EP3287523A4 - Procédé de production d'une protéine par sécrétion - Google Patents

Procédé de production d'une protéine par sécrétion Download PDF

Info

Publication number: EP3287523A4
Authority: EP; European Patent Office
Prior art keywords: protein; secretory production; secretory; production
Prior art date: 2015-04-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP16783245.0A

Other languages

German (de)

English (en)

Other versions

EP3287523B1 (fr

EP3287523A1 (fr

Inventor

Yoshihiko Matsuda

Yumi Ito

Yukari KASHIMA

Naoko Yamada

Noriko TSURUI

Hiroshi Itaya

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ajinomoto Co Inc

Original Assignee

Ajinomoto Co Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2015-04-24

Filing date

2016-04-21

Publication date

2018-09-05

2016-04-21 Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc

2018-02-28 Publication of EP3287523A1 publication Critical patent/EP3287523A1/fr

2018-09-05 Publication of EP3287523A4 publication Critical patent/EP3287523A4/fr

2022-06-29 Application granted granted Critical

2022-06-29 Publication of EP3287523B1 publication Critical patent/EP3287523B1/fr

Status Active legal-status Critical Current

2036-04-21 Anticipated expiration legal-status Critical

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/13—Protein-histidine kinases (2.7.13)
- C12Y207/13003—Histidine kinase (2.7.13.3)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Peptides Or Proteins (AREA)

EP16783245.0A 2015-04-24 2016-04-21 Procédé de production d'une protéine par sécrétion Active EP3287523B1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP2015089046		2015-04-24
PCT/JP2016/062675 WO2016171224A1 (fr)	2015-04-24	2016-04-21	Procédé de production d'une protéine par sécrétion

Publications (3)

Publication Number	Publication Date
EP3287523A1 EP3287523A1 (fr)	2018-02-28
EP3287523A4 true EP3287523A4 (fr)	2018-09-05
EP3287523B1 EP3287523B1 (fr)	2022-06-29

Family

ID=57143158

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP16783245.0A Active EP3287523B1 (fr)	2015-04-24	2016-04-21	Procédé de production d'une protéine par sécrétion

Country Status (8)

Country	Link
US (1)	US10538798B2 (fr)
EP (1)	EP3287523B1 (fr)
JP (1)	JP6741000B2 (fr)
KR (1)	KR102619848B1 (fr)
CN (1)	CN107532163A (fr)
AU (1)	AU2016253217B2 (fr)
DK (1)	DK3287523T3 (fr)
WO (1)	WO2016171224A1 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2018074579A1 (fr)	2016-10-21	2018-04-26	味の素株式会社	Procédé de production sécrétoire de protéines
WO2018074578A1 (fr)	2016-10-21	2018-04-26	味の素株式会社	Procédé de production sécrétoire de protéine
WO2019078095A1 (fr)	2017-10-16	2019-04-25	Ajinomoto Co., Inc.	Procédé de production d'une protéine ayant une activité peptidylglycine monooxygénase alpha-hydroxylante
CN108330095B (zh) *	2018-03-01	2020-12-29	江南大学	一种积累n-乙酰神经氨酸的重组谷氨酸棒杆菌及其应用
AU2019256065B2 (en) *	2018-04-20	2024-11-28	Ajinomoto Co., Inc.	Method for secretory production of protein
CN110511953B (zh) *	2018-08-31	2022-09-30	江南大学	一种适用于谷氨酸棒杆菌的重组表达载体、外源蛋白表达系统、应用和木聚糖酶的制备方法
EP3861109A1 (fr)	2018-10-05	2021-08-11	Ajinomoto Co., Inc.	Procédé de production d'une substance cible par fermentation bactérienne
AU2019364829B2 (en) *	2018-10-25	2025-11-20	Ajinomoto Co., Inc.	Method for secretory production of protein
CN113227124B (zh)	2018-10-31	2025-04-08	味之素株式会社	具有针对抗体的亲和性物质、切割性部分及反应性基团的化合物或其盐
CN111434770B (zh) *	2019-01-11	2022-12-06	华东师范大学	蛋白质谷氨酰胺酶酶原在枯草芽孢杆菌中的表达和自加工
AU2020396647A1 (en)	2019-12-06	2022-07-07	Ajinomoto Co., Inc.	Method for producing peptide having physiological activity, and peptide comprising short linker
JPWO2023282315A1 (fr)	2021-07-07	2023-01-12
EP4417684A1 (fr) *	2021-10-11	2024-08-21	Ajinomoto Co., Inc.	Bactérie modifiée pour exprimer une protéine tat hétérologue
JPWO2023200008A1 (fr)	2022-04-15	2023-10-19
EP4534547A1 (fr)	2022-06-02	2025-04-09	Ajinomoto Co., Inc.	Substance d'affinité, composé, anticorps et leurs sels
CN115806985B (zh) *	2022-10-25	2025-09-05	广东肇庆星湖生物科技股份有限公司	适用于停滞棒杆菌的启动子及应用
JPWO2024172163A1 (fr)	2023-02-16	2024-08-22

Citations (3)

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Publication number	Priority date	Publication date	Assignee	Title
WO2004035792A1 (fr) *	2002-10-16	2004-04-29	Celltech R & D Limited	Cellules hotes e. coli comprenant des proteines a liaison phosphate periplasmiques phos/psts modifiees et procede de production de fabs recombinants
WO2006069610A2 (fr) *	2004-07-02	2006-07-06	Metanomics Gmbh	Procede de production de produits chimiques fins
EP1748077A1 (fr) *	2004-04-20	2007-01-31	Ajinomoto Co., Inc.	Procédé consistant à produire une protéine

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JPS592689A (ja) *	1982-06-04	1984-01-09	Handai Biseibutsubiyou Kenkyukai	強力な遺伝子発現能を有する新規レプリコンの作成法
US4965197A (en)	1987-06-12	1990-10-23	Massachusetts Institute Of Technology	Coryneform expression and secretion system
JPH06502548A (ja)	1991-07-30	1994-03-24	オルサン	特にコリネバクテリア中で用いることのできる蛋白質の発現および分泌系
JPH11169182A (ja)	1997-12-10	1999-06-29	Mitsubishi Chemical Corp	コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
CN100497632C (zh)	1999-09-30	2009-06-10	味之素株式会社	转谷氨酰胺酶的制备方法
CN1250722C (zh)	2001-03-30	2006-04-12	味之素株式会社	分泌生产蛋白质的方法
CN100554431C (zh) *	2004-04-20	2009-10-28	味之素株式会社	生产蛋白质的方法
CN101675069A (zh) *	2004-07-02	2010-03-17	梅坦诺米克斯有限公司	产生精细化学品的方法
US7618799B2 (en) *	2007-01-31	2009-11-17	Dow Global Technologies Inc	Bacterial leader sequences for increased expression
AU2012329967B2 (en) *	2011-10-25	2015-07-23	Ajinomoto Co., Inc.	Secretion production method for protein
JP6136930B2 (ja)	2011-11-02	2017-05-31	味の素株式会社	タンパク質の分泌生産法
KR101653555B1 (ko)	2011-11-02	2016-09-02	아지노모토 가부시키가이샤	단백질의 분비 생산 방법
JPWO2013118544A1 (ja) *	2012-02-08	2015-05-11	味の素株式会社	タンパク質の分泌生産法
JP6309317B2 (ja)	2013-03-28	2018-04-11	株式会社半導体エネルギー研究所	発光素子及び化合物

2016
- 2016-04-21 AU AU2016253217A patent/AU2016253217B2/en active Active
- 2016-04-21 KR KR1020177034151A patent/KR102619848B1/ko active Active
- 2016-04-21 WO PCT/JP2016/062675 patent/WO2016171224A1/fr not_active Ceased
- 2016-04-21 DK DK16783245.0T patent/DK3287523T3/da active
- 2016-04-21 JP JP2017514190A patent/JP6741000B2/ja active Active
- 2016-04-21 CN CN201680023752.7A patent/CN107532163A/zh active Pending
- 2016-04-21 EP EP16783245.0A patent/EP3287523B1/fr active Active
2017
- 2017-10-23 US US15/790,495 patent/US10538798B2/en active Active

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2004035792A1 (fr) *	2002-10-16	2004-04-29	Celltech R & D Limited	Cellules hotes e. coli comprenant des proteines a liaison phosphate periplasmiques phos/psts modifiees et procede de production de fabs recombinants
EP1748077A1 (fr) *	2004-04-20	2007-01-31	Ajinomoto Co., Inc.	Procédé consistant à produire une protéine
WO2006069610A2 (fr) *	2004-07-02	2006-07-06	Metanomics Gmbh	Procede de production de produits chimiques fins

Non-Patent Citations (2)

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Title
MICHAEL BOTT ET AL: "Two-component signal transduction inand other corynebacteria: on the way towards stimuli and targets", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 94, no. 5, 28 April 2012 (2012-04-28), pages 1131 - 1150, XP035056736, ISSN: 1432-0614, DOI: 10.1007/S00253-012-4060-X *
See also references of WO2016171224A1 *

Also Published As

Publication number	Publication date
WO2016171224A9 (fr)	2017-09-28
US10538798B2 (en)	2020-01-21
EP3287523B1 (fr)	2022-06-29
CN107532163A (zh)	2018-01-02
KR20170140351A (ko)	2017-12-20
WO2016171224A1 (fr)	2016-10-27
KR102619848B1 (ko)	2024-01-04
JP6741000B2 (ja)	2020-08-19
US20180037918A1 (en)	2018-02-08
JPWO2016171224A1 (ja)	2018-02-22
DK3287523T3 (da)	2022-07-25
EP3287523A1 (fr)	2018-02-28
AU2016253217A1 (en)	2017-11-23
AU2016253217B2 (en)	2021-09-02

Publication	Publication Date	Title
EP3287523A4 (fr)	2018-09-05	Procédé de production d'une protéine par sécrétion
EP3530747A4 (fr)	2020-07-22	Procédé de production sécrétoire de protéines
EP3297661A4 (fr)	2018-11-21	Procédé d'amélioration des caractéristiques de protéines
EP3147369A4 (fr)	2018-02-07	Procédé de production de protéines de type fibroïne
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EP3434782A4 (fr)	2019-03-20	Procédé de production de protéines
EP3196304A4 (fr)	2018-01-10	Procédé destiné à la production sécrétoire élevée améliorée de protéines
EP3783106A4 (fr)	2022-01-05	Procédé de production par sécrétion d'une protéine
EP3272789A4 (fr)	2018-01-24	Procédé de fabrication d'un mélange
EP3147368A4 (fr)	2018-01-17	Procédé de production de protéine de type fibroïne
IL252712A0 (en)	2017-08-31	Method for protein production
EP3281938A4 (fr)	2018-02-14	Procédé de production d'acide bisimidodicarboxylique
EP3386944A4 (fr)	2019-08-28	Procédé de préparation de lisdexamphétamine
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EP3131567A4 (fr)	2017-11-15	Procédé de production de gel contenant de la gnrh
EP3207020A4 (fr)	2018-03-07	Procédé de préparation de la vitamine k1
EP3252163A4 (fr)	2018-09-26	Procédé de production de substance
EP3315585A4 (fr)	2018-05-30	Procédé de production de ferro-coke

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2018-09-05	RIC1	Information provided on ipc code assigned before grant	Ipc: C12P 21/00 20060101ALI20180802BHEP Ipc: C07K 14/34 20060101ALI20180802BHEP Ipc: C12N 1/21 20060101ALI20180802BHEP Ipc: C12N 15/09 20060101AFI20180802BHEP
2018-10-17	RIC1	Information provided on ipc code assigned before grant	Ipc: C12N 1/21 20060101ALI20180907BHEP Ipc: C07K 14/34 20060101ALI20180907BHEP Ipc: C12N 15/09 20060101AFI20180907BHEP Ipc: C12P 21/00 20060101ALI20180907BHEP
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