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EP3455337A4 - Systèmes et procédés pour l'enrichissement d'un échantillon en cellules cibles - Google Patents

Systèmes et procédés pour l'enrichissement d'un échantillon en cellules cibles Download PDF

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Publication number
EP3455337A4
EP3455337A4 EP16901116.0A EP16901116A EP3455337A4 EP 3455337 A4 EP3455337 A4 EP 3455337A4 EP 16901116 A EP16901116 A EP 16901116A EP 3455337 A4 EP3455337 A4 EP 3455337A4
Authority
EP
European Patent Office
Prior art keywords
sample
systems
methods
target cells
enriching target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16901116.0A
Other languages
German (de)
English (en)
Other versions
EP3455337A1 (fr
Inventor
Ali Asgar S. Bhagat
Andrew En-Tzu WU
Junquan Huang
Guofeng Guan
Shuzhe CHAO
Yau Luong KOH
Yifang LEE
Man Chun LEONG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biolidics Ltd
Original Assignee
Biolidics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biolidics Ltd filed Critical Biolidics Ltd
Publication of EP3455337A1 publication Critical patent/EP3455337A1/fr
Publication of EP3455337A4 publication Critical patent/EP3455337A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56Labware specially adapted for transferring fluids
    • B01L3/567Valves, taps or stop-cocks
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0605Metering of fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0621Control of the sequence of chambers filled or emptied
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652Sorting or classification of particles or molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0684Venting, avoiding backpressure, avoid gas bubbles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/143Quality control, feedback systems
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088Channel loops
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883Serpentine channels
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0622Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083Concentrating samples by other techniques involving separation of suspended solids sedimentation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Fluid Mechanics (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
EP16901116.0A 2016-05-04 2016-05-04 Systèmes et procédés pour l'enrichissement d'un échantillon en cellules cibles Withdrawn EP3455337A4 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG2016/050205 WO2017192098A1 (fr) 2016-05-04 2016-05-04 Systèmes et procédés pour l'enrichissement d'un échantillon en cellules cibles

Publications (2)

Publication Number Publication Date
EP3455337A1 EP3455337A1 (fr) 2019-03-20
EP3455337A4 true EP3455337A4 (fr) 2020-01-01

Family

ID=60203155

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16901116.0A Withdrawn EP3455337A4 (fr) 2016-05-04 2016-05-04 Systèmes et procédés pour l'enrichissement d'un échantillon en cellules cibles

Country Status (7)

Country Link
US (1) US11059042B2 (fr)
EP (1) EP3455337A4 (fr)
JP (1) JP6924210B2 (fr)
CN (1) CN109072149B (fr)
AU (1) AU2016405636B2 (fr)
SG (1) SG11201809482TA (fr)
WO (1) WO2017192098A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3455337A4 (fr) * 2016-05-04 2020-01-01 Biolidics Limited Systèmes et procédés pour l'enrichissement d'un échantillon en cellules cibles
CN110951579A (zh) * 2019-08-09 2020-04-03 上海乘黄纳米抗体科技有限公司 免疫磁珠分选容器、分选装置及分选系统
US20220395831A1 (en) * 2019-10-21 2022-12-15 Nanyang Technological University Direct and scalable isolation of circulating extracellular vesicles from whole blood using centrifugal forces
US20210237064A1 (en) 2020-01-31 2021-08-05 Astrin Biosciences, Inc. Biological Fluid Filtration System
CN112903411B (zh) * 2021-01-25 2023-02-03 东南大学 一种多模式生物粒子浓缩仪
CN112958173B (zh) * 2021-03-04 2023-01-10 深圳市美好创亿医疗科技股份有限公司 一种微流控试剂盒
CN114806803A (zh) * 2022-04-18 2022-07-29 广州万孚生物技术股份有限公司 细胞分选系统及其分选方法
WO2024062903A1 (fr) * 2022-09-21 2024-03-28 キヤノン株式会社 Dispositif à microcanaux, dispositif de séparation et procédé de séparation

Citations (7)

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EP0901578A1 (fr) * 1996-05-31 1999-03-17 University of Washington Microdistributeur de liquide sans clapet
EP1593968A2 (fr) * 2004-05-06 2005-11-09 Seiko Instruments Inc. Dispositif, procédé et système d'analyse avec micropuces
US20110207621A1 (en) * 2008-02-21 2011-08-25 Avantra Biosciences Corporation Assays Based on Liquid Flow over Arrays
WO2015002975A1 (fr) * 2013-07-05 2015-01-08 University Of Washington Through Its Center For Commercialization Procédés, compositions et systèmes utilisables dans le cadre d'analyses microfluidiques
WO2015057159A1 (fr) * 2013-10-16 2015-04-23 Clearbridge Biomedics Pte Ltd Trieur microfluidique pour détection et isolation de cellules
US20150226657A1 (en) * 2014-02-13 2015-08-13 Owl biomedical, Inc. Microfluidic system with viscoelastic fluid
WO2016010861A1 (fr) * 2014-07-14 2016-01-21 President And Fellows Of Harvard College Systèmes et procédés pour une performance améliorée de systèmes fluidiques et microfluidiques

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JP5062658B2 (ja) 2006-07-28 2012-10-31 東京エレクトロン株式会社 導波管内の定在波測定部および定在波測定方法、電磁波利用装置、プラズマ処理装置およびプラズマ処理方法
JP2010151777A (ja) 2008-11-19 2010-07-08 Sony Corp 微小粒子解析装置、微小粒子解析用マイクロチップ及び微小粒子解析方法
ES2672366T3 (es) * 2009-03-17 2018-06-14 Menarini Silicon Biosystems S.P.A. Dispositivo microfluídico para el aislamiento de celdas
JP2010226978A (ja) * 2009-03-26 2010-10-14 Mitsui Eng & Shipbuild Co Ltd セルソータ
BRPI1106097A2 (pt) 2010-01-24 2017-06-27 Inst Chemy Fizycznej Polskiej Akademi Nauk sistema e método para produção e manipulação automazitada de misturas liquidas
US20140087412A1 (en) 2011-04-20 2014-03-27 4Dx Pty Ltd Method and Device for Application of Fluid Forces to Cells
JP2015524674A (ja) * 2012-08-17 2015-08-27 ユニバーシティ オブ セントラル フロリダ リサーチ ファウンデーション,インコーポレイテッド 哺乳動物系の機能的なインビトロ細胞モデルについての方法、システム、および組成物
CN105682802B (zh) * 2013-05-27 2018-03-16 星阵私人有限公司 一种微流控装置及控制其流体流动的方法
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WO2015200857A1 (fr) * 2014-06-27 2015-12-30 The Regents Of The University Of California Appareil et procédé pour analyse sans marqueur de cellules rares provenant de fluides corporels
CN104195028B (zh) * 2014-08-05 2017-07-25 深圳先进技术研究院 用于对特异性细胞进行筛选的微流控芯片及细胞筛选方法
EP3455337A4 (fr) * 2016-05-04 2020-01-01 Biolidics Limited Systèmes et procédés pour l'enrichissement d'un échantillon en cellules cibles

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0901578A1 (fr) * 1996-05-31 1999-03-17 University of Washington Microdistributeur de liquide sans clapet
EP0901578B1 (fr) * 1996-05-31 2002-01-02 University of Washington Microdistributeur de liquide sans clapet e methode
EP1593968A2 (fr) * 2004-05-06 2005-11-09 Seiko Instruments Inc. Dispositif, procédé et système d'analyse avec micropuces
US20110207621A1 (en) * 2008-02-21 2011-08-25 Avantra Biosciences Corporation Assays Based on Liquid Flow over Arrays
WO2015002975A1 (fr) * 2013-07-05 2015-01-08 University Of Washington Through Its Center For Commercialization Procédés, compositions et systèmes utilisables dans le cadre d'analyses microfluidiques
WO2015057159A1 (fr) * 2013-10-16 2015-04-23 Clearbridge Biomedics Pte Ltd Trieur microfluidique pour détection et isolation de cellules
US20150226657A1 (en) * 2014-02-13 2015-08-13 Owl biomedical, Inc. Microfluidic system with viscoelastic fluid
WO2016010861A1 (fr) * 2014-07-14 2016-01-21 President And Fellows Of Harvard College Systèmes et procédés pour une performance améliorée de systèmes fluidiques et microfluidiques

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Title
See also references of WO2017192098A1 *

Also Published As

Publication number Publication date
US11059042B2 (en) 2021-07-13
CN109072149B (zh) 2022-02-18
JP6924210B2 (ja) 2021-08-25
AU2016405636B2 (en) 2019-12-05
US20190151847A1 (en) 2019-05-23
AU2016405636A1 (en) 2018-11-15
CN109072149A (zh) 2018-12-21
JP2019515678A (ja) 2019-06-13
EP3455337A1 (fr) 2019-03-20
SG11201809482TA (en) 2018-11-29
WO2017192098A1 (fr) 2017-11-09

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