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EP3286338C0 - AMPLIFICATION WITH PRIMERS OF A LIMITED NUCLEOTIDE COMPOSITION - Google Patents

AMPLIFICATION WITH PRIMERS OF A LIMITED NUCLEOTIDE COMPOSITION

Info

Publication number
EP3286338C0
EP3286338C0 EP16784032.1A EP16784032A EP3286338C0 EP 3286338 C0 EP3286338 C0 EP 3286338C0 EP 16784032 A EP16784032 A EP 16784032A EP 3286338 C0 EP3286338 C0 EP 3286338C0
Authority
EP
European Patent Office
Prior art keywords
primers
amplification
nucleotide composition
limited nucleotide
limited
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP16784032.1A
Other languages
German (de)
French (fr)
Other versions
EP3286338A2 (en
EP3286338B1 (en
EP3286338A4 (en
Inventor
Youxiang Wang
Zhijie Yang
Xin Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Atila BioSystems Inc
Original Assignee
Atila BioSystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atila BioSystems Inc filed Critical Atila BioSystems Inc
Publication of EP3286338A2 publication Critical patent/EP3286338A2/en
Publication of EP3286338A4 publication Critical patent/EP3286338A4/en
Application granted granted Critical
Publication of EP3286338B1 publication Critical patent/EP3286338B1/en
Publication of EP3286338C0 publication Critical patent/EP3286338C0/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/143—Modifications characterised by incorporating a promoter sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP16784032.1A 2015-04-24 2016-04-22 Amplification with primers of limited nucleotide composition Active EP3286338B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562152756P 2015-04-24 2015-04-24
PCT/US2016/029054 WO2016172632A2 (en) 2015-04-24 2016-04-22 Amplification with primers of limited nucleotide composition

Publications (4)

Publication Number Publication Date
EP3286338A2 EP3286338A2 (en) 2018-02-28
EP3286338A4 EP3286338A4 (en) 2018-12-19
EP3286338B1 EP3286338B1 (en) 2023-11-01
EP3286338C0 true EP3286338C0 (en) 2023-11-01

Family

ID=57143585

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16784032.1A Active EP3286338B1 (en) 2015-04-24 2016-04-22 Amplification with primers of limited nucleotide composition

Country Status (8)

Country Link
US (2) US11091799B2 (en)
EP (1) EP3286338B1 (en)
JP (2) JP6966681B2 (en)
CN (2) CN114250274A (en)
AU (2) AU2016252998B2 (en)
CA (1) CA2983819A1 (en)
ES (1) ES2961374T3 (en)
WO (1) WO2016172632A2 (en)

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CN114250274A (en) 2015-04-24 2022-03-29 阿提拉生物系统公司 Amplification of primers with limited nucleotide composition
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CA3058202A1 (en) * 2017-04-17 2018-10-25 Montana State University Switch-like isothermal dna amplification demonstrating a non-linear amplification rate
AU2018313286B2 (en) * 2017-08-11 2024-09-12 Atila Biosystems Incorporated Digital amplification with primers of limited nucleotide composition
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
EP3743525A4 (en) 2018-01-26 2021-09-29 President and Fellows of Harvard College PROXIMITY DETECTION METHODS AND COMPOSITIONS
KR102180462B1 (en) * 2018-01-31 2020-11-18 한국과학기술원 Method for Detecting Target Nucleic Acid using Three-way Junction Structure-induced Isothermal Amplification〔ThIsAmp〕
JP7013960B2 (en) * 2018-03-13 2022-02-01 株式会社リコー Nucleic acid detection method and nucleic acid detection device, and nucleic acid detection kit
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CN112899357A (en) * 2021-01-05 2021-06-04 南京普济生物有限公司 Method for detecting fetal fraction by using digital PCR and kit thereof
CN112899346A (en) * 2021-01-05 2021-06-04 南京普济生物有限公司 Nucleotide for reducing PCR (polymerase chain reaction) nonspecific amplification and design method and application thereof
CN112899366A (en) * 2021-01-06 2021-06-04 南京普济生物有限公司 Method and kit for detecting colorectal cancer gene mutation site
CN112735527B (en) * 2021-01-06 2022-09-13 武汉华大基因技术服务有限公司 Method, device and storage medium for analyzing series sequence
CN112899365A (en) * 2021-01-06 2021-06-04 南京普济生物有限公司 Detection method and kit for lung cancer gene mutation site
CN112899358A (en) * 2021-01-06 2021-06-04 南京普济生物有限公司 Noninvasive prenatal fetal chromosome aneuploidy detection method and kit thereof
CN114958988A (en) * 2021-02-27 2022-08-30 上海境象生物科技有限公司 Multiple nucleic acid detection method and kit based on probe melting curve analysis
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Also Published As

Publication number Publication date
AU2016252998A1 (en) 2017-12-14
ES2961374T3 (en) 2024-03-11
EP3286338A2 (en) 2018-02-28
US11091799B2 (en) 2021-08-17
US20180148775A1 (en) 2018-05-31
JP6966681B2 (en) 2021-11-17
EP3286338B1 (en) 2023-11-01
CN107849603B (en) 2022-01-28
US20220033891A1 (en) 2022-02-03
CN114250274A (en) 2022-03-29
AU2016252998B2 (en) 2021-11-04
JP2022008577A (en) 2022-01-13
JP2018514230A (en) 2018-06-07
EP3286338A4 (en) 2018-12-19
CN107849603A (en) 2018-03-27
CA2983819A1 (en) 2016-10-27
WO2016172632A2 (en) 2016-10-27
WO2016172632A3 (en) 2017-01-12
AU2022200725A1 (en) 2022-02-24

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