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EP3063269A4 - Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells - Google Patents

Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells Download PDF

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Publication number
EP3063269A4
EP3063269A4 EP14859059.9A EP14859059A EP3063269A4 EP 3063269 A4 EP3063269 A4 EP 3063269A4 EP 14859059 A EP14859059 A EP 14859059A EP 3063269 A4 EP3063269 A4 EP 3063269A4
Authority
EP
European Patent Office
Prior art keywords
clustering
differentiation
suspension
pluripotent stem
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14859059.9A
Other languages
German (de)
French (fr)
Other versions
EP3063269A1 (en
Inventor
Benjamin Fryer
Daina LANIAUSKAS
Marcia BLACKMOORE
Haiyun Wang
Kostadinka LILOVA
Shelley Nelson
Elizabeth ROSOCHA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Biotech Inc
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of EP3063269A1 publication Critical patent/EP3063269A1/en
Publication of EP3063269A4 publication Critical patent/EP3063269A4/en
Withdrawn legal-status Critical Current

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    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0676Pancreatic cells
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    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
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EP14859059.9A 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells Withdrawn EP3063269A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/042796 WO2015065537A1 (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Publications (2)

Publication Number Publication Date
EP3063269A1 EP3063269A1 (en) 2016-09-07
EP3063269A4 true EP3063269A4 (en) 2017-07-12

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Family Applications (2)

Application Number Title Priority Date Filing Date
EP14857034.4A Withdrawn EP3063268A4 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
EP14859059.9A Withdrawn EP3063269A4 (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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Application Number Title Priority Date Filing Date
EP14857034.4A Withdrawn EP3063268A4 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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EP (2) EP3063268A4 (en)
JP (2) JP2016534731A (en)
KR (4) KR20160079072A (en)
CN (2) CN105793413A (en)
AU (4) AU2014342995C1 (en)
BR (1) BR112016009393A8 (en)
CA (2) CA2928639A1 (en)
MX (2) MX2016005657A (en)
PH (2) PH12016500783A1 (en)
RU (2) RU2689710C2 (en)
SG (2) SG11201603045VA (en)
WO (2) WO2015065524A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2016005657A (en) * 2013-11-01 2017-01-05 Janssen Biotech Inc Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells.
WO2017223233A1 (en) * 2016-06-23 2017-12-28 Tithon Biotech, Inc. Cells expressing parathyroid hormone 1 receptor and uses thereof
JP7088564B2 (en) 2016-11-10 2022-06-21 ヴィアサイト,インコーポレイテッド PDX1 pancreatic endoderm cells and methods thereof in a cell delivery device
CA3042562C (en) * 2016-11-16 2023-02-28 Cynata Therapeutics Limited Pluripotent stem cell assay
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
EP4488363A3 (en) * 2017-07-21 2025-03-26 Vertex Pharmaceuticals Incorporated Re-aggregation of stem cell-derived pancreatic beta cells
JP7000896B2 (en) * 2017-08-23 2022-01-19 王子ホールディングス株式会社 Cell sheet forming member, method for manufacturing cell sheet forming member, and method for manufacturing cell sheet
MX2020007071A (en) * 2018-01-05 2020-11-11 Platelet Biogenesis Inc Compositions and methods for producing megakaryocytes.
US12104173B2 (en) 2018-01-18 2024-10-01 Agency For Science, Technology And Research Method for differentiation of human pluripotent stem cell lines in suspension culture
KR102115360B1 (en) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 Adult stem cell culture fluid and a producing method thereof
US10724052B2 (en) 2018-09-07 2020-07-28 Crispr Therapeutics Ag Universal donor cells
EP3975926A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates, Inc. A biocompatible membrane composite
EP3976237A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. Cell encapsulation devices with controlled oxygen diffusion distances
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
CA3139591C (en) 2019-05-31 2024-01-16 Timothy M. BRUHN A biocompatible membrane composite
JP2022547417A (en) * 2019-08-27 2022-11-14 ユナイテッド セラピューティクス コーポレイション Methods and compositions for culturing alveolar cells
US11118195B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
AU2020340622A1 (en) 2019-09-05 2022-03-03 Crispr Therapeutics Ag Universal donor cells
EP4061929A1 (en) * 2019-11-22 2022-09-28 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
AU2021414405A1 (en) 2020-12-31 2023-08-10 Crispr Therapeutics Ag Universal donor cells
WO2022203051A1 (en) * 2021-03-25 2022-09-29 株式会社カネカ Method for producing pluripotent stem cell population
CN116218766A (en) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 A method for preparing pancreatic precursor cells, culture medium and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014106141A1 (en) * 2012-12-31 2014-07-03 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
WO2015065524A2 (en) * 2013-11-01 2015-05-07 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005533480A (en) * 2001-11-09 2005-11-10 アーテセル・サイエンシズ・インコーポレーテツド Pancreatic endocrine differentiation of adipose tissue-derived stromal cells and use thereof
DK3441459T3 (en) * 2006-08-02 2021-06-07 Technion Res & Dev Foundation PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE
EP2088190A4 (en) * 2006-11-09 2011-01-05 Japan Government METHOD FOR THE CULTURE AND PASSAGE OF PRIMATE EMBRYONIC STRAIN CELL, AND METHOD FOR INDUCING DIFFERENTIATION OF EMBRYONIC STEM CELL
US20090053182A1 (en) * 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
WO2009006399A1 (en) * 2007-07-01 2009-01-08 Lifescan Single pluripotent stem cell culture
JP2011514169A (en) * 2008-03-17 2011-05-06 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ Microcarrier for stem cell culture
AU2009267137A1 (en) * 2008-06-30 2010-01-07 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
CA2770753C (en) * 2009-08-12 2019-01-15 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
JP5936134B2 (en) * 2010-06-15 2016-06-15 国立大学法人京都大学 Selection method of human induced pluripotent stem cells
KR101836850B1 (en) * 2010-08-31 2018-03-09 얀센 바이오테크 인코포레이티드 Differentiation of human embryonic stem cells
MY177150A (en) * 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013056072A1 (en) * 2011-10-13 2013-04-18 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
AU2012355698B2 (en) * 2011-12-22 2018-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014106141A1 (en) * 2012-12-31 2014-07-03 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
WO2015065524A2 (en) * 2013-11-01 2015-05-07 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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