EP3370744A4 - Le blocage du cycle cellulaire améliore l'efficacité de production de cellules souches pluripotentes induites - Google Patents

Le blocage du cycle cellulaire améliore l'efficacité de production de cellules souches pluripotentes induites Download PDF

Info

Publication number: EP3370744A4
Authority: EP; European Patent Office
Prior art keywords: blocking; efficiency; pluripotent stem; induced pluripotent; stem cell
Prior art date: 2015-11-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP16862739.6A

Other languages

German (de)

English (en)

Other versions

EP3370744A1 (fr

Inventor

Robin Y. SMITH

Marcie A. Glicksman

Nikhat F. ZAIDI

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Seaport Diagnostics Inc

Original Assignee

Orig3n Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2015-11-02

Filing date

2016-10-27

Publication date

2019-04-17

2016-10-27 Application filed by Orig3n Inc filed Critical Orig3n Inc

2018-09-12 Publication of EP3370744A1 publication Critical patent/EP3370744A1/fr

2019-04-17 Publication of EP3370744A4 publication Critical patent/EP3370744A4/fr

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
- A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Cell Biology (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Developmental Biology & Embryology (AREA)
Chemical & Material Sciences (AREA)
Biotechnology (AREA)
Zoology (AREA)
Immunology (AREA)
General Health & Medical Sciences (AREA)
Epidemiology (AREA)
Pharmacology & Pharmacy (AREA)
Virology (AREA)
Animal Behavior & Ethology (AREA)
Medicinal Chemistry (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Wood Science & Technology (AREA)
Reproductive Health (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Hematology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Gynecology & Obstetrics (AREA)
Transplantation (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Materials For Medical Uses (AREA)

EP16862739.6A 2015-11-02 2016-10-27 Le blocage du cycle cellulaire améliore l'efficacité de production de cellules souches pluripotentes induites Withdrawn EP3370744A4 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US201562249520P	2015-11-02	2015-11-02
PCT/US2016/059112 WO2017079029A1 (fr)	2015-11-02	2016-10-27	Le blocage du cycle cellulaire améliore l'efficacité de production de cellules souches pluripotentes induites

Publications (2)

Publication Number	Publication Date
EP3370744A1 EP3370744A1 (fr)	2018-09-12
EP3370744A4 true EP3370744A4 (fr)	2019-04-17

Family

ID=58662634

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP16862739.6A Withdrawn EP3370744A4 (fr)	2015-11-02	2016-10-27	Le blocage du cycle cellulaire améliore l'efficacité de production de cellules souches pluripotentes induites

Country Status (6)

Country	Link
EP (1)	EP3370744A4 (fr)
JP (1)	JP2018531610A (fr)
KR (1)	KR20180072817A (fr)
CN (1)	CN108348556A (fr)
IL (1)	IL258640A (fr)
WO (1)	WO2017079029A1 (fr)

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* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN107589002B (zh) *	2017-09-08	2020-05-29	杭州市妇产科医院	一种诱导多能干细胞染色体的制备方法
JP7336386B2 (ja) *	2017-12-28	2023-08-31	株式会社カネカ	多能性幹細胞凝集抑制剤
CN110016460A (zh) *	2018-01-09	2019-07-16	中国农业大学	一种高效鸡胚胎原代成纤维细胞g2/m期同步化方法
CN108531447B (zh) *	2018-04-13	2021-11-23	上海市生物医药技术研究院	调节精子运动能力及辅助生殖的化合物及其用途
CN115531382B (zh) *	2021-06-29	2024-01-30	中国科学院生物物理研究所	喹啉噻唑啉酮衍生物Ro-3306在治疗β-冠状病毒感染中的应用
CN113462638B (zh) *	2021-06-30	2022-10-25	呈诺再生医学科技(珠海横琴新区)有限公司	一种高效无遗传修饰的iPSC诱导、产业化单克隆挑取平台及应用
JPWO2023243679A1 (fr) *	2022-06-17	2023-12-21
CN119685438B (zh) *	2025-02-24	2025-07-29	北京大学口腔医学院	用于评价纳米颗粒对细胞毒性的方法及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2010111422A2 (fr) *	2009-03-25	2010-09-30	The Salk Institute For Biological Studies	Génération de cellules souches pluripotentes induites en utilisant deux facteurs et l'inactivation de p53
WO2011060100A1 (fr) *	2009-11-11	2011-05-19	Sanford-Burnham Medical Research Institute	Procédé de production et de régulation de cellules ips et compositions associées
WO2012136841A1 (fr) *	2011-04-08	2012-10-11	INSERM (Institut National de la Santé et de la Recherche Médicale)	Procédé pour régénérer des cellules

Family Cites Families (6)

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Publication number	Priority date	Publication date	Assignee	Title
US6033847A (en) *	1995-02-06	2000-03-07	St. Jude Children's Research Hospital	InK4c-p18 and InK4d-p19, inhibitors of cyclin-dependent kinases CDK4 and CDK6, and uses thereof
JP2001509017A (ja) *	1997-01-23	2001-07-10	ザ・ジョーンズ・ホプキンス・ユニバーシティ・スクール・オブ・メディシン	イーストトランスクリプトームのキャラクタリゼーション
JP2008307007A (ja) *	2007-06-15	2008-12-25	Bayer Schering Pharma Ag	出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
WO2010004989A1 (fr) *	2008-07-07	2010-01-14	タカラバイオ株式会社	Méthode de production d’une cellule souche pluripotente
CN104278008B (zh) *	2013-07-12	2020-08-21	北京宏冠再生医学科技有限公司	一种通过小分子化合物处理来制备多潜能干细胞的方法、试剂盒和用途
JP6232907B2 (ja) *	2013-10-09	2017-11-22	富士レビオ株式会社	融合細胞およびその作製方法

2016
- 2016-10-27 EP EP16862739.6A patent/EP3370744A4/fr not_active Withdrawn
- 2016-10-27 CN CN201680063137.9A patent/CN108348556A/zh active Pending
- 2016-10-27 KR KR1020187015171A patent/KR20180072817A/ko not_active Withdrawn
- 2016-10-27 JP JP2018521503A patent/JP2018531610A/ja active Pending
- 2016-10-27 WO PCT/US2016/059112 patent/WO2017079029A1/fr not_active Ceased
2018
- 2018-04-11 IL IL258640A patent/IL258640A/en unknown

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2010111422A2 (fr) *	2009-03-25	2010-09-30	The Salk Institute For Biological Studies	Génération de cellules souches pluripotentes induites en utilisant deux facteurs et l'inactivation de p53
WO2011060100A1 (fr) *	2009-11-11	2011-05-19	Sanford-Burnham Medical Research Institute	Procédé de production et de régulation de cellules ips et compositions associées
WO2012136841A1 (fr) *	2011-04-08	2012-10-11	INSERM (Institut National de la Santé et de la Recherche Médicale)	Procédé pour régénérer des cellules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KESHI CHEN ET AL: "Gadd45a is a heterochromatin relaxer that enhances iPS cell generation", EMBO REPORTS, vol. 17, no. 11, 4 October 2015 (2015-10-04), pages 1641 - 1656, XP055564950, ISSN: 1469-3178, DOI: 10.15252/embr.201642402 *
See also references of WO2017079029A1 *

Also Published As

Publication number	Publication date
EP3370744A1 (fr)	2018-09-12
KR20180072817A (ko)	2018-06-29
IL258640A (en)	2018-06-28
JP2018531610A (ja)	2018-11-01
WO2017079029A1 (fr)	2017-05-11
CN108348556A (zh)	2018-07-31

Publication	Publication Date	Title
EP3370744A4 (fr)	2019-04-17	Le blocage du cycle cellulaire améliore l'efficacité de production de cellules souches pluripotentes induites
EP3334820C0 (fr)	2024-05-15	Cellules souches pluripotentes étendues et induites, procédés de fabrication et d'utilisation
IL259586B (en)	2022-10-01	Genetically modified stem cells and uses thereof
EP3510145A4 (fr)	2020-03-25	Cellules immunitaires dérivées de cellules souches pluripotentes induites
PL3288719T3 (pl)	2022-07-04	Zestaw użytkowy
DK3702443T3 (da)	2022-04-04	Sammensætninger og fremgangsmåder til opformering og dyrkning af epiteliale stamceller
IL250689A0 (en)	2017-04-30	Resetting pluripotent stem cells
EP3351615A4 (fr)	2019-05-15	Récipient de culture cellulaire
EP3350313A4 (fr)	2019-06-12	Dérivation d'organoïdes hépatiques à partir de cellules souches pluripotentes humaines
EP3221446C0 (fr)	2024-08-21	Production in vitro de cellules souches potentielles expansées
IL267308A (en)	2019-08-29	Plants with a shortened flowering time
EP3315596A4 (fr)	2019-02-13	Récipient de culture cellulaire
GB201709599D0 (en)	2017-08-02	Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
IL263265A (en)	2018-12-31	Use of mesenchymal stem cells and their parts
IL258829A (en)	2018-06-28	A process to enhance the bioactivity of ashwagandha extracts
EP3455344A4 (fr)	2019-12-18	Cellules souches/progénitrices hématopoïétiques
DK3463538T3 (da)	2024-10-21	Oxygengaskoncentrator med udløbsakkumulator
EP3374490A4 (fr)	2019-08-28	Expression hétérologue de la taurine dans des micro-organismes
EP3541924A4 (fr)	2019-12-18	Dosage de cellules souches pluripotentes
DK3586821T3 (da)	2022-01-17	Stamcellestimulerende sammensætninger og metoder
IL255500B (en)	2021-04-29	Methods for assesing the purity of a mesenchymal stem cells preparation
EP3448986A4 (fr)	2020-01-15	Culture de cellules
AU364088S (en)	2015-09-11	Upper frame
DK3662058T3 (da)	2024-07-29	Generering af oligodendrogene neurale stamceller
IL270880A (en)	2020-01-30	Cell culture methods

Legal Events

Date	Code	Title	Description
2017-05-13	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE
2018-08-10	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2018-08-10	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE
2018-09-12	17P	Request for examination filed	Effective date: 20180502
2018-09-12	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
2018-09-12	AX	Request for extension of the european patent	Extension state: BA ME
2019-02-06	DAV	Request for validation of the european patent (deleted)
2019-02-06	DAX	Request for extension of the european patent (deleted)
2019-04-17	A4	Supplementary search report drawn up and despatched	Effective date: 20190319
2019-04-17	RIC1	Information provided on ipc code assigned before grant	Ipc: C12N 5/071 20100101ALI20190313BHEP Ipc: A61K 35/17 20150101ALI20190313BHEP Ipc: A61K 35/545 20150101AFI20190313BHEP Ipc: A61K 35/12 20150101ALI20190313BHEP Ipc: A61K 35/35 20150101ALI20190313BHEP
2021-06-18	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: EXAMINATION IS IN PROGRESS
2021-07-21	17Q	First examination report despatched	Effective date: 20210621
2022-04-29	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2022-06-01	18D	Application deemed to be withdrawn	Effective date: 20220104