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EP0732585A3 - Multi-channel electrophoretic separation device - Google Patents

Multi-channel electrophoretic separation device Download PDF

Info

Publication number
EP0732585A3
EP0732585A3 EP96301807A EP96301807A EP0732585A3 EP 0732585 A3 EP0732585 A3 EP 0732585A3 EP 96301807 A EP96301807 A EP 96301807A EP 96301807 A EP96301807 A EP 96301807A EP 0732585 A3 EP0732585 A3 EP 0732585A3
Authority
EP
European Patent Office
Prior art keywords
cavities
plates
fluid
separation device
output ports
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96301807A
Other languages
German (de)
French (fr)
Other versions
EP0732585A2 (en
Inventor
Milan Bier
Terry D. Long
Rodolfo Marquez
Anthony R. Ford
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gyros Protein Technologies AB
Original Assignee
Protein Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protein Technologies Inc filed Critical Protein Technologies Inc
Publication of EP0732585A2 publication Critical patent/EP0732585A2/en
Publication of EP0732585A3 publication Critical patent/EP0732585A3/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
    • B01D35/06—Filters making use of electricity or magnetism
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/42—Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
    • B01D61/44—Ion-selective electrodialysis
    • B01D61/46—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/087—Single membrane modules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrostatic Separation (AREA)

Abstract

A multi-channel separation device (10) is provided for use in separation technology, particularly in electrophoresis and in isoelectric focusing. The device (10) includes two mating plates (12,14), at least one of which contains an array of at least three cavities (16-34) arranged to define a serpentine fluid pathway through the cavities when the plates are mated. Each of the cavities (16-34) includes fluid input and output ports to facilitate transfer of process fluid through the cavities. A septum (40) is disposed between the plates (12,14) for streamlining fluid flow between the input and output ports by restricting but not preventing free fluid flow between adjacent cavities (16-34). The device (10) also includes anodic and cathodic compartments containing electrodes (50,52) for establishing an electric field across the cavities.
EP96301807A 1995-03-15 1996-03-15 Multi-channel electrophoretic separation device Withdrawn EP0732585A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/403,982 US5540826A (en) 1995-03-15 1995-03-15 Multi-channel separation device
US403982 1995-03-15

Publications (2)

Publication Number Publication Date
EP0732585A2 EP0732585A2 (en) 1996-09-18
EP0732585A3 true EP0732585A3 (en) 1996-10-02

Family

ID=23597645

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96301807A Withdrawn EP0732585A3 (en) 1995-03-15 1996-03-15 Multi-channel electrophoretic separation device

Country Status (2)

Country Link
US (1) US5540826A (en)
EP (1) EP0732585A3 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153076A (en) * 1998-01-12 2000-11-28 The Regents Of The University Of California Extended length microchannels for high density high throughput electrophoresis systems
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
WO2001068225A1 (en) * 2000-03-15 2001-09-20 Proteosys Ag Micropreparative isoelectric focussing
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
DE10047088C2 (en) * 2000-09-21 2002-10-17 Gerhard Weber Medium for analytical and preparative electrophoresis
DE10063097B4 (en) * 2000-12-18 2007-04-19 Becton, Dickinson And Co. electrophoresis
DE10063096C1 (en) * 2000-12-18 2002-09-12 Gerhard Weber Electrophoresis device, electrophoresis method using an electrophoresis device and using the electrophoresis device
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
WO2004077017A2 (en) * 2003-02-21 2004-09-10 West Virginia University Research Corporation Apparatus and method for on-chip concentration using a microfluidic device with an integrated ultrafiltration membrane structure
US6793791B2 (en) * 2003-02-26 2004-09-21 Milan Bier Variable-volume disposable isoelectric focusing cell and method of isoelectric focusing
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
RU2232952C1 (en) * 2003-06-03 2004-07-20 Алиева Елена Антоновна Method of heating and cooling fluid medium
DE102005020134A1 (en) 2005-04-29 2006-11-02 Becton, Dickinson And Co. Electrophoresis method, e.g. for analysis and preparation separation of ions, peptides, involves providing boundaries defining separation sub-spaces by adjacent flow of anodic and cathodic stabilization media through respective inlets
EP1963817A2 (en) * 2005-12-22 2008-09-03 Honeywell International Inc. Portable sample analyzer cartridge
EP2146200A1 (en) * 2008-07-16 2010-01-20 Agilent Technologies, Inc. Device and method for isoelectric focusing
WO2017112023A2 (en) * 2015-10-19 2017-06-29 Massachusetts Institute Of Technology Solid state pump using elecro-rheological fluid
US10761054B2 (en) * 2016-05-13 2020-09-01 Agilent Technologies, Inc. Systems and methods for automated alignment, calibration and standardization of electrophoresis data

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4362612A (en) * 1978-04-18 1982-12-07 University Patents, Inc. Isoelectric focusing apparatus
US4401538A (en) * 1981-06-02 1983-08-30 Hausfeld David A Isoelectric focusing techniques and devices
EP0376611A2 (en) * 1988-12-30 1990-07-04 The Board Of Trustees Of The Leland Stanford Junior University Electrophoretic system
US4963236A (en) * 1989-03-08 1990-10-16 Ampholife Technologies Apparatus and methods for isoelectric focusing
US5256269A (en) * 1990-12-14 1993-10-26 908098 Ontario Inc. Method and apparatus for separating biological substances and organic compounds in solution
EP0682248A1 (en) * 1993-11-29 1995-11-15 Toray Research Center, Inc. Electrophoresis fractionator

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204929A (en) * 1978-04-18 1980-05-27 University Patents, Inc. Isoelectric focusing method
DE3876273T2 (en) * 1987-04-11 1993-05-27 Ciba Geigy Ag ISOELECTRIC FOCUSING METHOD AND DEVICE FOR CARRYING OUT THIS METHOD.
GB2225339A (en) * 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
US5173164A (en) * 1990-09-11 1992-12-22 Bioseparations, Inc. Multi-modality electrical separator apparatus and method
US5411650A (en) * 1993-07-21 1995-05-02 Eastman Kodak Company Captive vortex high agitation device

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4362612A (en) * 1978-04-18 1982-12-07 University Patents, Inc. Isoelectric focusing apparatus
US4401538A (en) * 1981-06-02 1983-08-30 Hausfeld David A Isoelectric focusing techniques and devices
EP0376611A2 (en) * 1988-12-30 1990-07-04 The Board Of Trustees Of The Leland Stanford Junior University Electrophoretic system
US4963236A (en) * 1989-03-08 1990-10-16 Ampholife Technologies Apparatus and methods for isoelectric focusing
US5256269A (en) * 1990-12-14 1993-10-26 908098 Ontario Inc. Method and apparatus for separating biological substances and organic compounds in solution
EP0682248A1 (en) * 1993-11-29 1995-11-15 Toray Research Center, Inc. Electrophoresis fractionator

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BELFORT G ET AL: "An experimental study of electrodialysis hydrodynamics", DESALINATION, MAY 1972, NETHERLANDS, vol. 10, no. 3, ISSN 0011-9164, pages 221 - 262, XP002008297 *

Also Published As

Publication number Publication date
EP0732585A2 (en) 1996-09-18
US5540826A (en) 1996-07-30

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