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EP0579635A1 - Micro-organismes antifongiques - Google Patents

Micro-organismes antifongiques

Info

Publication number
EP0579635A1
EP0579635A1 EP92907473A EP92907473A EP0579635A1 EP 0579635 A1 EP0579635 A1 EP 0579635A1 EP 92907473 A EP92907473 A EP 92907473A EP 92907473 A EP92907473 A EP 92907473A EP 0579635 A1 EP0579635 A1 EP 0579635A1
Authority
EP
European Patent Office
Prior art keywords
antifungal agent
plant
antifungal
ncib
fungus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP92907473A
Other languages
German (de)
English (en)
Inventor
Annabel Renwick
Keith Adrian Powell
Richard Campbell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Zeneca Ltd
Imperial Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Imperial Chemical Industries Ltd filed Critical Zeneca Ltd
Publication of EP0579635A1 publication Critical patent/EP0579635A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/39—Pseudomonas fluorescens

Definitions

  • This invention relates to an agricultural plant protection product. More specifically, the invention relates to an antifungal micro-organism, in particular to a novel strain of Pseudomonas fluorescens, and the uses thereof for the protection of plants against fungal attack.
  • Certain strains of the micro-organism Pseudomonas fluorescens are known to be useful agricultural fungicides.
  • the antifungal activity varies greatly amongst the innumerable strains of the micro-organism.
  • Agriculturally useful strains are those which possess useful levels of activity against specific target fungi.
  • One such target is the group of fungi which are associated with the disease known as "damping-off" which is particularly troublesome in certain crops and under particular climatic conditions.
  • Use of Biological Control Agents (BCAs) or biopesticides to control disease may be more effective or more preferable to the use of standard chemical control agents.
  • An object of the present invention is to provide an agriculturally useful fungicide.
  • the invention also comprises an antifungal agricultural composition containing Pseudomonas fluorescens Biovar I, strain NCIB 40189 as active ingredient in admixture with a carrier composition acceptable in agricultural practice.
  • a carrier composition acceptable in agricultural practice examples of the types of agricultural formulations which may be employed are seed coatings compositions, liquid for root or soil drenching and granular or powder compositions. The base materials for these are well known in the art.
  • the invention provides a method of protecting crop plants from fungal infection comprising applying to the plants, the roots or seeds thereof or the growing medium for the plant a fungicidally effective dosage of the said Pseudomonas fluorescens Biovar I, strain NCIB 40189.
  • the treatment may be effected by application of a preparation containing organisms of the strain of this invention.
  • the antifungal micro-organism of this invention is particularly effective against fungal infections associated with "damping-off" disease, such as fungi of the genera Rhizoctonia, Pythium, Fusarium. It is also active against
  • Gaeumannomyces graminis which causes take-all disease.
  • the strain of this invention (NCIB 40189) was isolated from volunteer wheat roots collected from plants grown at Chiseldon, Swindon, Wiltshire,
  • the bacterium was isolated from volunteer wheat roots dug from a field at a farm known as Draycott Farm, Chiseldon, Swindon, Wiltshire, United Kingdom.
  • the roots were washed to remove all adhering soil and placed in sterile distilled water in a flask containing glass beads.
  • the roots were shaken with the glass beads for 15 minutes and the washings then plated out on to Kings B agar (Proteose Peptone No.3, 20g; glycerol 10ml; potassium sulphate, 1.5g; magnesium sulphate heptahydrate, 1.5g; technical agar, 12g; and, one litre of distilled water at pH 7.2 and including cycloheximide 75 micrograms/ml; chloromycetin 12.5 micrograms/ml; and ampicillin 50 micrograms/ml) at 10:1 and 10:3 dilutions.
  • the plates were incubated for three days at 20 ⁇ C.
  • the bacterium was taken at random from the agar plate and sub-cultured on to 1/10 tryptic soya broth agar (TSA) (3g tryptic soya broth; 15g agar; one litre water). The bacterium was maintained an 1/10 TSA and stored at 4°C until required.
  • TSA tryptic soya broth agar
  • Phenylalanine deaminase - Growth factor required
  • Pseudomonas fluorescens strain NCIB 40189 was tested for activity against Pythium ultimum, using a petri-dish soil screen.
  • Petri-dishes (5cm) were filled with 10ml of potato dextrose agar (PDA).
  • Minster Mendip Loam was autoclaved at 120°C for 60 minutes. To this sterile wheatgerm was added (1% w/w) and mixed well. Approximately 8g of soil was placed into each Petri dish to cover the Pythium lawn.
  • strain NCIB 40189 inhibited fungal growth in the soil. Its activity is comparable to Metalaxyl, a standard chemical fungicide.
  • strain NCIB 40189 The effectiveness of Pseudomonas fluorescens strain NCIB 40189 was further tested against Pythium ultimum and Rhizoctonia solani. Results show that strain NCIB 40189 has activity against P ultimum and R solani under glasshouse conditions.
  • Tests were carried out in pots (3 inches diameter), filled to 3/4 with infested soil. Five peas were sown in each pot and covered with clean soil.
  • strain NCIB 40189 results for strain NCIB 40189 and for the untreated control show that application of the Pseudomonas fluorescens strain allows significantly more pea plants to emerge. Activity of strain NCIB 40189 is comparable with that of the chemical fungicide Metalaxyl at the disease levels expressed.
  • Tests were carried out in pots (3 inches diameter), filled to 3/4 with infested soil. Five peas were sown in each pot and covered with clean soil.
  • HEALTHY CONTROL 1/10 TSB alone on soil not infested with Rhizoctonia. Five replicates were prepared for each treatment.
  • strain NCIB 40189 allows significantly more pea plants to emerge when compared to the diseased control. In fact, application of strain NCIB 40189 allows pea establishment at a level comparable to the healthy control (treatment without R solani) . Activity of the BCA also appears to be higher than that of the chemical fungicide Monceren.
  • Pseudomonas fluorescens strain NCIB 40189 was further tested for its activity against Rhizoctonia solani and its ability to protect pea plants from disease.
  • the trial was carried out in rectangular trays (165mm x 95mm), half-filled with infested soil (prepared according to the method described in the previous section). Twenty-five peas were sown in each tray and covered with clean soil.
  • strain NCIB 40189 significantly increased the percentage emergence of the plants when compared to the untreated control. There is no statistically significant difference between results obtained with the drench or granule BCA treatments.
  • strain NCIB 40189 was used in field trials as a biological control agent (BCA) against "damping-off" disease in peas and maize. Field trials of strain NCIB 40189 have been conducted on its potential for inhibiting Pythium and Fusarium spp. Results were compared to standard chemical treatments, and are reported below. In summary, these results show that disease caused by Pythium ultimum or Fusarium solani is reduced by application of strain NCIB 40189 to a level comparable with that obtained by standard chemical treatment. EFFICACY AGAINST PYTHIUM:
  • Strain NCIB 40189 was applied as a gum/peat formulation (10 cell/seed).
  • APRON Trade Mark
  • Two types of plant control were used: untreated pea seed, and blank pea seed (which had undergone seed treatment without addition of BCA or chemical).
  • the first column in Table 6 shows results for the control experiment where tests were conducted in normal field soil, without any added pathogen. The absence of pathogen in the soil allowed normal plant growth. There is no real significant difference between treatments, indicating the addition of strain NCIB 40189 had no phytotoxic effect.
  • the other figures in Table 6 give the results for two test treatments at different pathogen rates. The presence of pathogen in the soil decreased the number of plants emerging from untreated or blank seed when compared to the control experiment. This shows disease was present and was adversely affecting plant growth. However, the presence of strain NCIB 40189 significantly increases the number of plants emerging in the presence of the pathogen. EFFICACY AGAINST FUSARIUM:
  • Pathogen _ solani
  • Strain NCIB 40189 was applied as a gum/peat formulation (10 cell/seed).
  • the chemical control was CAPTAN (Trade Mark), used as a seed treatment at the recommended rate. Untreated seed was used as the plant control.
  • Results are shown in Table 7 below. Emergence is quoted as percentage (%) of plants 15 days after sowing. The letters appearing after the percentage emerged indicate statistical significance. There is no significant difference at the 5% probability level between entries with a letter in common.
  • the first column of Table 7 shows results for the control trials where no test pathogen was added.
  • the absence of pathogen in the soil allowed normal plant growth.
  • the presence of pathogen, Fusarium solani, in the soil decreased the number of plants emerging from untreated seed when compared to the control experiment. This shows disease was present and was adversely affecting plant growth.
  • the presence of strain NCIB 40189 significantly increases the percentage of plants emerging in the presence of the pathogen.
  • treatment with strain NCIB 40189 improved plant performance so that the percentage emerging was not significantly different from the percentage emerging in the absence of pathogen.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

On a isolé une nouvelle souche de Pseudomonas fluorescens qui présente une activité fongicide sur un large éventail de mycoses. On fournit les résultants de criblage, d'analyses et d'essais pratiques. L'agent fongicide est particulièrement actif contre la maladie de fonte des semis provoquée par des champignons de genres tels que Rhizoctonia, Pythium, Fusarium. On décrit des méthodes d'utilisation de tels micro-organismes et de compositions fongicides contenant ceux-ci pour protéger des plantes agricoles des mycoses. L'agent antifongique peut être utilisé comme fongicide agricole, en particulier comme enrobage de semences.
EP92907473A 1991-04-11 1992-04-03 Micro-organismes antifongiques Withdrawn EP0579635A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9107678 1991-04-11
GB919107678A GB9107678D0 (en) 1991-04-11 1991-04-11 Antifungal micro-organism

Publications (1)

Publication Number Publication Date
EP0579635A1 true EP0579635A1 (fr) 1994-01-26

Family

ID=10693067

Family Applications (1)

Application Number Title Priority Date Filing Date
EP92907473A Withdrawn EP0579635A1 (fr) 1991-04-11 1992-04-03 Micro-organismes antifongiques

Country Status (13)

Country Link
EP (1) EP0579635A1 (fr)
JP (1) JPH06506345A (fr)
AU (1) AU656369B2 (fr)
BG (1) BG61654B1 (fr)
CA (1) CA2107098A1 (fr)
CZ (1) CZ280653B6 (fr)
FI (1) FI934441A0 (fr)
GB (1) GB9107678D0 (fr)
HU (1) HUT67770A (fr)
NO (1) NO933623D0 (fr)
NZ (1) NZ242291A (fr)
RU (1) RU2104645C1 (fr)
WO (1) WO1992018613A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9207352D0 (en) * 1992-04-03 1992-05-13 Ici Plc Method to control fungal disease
US5496547A (en) * 1994-01-24 1996-03-05 Ciba-Geigy Corporation Pseudomonas biocontrol strains
FI95598C (fi) * 1994-01-31 1996-02-26 Kemira Agro Oy Mikro-organismi kasvitautien biologiseen torjuntaan
FI97148C (fi) * 1994-07-14 1996-10-25 Lahden Polttimo Ab Oy Menetelmä kasvien käsittelemiseksi siementen laatuominaisuuksien parantamiseksi
ES2186963T3 (es) * 1998-12-24 2003-05-16 Gabriele Dr Berg Aislados de rizobacterias para su utilizacion contra hongos del suelo fitopatogenos y procedimiento para la utilizacion de aislados de rizobacterias.
RU2170510C2 (ru) * 1999-07-01 2001-07-20 Совхоз "Тепличный" Способ получения биологического препарата "экофит" для защиты растений от фитопатогенов и повышения урожая
BR0009629A (pt) 2000-12-08 2002-08-20 Embrapa - Empresa Brasileira De Pesquisa Agropecua Biocontrole de doenças de plantas causadas por espécies de fusarium com novos isolados de bacillus megaterium e pantoea agglomerans
HU230555B1 (hu) * 2001-08-13 2016-12-28 Biofil Kft. Környezetbarát mikroorganizmus-készítmény és annak előállítása
RU2205542C1 (ru) * 2001-09-26 2003-06-10 Всероссийский научно-исследовательский институт биологической защиты растений Способ обработки растений и используемая в нем композиция для защиты растений
RU2305927C2 (ru) * 2005-06-09 2007-09-20 Федеральное государственное научное учреждение "Всероссийский научно-исследовательский институт защиты растений" Способ защиты растений
EP2236037A1 (fr) * 2009-03-30 2010-10-06 Technische Universität Graz Préparation fongicide
MD508Z (ro) * 2012-01-06 2012-12-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Procedeu de tratare a seminţelor de viţă-de-vie înainte de semănat
BR122022010122B1 (pt) * 2014-01-31 2023-05-16 Agbiome, Inc Composição, uso da composição, método para controlar um patógeno vegetal, formulação para o controle de um patógeno vegetal, uso da formulação, método para cultivar uma planta e uso de um agente de controle biológico nrrl n° b-50897
US9877486B2 (en) 2014-01-31 2018-01-30 AgBiome, Inc. Methods of growing plants using modified biological control agents

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI821099A0 (fi) * 1982-03-30 1982-03-30 Risto Tahvonen Fungistatiskt foerfarande
US4456684A (en) * 1982-09-08 1984-06-26 The United States Of America As Represented By The Secretary Of Agriculture Method for screening bacteria and application thereof for field control of diseases caused by Gaeumannomyces graminis
US4647533A (en) * 1984-09-14 1987-03-03 The United States Of America As Represented By The Secretary Of Agriculture Method for screening bacteria and application thereof for field control of Pythium spp. on small grain crops
IL87322A0 (en) * 1988-08-02 1989-01-31 Univ Ramot Method of controlling foliar diseases caused by fungal pathogens with fungicidal bacteria and novel pure cultures of fungicidal bacteria
US4996049A (en) * 1988-12-19 1991-02-26 Pioneer Hi-Bred International, Inc. Biological control of corn seed rot and seedling blight

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9218613A1 *

Also Published As

Publication number Publication date
NZ242291A (en) 1993-08-26
JPH06506345A (ja) 1994-07-21
CZ210693A3 (en) 1994-04-13
WO1992018613A1 (fr) 1992-10-29
HUT67770A (en) 1995-04-28
BG61654B1 (bg) 1998-02-27
AU1531592A (en) 1992-11-17
AU656369B2 (en) 1995-02-02
RU2104645C1 (ru) 1998-02-20
BG98144A (bg) 1994-06-30
GB9107678D0 (en) 1991-05-29
FI934441L (fi) 1993-10-08
NO933623L (no) 1993-10-08
FI934441A7 (fi) 1993-10-08
HU9302694D0 (en) 1994-01-28
CZ280653B6 (cs) 1996-03-13
CA2107098A1 (fr) 1992-10-12
NO933623D0 (no) 1993-10-08
FI934441A0 (fi) 1993-10-08

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