[go: up one dir, main page]

EP1877773B1 - Appareil et procede pour enduire des substrats pour mettre en evidence un analyte par depistage d'affinite - Google Patents

Appareil et procede pour enduire des substrats pour mettre en evidence un analyte par depistage d'affinite Download PDF

Info

Publication number
EP1877773B1
EP1877773B1 EP06724515.9A EP06724515A EP1877773B1 EP 1877773 B1 EP1877773 B1 EP 1877773B1 EP 06724515 A EP06724515 A EP 06724515A EP 1877773 B1 EP1877773 B1 EP 1877773B1
Authority
EP
European Patent Office
Prior art keywords
liquid
substrates
atomized
coating
carrier substrates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP06724515.9A
Other languages
German (de)
English (en)
Other versions
EP1877773A1 (fr
Inventor
Eginhard Schick
Dominic Utinger
Claudio Calonder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Intellectual Property GmbH
Original Assignee
Bayer Intellectual Property GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Intellectual Property GmbH filed Critical Bayer Intellectual Property GmbH
Publication of EP1877773A1 publication Critical patent/EP1877773A1/fr
Application granted granted Critical
Publication of EP1877773B1 publication Critical patent/EP1877773B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B17/00—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups
    • B05B17/04—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods
    • B05B17/06—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations
    • B05B17/0607—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers
    • B05B17/0615—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers spray being produced at the free surface of the liquid or other fluent material in a container and subjected to the vibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Definitions

  • the inventive method is also characterized by the possibility of simultaneous coating of a large number of carrier substrates in a common, appropriately sized container and ease of automation and is easily feasible even for untrained personnel.
  • the liquid volumes to be used for coating the carrier substrates are of similar order of magnitude as in the case of the spray method.
  • a coating system according to the invention to produce geometrically structured coatings in a coating method according to the invention by optionally sequential atomization of one or more optionally different liquids.
  • the prerequisite for producing reproducible coated regions on the carrier substrates in their geometry is that areas of the carrier substrate that are not to be coated in each case are covered fluidically by a corresponding suitable mask, so that no mist droplets reach the areas not to be coated.
  • the coating apparatus additionally comprises provisions for controlling and / or regulating the pressure within the liquid container during the coating process.
  • luminescence in this application refers to the spontaneous emission of photons in the ultraviolet to infrared range after optical or non-optical, such as electrical or chemical or biochemical or thermal excitation.
  • chemiluminescence, bioluminescence, electroluminescence and in particular fluorescence and phosphorescence are included under the term “luminescence”. Fluorescence and phosphorescence are particularly preferred forms of luminescence.
  • the discrete or continuous waveguiding regions of the carrier substrates to be coated are in contact with one or more grating structures (c), which enable the coupling of excitation light or measuring light into waveguiding layers of said carrier substrates, and / or to one or more grating structures (c '), which enable the decoupling of excitation light or measuring light from waveguiding layers of said carrier substrates are, wherein in the case of simultaneously present on a carrier substrate grating structures (c) and (c') may have the same or different grating periods.
  • a mist eliminator to be used may also be permeable to drops up to a defined size. This can be technically realized, for example, by virtue of the fact that the mist eliminator comprises a fine-meshed net, with the mesh spacing of which the maximum size of drops to be passed is determined.
  • the coating apparatus additionally comprises at least one gas inlet via which a gas is introduced into the liquid container, which gas mixes with the generated mist.
  • the apparatus may additionally include one or more outlets for venting gas and / or mist.
  • the liquid container of the coating apparatus is closed except for optional inlets for gas and optional additional outlets for gas and / or mist.
  • FIG Fig. 1 A schematic representation of a coating apparatus according to the invention is shown in FIG Fig. 1 shown.
  • the areas of unprotected areas of specific binding partners are "passivated” by carrier substrates prepared for an affinity detection method, ie a "passivation layer” is applied in these areas.
  • the background signals are determined from the measured signal intensities between the spots.
  • four further circular areas are defined as evaluation areas for background signal determination per spot, which are preferably arranged in the middle between between adjacent spots.
  • the average background signal intensity is determined, for example, as the arithmetic mean of the pixel values (signal intensities) within an AOI selected for this purpose.
  • the average net signal intensity from the measurement areas (spots) is then calculated as the difference between the local average gross and local mean background signal intensity of the respective spot.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (9)

  1. Appareil destiné à l'enduction de substrats porteurs pour la détection d'un ou de plusieurs analytes dans un procédé de détection par affinité, comprenant :
    - un récipient (1) fermé hormis d'optionnelles entrées de gaz (5) et sorties de gaz (6), destiné à recevoir un liquide (7) à nébuliser avec des substances contenues dans celui-ci, à déposer sur au moins une surface desdits supports porteurs ainsi qu'un volume de brouillard engendré au-dessus du liquide dans l'état de fonctionnement,
    - un actionneur (3) destiné à déclencher le processus de nébulisation, immergé dans le liquide (7) à nébuliser au-dessous d'un
    - support (2), le support dans le récipient fermé étant dans le volume de brouillard engendré et les substrats porteurs ne se trouvant en aucun contact avec la surface du liquide à nébuliser,
    caractérisé en ce que le support sert à la réception horizontale par rapport à la surface du liquide à nébuliser et au logement des substrats porteurs pendant le processus d'enduction.
  2. Appareil selon la revendication 1, caractérisé en ce que dans l'état de fonctionnement ledit actionneur est immergé dans le liquide à nébuliser.
  3. Appareil selon l'une quelconque des revendications 1 - 2, caractérisé en ce qu'il comprend en outre un séparateur de gouttes.
  4. Appareil selon la revendication 3, caractérisé en ce que le séparateur de gouttes laisse passer les gouttes jusqu'à une grosseur définie et comprend un filet à mailles fines, par l'ouverture de maille duquel est fixée la taille la taille maximale des gouttes qu'il laisse passer.
  5. Appareil selon l'une quelconque des revendications 1 - 4, caractérisé en ce que il comprend en outre des moyens destinés à engendrer dans l'environnement desdits substrats porteurs une répartition uniforme du brouillard engendré et destiné à se déposer sur les substrats porteurs.
  6. Appareil selon l'une quelconque des revendications 1 - 5, caractérisé en ce que il comprend en outre des moyens pour le contrôle et/ou la régulation de la température du liquide à nébuliser et/ou de certaines ou de l'ensemble des parois du réservoir de liquide.
  7. Appareil selon l'une quelconque des revendications 1 - 6, caractérisé en ce que le support de l'appareil d'enduction, destiné à la réception et/ou au logement des substrats porteurs pendant le processus d'enduction est thermostatable.
  8. Procédé destiné à l'enduction de substrats porteurs pour la détection d'un ou de plusieurs analytes dans un procédé de détection par affinité, caractérisé en ce
    - qu'on insère lesdits substrats porteurs à revêtir dans un support d'un appareil d'enduction selon l'une quelconque des revendications 1 - 7,
    - on nébulise du liquide contenu dans le réservoir de liquide dudit appareil d'enduction et
    - à partir du brouillard engendré un dépôt de substances contenues dans le liquide nébulisé s'effectue sur les substrats porteurs à revêtir,
    les substrats porteurs ne se trouvant en aucun contact avec la surface du liquide à nébuliser.
  9. Substrat porteur destiné à la détection d'un ou de plusieurs analytes dans un procédé de détection par affinité, le substrat porteur étant plan, comprenant des zones de mesure séparées spatialement, appliquées directement sur le substrat porteur ou à l'aide d'une couche conférant de l'adhérence appliquée sur le substrat, les zones libres entre et à l'intérieur des zones de mesure étant recouvertes d'une couche de passivation, caractérisé en ce que ladite couche de passivation est produite par un procédé d'enduction selon la revendication 8.
EP06724515.9A 2005-04-26 2006-04-22 Appareil et procede pour enduire des substrats pour mettre en evidence un analyte par depistage d'affinite Not-in-force EP1877773B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH7352005 2005-04-26
PCT/EP2006/003726 WO2006114249A1 (fr) 2005-04-26 2006-04-22 Appareil et procede pour enduire des substrats pour mettre en evidence un analyte par depistage d'affinite

Publications (2)

Publication Number Publication Date
EP1877773A1 EP1877773A1 (fr) 2008-01-16
EP1877773B1 true EP1877773B1 (fr) 2014-10-15

Family

ID=36808724

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06724515.9A Not-in-force EP1877773B1 (fr) 2005-04-26 2006-04-22 Appareil et procede pour enduire des substrats pour mettre en evidence un analyte par depistage d'affinite

Country Status (9)

Country Link
US (1) US9050615B2 (fr)
EP (1) EP1877773B1 (fr)
JP (1) JP5002584B2 (fr)
CN (1) CN101208599B (fr)
AU (1) AU2006239534B2 (fr)
CA (1) CA2605750A1 (fr)
DK (1) DK1877773T3 (fr)
ES (1) ES2525324T3 (fr)
WO (1) WO2006114249A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2485564A (en) * 2010-11-18 2012-05-23 Rolls Royce Plc Luminescent surface coating for inspection
WO2013001431A1 (fr) 2011-06-30 2013-01-03 Koninklijke Philips Electronics N.V. Examens multiples d'un échantillon
US10545141B2 (en) 2014-01-10 2020-01-28 Konica Minolta, Inc. Immunoassay method and immunoassay system
DE102014112625A1 (de) * 2014-09-02 2016-03-03 Schmid Energy Systems Gmbh Großflächen-Ultraschallverdampfer
EP3229977A4 (fr) * 2014-12-08 2019-02-27 University of Virginia Patent Foundation, D/B/A University of Virginia Compositions et procédés pour lier des colles, des adhésifs et des revêtements à des surfaces
CH712376A1 (de) 2016-04-19 2017-10-31 Camag Derivatisierungsgerät und -verfahren.
CN106198952A (zh) * 2016-07-01 2016-12-07 清华大学 一种抑制核酸分子对传感界面非特异性吸附的封闭方法
CN107807133A (zh) * 2017-11-08 2018-03-16 苏州安路特汽车部件有限公司 一种雾化渗透探伤装置
WO2020223368A1 (fr) * 2019-04-29 2020-11-05 Nautilus Biotechnology, Inc. Procédés et systèmes de détection de molécule unique intégrée sur puce
CN115147617B (zh) * 2022-09-06 2022-11-22 聊城集众环保科技有限公司 基于计算机视觉的污水处理智能监控方法
CN115837944A (zh) * 2023-02-16 2023-03-24 太原理工大学 一种电厂烟气注入采空区防灾封存的气体监测装置

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5955367A (ja) * 1982-09-21 1984-03-30 Tdk Corp 静電防止剤塗布装置

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3514847A (en) * 1966-05-10 1970-06-02 Gen Dynamics Corp Process for making photoconductive matrices
WO1984001031A1 (fr) 1982-08-27 1984-03-15 Roger Philip Ekins Mesure de la concentration d'un produit
JPS6146028A (ja) * 1984-08-10 1986-03-06 Fujitsu Ltd レジスト塗布装置
US5807755A (en) 1987-08-06 1998-09-15 Multilyte Limited Determination of ambient concentrations of several analytes
GB8803000D0 (en) 1988-02-10 1988-03-09 Ekins Roger Philip Determination of ambient concentrations of several analytes
JPH01115450A (ja) * 1987-10-28 1989-05-08 Seiko Epson Corp 潤滑層の形成方法
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JPH03296460A (ja) * 1990-04-13 1991-12-27 Fujitsu General Ltd フラックス塗付装置
FR2669246A1 (fr) * 1990-11-16 1992-05-22 Centre Nat Rech Scient Procede sol-gel de depot de couches minces par pulverisation ultrasonore.
EP0524301B1 (fr) * 1991-02-11 2002-07-24 Thermo Biostar, Inc. Systeme et procede d'analyse immunologique elllipsometrique comportant un dispositif de detection a couches minces
JPH06134367A (ja) * 1992-04-10 1994-05-17 Murata Mfg Co Ltd 電子部品の外装構造
IT1271969B (it) * 1993-03-04 1997-06-10 Mario Bonzi Dispositivo per la umidificazione ed ionizzazione dell'aria
GB9326450D0 (en) 1993-12-24 1994-02-23 Multilyte Ltd Binding assay
CA2190362A1 (fr) 1994-05-27 1995-12-07 Gert L. Duveneck Procede de detection d'une luminescence a excitation evanescente
PL323257A1 (en) 1995-05-12 1998-03-16 Ciba Geigy Ag Detection platform for simultaneously detecting plurality of analytes using hypercritically excited luminescence
WO1997002856A1 (fr) * 1995-07-10 1997-01-30 A & D Company, Limited Atomiseur pratique
KR0144065B1 (ko) * 1995-08-30 1998-08-01 배순훈 히터식 초음파 가습기
US6143063A (en) * 1996-03-04 2000-11-07 Symetrix Corporation Misted precursor deposition apparatus and method with improved mist and mist flow
EP0922214A1 (fr) 1996-08-29 1999-06-16 Novartis AG Capteur optique chimique/biochimique
US6045864A (en) * 1997-12-01 2000-04-04 3M Innovative Properties Company Vapor coating method
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
WO2000065352A1 (fr) 1999-04-28 2000-11-02 Eidgenossisch Technische Hochschule Zurich Revetements polyioniques dans des dispositifs d'analyse et de detection
EP1207955A1 (fr) * 1999-08-27 2002-05-29 Picogram, Inc. Procede et dispositif permettant de realiser des operations sur des microsites charges
US6510263B1 (en) 2000-01-27 2003-01-21 Unaxis Balzers Aktiengesellschaft Waveguide plate and process for its production and microtitre plate
US20020064482A1 (en) 2000-02-02 2002-05-30 Tisone Thomas C. Method and apparatus for developing DNA microarrays
AU2001262178A1 (en) 2000-04-14 2001-10-30 Zeptosens Ag Grid-waveguide structure for reinforcing an excitation field and use thereof
AU2001226796A1 (en) 2000-05-06 2001-11-26 Zeptosens Ag Grating optical waveguide structure for multi-analyte determinations and the usethereof
WO2002021110A1 (fr) 2000-09-04 2002-03-14 Zeptosens Ag Systeme et procedes pour determiner plusieurs analytes
EP1373875A2 (fr) 2001-04-02 2004-01-02 Zeptosens AG Structure optique d'excitation multiphoton et son utilisation
AU2002357547A1 (en) 2001-12-13 2003-06-23 Zeptosens Ag Optically transparent substrate for a maldi measuring system and the use thereof
WO2004023142A1 (fr) 2002-09-03 2004-03-18 Zeptosens Ag Plate-forme analytique et procede d'identification avec des substances d'analyse a identifier dans un echantillon, se presentant sous forme de partenaires de liaison specifiques immobilises
DE10320312A1 (de) 2003-05-06 2004-12-02 Friz Biochem Gmbh Substrat als Träger von Ligaten
JP4318978B2 (ja) * 2003-07-29 2009-08-26 独立行政法人科学技術振興機構 高分子固定化基板の製造方法およびこの方法により得られる高分子固定化基板

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5955367A (ja) * 1982-09-21 1984-03-30 Tdk Corp 静電防止剤塗布装置

Also Published As

Publication number Publication date
US9050615B2 (en) 2015-06-09
ES2525324T3 (es) 2014-12-22
DK1877773T3 (en) 2015-01-19
AU2006239534B2 (en) 2012-05-31
AU2006239534A1 (en) 2006-11-02
EP1877773A1 (fr) 2008-01-16
CA2605750A1 (fr) 2006-11-02
CN101208599A (zh) 2008-06-25
US20090311773A1 (en) 2009-12-17
JP5002584B2 (ja) 2012-08-15
CN101208599B (zh) 2014-12-10
WO2006114249A1 (fr) 2006-11-02
JP2008539399A (ja) 2008-11-13

Similar Documents

Publication Publication Date Title
EP1327135B1 (fr) Systeme et procedes pour determiner plusieurs analytes
EP1237654B9 (fr) Ensemble de recipients pour echantillons et son utilisation dans la determination de plusieurs analytes
DE60003171T2 (de) Methoden zur miniaturisierten zellenanordnung und auf zellen basierendes screening gerät
EP1274986B1 (fr) Dispositif et procede de determination de plusieurs substances a analyser
WO2001092870A2 (fr) Kit et procede pour la detection d'une pluralite d'analytes
EP1877773B1 (fr) Appareil et procede pour enduire des substrats pour mettre en evidence un analyte par depistage d'affinite
DE60222378T2 (de) Fluoreszenzanalseelement mit der Verwendung einer metallischen Nanovertiefung und Verfahren zur Herstellung desselben
EP1334361A2 (fr) Kit et procede permettant la detection d'analytes multiples avec des mesures permettant la referenciation de la densite d'elements de reconnaissance immobilises
WO2001026797A2 (fr) Traitement d'echantillons dans des solutions presentant une faible surface de contact de paroi definie
EP1941276A1 (fr) Procédé pour la détermination d une ou plusieurs substances à analyser dans des échantillons de composition complexe d'origine biologique et son application
EP1556695A2 (fr) Plate-forme analytique et procede d'identification par des analytes a deceler dans un echantillon eventuellement apres fractionnement et se presentant sous forme de partenaires de liaison specifiques
EP1506403A2 (fr) Trousse de mise au point de dosage et d'analyses en serie
WO2010115530A1 (fr) Dispositif et procédé pour la détection et pour l'analyse quantitative d'analytes, notamment de mycotoxines
WO2002046756A1 (fr) Kit et procede de detection d'analytes multiples comprenant des mesures de referenciation a resolution locale d'une intensite de lumiere d'excitation
EP1561109A1 (fr) Plate-forme analytique et procede d'identification avec des substances d'analyse a identifier dans un echantillon, se presentant sous forme de partenaires de liaison specifiques immobilises
EP0905515B1 (fr) Méthode pour la mésure analytique et son usage
WO2003056335A2 (fr) Reseau de micropuits permettant de trier ou separer des cellules en suspension dans un fluide en ecoulement en fonction de leur taille et procede pour analyser l'activite fonctionnelle de cellules individuelles
DE102004021904B4 (de) Verfahren und Vorrichtung zur Erzeugung einer Analyseanordnung mit diskreten, separaten Messbereichen zur biologischen, biochemischen oder chemischen Analyse
EP1603673A1 (fr) Procede pour impregner, de maniere controlee, des zones predeterminees avec de faibles volumes de liquide, element de recouvrement de substrat et chambre d'ecoulement
EP1963441A1 (fr) Polyelektrolytes monocouches ou multicouches pour des transducteurs de signaux optiques

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20071126

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

RIN1 Information on inventor provided before grant (corrected)

Inventor name: UTINGER, DOMINIC

Inventor name: SCHICK, EGINHARD

Inventor name: CALONDER, CLAUDIO

RIN1 Information on inventor provided before grant (corrected)

Inventor name: CALONDER, CLAUDIO

Inventor name: SCHICK, EGINHARD

Inventor name: UTINGER, DOMINIC

DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: BAYER INTELLECTUAL PROPERTY GMBH

17Q First examination report despatched

Effective date: 20130617

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20140708

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 691904

Country of ref document: AT

Kind code of ref document: T

Effective date: 20141115

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502006014018

Country of ref document: DE

Effective date: 20141127

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2525324

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20141222

REG Reference to a national code

Ref country code: DK

Ref legal event code: T3

Effective date: 20150115

REG Reference to a national code

Ref country code: SE

Ref legal event code: TRGR

REG Reference to a national code

Ref country code: NL

Ref legal event code: T3

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150215

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150216

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150116

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 502006014018

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20150716

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

Ref country code: LU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150422

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20160323

Year of fee payment: 11

Ref country code: BE

Payment date: 20160324

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20160411

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20160420

Year of fee payment: 11

Ref country code: DE

Payment date: 20160419

Year of fee payment: 11

Ref country code: IE

Payment date: 20160411

Year of fee payment: 11

Ref country code: ES

Payment date: 20160331

Year of fee payment: 11

Ref country code: CH

Payment date: 20160411

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DK

Payment date: 20160412

Year of fee payment: 11

Ref country code: AT

Payment date: 20160330

Year of fee payment: 11

Ref country code: SE

Payment date: 20160412

Year of fee payment: 11

Ref country code: IT

Payment date: 20160418

Year of fee payment: 11

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20060422

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141015

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 502006014018

Country of ref document: DE

REG Reference to a national code

Ref country code: DK

Ref legal event code: EBP

Effective date: 20170430

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

REG Reference to a national code

Ref country code: NL

Ref legal event code: MM

Effective date: 20170501

REG Reference to a national code

Ref country code: AT

Ref legal event code: MM01

Ref document number: 691904

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170422

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20170422

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

Effective date: 20171229

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171103

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170422

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170502

Ref country code: NL

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170501

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170423

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170430

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170430

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170422

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20170430

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170430

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170422

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170430

Ref country code: IT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170422

REG Reference to a national code

Ref country code: ES

Ref legal event code: FD2A

Effective date: 20180703

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170423