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EP2322277A1 - Puce microfluidique - Google Patents

Puce microfluidique Download PDF

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Publication number
EP2322277A1
EP2322277A1 EP10179869A EP10179869A EP2322277A1 EP 2322277 A1 EP2322277 A1 EP 2322277A1 EP 10179869 A EP10179869 A EP 10179869A EP 10179869 A EP10179869 A EP 10179869A EP 2322277 A1 EP2322277 A1 EP 2322277A1
Authority
EP
European Patent Office
Prior art keywords
recess
microfluidic
microfluidic chip
liquid
liquid reservoir
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP10179869A
Other languages
German (de)
English (en)
Other versions
EP2322277B1 (fr
Inventor
Jochen Rupp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2322277A1 publication Critical patent/EP2322277A1/fr
Application granted granted Critical
Publication of EP2322277B1 publication Critical patent/EP2322277B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the present invention relates to microfluidic chips, microfluidic fluid reservoirs, microfluidic kits and methods for their operation.
  • LOC Lab-on-a-chip
  • Liquids are usually stored in such systems on the chip itself or supplied to the chip via hoses. In this case, however, the actuation of the liquid and / or ensuring a sufficiently high sterility of the liquids can be problematic.
  • the present invention provides two-component solutions based on separation of the microfluidic chip and liquid reservoir and liquid actuation across a stretchable surface.
  • a separation of the chip and the liquid reservoir can have an advantageous effect on the storage and exchange of sensitive, in particular biologically active reagents containing liquids.
  • By a stretchable surface can thereby a simple and / or robust liquid actuation with an optionally high degree of sterility can be achieved.
  • the expandable membrane can be separated from the liquid side by the expandable membrane, in particular when using a suitably designed, later explained liquid reservoir, the Aktuleitersseite.
  • the Aktuleitersseite can be separated from the liquid side by the expandable membrane, in particular when using a suitably designed, later explained liquid reservoir, the Aktuleitersseite.
  • the Aktuleitersseite can be separated from the liquid side by the expandable membrane, in particular when using a suitably designed, later explained liquid reservoir, the Aktu michsseite.
  • the microfluidic chip can be a chip laboratory or laboratory-on-the-chip system or west pocket laboratory, for example for the analysis of water and / or blood.
  • the recess may in particular be designed such that a liquid reservoir in the recess, in particular liquid-tight and / or gas-tight, inserted and / or locked (clipped) and / or can be screwed.
  • the recess for receiving a liquid reservoir having a rigid wall area and an openable wall area or a liquid reservoir with a rigid wall area, a stretchable, in particular elastic, wall area and an openable wall area be formed.
  • the expandable membrane is preferably designed to contact the expandable wall region of a liquid reservoir mounted in the recess in the case of a liquid reservoir having a rigid and an openable wall region.
  • the liquid channel access is preferably designed to contact the openable wall region of a liquid reservoir mounted in the recess.
  • the microfluidic chip in particular the recess of the microfluidic chip, can furthermore have a groove and / or a spring / projection in order to communicate with the microfluidic through a corresponding spring / protrusion and / or groove of a microfluidic liquid reservoir, in particular a rigid wall region of a microfluidic liquid reservoir Liquid reservoir to be connected.
  • the expandable membrane can be stretched into a recess, in particular mounted liquid reservoir in volume displacement, in particular to move a liquid from the liquid reservoir into the liquid channel access.
  • the volume displaced by the expandable membrane can correspond to the moving volume of liquid.
  • the liquid container in addition to liquid still contains a gas, for example air, may - due to compression of the gas - the liquid volume moved be less than the displaced volume of the expandable membrane.
  • the actuator can be integrated in both the expandable membrane and separately to the expandable membrane, for example, adjacent to the expandable membrane, be formed.
  • the actuator may be a pneumatic actuator (for example, an actuator operated with external compressed air), a thermal actuator (for example, an actuator operated by gas generation), a piezoelectric actuator, and / or a mechanical actuator.
  • the actuator comprises a pressure channel adjacent to the expandable membrane.
  • the expandable membrane can be stretched in a simple manner under volume displacement in the recess.
  • the liquid channel access can be designed, for example, such that the liquid channel access opens when a liquid reservoir is received and / or closes when a liquid reservoir is removed.
  • the liquid channel access may be designed such that the liquid channel access opens on the basis of a mechanical mechanism when receiving a liquid reservoir and closes again when the liquid reservoir is removed. In this way, when replacing the liquid reservoir, it can be advantageously prevented that impurities enter the liquid channel and that liquid from the liquid channel flows back into the recess.
  • the liquid channel access may further or alternatively be designed like a valve.
  • the liquid channel access may comprise a perforated, elastic membrane whose perforations are liquid-impermeable under normal pressure and liquid-permeable upon application of a certain pressure, in particular due to expansion-induced enlargement.
  • microfluidic fluid reservoir for example, a partially or completely filled with a liquid, microfluidic fluid reservoir for insbesondre releasably receiving in a recess of a previously described microfluidic chip.
  • the liquid reservoir has a rigid wall region and an openable wall region, wherein the openable wall region is configured to contact the microfluidic chip in the recess, the expandable membrane and the liquid channel access of the microfluidic chip.
  • the liquid reservoir has a rigid wall region, a stretchable, in particular elastic, wall region and an openable wall region, wherein the expandable wall region is designed, in the state mounted in the recess of the microfluidic chip, the expandable membrane of the microfluidic chip to contact, in particular with the formation of a double membrane, and wherein the openable wall region is formed, in which in the Recess of the microfluidic chip mounted state to contact the liquid channel access of the microfluidic chip.
  • Such a closed, microfluidic liquid reservoir can advantageously be kept sterile more easily and thus the storage of sensitive liquids can be simplified.
  • the microfluidic fluid reservoir in particular the rigid wall region of the microfluidic fluid reservoir, can have a groove and / or a spring / projection in order to engage with the microfluidic chip through a corresponding spring / cantilever and / or groove of a microfluidic chip, in particular a recess of a microfluidic chip to be connected.
  • the openable wall region is designed in the form of a reversibly or irreversibly openable cover or in the form of a reversibly or irreversibly openable membrane.
  • a reversibly openable lid can be designed such that the lid opens on the basis of a mechanical mechanism, for example by moving the lid, when receiving the liquid reservoir in a recess of a microfluidic chip and when removing the liquid reservoir from a recess of a microfluidic chip closes again.
  • An irreversibly openable lid can be designed, for example, such that the lid opens on the basis of a mechanical mechanism, for example by removing or displacing the lid, when receiving the liquid reservoir in a recess of a microfluidic chip.
  • a reversibly openable membrane can be, for example, a perforated, elastic membrane whose perforations are liquid-impermeable under normal pressure and liquid-permeable when exposed to a certain pressure, in particular due to an expansion-related enlargement.
  • An irreversibly openable membrane may, for example, be a membrane which is destroyed during opening or when receiving the liquid reservoir in a recess of a microfluidic chip and thus opened.
  • the actuation side can advantageously be separated from the liquid side when using a suitably designed, later-described liquid reservoir, by the expandable wall region. This in turn ensures a high sterility of the fluids used.
  • liquids can be actuated in a simpler and / or more robust manner than in many known microfluidic chips.
  • the liquid reservoir can be opened and / or connected only directly before use, thereby simplifying storage and increasing the adaptability of the system.
  • the microfluidic chip can here also be a chip laboratory or laboratory-on-the-chip system or west pocket laboratory, for example for the analysis of water and / or blood.
  • the recess can also be formed in this case such that a liquid reservoir in the recess, in particular liquid-tight and / or gas-tight, inserted and / or locked (clipped) and / or can be screwed.
  • the microfluidic chip in particular the recess of the microfluidic chip, may in this case also have a groove and / or a spring / projection in order to engage with a corresponding spring / cantilever and / or groove of a microfluidic liquid reservoir, in particular a rigid wall region of a microfluidic liquid reservoir microfluidic fluid reservoir to be connected.
  • the actuator can also be a pneumatic actuator (for example an actuator operated with external compressed air), a thermal actuator (for example an actuator operated by gas generation), a piezoelectric actuator and / or a mechanical actuator.
  • the actuator also comprises a pressure channel adjacent to the recess.
  • a pressure channel adjacent to the recess.
  • a stretchable, in particular elastic, wall region of a liquid reservoir mounted in the recess can be stretched in a simple manner into the liquid reservoir with volume displacement.
  • the actuator can be designed to contact a stretchable, in particular elastic, wall region of a liquid reservoir mounted in the recess.
  • the liquid channel access may, for example, be designed such that the liquid channel access opens when a liquid reservoir is received and / or closes when a liquid reservoir is removed.
  • the liquid channel access may be configured such that the liquid channel access opens on the basis of a mechanical mechanism when receiving a liquid reservoir and when Removal of the liquid reservoir closes again. In this way, when replacing the liquid reservoir, it can be advantageously prevented that impurities enter the liquid channel and that liquid from the liquid channel flows back into the recess.
  • the liquid channel access may further or alternatively be designed like a valve.
  • the liquid channel access may comprise a perforated, elastic membrane whose perforations are liquid-impermeable under normal pressure and liquid-permeable upon application of a certain pressure, in particular due to expansion-induced enlargement.
  • the liquid channel access is preferably designed to contact the openable wall region of a liquid reservoir mounted in the recess.
  • Another object of the present invention is a microfluidic fluid reservoir, for example, a partially or completely filled with a liquid, microfluidic fluid reservoir, an alternative embodiment of the invention, which is designed for releasably receiving in particular in a recess of a microfluidic chip of the alternative embodiment of the invention.
  • a microfluidic liquid reservoir of the alternative embodiment according to the invention has a rigid wall region, an extensible, in particular elastic, wall region and an openable wall region, wherein the expandable wall region is designed, in the state mounted in the recess of the microfluidic chip, the actuator of the microfluidic chip and wherein the openable wall region is designed to contact the liquid channel access of the microfluidic chip in the state mounted in the recess of the microfluidic chip.
  • microfluidic liquid reservoir can advantageously be kept sterile more easily and thereby the storage of sensitive liquids can be simplified.
  • the microfluidic liquid reservoir in particular the rigid wall region of the microfluidic liquid reservoir, may also have a groove and / or a spring / projection in order to be replaced by a corresponding one Spring / projection and / or groove of a microfluidic chip, in particular a recess of a microfluidic chip to be connected to the microfluidic chip.
  • the openable wall region is also formed here in the form of a reversibly or irreversibly openable lid or in the form of a reversibly or irreversibly openable membrane.
  • a lid that can be reversibly opened can be designed in such a way that the lid opens on the basis of a mechanical mechanism, for example by moving the lid, when receiving the liquid reservoir in a recess of a microfluidic chip and when removing the liquid reservoir from a recess of a microfluidic chips closes again.
  • An irreversibly openable lid can also be designed, for example, in such a way that the lid opens on the basis of a mechanical mechanism, for example by removing or displacing the lid, when receiving the liquid reservoir in a recess of a microfluidic chip.
  • a reversibly openable membrane may in this case also be, for example, a perforated, elastic membrane whose perforations are impervious to liquid under normal pressure and permeable to liquid when exposed to a certain pressure, in particular due to an expansion-related enlargement.
  • An irreversibly openable membrane may in this case also be a membrane which is destroyed during opening or when receiving the liquid reservoir in a recess of a microfluidic chip and thus opened.
  • Another object of the present invention is a microfluidic kit / system, which is a microfluidic chip of the first described embodiment of the invention and a microfluidic fluid reservoir of the first described embodiment of the invention or a microfluidic Chip of the alternative, inventive embodiment and a microfluidic liquid reservoir of the alternative embodiment according to the invention comprises.
  • the two parts can either be made separately from each other and filled or produced together.
  • FIG. 1a shows a first embodiment of a microfluidic liquid reservoir 2 according to the invention.
  • FIG. 1a shows that the liquid reservoir 2 has a rigid wall region 9 and an openable wall region 10 and is completely filled with a liquid 8.
  • the openable wall region 10 is designed in the form of a reversibly or irreversibly openable cover 10.
  • FIG. 1a shows that the rigid wall region 9 of the microfluidic liquid reservoir 2 has a spring / projection 11 in order to be connected to the microfluidic chip 1 by a corresponding groove 12 of the recess 3 of the microfluidic chip 1 described below.
  • FIG. 1b 1 shows a first embodiment of a microfluidic chip 1 according to the invention, which comprises a recess 3 for receiving a liquid reservoir 2, an expandable membrane 4 adjoining the recess 3, an actuator 5 and a liquid channel access 6 adjoining the recess 3.
  • FIG. 1b further shows that the actuator 5 comprises a, adjacent to the expandable membrane 4 pressure channel 5.
  • the expandable membrane 4 can be stretched into the recess 3 by the actuator 5 or pressure channel 5 with volume displacement.
  • the microfluidic chip 1 can be constructed from a structured base substrate 1a and a structured support substrate 1b, the expandable membrane 4 being arranged between the base substrate and the support substrate 1b.
  • Figure 1c shows a first embodiment of a microfluidic kit according to the invention in the unaktu faced state.
  • Figure 1c illustrates that the structure is based on the fact that the in FIG. 1a shown microfluidic liquid reservoir 2 in the recess 3 of the in FIG. 1b introduced microfluidic chip 1 according to the invention introduced and thereby mounted.
  • Figure 1c further illustrates that the in FIG. 1a shown liquid reservoir 2 for receiving in the recess 3 of in FIG. 1b 1, wherein the openable wall region 10 of the liquid reservoir 2-in the state of the liquid reservoir 2 mounted in the recess 3 of the microfluidic chip 1-contacts the expandable membrane 4 and the liquid channel access 6 of the microfluidic chip 1.
  • the rigid wall regions 9 at least partially contact the lateral walls of the recess 3.
  • Figure 1d shows the kit Figure 1c in the actuated state.
  • Figure 1d illustrates that by applying a pressure to the pressure channel 5, the expandable membrane 4 can be stretched under volume displacement in the liquid reservoir 2 mounted in the recess 3, in order in this way a liquid 8 from the liquid reservoir 2 through the liquid channel access 6 into a liquid channel. 7 to move.
  • FIG. 2a shows a second embodiment of a microfluidic fluid reservoir according to the invention 2,2 '.
  • a configured liquid reservoir can advantageously be used both with the previously described first embodiment of a microfluidic chip 1 according to the invention and with the second embodiment of a microfluidic chip 1 'according to the invention described in detail below.
  • FIG. 2a shows that this liquid reservoir 2,2 'a rigid wall portion 9,9' has a stretchable, in particular elastic, wall portion 10a, 10a 'and an openable wall portion 10b, 10b' and is completely filled with a liquid 8.
  • the openable wall portion 10b, 10b ' is designed to the
  • the expandable wall region 10a, 10a ' is designed to contact the expandable membrane 4 of a chip 1 according to the invention of the first embodiment or the actuator 5' of a chip 1 'according to the invention described later in detail second embodiment.
  • two expandable surfaces namely the expandable membrane 4 of the microfluidic chip 1 and the expandable wall region 10a of the microfluidic liquid reservoir, are then adjacent to one another and form a "double membrane". Further shows FIG.
  • FIG. 2b shows a second embodiment of a microfluidic chip 1 'according to the invention.
  • FIG. 2b shows that the microfluidic chip 1 'while a recess 3' for receiving a in FIG. 2a shown liquid reservoir 2,2 'with a rigid wall portion 9,9', a stretchable, in particular elastic, wall portion 10,10a 'and an openable wall portion 10b, 10b', an 'adjacent to the recess 3', actuator 5 'and a, to the recess 3 'adjacent, liquid channel access 6' includes.
  • the actuator 5 ' is designed to contact a stretchable wall region 10a, 10a' of a liquid reservoir 2,2 'mounted in the recess 3'. Further shows FIG.
  • FIG. 2b in that the actuator 5 'comprises a pressure channel 5' adjoining the recess 3 '. Further shows FIG. 2b in that by applying a pressure to the pressure channel 5 ', an expandable wall region 10a, 10a,' of a liquid reservoir 2,2 'mounted in the recess can be stretched in a simple manner into the liquid reservoir 2,2' with volume displacement.
  • Figure 2c shows a second embodiment of a microfluidic kit according to the invention in the unaktu faced state.
  • Figure 2c illustrates that the structure is based on the fact that the in FIG. 2a shown, microfluidic liquid reservoir 2,2 'in the recess 3' of the in FIG. 2b shown microfluidic chip 1 'according to the invention introduced and thereby mounted.
  • Figure 2d shows the kit Figure 2c in the actuated state.
  • Figure 2d illustrates that by applying a pressure to the pressure channel 5 'of the expandable wall portion 10a, 10a' can be stretched under volume displacement in the mounted in the recess 3 'liquid reservoir 2,2' into, in this way a liquid 8 from the liquid reservoir. 2 2 'through the liquid channel access 6' into a liquid channel 7 'to move.
  • Figure 2d further shows that the openable wall portion 10b, 10b 'was formed in the form of an irreversible, openable membrane, which was destroyed during the actuation of the liquid 8 and thus opened.
  • FIG. 3a shows an enlarged, schematic, perspective view of the first embodiment of the inventive microfluidic chip 1 with a capped by a stretchable membrane 4 pressure chamber fifth FIG. 3a illustrates that the recess 3 of the microfluidic chip 1 has a groove 12 to be connected by a corresponding spring / projection 11 of a microfluidic liquid reservoir 2 with the microfluidic liquid reservoir 2.
  • FIG. 3b shows a larger section of the in FIG. 3a shown microfluidic chips 1 after insertion of a microfluidic liquid reservoir 2 with a spring / projection 11 in the recess 3 and the groove 12th

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Micromachines (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
EP10179869.2A 2009-10-14 2010-09-27 Puce microfluidique Active EP2322277B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102009045685A DE102009045685A1 (de) 2009-10-14 2009-10-14 Mikrofluidischer Chip

Publications (2)

Publication Number Publication Date
EP2322277A1 true EP2322277A1 (fr) 2011-05-18
EP2322277B1 EP2322277B1 (fr) 2018-04-04

Family

ID=43446934

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10179869.2A Active EP2322277B1 (fr) 2009-10-14 2010-09-27 Puce microfluidique

Country Status (4)

Country Link
US (1) US20110086433A1 (fr)
EP (1) EP2322277B1 (fr)
DE (1) DE102009045685A1 (fr)
ES (1) ES2676152T3 (fr)

Cited By (1)

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EP2796200A3 (fr) * 2013-04-26 2014-11-19 Robert Bosch Gmbh Procédé et dispositif destinés à la fabrication d'une cartouche d'analyse microfluidique

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KR101337136B1 (ko) * 2012-05-30 2013-12-05 충북대학교 산학협력단 다이어프램 피더와 통합된 액적 기반 미세유체 시스템
DE102012222719A1 (de) 2012-12-11 2014-06-12 Robert Bosch Gmbh Folienbeutel zum Bevorraten eines Fluids und Vorrichtung zum Bereitstellen eines Fluids
DE102013201297A1 (de) 2013-01-28 2014-07-31 Robert Bosch Gmbh Einheit zum Bevorraten eines Fluids und Verfahren zur Herstellung einer Einheit zum Bevorraten eines Fluids
DE102013207193A1 (de) * 2013-04-22 2014-10-23 Robert Bosch Gmbh Mikrohydraulisches System, insbesondere zum Einsatz in planaren Mikrofluidiklaboren
DE102013209866B4 (de) * 2013-05-28 2021-11-04 Robert Bosch Gmbh Vorrichtung mit vorgegebener Fluidverdrängung
EP2905079A1 (fr) * 2014-02-10 2015-08-12 Robert Bosch Gmbh Dispositif de stockage préalable d'un fluide dans un système micro-fluidique, procédé de fonctionnement et procédé de fabrication d'un tel dispositif
DE102015226417A1 (de) * 2015-12-22 2017-06-22 Robert Bosch Gmbh Mikrofluidische Vorrichtung, Verfahren zum Herstellen und Verfahren zum Betreiben einer mikrofluidischen Vorrichtung
DE102017220525A1 (de) * 2017-11-17 2019-05-23 Robert Bosch Gmbh Adapter zur Probeneingabe in eine mikrofluidische Vorrichtung
FR3074069A1 (fr) * 2017-11-28 2019-05-31 Commissariat A L'energie Atomique Et Aux Energies Alternatives Dispositif d'injection d'un echantillon fluidique
DE102020210276A1 (de) 2020-08-13 2022-02-17 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Vorrichtung und Verfahren zu ihrer Herstellung
DE102023212142A1 (de) 2023-12-04 2025-06-05 Robert Bosch Gesellschaft mit beschränkter Haftung Vorrichtung und Verfahren zur Kultivierung und Testung von Gewebeschnitten

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US20080050830A1 (en) * 2006-05-10 2008-02-28 The Board Of Regents Of The University Of Texas System Detecting multiple types of leukocytes
WO2009105711A1 (fr) * 2008-02-21 2009-08-27 Decision Biomarkers, Inc. Essais ayant pour base un écoulement de liquide sur des réseaux

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2796200A3 (fr) * 2013-04-26 2014-11-19 Robert Bosch Gmbh Procédé et dispositif destinés à la fabrication d'une cartouche d'analyse microfluidique
US10295441B2 (en) 2013-04-26 2019-05-21 Robert Bosch Gmbh Method and device for producing a microfluidic analysis cartridge

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US20110086433A1 (en) 2011-04-14
EP2322277B1 (fr) 2018-04-04
DE102009045685A1 (de) 2011-04-21
ES2676152T3 (es) 2018-07-17

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