EP2405959A4 - Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes - Google Patents

Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes

Info

Publication number: EP2405959A4
Authority: EP; European Patent Office
Prior art keywords: kits; apparatuses; methods; genetic material; living cells
Prior art date: 2009-03-13
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP10751463.0A

Other languages

German (de)

English (en)

Other versions

EP2405959A1 (fr

Inventor

Thomas Diefenbach

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Tufts University

Original Assignee

Tufts University

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2009-03-13

Filing date

2010-03-12

Publication date

2013-10-16

2010-03-12 Application filed by Tufts University filed Critical Tufts University

2012-01-18 Publication of EP2405959A1 publication Critical patent/EP2405959A1/fr

2013-10-16 Publication of EP2405959A4 publication Critical patent/EP2405959A4/fr

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Landscapes

Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Biotechnology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Chemical & Material Sciences (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Cell Biology (AREA)
Sustainable Development (AREA)
Mechanical Engineering (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

EP10751463.0A 2009-03-13 2010-03-12 Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes Withdrawn EP2405959A4 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US15985609P	2009-03-13	2009-03-13
PCT/US2010/027104 WO2010105135A1 (fr)	2009-03-13	2010-03-12	Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes

Publications (2)

Publication Number	Publication Date
EP2405959A1 EP2405959A1 (fr)	2012-01-18
EP2405959A4 true EP2405959A4 (fr)	2013-10-16

Family

ID=42728810

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP10751463.0A Withdrawn EP2405959A4 (fr)	2009-03-13	2010-03-12	Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes

Country Status (2)

Country	Link
EP (1)	EP2405959A4 (fr)
WO (1)	WO2010105135A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US9017991B2 (en)	2009-03-13	2015-04-28	Tufts University	Methods tip assemblies and kits for introducing material into cells
CN107058101B (zh)	2011-10-17	2021-06-01	麻省理工学院	细胞内传递
KR102386122B1 (ko)	2013-08-16	2022-04-14	메사추세츠 인스티튜트 오브 테크놀로지	세포로의 선택적 물질 전달
CA2964392A1 (fr)	2014-10-31	2016-05-06	Massachussetts Institute Of Technology	Administration de biomolecules en direction de cellules du systeme immunitaire
CA2964138C (fr)	2014-11-14	2023-11-14	Massachusetts Institute Of Technology	Acheminement de composes et de compositions dans des cellules active par des perturbations et un champ
AU2016206870B2 (en)	2015-01-12	2022-02-17	Massachusetts Institute Of Technology	Gene editing through microfluidic delivery
CA2988996A1 (fr)	2015-07-09	2017-01-12	Massachusetts Institute Of Technology	Administration de substances a des cellules anucleees

Citations (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5922687A (en) *	1995-05-04	1999-07-13	Board Of Trustees Of The Leland Stanford Junior University	Intracellular delivery of nucleic acids using pressure
EP0998945A1 (fr) *	1998-09-30	2000-05-10	Transgene S.A.	Utilisation du magnésium (mg2+) pour l'amélioration de la déliverence des gènes dans la thérapie génique
WO2003061831A2 (fr) *	2002-01-25	2003-07-31	Dormann Joerg Martin	Dispositif de prelevement d'echantillons et procede de determination quantifiable simultanee de germes infectieux
DE10361917A1 (de) *	2003-12-22	2005-07-21	Novosom Ag	Verfahren zur Transfektion von Zellen
US20070077306A1 (en) *	2003-12-26	2007-04-05	Toshihiro Akaike	Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (de) *	2007-01-31	2008-08-07	Universität Wien	Pipetteneinrichtung, Manipulationseinrichtung und Verfahren zur Manipulation biologischer Zellen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6982063B2 (en) *	2001-05-25	2006-01-03	Matrix Technologies Corp	Automated pipetting system

2010
- 2010-03-12 EP EP10751463.0A patent/EP2405959A4/fr not_active Withdrawn
- 2010-03-12 WO PCT/US2010/027104 patent/WO2010105135A1/fr not_active Ceased

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5922687A (en) *	1995-05-04	1999-07-13	Board Of Trustees Of The Leland Stanford Junior University	Intracellular delivery of nucleic acids using pressure
EP0998945A1 (fr) *	1998-09-30	2000-05-10	Transgene S.A.	Utilisation du magnésium (mg2+) pour l'amélioration de la déliverence des gènes dans la thérapie génique
WO2003061831A2 (fr) *	2002-01-25	2003-07-31	Dormann Joerg Martin	Dispositif de prelevement d'echantillons et procede de determination quantifiable simultanee de germes infectieux
DE10361917A1 (de) *	2003-12-22	2005-07-21	Novosom Ag	Verfahren zur Transfektion von Zellen
US20070077306A1 (en) *	2003-12-26	2007-04-05	Toshihiro Akaike	Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (de) *	2007-01-31	2008-08-07	Universität Wien	Pipetteneinrichtung, Manipulationseinrichtung und Verfahren zur Manipulation biologischer Zellen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2010105135A1 *

Also Published As

Publication number	Publication date
WO2010105135A1 (fr)	2010-09-16
EP2405959A1 (fr)	2012-01-18

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Legal Events

Date	Code	Title	Description
2011-12-16	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2012-01-18	17P	Request for examination filed	Effective date: 20111013
2012-01-18	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
2012-06-20	DAX	Request for extension of the european patent (deleted)
2013-10-16	A4	Supplementary search report drawn up and despatched	Effective date: 20130917
2013-10-16	RIC1	Information provided on ipc code assigned before grant	Ipc: C12M 1/42 20060101ALI20130911BHEP Ipc: C12M 3/00 20060101ALI20130911BHEP Ipc: A61M 5/00 20060101AFI20130911BHEP Ipc: C12N 15/89 20060101ALI20130911BHEP
2015-09-23	17Q	First examination report despatched	Effective date: 20150824
2017-05-08	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: EXAMINATION IS IN PROGRESS
2025-01-17	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2025-02-19	18D	Application deemed to be withdrawn	Effective date: 20241001