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EP3583214A4 - PROCEDURES AND COMPOSITIONS FOR IDENTIFICATION OR QUANTIFICATION OF TARGETS IN A BIOLOGICAL SAMPLE - Google Patents

PROCEDURES AND COMPOSITIONS FOR IDENTIFICATION OR QUANTIFICATION OF TARGETS IN A BIOLOGICAL SAMPLE Download PDF

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Publication number
EP3583214A4
EP3583214A4 EP18747697.3A EP18747697A EP3583214A4 EP 3583214 A4 EP3583214 A4 EP 3583214A4 EP 18747697 A EP18747697 A EP 18747697A EP 3583214 A4 EP3583214 A4 EP 3583214A4
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EP
European Patent Office
Prior art keywords
quantification
targets
procedures
compositions
identification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP18747697.3A
Other languages
German (de)
French (fr)
Other versions
EP3583214B1 (en
EP3583214A1 (en
Inventor
Marlon STOECKIUS
Peter SMIBERT
Brian HOUCK-LOOMIS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New York Genome Center Inc
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New York Genome Center Inc
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Publication date
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Priority to EP23203416.5A priority Critical patent/EP4324931A3/en
Publication of EP3583214A1 publication Critical patent/EP3583214A1/en
Publication of EP3583214A4 publication Critical patent/EP3583214A4/en
Application granted granted Critical
Publication of EP3583214B1 publication Critical patent/EP3583214B1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP18747697.3A 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample Active EP3583214B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP23203416.5A EP4324931A3 (en) 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762453726P 2017-02-02 2017-02-02
US201762515180P 2017-06-05 2017-06-05
US201762549189P 2017-08-23 2017-08-23
US201762559228P 2017-09-15 2017-09-15
US201762599450P 2017-12-15 2017-12-15
US201762609332P 2017-12-21 2017-12-21
PCT/US2018/016587 WO2018144813A1 (en) 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP23203416.5A Division-Into EP4324931A3 (en) 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample
EP23203416.5A Division EP4324931A3 (en) 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample

Publications (3)

Publication Number Publication Date
EP3583214A1 EP3583214A1 (en) 2019-12-25
EP3583214A4 true EP3583214A4 (en) 2020-12-23
EP3583214B1 EP3583214B1 (en) 2023-11-22

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Family Applications (2)

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EP18747697.3A Active EP3583214B1 (en) 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample
EP23203416.5A Pending EP4324931A3 (en) 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample

Family Applications After (1)

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EP23203416.5A Pending EP4324931A3 (en) 2017-02-02 2018-02-02 Methods and compositions for identifying or quantifying targets in a biological sample

Country Status (7)

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US (3) US20180251825A1 (en)
EP (2) EP3583214B1 (en)
CN (2) CN117887804A (en)
DK (1) DK3583214T5 (en)
ES (1) ES2968290T3 (en)
FI (1) FI3583214T3 (en)
WO (1) WO2018144813A1 (en)

Families Citing this family (124)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
AU2013226081B2 (en) 2012-02-27 2018-06-14 Becton, Dickinson And Company Compositions and kits for molecular counting
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
WO2015200893A2 (en) * 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
WO2016162309A1 (en) 2015-04-10 2016-10-13 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
US12123878B2 (en) 2016-05-02 2024-10-22 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
CA3019589A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018156553A1 (en) * 2017-02-21 2018-08-30 Arizona Board Of Regents On Behalf Of Arizona State University A method for targeted protein quantification by bar-coding affinity reagents with unique dna sequences
KR102790050B1 (en) * 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 Sample indexing for single cells
CA3081451A1 (en) 2017-10-31 2019-05-09 Encodia, Inc. A method for assaying interactions between biological targets and binding agents
EP3704249B1 (en) 2017-10-31 2025-05-21 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
US11332736B2 (en) * 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
EP3728636B1 (en) 2017-12-19 2024-09-11 Becton, Dickinson and Company Particles associated with oligonucleotides
CN112005115A (en) 2018-02-12 2020-11-27 10X基因组学有限公司 Methods to characterize multiple analytes from single cells or cell populations
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
ES3014208T3 (en) 2018-05-03 2025-04-21 Becton Dickinson Co Molecular barcoding on opposite transcript ends
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11414699B2 (en) * 2018-05-15 2022-08-16 Mantra Bio, Inc. Barcode-free single vesicle multiplexed protein and RNA analysis
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN118853827A (en) 2018-10-01 2024-10-29 贝克顿迪金森公司 Determine 5' transcript sequence
JP7618548B2 (en) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー Whole-transcriptome analysis of single cells using random priming
CN113227395A (en) * 2018-12-04 2021-08-06 豪夫迈·罗氏有限公司 Spatially directed quantum barcoding of cellular targets
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
US20220062394A1 (en) 2018-12-17 2022-03-03 The Broad Institute, Inc. Methods for identifying neoantigens
US12169198B2 (en) * 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11371076B2 (en) * 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322B1 (en) * 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020159757A1 (en) * 2019-01-28 2020-08-06 Becton, Dickinson And Company Oligonucleotide-comprising cellular component-binding reagents and methods of using the same
WO2020163789A1 (en) * 2019-02-08 2020-08-13 The Trustees Of Columbia University In The City Of New York Single cell/exosome/vesicle protein profiling
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
MX2021003746A (en) * 2019-03-01 2021-06-23 Illumina Inc High-throughput single-nuclei and single-cell libraries and methods of making and of using.
JP2022522220A (en) * 2019-04-02 2022-04-14 ミッション バイオ インコーポレイテッド Methods and systems for proteomics profiling and characterization
US20220205035A1 (en) * 2019-04-05 2022-06-30 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CA3138367A1 (en) 2019-04-30 2020-11-05 Encodia, Inc. Methods for preparing analytes and related kits
EP3973074A4 (en) 2019-05-22 2023-09-06 Mission Bio, Inc. METHOD AND DEVICE FOR SIMULTANEOUS TARGETED SEQUENCING OF DNA, RNA AND PROTEIN
JP7511257B2 (en) * 2019-07-11 2024-07-05 学校法人東京理科大学 Nucleic acid amplification method using a solid phase carrier
CN114051534B (en) 2019-07-22 2025-02-21 贝克顿迪金森公司 Single-cell chromatin immunoprecipitation sequencing assay
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
CN114729400A (en) * 2019-09-23 2022-07-08 元素生物科学公司 Methods for cell-addressable nucleic acid sequencing
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
WO2021067162A1 (en) * 2019-09-30 2021-04-08 The General Hospital Corporation Droplet-based single extracellular vesicle sequencing
IL291859A (en) 2019-10-03 2022-06-01 Regeneron Pharma High-throughput method to screen cognate t cell and epitope reactivities in primary human cells
CN112176419B (en) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 Method for detecting variation and methylation of tumor specific genes in ctDNA
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
WO2021097250A2 (en) 2019-11-14 2021-05-20 The Trustees Of Columbia University In The City Of New York Systems, methods, and compositions for generating multi-omic information from single cells
IT201900024159A1 (en) 2019-12-16 2021-06-16 Menarini Silicon Biosystems Spa METHOD AND KIT FOR TOTAL AMPLIFICATION OF THE GENOME AND ANALYSIS OF TARGET MOLECULES IN A BIOLOGICAL SAMPLE
JP7766029B2 (en) * 2019-12-20 2025-11-07 キージーン ナムローゼ フェンノートシャップ NGS library preparation using covalently closed nucleic acid molecule ends
CN115038794A (en) 2019-12-23 2022-09-09 10X基因组学有限公司 Compositions and methods for using fixed biological samples in partition-based assays
WO2021141138A1 (en) * 2020-01-10 2021-07-15 シンクサイト株式会社 Novel cellular phenotype screening method
US20210214770A1 (en) * 2020-01-13 2021-07-15 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
EP4090763B1 (en) * 2020-01-13 2024-12-04 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US12416037B2 (en) 2020-01-24 2025-09-16 Vanderbilt University Methods for identification of ligand-blocking antibodies and for determining antibody potency
US20210230681A1 (en) * 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
EP4471155A3 (en) 2020-01-29 2024-12-18 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN115087746A (en) * 2020-02-12 2022-09-20 贝克顿迪金森公司 Intracellular AbSeq
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
EP4114965A1 (en) * 2020-03-05 2023-01-11 Biolegend, Inc. Methods and compositions for detecting targets
EP4121016A4 (en) * 2020-03-16 2024-09-11 Fluent Biosciences Inc. MULTIOMIC ANALYSIS IN MONODISPERSE DROPLETS
EP4121552A4 (en) * 2020-03-18 2024-04-03 CZ Biohub SF, LLC SEQUENCING BY SINGLE-CELL COMBINATORIAL INDEXED CYTOMETRY
CN111370058B (en) * 2020-03-19 2022-12-06 佛山科学技术学院 A method for tracing the source of buffalo ancestry and performing genome matching based on genome-wide SNP information
CN111440846B (en) * 2020-04-09 2020-12-18 江苏先声医学诊断有限公司 Position anchoring bar code system for nanopore sequencing library building
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
JPWO2022009642A1 (en) * 2020-07-06 2022-01-13
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4182328B1 (en) * 2020-07-17 2025-09-24 13.8, Inc. Methods, compositions, and systems for mapping locations of single molecules in multi-dimensional space
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
EP3950955A1 (en) 2020-08-06 2022-02-09 Albert-Ludwigs-Universität Freiburg Multianalyte assay for the simultaneous detection of nucleic acid and analytes
US20220090089A1 (en) * 2020-09-24 2022-03-24 The Broad Institute, Inc. Engineered multi-omic display constructs and display systems
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
US20230257801A1 (en) 2020-11-23 2023-08-17 Pleno, Inc. Encoded Dual-Probe Endonuclease Assays
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
US20240042442A1 (en) * 2020-12-22 2024-02-08 Hifibio Sas Microfluidic methods and systems
AU2022227563A1 (en) * 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
JP2024509984A (en) * 2021-03-19 2024-03-05 パリ シアンス エ レットル Method for selective analysis of cells or organelles
WO2023023584A2 (en) 2021-08-19 2023-02-23 Eclipse Bioinnovations, Inc. Methods for detecting rna binding protein complexes
AU2022380841A1 (en) 2021-11-04 2024-05-02 Regeneron Pharmaceuticals, Inc. Viral particles retargeted to skeletal muscle
WO2023114812A1 (en) * 2021-12-15 2023-06-22 BioLegend, Inc. Compositions and methods for enhanced sample multiplexing
US20250066852A1 (en) * 2021-12-22 2025-02-27 St. Jude Children's Research Hospital, Inc. Kit and Method for Analyzing T Cell Receptors from Single T Cells
CN114252602B (en) * 2021-12-22 2023-09-12 清华大学深圳国际研究生院 Microfluidic chip, detection system based on microfluidic chip and detection method of bacteria
WO2023129973A2 (en) * 2021-12-30 2023-07-06 Eclipse Bioinnovations, Inc. Methods for detecting rna modification targets on a gene
CN118974251A (en) * 2022-01-28 2024-11-15 西湖大学 Conjugate of nucleic acid or its derivative and cell, preparation method and use thereof
WO2023172977A1 (en) * 2022-03-09 2023-09-14 Becton, Dickinson And Company Modified flow proxy assay prior to single-cell cite-seq
WO2024015952A1 (en) * 2022-07-15 2024-01-18 Seq Biomarque, Llc Methods for identifying and quantifying antigens in a sample
CN119301155A (en) 2022-07-19 2025-01-10 百进生物科技公司 Anti-CD157 antibodies, antigen-binding fragments thereof, and compositions thereof, and methods of preparing and using the same
WO2024040114A2 (en) 2022-08-18 2024-02-22 BioLegend, Inc. Anti-axl antibodies, antigen-binding fragments thereof and methods for making and using the same
CN116068198B (en) * 2022-11-30 2024-01-09 深圳湾实验室 PPI in-situ detection method and carrier, diagnostic reagent, kit and application thereof
IL322397A (en) 2023-02-13 2025-09-01 Regeneron Pharma Treatment of muscle related disorders with anti-human cacng1 antibodies
CN116024307B (en) * 2023-02-20 2023-08-11 北京寻因生物科技有限公司 A single-cell library construction method and sequencing method containing tissue location information
CN121039293A (en) * 2023-02-28 2025-11-28 俄勒冈健康与科学大学 Compositions and methods for molecular barcoding
US20240309357A1 (en) * 2023-03-13 2024-09-19 California Institute Of Technology Methods and compositions for quantitative, high-throughput protein binding measurement
WO2024243217A1 (en) 2023-05-25 2024-11-28 BioLegend, Inc. Ceacam6 binding antibodies and antigen-binding fragments thereof
WO2024243511A1 (en) 2023-05-25 2024-11-28 Regeneron Pharmaceuticals, Inc. T cell receptors that bind presented hpv16-, mart1-, cmv-, ebv-, or influenza- peptides
WO2025006955A2 (en) * 2023-06-30 2025-01-02 Ionis Pharmaceuticals, Inc. Cd29 targeted oligonucleotides and uses thereof
WO2025072313A1 (en) 2023-09-27 2025-04-03 BioLegend, Inc. Anti-gpc4 antibodies
CN119920311A (en) * 2025-01-24 2025-05-02 广州国家实验室 A method and system for single cell data quality control processing

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150376609A1 (en) * 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
WO2016040476A1 (en) * 2014-09-09 2016-03-17 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110171749A1 (en) * 2009-03-02 2011-07-14 Board Of Trustees Of Michigan State University Nanoparticle tracer-based electrochemical dna sensor for detection of pathogens-amplification by a universal nano-tracer (aunt)
CN103703143B (en) 2011-01-31 2016-12-14 爱普瑞斯生物公司 Method for identifying multiple epitopes in a cell
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014047561A1 (en) 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
CN107614700A (en) 2015-03-11 2018-01-19 布罗德研究所有限公司 genotype and phenotype coupling

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150376609A1 (en) * 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
WO2016040476A1 (en) * 2014-09-09 2016-03-17 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis

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