EP3486321A4 - T-CELL RECEPTOR AND B-CELL RECEPTOR REPERTOIRE ANALYSIS SYSTEM USING A TEMPLATE-SWITCHING POLYMERASE CHAIN RACTION WITH ONE-STAGE REVERSE TRANSCRIPTION - Google Patents

T-CELL RECEPTOR AND B-CELL RECEPTOR REPERTOIRE ANALYSIS SYSTEM USING A TEMPLATE-SWITCHING POLYMERASE CHAIN RACTION WITH ONE-STAGE REVERSE TRANSCRIPTION Download PDF

Info

Publication number: EP3486321A4
Authority: EP; European Patent Office
Prior art keywords: cell receptor; raction; template; analysis system; polymerase chain
Prior art date: 2016-06-23
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP17815521.4A

Other languages

German (de)

French (fr)

Other versions

EP3486321A1 (en

Inventor

Katsuyuki Shiroguchi

Ryuji Suzuki

Takaji Matsutani

Kazutaka Kitaura

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Repertoire Genesis Inc

RIKEN

Original Assignee

Repertoire Genesis Inc

RIKEN

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2016-06-23

Filing date

2017-06-23

Publication date

2020-01-15

2017-06-23 Application filed by Repertoire Genesis Inc, RIKEN filed Critical Repertoire Genesis Inc

2019-05-22 Publication of EP3486321A1 publication Critical patent/EP3486321A1/en

2020-01-15 Publication of EP3486321A4 publication Critical patent/EP3486321A4/en

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Genetics & Genomics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Physics & Mathematics (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Chemical Kinetics & Catalysis (AREA)
Biomedical Technology (AREA)
Plant Pathology (AREA)
Bioinformatics & Computational Biology (AREA)
Crystallography & Structural Chemistry (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

EP17815521.4A 2016-06-23 2017-06-23 T-CELL RECEPTOR AND B-CELL RECEPTOR REPERTOIRE ANALYSIS SYSTEM USING A TEMPLATE-SWITCHING POLYMERASE CHAIN RACTION WITH ONE-STAGE REVERSE TRANSCRIPTION Withdrawn EP3486321A4 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP2016125007		2016-06-23
PCT/JP2017/023253 WO2017222056A1 (en)	2016-06-23	2017-06-23	T-cell receptor and b-cell receptor repertoire analysis system using one-step reverse transcription template-switching pcr

Publications (2)

Publication Number	Publication Date
EP3486321A1 EP3486321A1 (en)	2019-05-22
EP3486321A4 true EP3486321A4 (en)	2020-01-15

Family

ID=60783868

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
EP17815521.4A Withdrawn EP3486321A4 (en)	2016-06-23	2017-06-23	T-CELL RECEPTOR AND B-CELL RECEPTOR REPERTOIRE ANALYSIS SYSTEM USING A TEMPLATE-SWITCHING POLYMERASE CHAIN RACTION WITH ONE-STAGE REVERSE TRANSCRIPTION
EP17815522.2A Withdrawn EP3476944A4 (en)	2016-06-23	2017-06-23	TEMPLATE SWITCHING POLYMERASE CHAIN RACTION WITH ONE STAGE REVERSE TRANSCRIPTION

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
EP17815522.2A Withdrawn EP3476944A4 (en)	2016-06-23	2017-06-23	TEMPLATE SWITCHING POLYMERASE CHAIN RACTION WITH ONE STAGE REVERSE TRANSCRIPTION

Country Status (6)

Country	Link
US (2)	US20190169679A1 (en)
EP (2)	EP3486321A4 (en)
JP (2)	JP6924496B2 (en)
CN (2)	CN109328235A (en)
TW (1)	TWI758298B (en)
WO (2)	WO2017222057A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
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CA3032613A1 (en)	2016-11-10	2018-05-17	Takara Bio Usa, Inc.	Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
CA3096161A1 (en) *	2018-03-28	2019-10-03	Berkeley Lights, Inc.	Methods for preparation of nucleic acid sequencing libraries
CN112840033A (en) *	2018-08-22	2021-05-25	国立研究开发法人国立精神·神经医疗研究中心	Biomarkers of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)
WO2020040302A1 (en)	2018-08-24	2020-02-27	ＲｅｐｅｒｔｏｉｒｅＧｅｎｅｓｉｓ株式会社	Method for analyzing functional subunit pair gene of t cell receptor and b cell receptor
EP3670667A1 (en) *	2018-12-19	2020-06-24	Paris Sciences et Lettres - Quartier Latin	Identification of cognate pairs of ligands and receptors
JP2022544101A (en) *	2019-08-08	2022-10-17	アンスティチュナショナルドゥラサンテエドゥラルシェルシュメディカル	RNA-sequencing methods for the analysis of B and T cell transcriptomes in phenotypically defined B and T cell subsets
WO2021037368A1 (en) *	2019-08-29	2021-03-04	Institute For Research In Biomedicine	METHODS FOR RAPID cDNA PRODUCTION AND CLONING
CN113838528B (en) *	2021-09-02	2023-09-19	浙江大学	Single-cell level coupling visualization method based on single-cell immune repertoire data
AU2024231805A1 (en) *	2023-03-09	2025-09-18	Immunogeneteqs, Inc.	Method for amplifying complimentary dna strand

Citations (4)

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WO1997024455A2 (en) *	1996-01-03	1997-07-10	Clontech Laboratories, Inc.	METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING
WO2007139723A1 (en) *	2006-06-01	2007-12-06	Trilink Biotechnologies	Chemicallymodified oligonucleotide primers for nucleic acid amplification
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WO2017214417A1 (en) *	2016-06-10	2017-12-14	Takara Bio Usa, Inc.	Methods and compositions employing blocked primers

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US5773258A (en) *	1995-08-25	1998-06-30	Roche Molecular Systems, Inc.	Nucleic acid amplification using a reversibly inactivated thermostable enzyme
CA2725239C (en)	2008-05-27	2019-02-26	Trilink Biotechnologies	Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid
LT2756098T (en) *	2011-09-16	2018-09-10	Lexogen Gmbh	Method for making library of nucleic acid molecules
WO2014066179A1 (en) *	2012-10-24	2014-05-01	Clontech Laboratories, Inc.	Template switch-based methods for producing a product nucleic acid
US9382581B2 (en) *	2012-12-13	2016-07-05	Roche Molecular Systems, Inc.	Primers with modified phosphate and base in allele-specific PCR

2017
- 2017-06-23 EP EP17815521.4A patent/EP3486321A4/en not_active Withdrawn
- 2017-06-23 US US16/309,884 patent/US20190169679A1/en not_active Abandoned
- 2017-06-23 WO PCT/JP2017/023254 patent/WO2017222057A1/en not_active Ceased
- 2017-06-23 JP JP2018523697A patent/JP6924496B2/en active Active
- 2017-06-23 CN CN201780037392.0A patent/CN109328235A/en active Pending
- 2017-06-23 JP JP2018524187A patent/JP7066139B2/en active Active
- 2017-06-23 US US16/303,105 patent/US20190300934A1/en not_active Abandoned
- 2017-06-23 TW TW106121150A patent/TWI758298B/en not_active IP Right Cessation
- 2017-06-23 CN CN201780037451.4A patent/CN109312327A/en active Pending
- 2017-06-23 WO PCT/JP2017/023253 patent/WO2017222056A1/en not_active Ceased
- 2017-06-23 EP EP17815522.2A patent/EP3476944A4/en not_active Withdrawn

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WO1997024455A2 (en) *	1996-01-03	1997-07-10	Clontech Laboratories, Inc.	METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING
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WO2015075939A1 (en) *	2013-11-21	2015-05-28	ＲｅｐｅｒｔｏｉｒｅＧｅｎｅｓｉｓ株式会社	T cell receptor and b cell receptor repertoire analysis system, and use of same in treatment and diagnosis
US20160289760A1 (en) *	2013-11-21	2016-10-06	Repertoire Genesis Incorporation	T cell receptor and b cell receptor repertoire analysis system, and use of same in treatment and diagnosis
WO2017214417A1 (en) *	2016-06-10	2017-12-14	Takara Bio Usa, Inc.	Methods and compositions employing blocked primers

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Also Published As

Publication number	Publication date
EP3476944A4 (en)	2020-01-15
TWI758298B (en)	2022-03-21
TW201805432A (en)	2018-02-16
EP3486321A1 (en)	2019-05-22
CN109328235A (en)	2019-02-12
JPWO2017222056A1 (en)	2019-04-11
WO2017222056A1 (en)	2017-12-28
JP6924496B2 (en)	2021-08-25
EP3476944A1 (en)	2019-05-01
US20190169679A1 (en)	2019-06-06
JP7066139B2 (en)	2022-05-13
US20190300934A1 (en)	2019-10-03
CN109312327A (en)	2019-02-05
JPWO2017222057A1 (en)	2019-04-11
WO2017222057A1 (en)	2017-12-28

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2020-01-15	RIC1	Information provided on ipc code assigned before grant	Ipc: C12Q 1/6848 20180101ALI20191206BHEP Ipc: C12N 15/10 20060101ALI20191206BHEP Ipc: C12N 15/09 20060101ALI20191206BHEP Ipc: C12Q 1/68 20180101AFI20191206BHEP
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