EP2796208A1 - Procédé pour commander une cellule acoustique - Google Patents
Procédé pour commander une cellule acoustique Download PDFInfo
- Publication number
- EP2796208A1 EP2796208A1 EP13164759.6A EP13164759A EP2796208A1 EP 2796208 A1 EP2796208 A1 EP 2796208A1 EP 13164759 A EP13164759 A EP 13164759A EP 2796208 A1 EP2796208 A1 EP 2796208A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- phi
- acoustic
- cos
- frequency
- power
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000002245 particle Substances 0.000 claims abstract description 21
- 230000003247 decreasing effect Effects 0.000 claims abstract description 7
- 230000010363 phase shift Effects 0.000 claims abstract description 6
- 230000000737 periodic effect Effects 0.000 claims abstract description 3
- 238000005259 measurement Methods 0.000 claims description 7
- 230000002401 inhibitory effect Effects 0.000 claims description 5
- 229920000642 polymer Polymers 0.000 claims description 4
- 230000007423 decrease Effects 0.000 claims description 2
- 238000001914 filtration Methods 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- 239000004945 silicone rubber Substances 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003094 perturbing effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000005368 silicate glass Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
- B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
- B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
- B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
- B06B1/0207—Driving circuits
- B06B1/0223—Driving circuits for generating signals continuous in time
- B06B1/0238—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave
- B06B1/0246—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave with a feedback signal
Definitions
- the present invention is related to a method for regulating the power and frequency of an acoustic field applied to an acoustic cell. More particularly, the present invention is related to the regulation of the control parameters used in an acoustic cell for separating particles such as biological cells in a liquid medium.
- the present invention is also related to an acoustic cell to be controlled by the method of the invention.
- a second strategy is based upon centrifugation.
- the developed systems are mechanically complex, and it is difficult to produce disposable parts that can provide continuous separation by centrifugation.
- acoustic separation was developed, to solve some of those problems.
- an acoustic field is applied to a resonant cavity, with the acoustic wave nodes and antinodes planes parallel to the direction of the liquid flow to be filtered.
- the particles are trapped in the antinodes planes and accumulate in those planes.
- the particles can then be collected by periodically stopping the acoustic field and reversing the flow or let the particles sediment by gravity to a collecting tank.
- the separation cell consist in a resonating cavity, comprising to opposed parallel plane surfaces, at least one of them being coupled to a piezoelectric transducer for producing the acoustic field.
- Document EP0633049 discloses such an acoustic cell, and the corresponding filtering method.
- Document EP0633049 describes how to calculate the theoretical resonance frequencies of typical cavities, but, such frequencies are usually not perfectly stable. For example, small dimensional variation of the plate distance, particle density, and fluid temperature are known to have an important impact on the sound speed and therefore on the resonance frequency.
- the frequency is usually adapted in a closed loop regulation, by maximising the power transfer to the fluid.
- the power needs to be sufficient at any time, so that the signal arising from the coupling with the filtered fluid is sufficient. Therefore, in such frequency tracking method, the power is permanently maintained higher than what is really needed for maintaining resonance conditions.
- An aim of the invention is to provide a method for continuously regulating both frequency and power applied to the piezoelectric transducer(s) of an acoustic cell wherein the injected power is minimised, and the resonance conditions are optimised.
- the method of the invention aims to provide a regulating method of both power and frequency applied to an acoustic cell adapted to continuously filter particles from a fluid having varying physical properties such as sound speed, compressibility and density.
- An aim of the invention is to provide a regulating method sufficiently robust to be used for controlling disposable acoustic cells having broad dimensional tolerances.
- the present invention is related to an iterative method for controlling an acoustic cell separating dispersed particles in a liquid medium, said acoustic cell comprising two opposed plates delimiting a resonating cavity filled with said liquid medium, at least one of the opposed surfaces comprising a piezoelectric transducer coupled to an electrical power generator for producing ultrasonic waves in said resonating cavity, said method comprising the steps of:
- the present invention is also related to a disposable acoustic cell for separating particles dispersed in a liquid medium, said acoustic cell comprising a polymeric housing and two opposed plates, said polymeric housing and said opposed plates defining an enclosure for receiving a liquid comprising particles to be separated from said liquid by an acoustic wave field, a piezoelectric transducer being fixed on at least one of said opposing plates for applying the acoustic wave field between said plates.
- Fig. 1 represents a side view of an example of acoustic cell according to the invention.
- Fig. 2 represents a top view of a cross section along the A-A' plane of the acoustic cell of fig.1
- Fig. 3 represents a schematic view of the regulating system of the invention.
- the present invention is related to a method for controlling the acoustic power and frequency injected in an acoustic cell.
- the acoustic cell comprises two opposed plates 5 defining a resonant cavity.
- An acoustic field is applied to the cavity by means of at least one piezoelectric 4 transducer fixed on the external surface of at least one of the opposed plates 5.
- the plate without transducer act as a mirror.
- a piezoelectric transducer is fixed on both plates. This allows the use of larger cells for a given injected power by each piezoelectric transducer, thereby reducing local heat dissipation.
- the plates have Knopp hardness HK 0.1/20 higher than 300, preferably higher than 480, and a density higher than the liquid density.
- This can be for example silicate glass, preferably borosilicate glass.
- the lateral sides of the acoustic cells comprises transparent viewing windows 3, for visual inspection of the filtering process (i.e. particles/cells agregation).
- An acoustic absorbing medium 10 is advantageously coated on those viewing window, in order to avoid complex reflexions on the windows, perturbing the resonant acoustic field. Silicone rubber is particularly adapted as absorbing medium.
- the inner dimensions of the cell, and the thickness of the plate are selected so that they are multiples of half of the wavelength of the injected sound.
- the properties of the liquid medium are varying around average values for example due to local temperature increase, particles density and composition of the liquid.
- acoustic cells preferably disposable.
- the dimensional tolerances are usually much broader than the tolerance obtained on expensive non disposable cells.
- the system can be calibrated once, reusing initially measured resonance frequency in subsequent uses. This is not realistically feasible in the case of disposable acoustic cells.
- the method of the invention does not only adapt the frequency to the resonance conditions, but also minimise the power injected in the liquid medium.
- phase shift phi between the applied electrical potential and the resulting electrical current is minimum.
- a convenient measurement of said phase shift phi is the measurement of the cosine of phi (cos(phi)), cos(phi) being maximum at resonance.
- the method of the invention is preferably controlled by a numerical processor controlling the frequency of a wave generator and the gain of an amplifier (power control) downstream of the wave generator, the piezoelectric transducer being connected to the output of the amplifier, as represented in Fig. 3 .
- an initial frequency is used to generate an acoustic field in the acoustic cell, and the gain of the amplifier is slowly increased until cos(phi) reaches a predetermined value.
- This step permits to obtain a sufficient signal to begin the tracking of the resonance frequency even in the case of badly known dimensions of the acoustic cell (disposable acoustic cell).
- the frequency is iteratively varied to increase cos(phi) and the power is decreased, so that in stable conditions, the resonance is maintained at the minimum feasible power input.
- the variation of the power and frequency is performed according to the following sequential steps:
- the determination of the direction of the gradient of cos(phi) can for example be determined by performing the minimum possible decrement of frequency, then measuring cos(phi), if cos(phi) has increased, this means that the gradient is negative and the frequency is decreased before getting back to step b, else, performing a double increment of the frequency, if cos(phi) has increased, it means that the gradient is positive, and the frequency is increased before getting back to step b. If cos(phi) has not increased in both directions (increment or decrement) the gradient is considered as being zero, and the frequency is maintained at its previous value before getting to step b.
- Sequences without acoustic field are used at periodical time in order to collect the particles agglutinated at the wave antinodes, the particles sedimenting when the acoustic field is stopped. Those stopping sequences are also used to perform a subsequent optimisation of the control parameters.
- the initial frequency applied to the transducers is determined by a dichotomic numerical method.
- a lag time of at least 1 ms, preferably 10ms, more preferably 100ms is used to let the system equilibrate after any frequency or power changes, before cos(phi) measurements.
- the method of the invention has been tested on disposable acoustic cells of the type represented in fig. 1 and 2 .
- the plates defining the resonant cavity where separated by 34mm.
- the plates themselves where made of glass plates of 1,2mm thickness.
- the plates dimensions where 41mm height and 31mm width.
- the wave generator was operated between 2,18 and 2.3MHz, the power control of the gain of the amplifier was performed by a step by step potentiometer, the power varying from 0 to 15W.
- the predetermined threshold of step b was 0,3538 and was identical to the predetermined value used in the initial step (see initial step hereabove).
- Typical operating cycle time is about 45s, separated by 5s lag time between each operating cycles.
- the system has shown robust behaviour in finding resonance conditions in disposable acoustic cells and in changing conditions, giving rise to better filtering conditions.
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatuses For Generation Of Mechanical Vibrations (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13164759.6A EP2796208A1 (fr) | 2013-04-22 | 2013-04-22 | Procédé pour commander une cellule acoustique |
| PCT/EP2014/057685 WO2014173745A2 (fr) | 2013-04-22 | 2014-04-16 | Procédé de commande d'une cellule acoustique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13164759.6A EP2796208A1 (fr) | 2013-04-22 | 2013-04-22 | Procédé pour commander une cellule acoustique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP2796208A1 true EP2796208A1 (fr) | 2014-10-29 |
Family
ID=48366114
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP13164759.6A Withdrawn EP2796208A1 (fr) | 2013-04-22 | 2013-04-22 | Procédé pour commander une cellule acoustique |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP2796208A1 (fr) |
| WO (1) | WO2014173745A2 (fr) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU174330U1 (ru) * | 2017-04-27 | 2017-10-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Сибирский государственный университет геосистем и технологий" (СГУГиТ) | Акустическая ловушка в поле стоячей волны на основе двух встречных пучков |
| CN109154516A (zh) * | 2016-03-30 | 2019-01-04 | 江森自控科技公司 | 液体检测系统 |
| US20220003653A1 (en) * | 2018-10-30 | 2022-01-06 | Siemens Healthcare Gmbh | Isovolumetric sphering of red blood cells |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10214718B2 (en) | 2013-07-01 | 2019-02-26 | University Of Massachusetts | Distributed perfusion bioreactor system for continuous culture of biological cells |
| CN119147476A (zh) * | 2024-09-18 | 2024-12-17 | 暨南大学 | 一种高灵敏宽量程的气体传感系统及相关测量方法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4689515A (en) * | 1985-09-30 | 1987-08-25 | Siemens Aktiengesellschaft | Method for operating an ultrasonic frequency generator |
| EP0633049A1 (fr) | 1993-05-11 | 1995-01-11 | Trampler, Felix, Dipl. Ing. | Méthode pour le traitement d'un liquide |
| US5711888A (en) * | 1993-05-11 | 1998-01-27 | Sonosep Biotech, Inc. | Multilayered piezoelectric resonator for the separation of suspended particles |
| US5892315A (en) * | 1996-06-26 | 1999-04-06 | Gipson; Lamar Heath | Apparatus and method for controlling an ultrasonic transducer |
| EP1195460A2 (fr) * | 2000-09-28 | 2002-04-10 | Kao Corporation | Dispositif et procédé de nettoyage par ultrasons |
| US20110254519A1 (en) * | 2008-12-02 | 2011-10-20 | Hiroshi Hasegawa | Ultrasonic generator and program writing method |
-
2013
- 2013-04-22 EP EP13164759.6A patent/EP2796208A1/fr not_active Withdrawn
-
2014
- 2014-04-16 WO PCT/EP2014/057685 patent/WO2014173745A2/fr not_active Ceased
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4689515A (en) * | 1985-09-30 | 1987-08-25 | Siemens Aktiengesellschaft | Method for operating an ultrasonic frequency generator |
| EP0633049A1 (fr) | 1993-05-11 | 1995-01-11 | Trampler, Felix, Dipl. Ing. | Méthode pour le traitement d'un liquide |
| US5711888A (en) * | 1993-05-11 | 1998-01-27 | Sonosep Biotech, Inc. | Multilayered piezoelectric resonator for the separation of suspended particles |
| US5892315A (en) * | 1996-06-26 | 1999-04-06 | Gipson; Lamar Heath | Apparatus and method for controlling an ultrasonic transducer |
| EP1195460A2 (fr) * | 2000-09-28 | 2002-04-10 | Kao Corporation | Dispositif et procédé de nettoyage par ultrasons |
| US20110254519A1 (en) * | 2008-12-02 | 2011-10-20 | Hiroshi Hasegawa | Ultrasonic generator and program writing method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109154516A (zh) * | 2016-03-30 | 2019-01-04 | 江森自控科技公司 | 液体检测系统 |
| US11162726B2 (en) | 2016-03-30 | 2021-11-02 | Johnson Controls Technology Company | Liquid detection system |
| RU174330U1 (ru) * | 2017-04-27 | 2017-10-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Сибирский государственный университет геосистем и технологий" (СГУГиТ) | Акустическая ловушка в поле стоячей волны на основе двух встречных пучков |
| US20220003653A1 (en) * | 2018-10-30 | 2022-01-06 | Siemens Healthcare Gmbh | Isovolumetric sphering of red blood cells |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014173745A2 (fr) | 2014-10-30 |
| WO2014173745A3 (fr) | 2015-03-26 |
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