EP0513709A2 - Procédé enzymatique pour réduire la teneur en composants contenant du phosphore dans les huiles végétales et animales - Google Patents

Procédé enzymatique pour réduire la teneur en composants contenant du phosphore dans les huiles végétales et animales Download PDF

Info

Publication number: EP0513709A2
Authority: EP; European Patent Office
Prior art keywords: oil; phospholipase; degummed; ppm; content
Prior art date: 1991-05-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP92107888A

Other languages

German (de)

English (en)

Other versions

EP0513709A3 (en

EP0513709B2 (fr

EP0513709B1 (fr

Inventor

Erik Aalrust

Wolfgang Beyer

Hans Ottofrickenstein

Georg Penk

Hermann Dr. Plainer

Roland Dr. Reiner

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

AB Enzymes GmbH

Roehm GmbH Darmstadt

Original Assignee

Metallgesellschaft AG

Roehm GmbH Darmstadt

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-05-16

Filing date

1992-05-11

Publication date

1992-11-19

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6431742&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP0513709(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1992-05-11 Application filed by Metallgesellschaft AG, Roehm GmbH Darmstadt filed Critical Metallgesellschaft AG

1992-11-19 Publication of EP0513709A2 publication Critical patent/EP0513709A2/fr

1992-12-30 Publication of EP0513709A3 publication Critical patent/EP0513709A3/de

1995-03-29 Application granted granted Critical

1995-03-29 Publication of EP0513709B1 publication Critical patent/EP0513709B1/fr

1999-10-06 Publication of EP0513709B2 publication Critical patent/EP0513709B2/fr

2012-05-11 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead

Definitions

the NHP arise from the action of the plant's own enzymes. In the "Alcon process", these are inactivated by steam treatment of the soy flakes, so that the formation of the NHP is prevented and the phosphatide content can be almost completely removed when the crude oil is degummed.
Phospholipase A2 and B are available as commercial products.
the use of purified enzymes is generally not necessary for technical applications.
a phospholipase preparation which is obtained from ground pancreatic pulp and contains above all phospholipase A2, is suitable for the process of the invention.
the enzyme is used in amounts of 0.001 to 1% by weight, based on oil.
a good distribution of the enzyme in the oil is ensured if it is dissolved in an amount of water of 0.5 to 5% by weight, based on oil, and emulsified in this form in the oil into droplets of less than 10 micrometers in diameter (weight average) becomes.
Turbulent stirring at radial speeds above 100 cm / s has proven itself. Instead, the oil can be circulated in the reactor using an external centrifugal pump. The enzymatic reaction can also be promoted by means of ultrasound.
emulsifying additives are helpful.
Water-soluble emulsifiers are suitable, in particular those with an HLB value above 9, such as sodium dodecyl sulfate. It is effective in an amount of, for example, 0.001% by weight based on oil if it is added to the enzyme solution prior to emulsification in the oil.
1 l wet-degummed soybean oil with a residual phosphorus content of 110 ppm is heated to 75 o C in a round bottom flask. With vigorous stirring using a 5 cm diameter paddle stirrer at 700 rpm, 10 ml of water containing 1 g of citric acid are added and stirring is continued for 1 hour. Then it is cooled to 40 ° C. and a solution of 0.1 g of phospholipase A2 of the quality given in Example 1 and 50 mg of calcium chloride in 20 ml of 0.1 molar acetate buffer pH 5.5 are added. After a further 5 hours of intensive stirring, the water phase is centrifuged off. The oil obtained contains 2 ppm P and is suitable for physical refining.

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Microbiology (AREA)
Oil, Petroleum & Natural Gas (AREA)
Engineering & Computer Science (AREA)
Chemical Kinetics & Catalysis (AREA)
Biochemistry (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Edible Oils And Fats (AREA)
Fats And Perfumes (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

EP92107888A 1991-05-16 1992-05-11 Procédé enzymatique pour réduire la teneur en composants contenant du phosphore dans les huiles végétales et animales Expired - Lifetime EP0513709B2 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE4115938		1991-05-16
DE4115938A DE4115938A1 (de)	1991-05-16	1991-05-16	Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen

Publications (4)

Publication Number	Publication Date
EP0513709A2 true EP0513709A2 (fr)	1992-11-19
EP0513709A3 EP0513709A3 (en)	1992-12-30
EP0513709B1 EP0513709B1 (fr)	1995-03-29
EP0513709B2 EP0513709B2 (fr)	1999-10-06

Family

ID=6431742

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP92107888A Expired - Lifetime EP0513709B2 (fr)	1991-05-16	1992-05-11	Procédé enzymatique pour réduire la teneur en composants contenant du phosphore dans les huiles végétales et animales

Country Status (15)

Country	Link
US (1)	US5264367A (fr)
EP (1)	EP0513709B2 (fr)
CN (1)	CN1034587C (fr)
AR (1)	AR245193A1 (fr)
AT (1)	ATE120482T1 (fr)
BR (1)	BR9201859A (fr)
CA (1)	CA2068933C (fr)
DE (2)	DE4115938A1 (fr)
DK (1)	DK0513709T4 (fr)
ES (1)	ES2072043T5 (fr)
GR (2)	GR3015920T3 (fr)
HU (1)	HU213754B (fr)
PL (1)	PL170548B1 (fr)
RU (1)	RU2033422C1 (fr)
TW (1)	TW203625B (fr)

Cited By (26)

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EP0622446A3 (fr) *	1993-04-25	1995-02-01	Showa Sangyo Co	Procédé de raffinage d'huile et de graisse.
DE4339556C1 (de) *	1993-11-19	1995-02-02	Metallgesellschaft Ag	Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
WO1997005219A1 (fr) *	1995-07-26	1997-02-13	Röhm Gmbh	PROCEDE DE DEMUCILAGINATION PAR VOIE ENZYMATIQUE D'HUILES VEGETALES AU MOYEN DE PHOSPHOLIPASE D'$i(ASPERGILLUS)
WO1998018912A1 (fr) *	1996-10-31	1998-05-07	Novo Nordisk A/S	Nouvelle phospholipase, sa production et son utilisation
US6025171A (en) *	1996-12-19	2000-02-15	Lipton, Division Of Conopco, Inc.	Immobilizing enzymes and processing triglycerides with immobilized lipase
US6127137A (en) *	1996-10-31	2000-10-03	Novo Nordisk A/S	Acidic phospholipase, production and methods using thereof
US6162623A (en) *	1995-06-27	2000-12-19	Lipton, Division Of Conopco, Inc.	Processes for preparing and using immobilized lipases
WO2003089620A2 (fr)	2002-04-19	2003-10-30	Diversa Corporation	Phospholipases, acides nucleiques codant pour ces phosphalipases et methodes de fabrication et d'utilisation
WO2006009676A2 (fr)	2004-06-16	2006-01-26	Diversa Corporation	Compositions et procedes pour la decoloration enzymatique de la chlorophylle
US7226771B2 (en)	2002-04-19	2007-06-05	Diversa Corporation	Phospholipases, nucleic acids encoding them and methods for making and using them
WO2008036863A2 (fr)	2006-09-21	2008-03-27	Verenium Corporation	Phospholipases, acides nucléiques codant pour elles et leurs méthodes d'obtention et d'utilisation
WO2009046988A3 (fr) *	2007-10-10	2009-06-18	Chr Hansen As	Procédé de production d'une émulsion eau dans huile
WO2010025395A2 (fr)	2008-08-29	2010-03-04	Verenium Corporation	Hydrolase, acides nucléiques les codant et procédés de fabrication et d’utilisation associés
EP2216403A2 (fr)	2006-02-02	2010-08-11	Verenium Corporation	Estérases et acides nucléiques associés et procédés
WO2011046815A1 (fr)	2009-10-16	2011-04-21	Bunge Oils, Inc.	Procédés de démucilagination d'huile
WO2011046812A1 (fr)	2009-10-16	2011-04-21	Verenium Corporation	Phospholipases, acides nucléiques codant pour celles-ci et leurs procédés de fabrication et d'utilisation
WO2011051322A1 (fr)	2009-10-28	2011-05-05	Ab Enzymes Gmbh	Clonage, expression et utilisation de phospholipases acides
US7943360B2 (en)	2002-04-19	2011-05-17	Verenium Corporation	Phospholipases, nucleic acids encoding them and methods for making and using them
WO2012001153A1 (fr) *	2010-07-01	2012-01-05	Süd-Chemie AG	Complexe phospholipase/substrat
USRE43341E1 (en)	1995-06-07	2012-05-01	Danisco A/S	Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US8202715B2 (en)	2006-10-02	2012-06-19	Ab Enzymes Gmbh	Cloning, expression and use of acid lysophospholipases
US8557551B2 (en)	2004-09-10	2013-10-15	Dsm Ip Assets B.V.	Compositions and methods for making and modifying oils
EP2853593A1 (fr)	2003-03-07	2015-04-01	DSM IP Assets B.V.	Hydrolases, acides nucléiques les codant et leurs procédés de fabrication et d'utilisation
US9017990B2 (en)	2004-09-10	2015-04-28	Dsm Ip Assets B.V.	Methods for enzymatic decolorization of chlorophyll
WO2015173426A1 (fr)	2014-05-15	2015-11-19	Novozymes A/S	Compositions comprenant des polypeptides ayant une activité phospholipase c et leur utilisation
EP4163354A2 (fr)	2014-03-19	2023-04-12	Novozymes A/S	Polypeptides présentant une activité phospholipase c et polynucléotides codant pour ceux-ci

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JP3824174B2 (ja)	1996-12-09	2006-09-20	ノボザイムスアクティーゼルスカブ	ホスホリパーゼの使用による高い量の非水和性リンを含む食用油中のリン含有成分の減少、ホスホリパーゼａおよび／またはｂ活性を有する糸状菌からのホスホリパーゼ
US6103505A (en) *	1996-12-09	2000-08-15	Novo Nordisk A/S	Method for reducing phosphorus content of edible oils
DE1193314T1 (de)	1997-04-09	2002-10-02	Danisco A/S, Copenhagen	Lipase und Verwendung davon zur Verbesserung von Teigen und Backwaren
CN1139652C (zh) *	1998-04-08	2004-02-25	诺维信公司	一种酶促油脱胶的方法
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JP4723087B2 (ja)	1998-11-27	2011-07-13	ノボザイムスアクティーゼルスカブ	脂質分解酵素変異体
US7312062B2 (en) *	1998-11-27	2007-12-25	Novozymes A/S	Lipolytic enzyme variants
BRPI0009001B1 (pt) *	1999-03-16	2017-06-06	Novozymes As	processo para a produção de queijo
US6464875B1 (en)	1999-04-23	2002-10-15	Gold Kist, Inc.	Food, animal, vegetable and food preparation byproduct treatment apparatus and process
AU2001254620A1 (en)	2000-04-28	2001-11-12	Novozymes A/S	Lipolytic enzyme variant
WO2002000852A2 (fr)	2000-06-26	2002-01-03	Novozymes A/S	Lipase
NZ528260A (en)	2001-05-18	2005-09-30	Danisco	Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
JP4511342B2 (ja)	2002-05-21	2010-07-28	ディーエスエムアイピーアセッツビー．ブイ．	新規なホスホリパーゼおよびその使用
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EP1711587B1 (fr)	2003-12-19	2013-04-10	Bunge Oils, Inc	Amelioration du degommage enzymatique d'huiles vegetales et encrassement reduit des installations de traitement aval
US7906307B2 (en)	2003-12-24	2011-03-15	Danisco A/S	Variant lipid acyltransferases and methods of making
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GB0716126D0 (en)	2007-08-17	2007-09-26	Danisco	Process
GB0405637D0 (en)	2004-03-12	2004-04-21	Danisco	Protein
AU2005264077B2 (en)	2004-07-16	2011-06-02	Dupont Nutrition Biosciences Aps	Lipolytic enzyme uses thereof in the food industry
US8012724B2 (en) *	2005-06-13	2011-09-06	Novozymes A/S	Production of degummed fatty acid alkyl esters using both lipase and phospholipase in a reaction mixture
US20070148311A1 (en) *	2005-12-22	2007-06-28	Bunge Oils, Inc.	Phytosterol esterification product and method of make same
DE102006046719A1 (de)	2006-10-02	2008-04-03	Ab Enzymes Gmbh	Klonierung, Expression und Verwendung saurer Phospholipasen
BRPI0720801A2 (pt)	2007-01-25	2014-09-16	Dupont Nutrition Biosci Aps	Produção de um lipídio aciltranfersase a partir de células transformadas de bacillus licheniformis.
US8460905B2 (en) *	2007-09-11	2013-06-11	Bunge Oils, Inc.	Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time
US8956853B2 (en) *	2007-01-30	2015-02-17	Bunge Oils, Inc.	Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases
US8241876B2 (en)	2008-01-07	2012-08-14	Bunge Oils, Inc.	Generation of triacylglycerols from gums
US8357503B2 (en)	2008-08-29	2013-01-22	Bunge Oils, Inc.	Hydrolases, nucleic acids encoding them and methods for making and using them
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DE102009006920B4 (de)	2009-02-02	2016-03-17	Air Liquide Global E&C Solutions Germany Gmbh	Verfahren zur Vermeidung von Sterolglycoside enthaltenden Ausfällungen bei der Herstellung von Fettsäurealkylestern
DE102009006921A1 (de)	2009-02-02	2010-08-05	Lurgi Gmbh	Verfahren, Anlage und Mittel zur Vermeidung von Sterolglycoside enthaltenden Ausfällungen bei der Herstellung von Fettsäurealkylestern
GB0904787D0 (en) *	2009-03-20	2009-05-06	Desmet Ballestra Engineering Sa	Improved enzymatic oil recuperation process
AR077042A1 (es)	2009-06-12	2011-07-27	Danisco	Metodo para tratar una composicion que contiene pirofiofitina
CA2788148C (fr)	2010-03-12	2018-03-13	Dupont Nutrition Biosciences Aps	Un procede enzymatique de raffinage d'huile vegetale
BR112012032176A2 (pt)	2010-06-17	2015-09-08	Dupont Nutrition Biosci Aps	processo de tratamento de sementes que contém óleo, método de obtenção de óleo de sementes vegetais, processo de produção de um óleo vegetal refinado e óleo vegetal bruto ou refinado que pode ser obtido por meio de um método ou processo
CA2812751C (fr)	2010-09-30	2018-05-01	National University Corporation Tokyo University Of Marine Science And Technology	Composition contenant de la 2-acyl-lysophosphatidylserine et son procede de production
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WO2013104660A1 (fr)	2012-01-13	2013-07-18	Dupont Nutrition Biosciences Aps	Procédé de traitement d'une huile végétale comprenant l'hydrolyse de chlorophylle ou d'un dérivé de chlorophylle et une neutralisation caustique partielle
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ES2713402T3 (es)	2012-06-14	2019-05-21	Bunge Global Innovation Llc	Procedimiento de producción de aceites de saturación baja
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EP2799531A1 (fr)	2013-05-03	2014-11-05	Clariant Produkte (Deutschland) GmbH	Utilisation de phosphatases pour la démucilagination enzymatique de triglycérides
US10294463B2 (en)	2013-07-03	2019-05-21	Keclon Sa	Modified Bacillus cereus phospholipase C protein and method of processing vegetable oil
EP2910129A1 (fr)	2014-02-21	2015-08-26	Clariant Produkte (Deutschland) GmbH	Composition pour la démucilagination enzymatique d'huiles
HUE070566T2 (hu)	2016-06-16	2025-06-28	Novozymes As	Foszfolipidek csökkentése foszfolipidet tartalmazó olajos anyagban
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1991
- 1991-05-16 DE DE4115938A patent/DE4115938A1/de not_active Withdrawn
1992
- 1992-05-11 AT AT92107888T patent/ATE120482T1/de active
- 1992-05-11 DK DK92107888T patent/DK0513709T4/da active
- 1992-05-11 EP EP92107888A patent/EP0513709B2/fr not_active Expired - Lifetime
- 1992-05-11 ES ES92107888T patent/ES2072043T5/es not_active Expired - Lifetime
- 1992-05-11 DE DE59201753T patent/DE59201753D1/de not_active Expired - Lifetime
- 1992-05-12 CN CN92103459A patent/CN1034587C/zh not_active Expired - Lifetime
- 1992-05-14 US US07/882,710 patent/US5264367A/en not_active Expired - Lifetime
- 1992-05-14 RU SU925011752A patent/RU2033422C1/ru active
- 1992-05-14 PL PL92294543A patent/PL170548B1/pl unknown
- 1992-05-15 AR AR92322337A patent/AR245193A1/es active
- 1992-05-15 TW TW081103810A patent/TW203625B/zh not_active IP Right Cessation
- 1992-05-15 HU HU9201630A patent/HU213754B/hu unknown
- 1992-05-15 BR BR929201859A patent/BR9201859A/pt not_active Application Discontinuation
- 1992-05-19 CA CA002068933A patent/CA2068933C/fr not_active Expired - Lifetime
1995
- 1995-04-25 GR GR950401041T patent/GR3015920T3/el unknown
1999
- 1999-11-10 GR GR990402897T patent/GR3031804T3/el unknown

Cited By (43)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5532163A (en) *	1993-04-25	1996-07-02	Showa Sangyo Co., Ltd.	Process for refining oil and fat
EP0622446A3 (fr) *	1993-04-25	1995-02-01	Showa Sangyo Co	Procédé de raffinage d'huile et de graisse.
DE4339556C1 (de) *	1993-11-19	1995-02-02	Metallgesellschaft Ag	Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
EP0654527A1 (fr) *	1993-11-19	1995-05-24	Metallgesellschaft Aktiengesellschaft	Procédé de démucilagination d'huiles végetales avec des enzymes
US5558781A (en) *	1993-11-19	1996-09-24	Metallgesellschaft Aktiengesellschaft	Process for enzymatically degumming vegetable oil
USRE43341E1 (en)	1995-06-07	2012-05-01	Danisco A/S	Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6162623A (en) *	1995-06-27	2000-12-19	Lipton, Division Of Conopco, Inc.	Processes for preparing and using immobilized lipases
WO1997005219A1 (fr) *	1995-07-26	1997-02-13	Röhm Gmbh	PROCEDE DE DEMUCILAGINATION PAR VOIE ENZYMATIQUE D'HUILES VEGETALES AU MOYEN DE PHOSPHOLIPASE D'$i(ASPERGILLUS)
US6001640A (en) *	1995-07-26	1999-12-14	Roehm Gmbh	Vegetable oil enzymatic degumming process by means of aspergillus phospholipase
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Also Published As

Publication number	Publication date
US5264367A (en)	1993-11-23
CA2068933C (fr)	1995-12-19
DE4115938A1 (de)	1992-11-19
RU2033422C1 (ru)	1995-04-20
HU213754B (en)	1997-09-29
TW203625B (fr)	1993-04-11
PL170548B1 (pl)	1996-12-31
DK0513709T3 (da)	1995-07-24
EP0513709A3 (en)	1992-12-30
HU9201630D0 (en)	1992-08-28
ES2072043T3 (es)	1995-07-01
CA2068933A1 (fr)	1992-11-17
ES2072043T5 (es)	2000-02-01
EP0513709B2 (fr)	1999-10-06
PL294543A1 (en)	1993-01-25
AR245193A1 (es)	1993-12-30
CN1034587C (zh)	1997-04-16
EP0513709B1 (fr)	1995-03-29
BR9201859A (pt)	1993-01-05
ATE120482T1 (de)	1995-04-15
GR3031804T3 (en)	2000-02-29
GR3015920T3 (en)	1995-07-31
HUT64578A (en)	1994-01-28
DE59201753D1 (de)	1995-05-04
CN1066679A (zh)	1992-12-02
DK0513709T4 (da)	1999-12-27

Publication	Publication Date	Title
EP0513709B1 (fr)	1995-03-29	Procédé enzymatique pour réduire la teneur en composants contenant du phosphore dans les huiles végétales et animales
DE69408891T2 (de)	1998-10-22	Verfahren zum Raffinieren von Öl und Fett
EP0906379B1 (fr)	2003-04-23	Procede de demucilagination par voie enzymatique d'huiles vegetales au moyen de phospholipase d'aspergillus
DE602004002866T2 (de)	2007-05-24	Verfahren zum Rückgewinnen von Öl
DE2948367C2 (fr)	1989-03-16
US5696278A (en)	1997-12-09	Degumming of crude glyceride oils not exposed to prior enzymatic activity
US4162260A (en)	1979-07-24	Oil purification by adding hydratable phosphatides
DE2946565A1 (de)	1980-06-04	Verfahren zur herstellung von kakaobutterersatz
EP0269277B1 (fr)	1991-07-24	Procédé de démucilagination d'huiles triglycéridiques
DE69200004T2 (de)	1993-09-09	Verfahren zur kontinuierlichen entschleimung eines glyceridoeles.
DE69716080T2 (de)	2003-03-13	Verfahren zur herstellung von glyceridöl mit geringem gehalt an nicht-hydratisierbaren phosphatiden
JP4064548B2 (ja)	2008-03-19	米ぬか油の製造方法
EP2792735A1 (fr)	2014-10-22	Procédé d'amélioration de la démucilagination enzymatique aqueuse d'huiles végétales
EP0583648A2 (fr)	1994-02-23	Procédé de raffinage en continu avec des effluents réduits
WO2014177279A1 (fr)	2014-11-06	Utilisation de phosphatases pour la démucilagination enzymatique de triglycérides
IE47286B1 (en)	1984-02-08	Improvements in or relating to the degumming of triglyceride oils
DD267055A1 (de)	1989-04-19	Verfahren zur gewinnung grenzflaechenaktiver lipide
EP0058712A1 (fr)	1982-09-01	Procede de purification d'huiles vegetales, animales et marines de triglycerides brutes ou partiellement purifiees
DE2104259A1 (en)	1972-08-10	Oil-extraction from seeds - esp grains, by enzymatic breakdown of cell walls in water dispersions

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