[go: up one dir, main page]

EP1381467A2 - Procede et dispositif de manipulation de petites quantites de liquide sur des surfaces - Google Patents

Procede et dispositif de manipulation de petites quantites de liquide sur des surfaces

Info

Publication number
EP1381467A2
EP1381467A2 EP02732662A EP02732662A EP1381467A2 EP 1381467 A2 EP1381467 A2 EP 1381467A2 EP 02732662 A EP02732662 A EP 02732662A EP 02732662 A EP02732662 A EP 02732662A EP 1381467 A2 EP1381467 A2 EP 1381467A2
Authority
EP
European Patent Office
Prior art keywords
liquid
intermediate region
guide strip
guide
guide strips
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02732662A
Other languages
German (de)
English (en)
Inventor
Achim Wixforth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advalytix AG
Original Assignee
Advalytix AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advalytix AG filed Critical Advalytix AG
Publication of EP1381467A2 publication Critical patent/EP1381467A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/023—Sending and receiving of information, e.g. using bluetooth
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0493—Specific techniques used
    • B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/088—Passive control of flow resistance by specific surface properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24479—Structurally defined web or sheet [e.g., overall dimension, etc.] including variation in thickness
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/31504—Composite [nonstructural laminate]

Definitions

  • the invention relates to methods and devices for manipulating small amounts of liquid on surfaces, preferably chip surfaces.
  • liquid in the present text includes, among other things, pure liquids, mixtures, dispersions and suspensions, as well as liquids in which solid particles, e.g. B. biological material.
  • microstructured channels e.g. O. Müller, Laborwelt 1/2000, pages 36-38.
  • Such channels are e.g. B. etched in the chip and are several microns deep or wide and generally capped.
  • the movement is carried out by electrokinetic (M. Köhler et al, Physikalischet 56, No. 11, pages 57-61), mechanical or electrical pumps or capillary forces, each in microstructured channels.
  • a liquid or material contained therein is to be examined on a certain surface, a chemically, physically and / or biologically functionalized surface is often used.
  • the functionalized area In order to be able to carry out such an analysis or synthesis at a well-located location, the functionalized area must be located within a channel and is therefore difficult to manufacture. With a corresponding functionalization on a free surface without channel formation, on the other hand, an exact localization of the liquid cannot be guaranteed during the analysis.
  • the object of the present invention is to provide a method and a device with the aid of which a manipulation of a small amount of liquid along precisely defined transport routes or at defined analysis or synthesis points is possible, the method or the device being carried out or being economical and simple can be manufactured.
  • This object is achieved with a method with the features of claim 1, a method with the features of claim 3, a device with the features of claim 19 and a device with the features of claim 21.
  • the respective subclaims relate to preferred refinements or embodiments.
  • a small amount of liquid which is held together by its surface tension, is applied to an area of a surface, e.g. B. a solid surface, which includes an intermediate region and at least one guide strip.
  • the intermediate area is delimited by guide strips at least in one spatial direction.
  • the surface properties of the at least one intermediate area and the guide strips are selected so that a surface with a surface texture that corresponds to the surface texture of a guide strip wets more with the amount of liquid than a surface with the surface properties of the intermediate area. A flatter wetting angle will thus result between the liquid and a guide strip than between the liquid and the intermediate area.
  • the liquid is applied to the surface in such a way that it contacts both at least one guide strip and the intermediate area. The amount required for this can e.g. B. determined in preliminary tests or achieved by successive application of the liquid.
  • At least one correspondingly arranged guide strip is provided for delimiting an intermediate area with the corresponding surface properties.
  • the amount of liquid is held by the surface tension and by the preferred wetting with the surface of at least one guide strip.
  • the surface of the surface outer region which is adjacent to a guide strip on its side facing away from the adjacent intermediate region, can be such from the surface of one Intermediate areas have different wetting properties that the small amount of liquid wets more with the surface of the intermediate area.
  • the surface of the at least one guide strip is therefore most wetted by the liquid. Since the surface of the outer surface area is even less wetted by the amount of liquid than the intermediate area, the amount of liquid is thereby additionally localized in the area of the intermediate area and the guide strips.
  • the different surface textures can be achieved by appropriate coatings.
  • So z. B. for the manipulation of an aqueous solution the surface of the guide strips are selected to be hydrophilic in comparison to the surface of the intermediate region. In the case of oily solutions to be examined, the guide strips are chosen to be lipophilic in comparison to the intermediate area.
  • the coatings can in a simple manner, for. B. can be achieved by lithographic processes with subsequent coating steps. Different wetting properties can still be achieved by microstructuring, as is the case with the so-called lotus effect, which is based on different roughnesses of the surfaces. This can e.g. B. can be obtained by microstructure of the corresponding surface areas, for. B. chemical treatment or ion irradiation.
  • the intermediate area is raised relative to the at least one guide strip, the resulting step being smaller than the height of the amount of liquid held together by the surface tension on the intermediate area.
  • a gradation between the guide strip and the intermediate area can, for. B. by a very flat etching z. B. can be achieved with a depth in the submicron range of the surface of a solid state chip.
  • the intermediate region can consist of a coating using lithographic processes, or it can be in all of them Areas of the surface with the exception of the lead strips are grown in crystal material.
  • Macroscopic i.e. H. the surface remains essentially planar on a length scale in the order of magnitude of the width of the intermediate region or the lateral extent of the small amount of liquid.
  • Such a flat etching or step is very simple to manufacture and can be produced in a defined manner without the known problems of deep etching of narrow channels.
  • the configurations according to the invention have in common that there are guide strips at the edge of the intermediate region, to which the liquid would like to spread. In one embodiment, this is brought about by the preferred wetting of the guide strips, in the other embodiment by the downward leading step. On the sides of the intermediate area, the amount of liquid is guided or held by guide strips. The surface tension of the small amount of liquid also prevents it from running apart.
  • the amount of liquid can be in the form of a droplet on the intermediate area delimited by guide strips.
  • the liquid can also be in the form of a “tube” on the intermediate area with the adjacent guide strips.
  • the refinements according to the invention make it possible for a functionalization to be selected in the intermediate region independently of the wetting properties.
  • the guide strips ensure that the amount of liquid is located or guided. So it is z. B. possible that material in an aqueous solution is examined on a functionalized area that is hydrophobic.
  • the functionalized hydrophobic area represents the intermediate area, which is surrounded by hydrophilic or lowered guide strips. The liquid is thus kept in the functionalized area without deep etching being necessary to absorb the amount of liquid, although the functionalized area possibly hydrophobic.
  • the guide strips prevent the liquid from leaving the functionalized and possibly hydrophobic area.
  • a combination of the configurations with modulated wetting properties and lowered guide strips is also possible.
  • the width of the guide strips is chosen to be larger than the width of a precursor film of the liquid to be examined, preferably more than about 100 nanometers.
  • the precursor film is formed by condensation of liquid vapor on a solid (AW Adamson and AP Gast, "Physical Chemistry of Surfaces", John Wiley & Sons, Inc., New York 1997, 6th edition, pages 372, 373) in the environment a quantity of liquid on a surface regardless of the wetting angle.
  • the small amount of liquid is limited on several sides by guide strips. So an exact localization of the amount of liquid can be achieved to z. B. to be able to carry out a reaction at a localized point.
  • the liquid can e.g. B. on one of two sides limited by guide strips intermediate area in a type of guide rail.
  • an external force z For example, a drop of liquid is guided along such an intermediate area with laterally arranged guide strips as on a "track".
  • Different functionalized surface areas can be arranged in the intermediate area, with no particular consideration having to be given to their wetting properties.
  • only a slight braking force is exerted on the liquid by the surface, so that rapid transport is possible.
  • a plurality of guide strips are arranged parallel to one another, with an intermediate region being located between two guide strips.
  • the outer guide strips serve to limit the lateral movement of the liquid, while the guide strips located between them ensure the stabilization of the movement.
  • the methods according to the invention and the devices according to the invention are suitable for chemical, physical and / or biological analysis of the amount of liquid or of matter in the amount of liquid.
  • the intermediate area can be functionalized accordingly.
  • the method or the device can be used particularly advantageously to remove biological material, e.g. B. to examine cells or DNA molecules in buffer solutions.
  • the intermediate area is functionalized with the help of biological macromolecules.
  • the liquid is brought into the functionalized area and localized by the guide strips or limited in its movement in one spatial direction.
  • the biological material in the liquid may react with the biological macromolecules in the intermediate area. Resulting changes in the physical, chemical and / or biological behavior can be examined and used for analysis.
  • reaction areas can be provided on a chip, which, for example, enable “DNA screening”.
  • an external force is used to mix the small amount of liquid.
  • z. B. accelerate a reaction or achieve uniform reaction conditions.
  • the shape of the small amount of liquid changes in accordance with the lateral extent of the intermediate area with adjacent guide strips.
  • the external force can be in various ways, e.g. B. electrostatically generated.
  • generating an external force with the aid of surface acoustic waves is particularly advantageous.
  • Such surface sound waves lead to a mechanical deformation of the surface, which causes a momentum transfer to the amount of liquid.
  • Is z. B a piezoelectric crystal is used as a solid on which the arrangement is located, the mechanical deformation of the surface by the surface sound wave is additionally accompanied by electrical fields, which in turn cause a force effect on charged or polarizable matter within the liquid to be examined.
  • a surface sound wave transmits an impulse to the liquid.
  • the pulse causes the liquid to move in the direction of the propagation of the surface sound wave. Mixing of the liquid is also achieved by the action of the surface sound wave.
  • an interdigital transducer known per se is advantageously used on a piezoelectric area of the substrate or on a piezoelectric substrate. It is sufficient if the substrate or the corresponding coating is only piezoelectric in the area in which the interdigital transducer is located.
  • such an interdigital transducer has two electrodes which interlock with one another like fingers.
  • a high-frequency alternating field e.g. B. in the order of a few 10 to 100 MHz
  • a surface acoustic wave is excited in the piezoelectric substrate or in the piezoelectric region of the substrate when the resonance condition is almost fulfilled that the finger spacing of an electrode corresponds to the quotient of the surface sound velocity and the frequency.
  • the surface acoustic wave has the wavelength of the finger spacing of an electrode and its direction of propagation is essentially perpendicular to the intermeshing finger electrode structures.
  • Interdigital transducers can also, e.g. B. can be controlled wirelessly by irradiation of an alternating electromagnetic field in an antenna device connected to the interdigital transducer.
  • an interdigital transducer is arranged on the chip surface in such a way that one of its directions of sound propagation is essentially along the elongated arrangement of the intermediate area and guide strips.
  • interdigital transducers can be used to control various tracks formed by intermediate areas with adjoining guide strips.
  • a network arrangement of corresponding tracks and associated interdigital transducers is also possible.
  • interdigital transducers with a non-constant finger distance (“tapped interdigital transducer”), the lateral propagation area of the surface sound of an interdigital transducer can also be limited. With such a tapered interdigital transducer, different areas of a chip can be selected.
  • Interdigital transducers can be implemented in different geometries. According to the invention, other interdigital transducer geometries can also be used, as are known from the technology of surface acoustic wave filters.
  • the strength of the force effect on the small amount of liquid can be adjusted in a wide range via the amplitude or the pulse frequency of the surface sound wave.
  • the sonication of the solid surface with the surface sound wave can cause an automatic cleaning of the swept areas.
  • the method according to the invention and the device according to the invention can advantageously also be used in a system composed of different analysis or synthesis points on a solid-state chip.
  • a so-called “lab-on-a-chip” is formed.
  • Elongated intermediate areas with guide strips on both sides, if necessary, can serve as connecting paths between different analysis or synthesis points. which are surrounded on several sides by guide strips can be used as reaction areas.
  • the device according to the invention and the methods according to the invention can also be combined with other transport or localization methods on a chip.
  • the liquid is e.g. B. brought with the help of a pipetting robot so that at least one guide strip is touched.
  • the necessary amount can be determined in preliminary tests or can be achieved by successively applying the liquid.
  • the z. B. are arranged in parallel, the amount of liquid is localized by the preferred wetting of the guide strips in cooperation with the surface tension.
  • the liquid can also be applied in a self-adjusting manner.
  • the amount of liquid is brought into contact with a guide strip and the intermediate area.
  • external force e.g. B. by a surface sound wave or by moving the entire chip, the amount of liquid is moved so that it can come into contact with another guide strip.
  • a state then arises in a self-adjusting manner in which the amount of liquid is kept between them due to its surface tension and the preferred wetting of the guide strips.
  • Figure 1 is a plan view of an inventive device during the
  • Figure 2a shows a cross section through the arrangement of Figure 1 along the
  • Figure 2b shows a cross section along the line along the line BB of the figure
  • FIG. 2c shows a cross section along the line C-C with the viewing direction indicated in FIG. 2a
  • FIG. 3 shows an alternative embodiment in cross section
  • FIG. 4 shows a plan view of a further embodiment of the device according to the invention when carrying out a method according to the invention
  • Figure 5 shows a further embodiment of the device according to the invention when carrying out a method according to the invention.
  • Figure 1 shows a schematic representation and a top view of the implementation of a method according to the invention with a device according to the invention.
  • a section of a chip surface is shown.
  • the embodiment shown serves to transport a small amount of liquid 1 along a defined route.
  • 5 denotes lateral guide strips of width 8.
  • 1 denotes a drop of liquid which is held together on the solid surface by its own surface tension.
  • the guide strips are approximately one tenth to one third of the lateral extent of the amount of liquid to be manipulated, in the example shown the drop diameter, but larger than the width of the precursor film, ie larger than approximately 100 nm.
  • the liquid volumes range from 1 ⁇ m 3 to 1 cm 3 .
  • an intermediate area 3 which, for. B. is reaction functionalized.
  • biological macromolecules can be bound in the reaction-functionalized intermediate region 3.
  • the strip formed from the intermediate region 3 with the guide strips 5 is also not shown completely in FIG. 1, which is to be indicated by the lateral break lines 10. Different geometries and sizes are conceivable.
  • the interdigital transducer consists of electrodes 9 with finger-like projections 11 which interlock. The distance between the individual fingers is of the order of micrometers.
  • the arrangement is on a piezoelectric crystal, for. B. Lithium niobate.
  • the surface of the chip can be coated with a piezoelectric layer, e.g. B. made of zinc oxide.
  • the distance of the interdigital transducer 7 from the strip arrangement shown is not to scale. Larger distances are conceivable, as are known from surface acoustic wave technology.
  • Figure 2a shows a section through the arrangement of Figure 1 along the line AA in the viewing direction indicated in Figure 1.
  • the piezoelectric solid is denoted by 2.
  • a hydrophobic reaction functionalization 3 is located between the guide strips 5.
  • the surface wetting properties are chosen in such a way that there is less wetting than with the reaction-functionalized surface in the intermediate area 3. In this way, an additional localization of the amount of liquid on the intermediate area 3 and the guide strips 5 is achieved.
  • Figure 2b shows a section along the line B-B of Figure 2a. In this area, the liquid of the liquid drop 1 is located on the hydrophilic area 5 over the entire cross section.
  • the wetting angle a is set depending on the choice of materials.
  • Figure 2c shows a section along the line C-C of Figure 2a. Here the edge of the liquid is on the possibly hydrophobic part 3. Accordingly, the wetting angle ⁇ is much steeper than the wetting angle ⁇ of the liquid on the hydrophilic area in FIG. 2b.
  • Such an arrangement can be used as follows.
  • a drop of liquid 1 is brought onto the strip arrangement from the intermediate area 3 with the lateral guide strips 5.
  • the liquid drop 1 comprises an aqueous solution with z. B. biological material.
  • the necessary amount of liquid can be determined in preliminary tests.
  • z. B. a pipette can be successively increased until both guide strips 5 are at least touched. Due to the hydrophilic properties of the lateral guide strips 5, the liquid drop spreads completely in the direction of the edge. Due to the surface tension of the drop, it is held together in its shape and it does not run apart. This is additionally reinforced by the surface quality of the surface area 31, in which wetting takes place even less than in the intermediate area 3. In the intermediate area 3, which may have more hydrophobic properties than the guide strips 5, there is a steep wetting angle ⁇ , while in the area of the guide strips 5 there is a flatter wetting angle ⁇ .
  • An alternating electrical field of the specified magnitude is applied to the electrodes 9 of the interdigital transducer in order to generate a surface sound wave towards 13.
  • the surface sound wave transmits its momentum to the liquid drop 1, e.g. B. by the mechanical deformation of the surface.
  • the liquid drop is moved in this way in the direction 13.
  • the guide strips 5 prevent lateral breaking out. This behavior is independent of the wetting properties of the intermediate area 3.
  • a less hydrophilic region, as is present in the embodiment shown, is also possible for the intermediate region 3, since the direction of the drop movement is determined by the guide strips 5.
  • a defined movement of the drop of liquid along such a "track" is generated.
  • Such an arrangement can be used to the liquid drop z. B. to bring to a certain analysis point, in which the intermediate area 3 is functionalized in a special form to z. B. enable a reaction or analysis. No consideration needs to be given to the wetting properties of the intermediate area, since the movement of the drop is defined by the guide strips 5.
  • the liquid is arranged in the form of a droplet 1 on the surface.
  • the liquid is in the form of a “liquid hose” on the “track” 3, 5.
  • FIG. 3 An alternative embodiment is shown in FIG. The view corresponds to the illustration in FIG. 2a of the first embodiment.
  • the guide strips are achieved by flat depressions 50 in the surface.
  • there is a coating between the guide strips possibly with the desired reaction functionalization.
  • the thickness of the coating is thinner than about one tenth of the surface sound wavelength that can be generated with a transducer 11.
  • Outside the guide strips 50 there is a coating 30 with similar wetting properties as the reaction functionalization coating 3.
  • the depth 51 of the lowered areas 50 is very much smaller than the height 10 of the liquid drop 1, for. B. in the submicron range. A drop of liquid 1, which is brought onto the intermediate area 3, flows laterally into the guide strips 50.
  • the droplet of liquid 1 is also driven with an interdigital transducer in an arrangement similar to that in FIG. 1.
  • FIG. 5 An embodiment with a plurality of guide strips 5, 6 is shown in FIG. The same elements are again provided with the same reference numbers. While the outer guide strips 5 prevent the amount of liquid 1 from breaking out laterally, the guide strips 6 serve to further stabilize the movement in the direction 13.
  • the surface properties of the guide strips 6 correspond to the surface properties of the guide strips 5.
  • the surface areas are analogous to the embodiment of FIGS 5 and 6 formed hydrophilic when the liquid drop to be moved is an aqueous solution.
  • the wetting properties of the intermediate regions 3 can be chosen freely, so that a reaction functionalization can be provided which is independent of the wetting properties.
  • Figure 5 shows an arrangement such as. B. can be used for analysis.
  • the guide strips 5 form a border.
  • a reaction-functionalized region 4 is located within this border 5.
  • biological macromolecules can be bound to the surface.
  • the wetting properties of the surface area 4 can be selected independently of the type of the liquid 1, since a lateral breakout by the hydrophilic strips 5 in cooperation with the surface tension of the amount of liquid 1 is prevented.
  • an analysis can be carried out as follows. Via the intermediate regions 3 with the lateral guide strips 5, a quantity of liquid can be brought in the direction of the reaction region 4 with the aid of a surface acoustic wave which is generated with the interdigital transducer 7, as is the case, for example, with B. is described with reference to Figure 1.
  • the liquid 1 can be in the reaction area 4.
  • biological functionalization finds a reaction between the biological functionalization of the surface 4 with biological material, which, for. B. is contained in the liquid 1 instead.
  • the liquid can e.g. B. can be removed by further irradiation of a surface acoustic wave on the other side of the reaction region 4.
  • other, e.g. B. physical examinations are possible.
  • reaction areas 4 of this type can be connected to one another via corresponding “tracks” and thus enable different reactions with a liquid.
  • Other analysis or synthesis stations can also be provided, each of which is connected to one another via arrangements according to FIGS. 1 to 4 implement a "lab-on-the-chip" in which a very small amount of liquid is subjected to various tests.
  • the invention thus enables a defined movement of a quantity of liquid on a chip surface. No deeply etched channels with the known difficulties arising are necessary for this.
  • the surface remains essentially planar, can be cleaned very easily in this way and does not represent any additional obstacles to the movement of the amount of liquid. Due to the action of the surface sound wave, the amount of liquid is additionally turbulent and mixed. A reaction can be accelerated in this way.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Coating Apparatus (AREA)
  • Absorbent Articles And Supports Therefor (AREA)

Abstract

Procédés et dispositifs de manipulation de petites quantités de liquide sur des surfaces, de préférence sur des surfaces de puces. Selon la présente invention, une masse de liquide maintenue compacte par sa tension de surface est déposée sur une zone d'une surface dotée d'au moins une zone intermédiaire qui est adjacente à une bande de guidage dans au moins un direction latérale dans l'espace. Selon la présente invention, les bandes de guidage ont par rapport à la zone intermédiaire une qualité de surface tellement différente que lesdites bandes sont plus fortement mouillées par un liquide, ou alors la zone intermédiaire se trouve en relief par rapport aux bandes de guidage, la hauteur de l'épaulement étant faible par rapport à celle de la masse de liquide.
EP02732662A 2001-04-24 2002-04-24 Procede et dispositif de manipulation de petites quantites de liquide sur des surfaces Withdrawn EP1381467A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10120035 2001-04-24
DE10120035A DE10120035B4 (de) 2001-04-24 2001-04-24 Verfahren und Vorrichtung zur Manipulation kleiner Flüssigkeitsmengen auf Oberflächen
PCT/EP2002/004545 WO2002085520A2 (fr) 2001-04-24 2002-04-24 Procede et dispositif de manipulation de petites quantites de liquide sur des surfaces

Publications (1)

Publication Number Publication Date
EP1381467A2 true EP1381467A2 (fr) 2004-01-21

Family

ID=7682517

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02732662A Withdrawn EP1381467A2 (fr) 2001-04-24 2002-04-24 Procede et dispositif de manipulation de petites quantites de liquide sur des surfaces

Country Status (4)

Country Link
US (1) US7198813B2 (fr)
EP (1) EP1381467A2 (fr)
DE (1) DE10120035B4 (fr)
WO (1) WO2002085520A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10117772C2 (de) * 2001-04-09 2003-04-03 Advalytix Ag Mischvorrichtung und Mischverfahren für die Durchmischung kleiner Flüssigkeitsmengen
DE10164357B4 (de) * 2001-12-28 2005-11-10 Advalytix Ag Titrationsverfahren
EP1628905A1 (fr) * 2003-05-23 2006-03-01 Gyros Patent Ab Surfaces hydrophiles/hydrophobes
DE10326607A1 (de) * 2003-06-13 2005-01-05 Steag Microparts Gmbh Vorrichtung zum Handhaben von Flüssigkeiten
DE102004037348A1 (de) * 2004-08-02 2006-03-16 Infineon Technologies Ag Fluid-Transport-Vorrichtung, Sensor-Anordnung, Fluid-Misch-Vorrichtung und Verfahren zum Herstellen einer Fluid-Transport-Vorrichtung
US20070065637A1 (en) * 2005-09-16 2007-03-22 Extrand Charles W Carrier with anisotropic wetting surfaces
US20070062594A1 (en) * 2005-09-16 2007-03-22 Extrand Charles W Microfluidic device with anisotropic wetting surfaces
US20070065702A1 (en) * 2005-09-16 2007-03-22 Extrand Charles W Fuel cell with anisotropic wetting surfaces
WO2007066478A1 (fr) * 2005-12-08 2007-06-14 Olympus Corporation Reacteur et analyseur
JP2007232522A (ja) * 2006-02-28 2007-09-13 Olympus Corp 攪拌装置と分析装置
US8277761B2 (en) * 2006-08-03 2012-10-02 Agilent Technologies, Inc. Channelless fluidic sample transport medium
DE102007013688B3 (de) 2007-03-22 2008-11-06 Odelo Gmbh Optoelektronische Sensoreinrichtung und Verfahren zur Bestimmung der Transparenz einer Verschmutzungen ausgesetzten Oberfläche
FR2950544B1 (fr) 2009-09-29 2011-12-09 Ecole Polytech Circuit microfluidique
US10052431B2 (en) 2014-06-09 2018-08-21 Ascent Bio-Nano Technologies, Inc. System for manipulation and sorting of particles
US9645385B2 (en) * 2014-06-26 2017-05-09 Amazon Technologies, Inc. Methods and apparatuses for fabricating electrowetting displays

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001094017A1 (fr) * 2000-06-09 2001-12-13 Advalytix Ag Dispositif et procede permettant de manipuler de petites quantites de matiere
WO2002057014A1 (fr) * 2000-12-14 2002-07-25 Advalytix Ag Procede et dispositif de manipulation de petites quantites de liquides

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4233029A (en) 1978-10-25 1980-11-11 Eastman Kodak Company Liquid transport device and method
US4896174A (en) 1989-03-20 1990-01-23 Xerox Corporation Transport of suspended charged particles using traveling electrostatic surface waves
US5885527A (en) 1992-05-21 1999-03-23 Biosite Diagnostics, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membrances
US5707799A (en) 1994-09-30 1998-01-13 Abbott Laboratories Devices and methods utilizing arrays of structures for analyte capture
DE19628178C1 (de) 1996-07-12 1997-09-18 Bruker Franzen Analytik Gmbh Verfahren zum Beladen von Probenträgern für Massenspektrometer
DE19742246A1 (de) 1997-09-25 1999-04-01 Basf Ag Analytisches Meßverfahren und seine Verwendung
DE19748295A1 (de) * 1997-10-31 1999-05-06 Max Planck Gesellschaft Element mit extrem stark wasserabweisenden Trockenzonen an der Oberfläche
DE19754978C2 (de) * 1997-12-11 2000-07-13 Bruker Daltonik Gmbh Probenträger für die MALDI-Massenspektrometrie nebst Verfahren zur Herstellung der Platten und zum Aufbringen der Proben
FI980874A7 (fi) * 1998-04-20 1999-10-21 Wallac Oy Menetelmä ja laite pienten nestemäärien kemiallisen analyysin suorittamiseksi
DE19819537A1 (de) * 1998-04-30 2000-03-16 Biochip Technologies Gmbh Analyse- und Diagnostikinstrument
DE19827754C1 (de) * 1998-06-23 2000-02-10 Graffinity Pharm Design Gmbh Einrichtung für eine nahezu gleichzeitige Synthese einer Vielzahl von Proben
FR2783179B1 (fr) 1998-09-16 2000-10-06 Commissariat Energie Atomique Dispositif d'analyse chimique ou biologique comprenant une pluralite de sites d'analyse sur un support, et son procede de fabrication
DE19923761C1 (de) * 1999-05-21 2001-02-08 Bruker Daltonik Gmbh Aufreinigende Probenträger für die MALDI-Massenspektrometrie
SE0000540D0 (sv) 2000-02-18 2000-02-18 Astrazeneca Ab New compounds
US20020031835A1 (en) 2000-05-12 2002-03-14 University Of Delaware Laboratory-on-a-chip device using wetting forces and thermal marangoni pumping
AU2001297014A1 (en) * 2000-10-10 2002-04-22 Aviva Biosciences Corporation An integrated biochip system for sample preparation and analysis
WO2002076878A2 (fr) * 2001-02-09 2002-10-03 Wisconsin Alumni Research Foundation Procede et structure permettant le guidage d'ecoulements microfluidiques
DE10117771A1 (de) * 2001-04-09 2002-10-17 Advalytix Ag Verfahren und Vorrichtung zur Manipulation kleiner Flüssigkeitsmengen und/oder darin enthaltener Teilchen
GB2374818B (en) 2001-04-23 2005-01-12 Secr Defence Surface for promoting droplet formation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001094017A1 (fr) * 2000-06-09 2001-12-13 Advalytix Ag Dispositif et procede permettant de manipuler de petites quantites de matiere
WO2002057014A1 (fr) * 2000-12-14 2002-07-25 Advalytix Ag Procede et dispositif de manipulation de petites quantites de liquides

Also Published As

Publication number Publication date
DE10120035A1 (de) 2002-11-14
WO2002085520A3 (fr) 2003-03-27
WO2002085520A2 (fr) 2002-10-31
US20040180130A1 (en) 2004-09-16
DE10120035B4 (de) 2005-07-07
US7198813B2 (en) 2007-04-03

Similar Documents

Publication Publication Date Title
EP1377364B1 (fr) Dispositif et procede de melange servant a melanger intimement de faibles quantites de liquide
EP1345696B1 (fr) Procede et dispositif de manipulation de petites quantites de liquides
DE10120035B4 (de) Verfahren und Vorrichtung zur Manipulation kleiner Flüssigkeitsmengen auf Oberflächen
EP1286774B1 (fr) Dispositif et procede permettant de manipuler de petites quantites de matiere
DE10326607A1 (de) Vorrichtung zum Handhaben von Flüssigkeiten
WO2004076046A1 (fr) Procede et dispositif pour melanger de petites quantites de liquide dans des microcavites
WO2001072423A1 (fr) Piece pour systeme microfluidique et procede de traitement de la surface d'une telle piece
WO2003056324A2 (fr) Procede de titrage
EP1409722B1 (fr) Procede d'analyse de macromolecules
DE10055318A1 (de) Vorrichtung und Verfahren zum Materietransport kleiner Materiemengen
DE19705910C1 (de) Mikrokammerarray mit hoher Kammerdichte
EP2552586B1 (fr) Composant d'un biocapteur et son procédé de fabrication
DE102004051394B4 (de) Verfahren zur Bewegung von kleinen Flüssigkeitsmengen in Mikrokanälen und Mikrokanalsystem
DE10142788A1 (de) Vorrichtung und Verfahren zur Erzeugung dünner Flüssigkeitsfilme
DE10136008B4 (de) Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung
EP2136921B1 (fr) Procédé et dispositif de manipulation de gouttes
DE10117771A1 (de) Verfahren und Vorrichtung zur Manipulation kleiner Flüssigkeitsmengen und/oder darin enthaltener Teilchen
DE10164358C2 (de) Charakterisierungsverfahren für funktionalisierte Oberflächen
DE10130218A1 (de) Vorrichtung für ein Patch-Clamping von Vesikeln und Verfahren zu deren Herstellung
DE19935028C2 (de) Verfahren und Vorrichtungen zur elektrophoretischen Trennung von Partikeln, insbesondere von Makromolekülen
EP1392437A1 (fr) Systeme de distribution sur puce biocapteur et procede pour la distribution d'une solution a distribuer par utilisation de ce systeme de distribution sur une puce biocapteur
DE10141148B4 (de) Mikrodispenser
DE10063268B4 (de) Vorrichtung und Verfahren zur Vermessung kleiner Flüssigkeitsmengen und/oder deren Bewegung
EP1866089B1 (fr) Dispositif microfluidique pour separer des emulsions
DE10202887B4 (de) Zellanalyseverfahren

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20030904

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ADVALYTIX AG

17Q First examination report despatched

Effective date: 20070216

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20081101