	2010-12-08	2012-08-02	한국생명공학연구원	Donghaeana dokdonensis blh having enhanced in enzyme activity for the production of vitamin A
WO2013019051A2 (en) *	2011-07-29	2013-02-07	경상대학교산학협력단	Method for producing retinoid from microorganism
US9644217B2 (en)	2011-07-29	2017-05-09	Industry-Academic Cooperation Foundation Gyeongsang National University	Method for producing retinoid from microorganism
CN102321649B (en)	2011-09-22	2013-11-13	天津大学	Lycium chinense miller lycopene beta-cyclase gene, recombinant vector containing gene, host cell and application
US10364434B2 (en) *	2015-03-23	2019-07-30	Arch Innotek, Llc	Compositions and methods of biosynthesizing carotenoids and their derivatives
ES2882077T3 (en)	2015-08-28	2021-12-01	Phytowelt Greentechnologies Gmbh	Procedure for the fermentative production of alpha-ionone
KR101724992B1 (en)	2016-01-27	2017-04-11	경상대학교산학협력단	Microorganism including genes coding enzymes involved in the production of retinoid and preparing method for retinoid using the same

2018
- 2018-02-26 EP EP18758451.1A patent/EP3585903B1/en active Active
- 2018-02-26 CN CN201880026537.1A patent/CN110546268B/en active Active
- 2018-02-26 SG SG11201907812TA patent/SG11201907812TA/en unknown
- 2018-02-26 US US16/488,504 patent/US11920137B2/en active Active
- 2018-02-26 CN CN202311292626.XA patent/CN117143951A/en active Pending
- 2018-02-26 ES ES18758451T patent/ES2985126T3/en active Active
- 2018-02-26 WO PCT/SG2018/050087 patent/WO2018156086A1/en not_active Ceased
- 2018-02-26 SG SG10202004779XA patent/SG10202004779XA/en unknown
- 2018-02-26 EP EP24165181.9A patent/EP4403627A3/en active Pending
2023
- 2023-10-26 US US18/384,044 patent/US12378561B2/en active Active

Also Published As

Publication number	Publication date
ES2985126T3 (en)	2024-11-04
SG11201907812TA (en)	2019-09-27
CN110546268A (en)	2019-12-06
CN117143951A (en)	2023-12-01
US12378561B2 (en)	2025-08-05
EP3585903B1 (en)	2024-04-17
US20190367928A1 (en)	2019-12-05
EP3585903A4 (en)	2021-05-19
US11920137B2 (en)	2024-03-05
US20240175036A1 (en)	2024-05-30
CN110546268B (en)	2023-10-20
WO2018156086A1 (en)	2018-08-30
EP3585903A1 (en)	2020-01-01
EP4403627A2 (en)	2024-07-24
EP4403627A3 (en)	2024-10-16
SG10202004779XA (en)	2020-06-29

Publication	Publication Date	Title
EP3585903A4 (en)	2021-05-19	PRODUCTION OF CAROTENOIDS AND APOCAROTENOIDS
EP4059510C0 (en)	2025-05-21	MANIPULATED EXTRACELLULAR VESILES AND USES THEREOF
EP3678681A4 (en)	2021-07-14	INTERLEUKIN-18 VARIANTS AND METHODS OF USE
EP3328363A4 (en)	2019-08-21	COMPOSITIONS AND METHODS OF IMMUNOMODULATION
MA49847A (en)	2021-06-02	ENZYMATIC PRODUCTION OF HEXOSES
EP3641787A4 (en)	2021-03-17	PRODUCTION OF BIOACTIVE OLIGOSACCHARIDES
PT3317260T (en)	2020-02-06	COMPOSITIONS OF POTASSIUM ACESSULFAME AND PRODUCTION PROCESSES OF THE SAME
PT3368534T (en)	2021-03-09	VALBENAZINE DITHOSILATE AND POLYMORPH OF THE SAME
EP3512988A4 (en)	2020-05-27	PRODUCTION OF GRAPHS
EP3341485A4 (en)	2019-03-27	ENHANCED PRODUCTION OF IMMUNOGLOBULINS
EP3647305A4 (en)	2020-07-01	PIPERIDIC ACID DERIVATIVE AND PRODUCTION AND APPLICATION THEREOF
IL285784A (en)	2021-09-30	Graphene and production of graphene
SG11202004381WA (en)	2020-06-29	Production of guaiene and rotundone
IL258010B (en)	2020-04-30	Perfume material of green lily of the valley
EP3446589A4 (en)	2019-11-20	PERSONAL ORNAMENT
EP3672640A4 (en)	2021-04-21	PROBIOTIC-LOADED MICROCAPSULES AND THEIR PRODUCTION
EP3719114A4 (en)	2021-08-18	NOVEL MICROALGAE AND USES THEREOF
EP3357485A4 (en)	2019-04-10	COSMETICS OF SOLAR SCREEN
EP3799594A4 (en)	2022-06-15	PRODUCTION OF OLIGOSACCHARIDES
EP3604782A4 (en)	2020-12-23	SLIDER AND PISTON RING
EP3506254A4 (en)	2019-11-27	STRUCTURE OF SOUNDS
EP3405024A4 (en)	2019-07-03	PARTHENOCARPIC PLANTS AND METHODS OF PRODUCTION THEREOF
PL3446187T3 (en)	2020-11-02	Manner of operation of the production installation and production installation
EP3663430A4 (en)	2020-12-09	SLIDER AND PISTON RING
EP3624597C0 (en)	2023-10-04	LEGUME-BASED PASTA AND PRODUCTION THEREOF

Legal Events

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2020-12-30	RIC1	Information provided on ipc code assigned before grant	Ipc: C12N 9/02 20060101ALI20201124BHEP Ipc: C12P 23/00 20060101AFI20201124BHEP Ipc: C12P 5/00 20060101ALI20201124BHEP Ipc: C12N 15/52 20060101ALI20201124BHEP Ipc: C12N 9/90 20060101ALI20201124BHEP Ipc: C07K 14/245 20060101ALI20201124BHEP
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