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EP1764154A1 - Device for comminuting biological samples - Google Patents

Device for comminuting biological samples Download PDF

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Publication number
EP1764154A1
EP1764154A1 EP05020207A EP05020207A EP1764154A1 EP 1764154 A1 EP1764154 A1 EP 1764154A1 EP 05020207 A EP05020207 A EP 05020207A EP 05020207 A EP05020207 A EP 05020207A EP 1764154 A1 EP1764154 A1 EP 1764154A1
Authority
EP
European Patent Office
Prior art keywords
shaft
coupling
sample container
coupling piece
working
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP05020207A
Other languages
German (de)
French (fr)
Other versions
EP1764154B1 (en
Inventor
Stefan Jenni
Daniel Krieg
Karsten Meissner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prionics AG
Original Assignee
Prionics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP05020207A priority Critical patent/EP1764154B1/en
Application filed by Prionics AG filed Critical Prionics AG
Priority to ES05020207T priority patent/ES2299933T3/en
Priority to AT05020207T priority patent/ATE385853T1/en
Priority to DE502005002844T priority patent/DE502005002844D1/en
Priority to PCT/EP2006/008329 priority patent/WO2007033748A1/en
Priority to CA2622138A priority patent/CA2622138C/en
Priority to US12/066,979 priority patent/US7980500B2/en
Priority to AU2006294111A priority patent/AU2006294111B2/en
Priority to CN2006800310196A priority patent/CN101247891B/en
Publication of EP1764154A1 publication Critical patent/EP1764154A1/en
Application granted granted Critical
Publication of EP1764154B1 publication Critical patent/EP1764154B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
    • B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
    • B02C18/10—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers with drive arranged above container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
    • B02C18/16—Details
    • B02C18/24—Drives

Definitions

  • the invention relates to a device according to the preamble of claim 1.
  • Generic devices are used in the investigation of particular solid biological sample material. It may be e.g. to body tissue samples but also other sample material of biological origin act, having solid components.
  • sample material is homogenized, for example in a buffer or the like.
  • a comminution of the sample material must be carried out prior to examination.
  • a generic, used in particular for crushing and homogenization of bovine brain tissue in the context of BSE tests device is, for example from the WO 02/48679 known.
  • the known device has a sample container with a shaft rotatably disposed therein.
  • the shaft is supported by a working end on the bottom of the sample container.
  • a cutting device with several laterally protruding blades is provided on the shaft, which comminute sample material located during rotation of the shaft in the sample container.
  • an externally accessible coupling piece is provided, which can be brought into clutch engagement with a coupling counterpart of a separate rotary drive device.
  • the rotary drive device is designed so that it can rotate the shaft with a relatively high number of revolutions (about 20,000 rpm).
  • a disadvantage of the known device is that despite the ball bearing in operation, an increased friction occurs in the region of the working end of the shaft, which can lead to difficulties.
  • the object of the invention is, starting from the prior art to provide a device in which the shaft can be rotated with the least possible friction.
  • the device according to the invention like the known device, has a sample container in which a shaft is rotatably and axially displaceably arranged.
  • the shaft has a working end that can contact the bottom of the sample container when the shaft is at rest.
  • a laterally projecting cutting device In the area of the working end, a laterally projecting cutting device is provided, which comminutes the sample material during rotation of the shaft.
  • the cutter may include one or more blades projecting laterally from the shaft. The blades can continue to be provided in different height ranges of the shaft.
  • the indication "area of the end of work” is relatively broad. Essentially, this means that the cutting device is arranged in a region of the shaft which dips into the sample material during normal workup.
  • the shaft further has a coupling end on which a coupling piece is provided, which can be brought into coupling engagement with the counterpart of a separate rotary drive device.
  • the term coupling piece or coupling counterpart is to be understood. It may be one or more formed on the respective components and coordinated surfaces and / or shapes, etc.
  • the shaft is guided laterally and axially displaceable in the sample container.
  • a lateral guide is provided in the region of the coupling end of the shaft.
  • An axial displacement of the shaft towards its coupling end may e.g. be limited by a formed on the shaft stop or the like.
  • a movement of the shaft in the opposite direction is z.b. bounded by the bottom of the sample container.
  • the coupling piece and the counterpart coupling piece are designed to produce a coupling engagement that is positive in the direction of rotation and permits a displacement of the shaft in the axial direction.
  • the invention provides that the laterally arranged on the shaft cutter is propeller-shaped such that it exerts a propulsive force on the shaft in the direction of the coupling end upon rotation of the shaft in the working direction.
  • the shaft and with it the cutting device are rotated in the working direction and begin to comminute the sample material.
  • a driving force acting on the shaft builds up depending on the surrounding medium from a certain number of revolutions the shaft axially in the direction of the rotary drive means, which is possible due to the inventive design of coupling piece and coupling counterpart. This increases the distance of the working end of the shaft to the bottom of the sample container, with the Consequence that the working end can rotate without contact to the ground, thus without friction.
  • the corresponding design of the cutting device is not a problem for the expert. It is sufficient to provide the blade or blades of the cutting device with a corresponding inclination or leading edges to produce the necessary buoyancy. It may be a laterally projecting cutting device with a blade or multiple blades that protrude laterally at a height or different heights of the shaft.
  • shaft rotates without axial support of its working end on the bottom of the sample container.
  • this can lead to the shaft swinging out laterally undesirably on contact of the cutting device with the sample material.
  • a preferred embodiment provides that a bush is provided on the bottom of the sample container for lateral guidance of the working end of the shaft.
  • the provided at the coupling end of the shaft coupling piece one or more laterally projecting in Has longitudinally extending projections.
  • two projections for example projecting on opposite sides of the shaft, are provided whose upper edges have different distances from the coupling end.
  • a tilting between the coupling piece and coupling counterpart can be particularly reliably avoided.
  • a seal of the opening can be achieved in a particularly simple manner by forming a shaft arranged on the circumferential projection.
  • the projection is dimensioned and positioned on the shaft so that it can rest against the passage opening in the direction of the coupling end during the axial displacement of the shaft during operation.
  • the projection thus also serves as a stop which limits an axial displacement of the shaft.
  • the figure shows a sample container 10, which has a lower cup-shaped region 11 with a bottom 12, and an upper part 13 which is placed on the cup-shaped part 11.
  • a shaft 14 is rotatably arranged and displaceable in the axial direction.
  • the shaft has a working end 15.
  • a cutting device 16 is provided, which has several laterally projecting blades 17 in the case shown.
  • the other end of the shaft 14 is formed as a coupling end 18 and has an externally accessible coupling piece, in the form of two laterally of the shaft 14 protruding in the longitudinal direction extending projections 19 and 190 is formed.
  • the shaft 14 With its coupling end 18, the shaft 14 can be brought into engagement with an only indicated separate rotary drive device 20 having a corresponding coupling counterpart.
  • the region of the coupling counterpart which engages with projection 19 is designated in the figure by 21.
  • the projections 19 and 190 extend differently far in the direction of the coupling end 18 of the shaft 14, whereby tilting in the coupling counterpart can be avoided.
  • the shaft 14 may experience a displacement in which the working end 15 moves away from the bottom 12 of the sample container 10.
  • the displacement is limited by a projection 24 formed circumferentially around the shaft, which serves as a stop and, in the stop position, at the same time seals a passage opening 30 provided in the upper part 13 of the sample container 10 for the shaft 14.
  • a projection 14 which encircles the shaft 14 is formed, with which the shaft 14 is guided laterally.
  • a bushing 25 is further provided on the bottom 12 of the sample container 10, which ensures a lateral guidance of the shaft 14 in this area.

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  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Disintegrating Or Milling (AREA)
  • Crushing And Pulverization Processes (AREA)

Abstract

The device has an axially displaceable spindle (14) with a coupling end (18), arranged in a sample container. An operating end of the spindle contacts with a base of the container in an idle condition of the spindle. A cutting mechanism (16) is formed in the shape of a propeller so that a propelling force is exerted on the propeller in the direction of the coupling end, during the rotation of the spindle in the operating direction.

Description

Die Erfindung bezieht sich auf eine Vorrichtung nach dem Oberbegriff des Anspruches 1.The invention relates to a device according to the preamble of claim 1.

Gattungsgemäße Vorrichtungen kommen zum Einsatz bei der Untersuchung von insbesondere festem biologischem Probematerial. Es kann sich dabei z.B. um Körpergewebeproben aber auch sonstiges Probematerial biologischen Ursprungs handeln, das feste Bestandteile aufweist.Generic devices are used in the investigation of particular solid biological sample material. It may be e.g. to body tissue samples but also other sample material of biological origin act, having solid components.

In aller Regel setzt eine Untersuchung voraus, daß das Probematerial homogenisiert, z.B. in einem Puffer oder dergleichen, vorliegt. Bei festem Probematerial, z.B. Körpergewebe, muß dazu vor Untersuchung eine Zerkleinerung des Probematerials vorgenommen werden.As a rule, an investigation requires that the sample material is homogenized, for example in a buffer or the like. For solid sample material, eg body tissue, a comminution of the sample material must be carried out prior to examination.

Eine gattungsgemäße, insbesondere zur Zerkleinerung und Homogenisierung von Rinderhirngewebe im Rahmen von BSE-Tests eingesetzte Vorrichtung, ist z.B. aus der WO 02/48679 bekannt.A generic, used in particular for crushing and homogenization of bovine brain tissue in the context of BSE tests device is, for example from the WO 02/48679 known.

Die bekannte Vorrichtung weist einen Probenbehälter auf mit einer darin drehbar angeordneten Welle. Die Welle stützt sich mit einem Arbeitsende auf dem Boden des Probenbehälters ab. Im Bereich ihre Arbeitsendes ist an der Welle eine Schneideinrichtung mit mehreren seitlich abstehenden Klingen vorgesehen, die bei Rotation der Welle im Probenbehälter befindliches Probematerial zerkleinern. An dem anderen Ende der Welle (Kupplungsende) ist ein von außen zugängliches Kupplungsstück vorgesehen, das in Kupplungseingriff mit einem Kupplungsgegenstück einer separaten Drehantriebseinrichtung gebracht werden kann. Die Drehantriebseinrichtung ist so ausgelegt, daß sie die Welle mit relativ hoher Umdrehungszahl (ca. 20.000 rpm) rotieren kann.The known device has a sample container with a shaft rotatably disposed therein. The shaft is supported by a working end on the bottom of the sample container. In the area of their working end, a cutting device with several laterally protruding blades is provided on the shaft, which comminute sample material located during rotation of the shaft in the sample container. At the other end of the shaft (coupling end) an externally accessible coupling piece is provided, which can be brought into clutch engagement with a coupling counterpart of a separate rotary drive device. The rotary drive device is designed so that it can rotate the shaft with a relatively high number of revolutions (about 20,000 rpm).

Bei der bekannten Konstruktion ist der Kupplungseingriff kraftschlüssig, mit anderen Worten die Drehantriebseinrichtung bzw. ihr Kupplungsgegenstück drückt von oben auf das Kupplungsende der Welle. Um das Risiko eventuellen Schlupfs gering zu halten bzw. auszuschließen, muss mit relativ hoher Anpresskraft gekuppelt werden, mit der Folge, dass das Arbeitsende der Welle ebenfalls mit hohem Druck auf dem Boden des Probenbehälters anliegt. Um die hier auftretende Reibung möglichst gering zu halten, ist gemäß der WO 02/48679 eine Lagerung mit einer Metallkugel zwischen Boden und Arbeitsende der Welle vorgesehen.In the known construction of the clutch engagement is non-positively, in other words, the rotary drive device or its coupling counterpart presses from above on the coupling end of the shaft. In order to minimize or exclude the risk of slippage, it is necessary to couple with a relatively high contact pressure, with the result that the working end of the shaft also bears against the bottom of the sample container at high pressure. In order to keep the friction occurring here as low as possible according to the WO 02/48679 a storage provided with a metal ball between the bottom and working end of the shaft.

Nachteilig an der bekannten Vorrichtung ist, daß trotz der Kugellagerung im Betrieb eine erhöhte Reibung im Bereich des Arbeitsendes der Welle auftritt, die zu Schwierigkeiten führen kann.A disadvantage of the known device is that despite the ball bearing in operation, an increased friction occurs in the region of the working end of the shaft, which can lead to difficulties.

Aufgabe der Erfindung ist es, ausgehend von dem Stand der Technik eine Vorrichtung zu schaffen, bei der die Welle mit möglichst wenig Reibung rotiert werden kann.The object of the invention is, starting from the prior art to provide a device in which the shaft can be rotated with the least possible friction.

Gelöst wird die Aufgabe mit einer Vorrichtung, die die kennzeichnenden Merkmale des Anspruches 1 aufweist.The problem is solved with a device having the characterizing features of claim 1.

Die erfindungsgemäße Vorrichtung weist wie die bekannte Vorrichtung einen Probenbehälter auf, in dem eine Welle drehbar und axial verschiebbar angeordnet ist. Die Welle weist ein Arbeitsende auf, das im Ruhezustand der Welle den Boden des Probenbehälters kontaktieren kann.The device according to the invention, like the known device, has a sample container in which a shaft is rotatably and axially displaceably arranged. The shaft has a working end that can contact the bottom of the sample container when the shaft is at rest.

Im Bereich des Arbeitsendes ist eine seitlich abstehende Schneideinrichtung vorgesehen, die bei Rotation der Welle das Probematerial zerkleinert. Die Schneideinrichtung kann eine oder mehrere seitlich von der Welle abstehende Klingen umfassen. Die Klingen können weiterhin auch in unterschiedlichen Höhenbereichen der Welle vorgesehen sein.In the area of the working end, a laterally projecting cutting device is provided, which comminutes the sample material during rotation of the shaft. The cutter may include one or more blades projecting laterally from the shaft. The blades can continue to be provided in different height ranges of the shaft.

Die Angabe "Bereich des Arbeitsendes" ist relativ weit zu fassen. Gemeint ist damit im wesentlichen, daß die Schneideinrichtung in einem Bereich der Welle angeordnet ist, der bei üblichen Aufarbeitungen in das Probematerial eintaucht.The indication "area of the end of work" is relatively broad. Essentially, this means that the cutting device is arranged in a region of the shaft which dips into the sample material during normal workup.

Die Welle weist weiterhin ein Kupplungsende auf, an dem ein Kupplungsstück vorgesehen ist, das in Kupplungseingriff mit dem Gegenstück einer separaten Drehantriebseinrichtung gebracht werden kann. Auch der Begriff Kupplungsstück bzw. Kupplungsgegenstück ist weit zu fassen. Es kann sich dabei um eine oder mehrere auf den jeweiligen Bauteilen ausgebildete und aufeinander abgestimmte Flächen und/oder Formen etc. handeln.The shaft further has a coupling end on which a coupling piece is provided, which can be brought into coupling engagement with the counterpart of a separate rotary drive device. The term coupling piece or coupling counterpart is to be understood. It may be one or more formed on the respective components and coordinated surfaces and / or shapes, etc.

Erfindungsgemäß ist vorgesehen, daß die Welle seitlich geführt und axial verschiebbar in dem Probenbehälter angeordnet ist. Im einfachsten Fall wird nur eine seitliche Führung im Bereich des Kupplungsendes der Welle vorgesehen. Eine axiale Verschiebung der Welle in Richtung auf ihr Kupplungsende kann z.B. durch einen auf der Welle ausgebildeten Anschlag oder dergleichen begrenzt werden. Eine Bewegung der Welle in Gegenrichtung wird z.b. durch den Boden des Probenbehälters begrenzt.According to the invention it is provided that the shaft is guided laterally and axially displaceable in the sample container. In the simplest case, only a lateral guide is provided in the region of the coupling end of the shaft. An axial displacement of the shaft towards its coupling end may e.g. be limited by a formed on the shaft stop or the like. A movement of the shaft in the opposite direction is z.b. bounded by the bottom of the sample container.

Als weiteres Merkmal der Erfindung ist vorgesehen, dass das Kupplungsstück und das Kupplungsgegenstück zur Erzeugung eines in Drehrichtung formschlüssigen, in Axialrichtung eine Verschiebung der Welle zulassenden Kupplungseingriffes ausgebildet sind.As a further feature of the invention, it is provided that the coupling piece and the counterpart coupling piece are designed to produce a coupling engagement that is positive in the direction of rotation and permits a displacement of the shaft in the axial direction.

Schließlich ist erfindungsgemäß vorgesehen, daß die seitlich an der Welle angeordnete Schneideinrichtung propellerförmig derart ausgebildet ist, daß sie bei Drehung der Welle in Arbeitsrichtung eine Vortriebskraft auf die Welle in Richtung des Kupplungsendes ausübt.Finally, the invention provides that the laterally arranged on the shaft cutter is propeller-shaped such that it exerts a propulsive force on the shaft in the direction of the coupling end upon rotation of the shaft in the working direction.

Die erfindungsgemäß in der Vorrichtung vorgesehenen Merkmale wirken wie folgt zusammen:The features provided according to the invention in the device interact as follows:

Nach Herstellung des Kupplungseingriffes mit der Drehantriebseinrichtung wird die Welle und mit ihr die Schneideinrichtung in Arbeitsrichtung rotiert und fängt an, das Probematerial zu zerkleinern. Gleichzeitig baut sich eine auf die Welle wirkende Vortriebskraft auf, die abhängig von dem umgebenden Medium ab einer bestimmten Umdrehungszahl die Welle axial in Richtung der Drehantriebseinrichtung verschiebt, was aufgrund der erfindungsgemäßen Ausbildung von Kupplungstück und Kupplungsgegenstück möglich ist. Dabei vergrößert sich der Abstand des Arbeitsendes der Welle zum Boden des Probenbehälters, mit der Folge dass das Arbeitsende ohne Kontakt zum Boden, also ohne Reibung, rotieren kann.After the coupling engagement with the rotary drive device has been established, the shaft and with it the cutting device are rotated in the working direction and begin to comminute the sample material. At the same time, a driving force acting on the shaft builds up depending on the surrounding medium from a certain number of revolutions the shaft axially in the direction of the rotary drive means, which is possible due to the inventive design of coupling piece and coupling counterpart. This increases the distance of the working end of the shaft to the bottom of the sample container, with the Consequence that the working end can rotate without contact to the ground, thus without friction.

Die entsprechende Ausbildung der Schneideinrichtung stellt für den Fachmann kein Problem dar. Es reicht aus, die Klinge bzw. Klingen der Schneideinrichtung mit einer entsprechenden Neigung bzw. Leitkanten zu versehen, um den nötigen Auftrieb zu erzeugen. Es kann sich dabei um eine seitlich abstehende Schneideinrichtung mit einer Klinge oder mehreren Klingen handeln, die seitlich auf einer Höhe oder auch unterschiedlichen Höhen von der Welle abstehen.The corresponding design of the cutting device is not a problem for the expert. It is sufficient to provide the blade or blades of the cutting device with a corresponding inclination or leading edges to produce the necessary buoyancy. It may be a laterally projecting cutting device with a blade or multiple blades that protrude laterally at a height or different heights of the shaft.

Durch Kombination eines Kupplungseingriffes zwischen Welle und Drehantriebseinrichtung, der eine axiale Verschiebung der Welle ermöglicht, sowie der bei Rotation der Welle durch die Schneideinrichtung erzeugten Vortriebswirkung wird in einfacher und sicherer Weise das Auftreten von Reibung zwischen dem Arbeitsende der Welle und dem Boden des Probenbehälters vermieden.By combining a coupling engagement between shaft and rotary drive means, which allows axial displacement of the shaft, as well as the driving action generated by rotation of the shaft by the cutter is avoided in a simple and secure manner, the occurrence of friction between the working end of the shaft and the bottom of the sample container.

Vorteilhafte Ausgestaltungen der Erfindung sind in den Unteransprüchen angegeben.Advantageous embodiments of the invention are specified in the subclaims.

Wie oben bereits ausgeführt, dreht sich Welle ohne axiale Abstützung ihres Arbeitsendes auf dem Boden des Probenbehälters. Insbesondere bei sehr festem Probematerial kann dies dazu führen, daß die Welle bei Kontakt der Schneideinrichtung mit dem Probematerial unerwünscht seitlich ausschwingt. Um dies zu vermeiden, sieht eine bevorzugte Ausgestaltung vor, daß auf dem Boden des Probenbehälters eine Buchse vorgesehen ist zur seitlichen Führung des Arbeitsendes der Welle.As stated above, shaft rotates without axial support of its working end on the bottom of the sample container. In particular, in the case of very firm sample material, this can lead to the shaft swinging out laterally undesirably on contact of the cutting device with the sample material. To avoid this, a preferred embodiment provides that a bush is provided on the bottom of the sample container for lateral guidance of the working end of the shaft.

In einer weiteren Ausgestaltung ist vorgesehen, daß das am Kupplungsende der Welle vorgesehene Kupplungsstück einen bzw. mehrere seitlich abstehende in Längsrichtung erstreckte Vorsprünge aufweist. Besonders bevorzugt werden zwei z.B auf gegenüberliegenden Seiten der Welle abstehende Vorsprünge vorgesehen, deren obere Kanten unterschiedliche Abstände zu dem Kupplungsende aufweisen. Bei dieser Ausgestaltung lässt sich besonders zuverlässig eine Verkantung zwischen Kupplungsstück und Kupplungsgegenstück vermeiden.In a further embodiment it is provided that the provided at the coupling end of the shaft coupling piece one or more laterally projecting in Has longitudinally extending projections. Particularly preferably, two projections, for example projecting on opposite sides of the shaft, are provided whose upper edges have different distances from the coupling end. In this embodiment, a tilting between the coupling piece and coupling counterpart can be particularly reliably avoided.

Ein weiteres Problem ist, daß im Betrieb Flüssigkeit in dem Probenbehälter aufsteigen und durch die für die Welle vorgesehene Durchlaßöffnung austreten kann. Eine Abdichtung der Öffnung lässt sich in besonders einfacher Weise erreichen durch Ausbildung eines an der Welle angeordneten umlaufenden Vorsprungs. Der Vorsprung ist so dimensioniert und an der Welle positioniert, dass er sich bei der im Betrieb eintretenden axialen Verschiebung der Welle in Richtung des Kupplungsendes an die Durchlassöffnung anlegen kann. Der Vorsprung dient damit gleichzeitig auch als Anschlag, der eine axiale Verschiebung der Welle begrenzt.Another problem is that, in operation, liquid may rise in the sample container and exit through the passageway provided for the shaft. A seal of the opening can be achieved in a particularly simple manner by forming a shaft arranged on the circumferential projection. The projection is dimensioned and positioned on the shaft so that it can rest against the passage opening in the direction of the coupling end during the axial displacement of the shaft during operation. The projection thus also serves as a stop which limits an axial displacement of the shaft.

Im Folgenden soll die Erfindung anhand einer Figur näher erläutert werden.In the following, the invention will be explained in more detail with reference to a figure.

Die Figur zeigt einen Probenbehälter 10, der einen unteren becherförmigen Bereich 11 mit einem Boden 12 aufweist, sowie ein auf den becherförmigen Teil 11 aufgesetztes Oberteil 13.The figure shows a sample container 10, which has a lower cup-shaped region 11 with a bottom 12, and an upper part 13 which is placed on the cup-shaped part 11.

In dem Probenbehälter 11 ist eine Welle 14 drehbar und in axialer Richtung verschiebbar angeordnet. Die Welle weist ein Arbeitsende 15 auf. Im Bereich des Arbeitsendes 15 ist eine Schneideinrichtung 16 vorgesehen, die im gezeigten Fall mehrere seitlich abstehende Klingen 17 aufweist.In the sample container 11, a shaft 14 is rotatably arranged and displaceable in the axial direction. The shaft has a working end 15. In the region of the working end 15, a cutting device 16 is provided, which has several laterally projecting blades 17 in the case shown.

Das andere Ende der Welle 14 ist als Kupplungsende 18 ausgebildet und weist ein von aussen zugängliches Kupplungsstück, das in Form von zwei seitlich von der Welle 14 abstehenden in Längsrichtung erstreckten Vorsprüngen 19 und 190 ausgebildet ist. Mit ihrem Kupplungsende 18 kann die Welle 14 in Eingriff mit einer nur angedeuteten separaten Drehantriebseinrichtung 20 gebracht werden, das ein entsprechendes Kupplungsgegenstück aufweist. Der mit Vorsprung 19 in Eingriff gelangende Bereich des Kupplungsgegenstücks ist in der Figur mit 21 bezeichnet ist.The other end of the shaft 14 is formed as a coupling end 18 and has an externally accessible coupling piece, in the form of two laterally of the shaft 14 protruding in the longitudinal direction extending projections 19 and 190 is formed. With its coupling end 18, the shaft 14 can be brought into engagement with an only indicated separate rotary drive device 20 having a corresponding coupling counterpart. The region of the coupling counterpart which engages with projection 19 is designated in the figure by 21.

Die Vorsprünge 19 und 190 erstrecken sich unterschiedlich weit in Richtung des Kupplungsendes 18 der Welle 14, wodurch Verkantungen im Kupplungsgegenstück vermieden werden.The projections 19 and 190 extend differently far in the direction of the coupling end 18 of the shaft 14, whereby tilting in the coupling counterpart can be avoided.

Wie die Figur schematisch zeigt, ist der zwischen Kupplungsstück 19 und Kupplungsstück 21 herstellbare Kupplungseingriff in Drehrichtung formschlüssig, und ermöglicht gleichzeitig eine Axialverschiebung der Welle 14 in Richtung des Kupplungsendes 18, wie durch den Pfeil 22 angedeutet. Eine Axialverschiebung der Welle 14 in Richtung des Pfeiles 22 tritt dann auf, wenn die Welle 14 in der durch Pfeil 23 angegebenen Arbeitsrichtung rotiert wird. Durch die gezeigte propellerförmige Anordnung der Klingen 17 entsteht eine Vortriebskraft auf die Welle 14, die in Richtung des Pfeiles 22 wirkt.As the figure shows schematically, the coupling engagement between coupling piece 19 and coupling piece 21 producible clutch engagement in the direction of rotation form-fitting manner, while allowing axial displacement of the shaft 14 in the direction of the coupling end 18, as indicated by the arrow 22. An axial displacement of the shaft 14 in the direction of the arrow 22 occurs when the shaft 14 is rotated in the direction indicated by arrow 23 working direction. Due to the propeller-shaped arrangement of the blades 17 shown, a driving force is produced on the shaft 14, which acts in the direction of the arrow 22.

Als Folge kann die Welle 14 eine Verschiebung erfahren, bei der sich das Arbeitsende 15 vom Boden 12 des Probenbehälters 10 entfernt. Begrenzt wird die Verschiebung durch einen die Welle umlaufend ausgebildeten Vorsprung 24, der als Anschlag dient und in Anschlagsposition gleichzeitig eine in dem Oberteil 13 des Probenbehälters 10 vorgesehene Durchlaßöffnung 30 für die Welle 14 abdichtet. Im Bereich der Durchlassöffnung 30 ist ein die Welle 14 umlaufender Vorsprung 36 ausgebildet, mit dem die Welle 14 seitlich geführt ist.As a result, the shaft 14 may experience a displacement in which the working end 15 moves away from the bottom 12 of the sample container 10. The displacement is limited by a projection 24 formed circumferentially around the shaft, which serves as a stop and, in the stop position, at the same time seals a passage opening 30 provided in the upper part 13 of the sample container 10 for the shaft 14. In the region of the passage opening 30, a projection 14 which encircles the shaft 14 is formed, with which the shaft 14 is guided laterally.

Um sicherzustellen, daß während der Rotation der Welle 14 kein unerwünschtes seitliches Ausschwingen des Arbeitsendes 15 erfolgt, ist weiterhin auf dem Boden 12 des Probenbehälters 10 eine Buchse 25 vorgesehen, die eine seitliche Führung der Welle 14 auch in diesem Bereich gewährleistet.In order to ensure that during the rotation of the shaft 14 no unwanted lateral swinging of the working end 15 takes place, a bushing 25 is further provided on the bottom 12 of the sample container 10, which ensures a lateral guidance of the shaft 14 in this area.

Claims (5)

Vorrichtung zum Zerkleinern von biologischem Probematerial, mit einem Probenbehälter, mit einer von dem Probenbehälter separaten Drehantriebseinrichtung, und mit einer im Probenbehälter drehbar angeordneten Welle, wobei die Welle im Bereich eines Arbeitsendes eine seitlich abstehende Schneideinrichtung aufweist, und im Bereich eines Kupplungsendes ein von aussen zugängliches Kupplungsstück aufweist, das zum Kupplungseingriff mit einem Kupplungsgegenstück der Drehantriebseinrichtung ausgebildet ist, dadurch gekennzeichnet, dass die Welle (14) mit seitlicher Führung und axial verschiebbar in dem Probenbehälter (10) angeordnet ist, das Kupplungsstück (19) und das Kupplungsgegenstück (21) zur Erzeugung eines in Drehrichtung (23) formschlüssigen, eine Axialverschiebung zulassenden Kupplungseingriffs ausgebildet sind, und die Schneideinrichtung (16) propellerförmig derart ausgebildet ist, dass sie bei Drehung der Welle (14) in Arbeitsrichtung auf diese eine Vortriebskraft in Richtung des Kupplungsendes (18) ausübt.Apparatus for shredding biological sample material, comprising a sample container, having a rotary drive device separate from the sample container, and having a shaft rotatably mounted in the sample container, the shaft having a laterally projecting cutter in the region of a working end and an externally accessible one in the region of a coupling end Coupling piece, which is designed for coupling engagement with a coupling counterpart of the rotary drive device, characterized in that the shaft (14) is arranged with lateral guidance and axially displaceable in the sample container (10), the coupling piece (19) and the coupling counterpart (21) Generation of a rotational direction (23) form-fitting, permitting an axial displacement clutch engagement are formed, and the cutting device (16) is propeller-shaped such that upon rotation of the shaft (14) in the working direction on this a driving force in Rich tion of the coupling end (18) exerts. Vorrichtung nach Anspruch 1 dadurch gekennzeichnet, dass auf dem Boden (12) des Probenbehälters (10) eine Buchse (25) zur seitlichen Führung des Arbeitsendes (15) der Welle (14) vorgesehen ist.Apparatus according to claim 1, characterized in that on the bottom (12) of the sample container (10) has a bush (25) for lateral guidance of the working end (15) of the shaft (14) is provided. Vorrichtung nach Anspruch 1, dadurch gekennzeichnet, dass das Kupplungsstück in Form von mindestens einem von der Welle (14) seitlich abstehenden und in Längsrichtung erstreckten Vorsprungs (19, 190) ausgebildet istApparatus according to claim 1, characterized in that the coupling piece in the form of at least one of the shaft (14) laterally projecting and in the longitudinal direction extending projection (19, 190) is formed Vorrichtung nach Anspruch 3, dadurch gekennzeichnet, dass das Kupplungsstück zwei seitlich abstehende Vorsprünge (19, 190) aufweist, deren obere Kanten unterschiedliche Abstände zu dem Kupplungsende aufweisen.Apparatus according to claim 3, characterized in that the coupling piece has two laterally protruding projections (19, 190) whose upper edges have different distances from the coupling end. Vorrichtung nach Anspruch 1, dadurch gekennzeichnet, dass an der Welle (14) ein umlaufender Vorsprung (24) vorgesehen ist, der so dimensioniert und angeordnet ist, dass er bei axialer Verschiebung der Welle in Richtung des Kupplungsendes (18), eine in dem Probenbehälter (10) vorgesehene Durchlassöffnung (30) für die Welle (14) abdeckt.Apparatus according to claim 1, characterized in that on the shaft (14) has a circumferential projection (24) which is dimensioned and arranged so that it in axial displacement of the shaft in the direction of the coupling end (18), one in the sample container (10) provided passage opening (30) for the shaft (14) covers.
EP05020207A 2005-09-16 2005-09-16 Device for comminuting biological samples Expired - Lifetime EP1764154B1 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
ES05020207T ES2299933T3 (en) 2005-09-16 2005-09-16 DEVICE FOR CRUSHING MATERIAL OF BIOLOGICAL SAMPLES.
AT05020207T ATE385853T1 (en) 2005-09-16 2005-09-16 DEVICE FOR GRINDING BIOLOGICAL SAMPLE MATERIAL
DE502005002844T DE502005002844D1 (en) 2005-09-16 2005-09-16 Apparatus for shredding biological sample material
EP05020207A EP1764154B1 (en) 2005-09-16 2005-09-16 Device for comminuting biological samples
PCT/EP2006/008329 WO2007033748A1 (en) 2005-09-16 2006-08-25 Apparatus for cutting up biological sample material
CA2622138A CA2622138C (en) 2005-09-16 2006-08-25 Apparatus for cutting up biological sample material
US12/066,979 US7980500B2 (en) 2005-09-16 2006-08-25 Apparatus for cutting up biological sample material
AU2006294111A AU2006294111B2 (en) 2005-09-16 2006-08-25 Apparatus for cutting up biological sample material
CN2006800310196A CN101247891B (en) 2005-09-16 2006-08-25 Equipment for pulverizing biological sample material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP05020207A EP1764154B1 (en) 2005-09-16 2005-09-16 Device for comminuting biological samples

Publications (2)

Publication Number Publication Date
EP1764154A1 true EP1764154A1 (en) 2007-03-21
EP1764154B1 EP1764154B1 (en) 2008-02-13

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EP05020207A Expired - Lifetime EP1764154B1 (en) 2005-09-16 2005-09-16 Device for comminuting biological samples

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US (1) US7980500B2 (en)
EP (1) EP1764154B1 (en)
CN (1) CN101247891B (en)
AT (1) ATE385853T1 (en)
AU (1) AU2006294111B2 (en)
CA (1) CA2622138C (en)
DE (1) DE502005002844D1 (en)
ES (1) ES2299933T3 (en)
WO (1) WO2007033748A1 (en)

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CN102438754A (en) * 2009-03-25 2012-05-02 福格申机械有限公司 Cutting device
US9700857B1 (en) 2012-03-23 2017-07-11 Life Technologies Corporation Fluid mixing system with drive shaft steady support
CN107185665A (en) * 2017-06-27 2017-09-22 北京东兴堂科技发展有限公司 Chinese herbal medicine crushes pelleting press

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CN105618237B (en) * 2016-02-29 2019-03-22 常州坚鹏建材有限公司 Crusher members abrasion resistant means suitable for hot mine process equipment
CN107700628A (en) * 2017-06-14 2018-02-16 广西南宁尼科安全技术服务有限公司 A kind of efficient water conservancy equipment
DK180133B1 (en) 2018-11-07 2020-06-09 Atline Aps Device for automated and contamination-free sampling and analysis
US12247902B2 (en) 2019-05-06 2025-03-11 Tissuemill Technologies Llc Atraumatically formed tissue compositions, devices and methods of preparation and treatment
US11033295B2 (en) 2019-05-06 2021-06-15 Tissuemill Technologies Llc Atraumatically formed tissue composition, devices and methods of preparation and treatment
US20210260593A1 (en) * 2020-02-24 2021-08-26 Kims Industry Heated herbal grinder and method therefor

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Also Published As

Publication number Publication date
DE502005002844D1 (en) 2008-03-27
CA2622138C (en) 2013-12-17
CN101247891A (en) 2008-08-20
ATE385853T1 (en) 2008-03-15
ES2299933T3 (en) 2008-06-01
US7980500B2 (en) 2011-07-19
WO2007033748A1 (en) 2007-03-29
EP1764154B1 (en) 2008-02-13
CA2622138A1 (en) 2007-03-29
US20080208234A1 (en) 2008-08-28
CN101247891B (en) 2010-05-19
AU2006294111A1 (en) 2007-03-29
AU2006294111B2 (en) 2010-08-05

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