EP1403641A1 - Méthode pour calculer des constants d'association et de dissociation en utilisant une puce de polymères pour l'identification des polymères ioniques - Google Patents
Méthode pour calculer des constants d'association et de dissociation en utilisant une puce de polymères pour l'identification des polymères ioniques Download PDFInfo
- Publication number
- EP1403641A1 EP1403641A1 EP20030019932 EP03019932A EP1403641A1 EP 1403641 A1 EP1403641 A1 EP 1403641A1 EP 20030019932 EP20030019932 EP 20030019932 EP 03019932 A EP03019932 A EP 03019932A EP 1403641 A1 EP1403641 A1 EP 1403641A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- ionic
- association
- dna
- probe
- polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
Definitions
- the invention provides a method of calculating an association constant and a dissociation constant comprising:
- a synthesized oligo DNA is spotted on a substrate, and a complementary oligo DNA with a labeled terminal is hybridized, thus manufacturing a double-stranded DNA chip.
- a substance of which the association constant is to be measured is added onto the double-stranded DNA chip as a sample.
- the complementary DNA is dissociated if the sample has not bound to the double-stranded DNA.
- it can be determined to what degree the sample to be measured has bound to the double-stranded DNA.
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002281530 | 2002-09-26 | ||
| JP2002281530A JP2004117201A (ja) | 2002-09-26 | 2002-09-26 | イオン性高分子同定用高分子チップを用いた結合定数および解離定数算出方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP1403641A1 true EP1403641A1 (fr) | 2004-03-31 |
| EP1403641B1 EP1403641B1 (fr) | 2006-03-29 |
Family
ID=31973313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP20030019932 Expired - Lifetime EP1403641B1 (fr) | 2002-09-26 | 2003-09-02 | Méthode pour calculer des constantes d'association et de dissociation en utilisant une puce de polymères pour l'identification des polymères ioniques |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1403641B1 (fr) |
| JP (1) | JP2004117201A (fr) |
| DE (1) | DE60304262T8 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1688179A3 (fr) * | 2005-02-02 | 2007-01-24 | Seiko Instruments Inc. | Microréacteur, appareil, procédé et programme de mesure de la constante de dissociation |
| CN101419214B (zh) * | 2007-10-23 | 2012-07-04 | 中国科学院上海药物研究所 | 基于分层原子加和模型的分子酸碱解离常数的预测方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002018648A2 (fr) * | 2000-08-25 | 2002-03-07 | President And Fellows Of Harvard College | Analyse d'interactions de liaison |
| US6355428B1 (en) * | 1998-09-11 | 2002-03-12 | Genelabs Technologies, Inc. | Nucleic acid ligand interaction assays |
| EP1348767A2 (fr) * | 2002-03-28 | 2003-10-01 | Hitachi Software Engineering Co., Ltd. | Puce de polymères et procédé d'identification d'un polymère ionique |
-
2002
- 2002-09-26 JP JP2002281530A patent/JP2004117201A/ja active Pending
-
2003
- 2003-09-02 DE DE2003604262 patent/DE60304262T8/de active Active
- 2003-09-02 EP EP20030019932 patent/EP1403641B1/fr not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6355428B1 (en) * | 1998-09-11 | 2002-03-12 | Genelabs Technologies, Inc. | Nucleic acid ligand interaction assays |
| WO2002018648A2 (fr) * | 2000-08-25 | 2002-03-07 | President And Fellows Of Harvard College | Analyse d'interactions de liaison |
| EP1348767A2 (fr) * | 2002-03-28 | 2003-10-01 | Hitachi Software Engineering Co., Ltd. | Puce de polymères et procédé d'identification d'un polymère ionique |
Non-Patent Citations (1)
| Title |
|---|
| MYSZKA DAVID G ET AL: "Equilibrium analysis of high affinity interactions using BIACORE", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 265, no. 2, 15 December 1998 (1998-12-15), pages 326 - 330, XP002201263, ISSN: 0003-2697 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1688179A3 (fr) * | 2005-02-02 | 2007-01-24 | Seiko Instruments Inc. | Microréacteur, appareil, procédé et programme de mesure de la constante de dissociation |
| CN101419214B (zh) * | 2007-10-23 | 2012-07-04 | 中国科学院上海药物研究所 | 基于分层原子加和模型的分子酸碱解离常数的预测方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE60304262T8 (de) | 2007-05-16 |
| EP1403641B1 (fr) | 2006-03-29 |
| DE60304262T2 (de) | 2006-12-28 |
| JP2004117201A (ja) | 2004-04-15 |
| DE60304262D1 (de) | 2006-05-18 |
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