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EP1141380A1 - Controle de l'hygiene - Google Patents

Controle de l'hygiene

Info

Publication number
EP1141380A1
EP1141380A1 EP99961211A EP99961211A EP1141380A1 EP 1141380 A1 EP1141380 A1 EP 1141380A1 EP 99961211 A EP99961211 A EP 99961211A EP 99961211 A EP99961211 A EP 99961211A EP 1141380 A1 EP1141380 A1 EP 1141380A1
Authority
EP
European Patent Office
Prior art keywords
glucose
sample
hygiene monitoring
atp
reagents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99961211A
Other languages
German (de)
English (en)
Inventor
Peter Leonard Grant
Ramin Pirzad
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Celsis International PLC
Original Assignee
Celsis International PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celsis International PLC filed Critical Celsis International PLC
Publication of EP1141380A1 publication Critical patent/EP1141380A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Definitions

  • This invention relates to hygiene monitoring, and in particular to an assay for the presence of food residues and/or microorganisms.
  • Hygiene monitoring procedures typically involve swabbing a surface, and analysing the sample taken up in the swab, for the presence of food residues and/or microorganisms.
  • a typical hygiene monitoring device is disclosed in WO-A-98/27196.
  • the ability to detect the presence of food residues and/or microorganisms in the sample that has been taken up by swabbing relies on the presence of ADP/ATP, and the ability to amplify the amount of ADP/ATP, and convert it to a detectable signal, also as disclosed in WO-A-94/25619.
  • the preferred amplification reaction involves, inter alia, the amplification of ATP and then the conversion of glucose-6-phosphate to glucose, and the conversion of glucose, via a sequence of enzymatic reactions, to a coloured end point.
  • glucose itself may be a sufficient indicator, for the purposes of an assay used in hygiene monitoring.
  • such an assay comprises the collection of a sample from a locus, and the determination of the presence of glucose in the sample.
  • the reagents disclosed in WO-A-94/25619 include those sufficient to convert glucose to a detectable signal; if desired, the reagents used in this invention may exclude those that convert ATP, but the signal generated in the presence of glucose appears relatively rapidly, and can thus be distinguished, in addition to the advantage of providing a rapid response. The amplification of ATP needed to generate the signal is relatively slow.
  • the locus to be tested is also assayed for the substantial absence of materials that may interfere in the novel assay, e.g. by inhibiting or by giving false positives, such as peroxide or reducing agents that may be incorporated in materials used to sanitise the locus.
  • the present invention can be practised utilising the same reagents as are disclosed in WO-A-94/25619, especially in so far as that relates to the conversion of glucose to a detectable signal. If desired, the reagents may exclude one or more of the components that are disclosed there for the conversion of ATP to glucose.
  • a suitable device that can be used for the purposes of swabbing and detection is disclosed in WO-A-98/27196 and WO-A-99/31218.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Swabs were taken from a variety of locations, and tested. In addition to determining the total viable count (TVC) and Enterobacteria (Enteros), contact plates were used alongside two devices.
  • systemSURE (“sSURE”) is a portable hygiene monitoring system (available from Becton Dickinson) that uses bioluminescence as an endpoint.
  • the "Pen Swab” is generally as disclosed in WO-A-99/31218. Results are shown in Table 1.
  • the colour reagents were used to determine the level at which glucose was detectable.
  • the reagents were activated in the presence of different concentrations of glucose.
  • the absorbance values for a complete colour change were obtained within 120 seconds. Results are shown in Table 2.
  • Swabs were taken from a variety of locations in a store, at different times of day and tested. Results are shown, for two different days, in Tables 3A and 3B. A large proportion of the data shows a rapid colour change (in seconds), characteristic of glucose detection. The corresponding RLU values do not show the presence of gross amounts of ATP, which is consistent with the detection of glucose. Table 3A

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Emergency Medicine (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne une technique de contrôle de l'hygiène, consistant à collecter un échantillon sur un site, et à déterminer la présence de glucose dans ledit échantillon.
EP99961211A 1998-12-15 1999-12-15 Controle de l'hygiene Withdrawn EP1141380A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9827584 1998-12-15
GBGB9827584.5A GB9827584D0 (en) 1998-12-15 1998-12-15 Hygiene Monitoring
PCT/GB1999/004244 WO2000036139A1 (fr) 1998-12-15 1999-12-15 Controle de l'hygiene

Publications (1)

Publication Number Publication Date
EP1141380A1 true EP1141380A1 (fr) 2001-10-10

Family

ID=10844247

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99961211A Withdrawn EP1141380A1 (fr) 1998-12-15 1999-12-15 Controle de l'hygiene

Country Status (5)

Country Link
EP (1) EP1141380A1 (fr)
JP (1) JP2002532698A (fr)
AU (1) AU1789700A (fr)
GB (1) GB9827584D0 (fr)
WO (1) WO2000036139A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0100770D0 (en) * 2001-01-11 2001-02-21 Celsis Internat Plc Hygiene monitoring
US7229783B2 (en) 2000-08-01 2007-06-12 Charm Sciences, Inc. Method for monitoring hygiene
CA2469025A1 (fr) 2001-12-06 2003-06-19 Biocontrol Systems, Inc. Systeme de recuperation et d'analyse d'echantillons
US20030209653A1 (en) 2002-04-24 2003-11-13 Biocontrol Systems, Inc. Sample collection and testing system
US9446406B2 (en) 2012-06-29 2016-09-20 Biocontrol Systems, Inc. Sample collection and bioluminescent analysis system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9308411D0 (en) * 1993-04-23 1993-06-09 Celsis Ltd Detection of biological material
US8349602B1 (en) * 1996-04-19 2013-01-08 Xenogen Corporation Biodetectors targeted to specific ligands

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0036139A1 *

Also Published As

Publication number Publication date
JP2002532698A (ja) 2002-10-02
WO2000036139A1 (fr) 2000-06-22
GB9827584D0 (en) 1999-02-10
AU1789700A (en) 2000-07-03

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