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EP0571741A2 - Plasmide pour l'expression dans des graines - Google Patents

Plasmide pour l'expression dans des graines Download PDF

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Publication number
EP0571741A2
EP0571741A2 EP93105138A EP93105138A EP0571741A2 EP 0571741 A2 EP0571741 A2 EP 0571741A2 EP 93105138 A EP93105138 A EP 93105138A EP 93105138 A EP93105138 A EP 93105138A EP 0571741 A2 EP0571741 A2 EP 0571741A2
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EP
European Patent Office
Prior art keywords
plasmid
expression plasmid
gene
expression
promoter
Prior art date
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Ceased
Application number
EP93105138A
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German (de)
English (en)
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EP0571741A3 (fr
Inventor
Asako Iida
Akitsu Nagasawa
Kenji Oheda
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Sumitomo Chemical Co Ltd
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Sumitomo Chemical Co Ltd
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Publication date
Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Publication of EP0571741A2 publication Critical patent/EP0571741A2/fr
Publication of EP0571741A3 publication Critical patent/EP0571741A3/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Definitions

  • the present invention relates to an expression plasmid for seeds. More particularly, the present invention is concerned with an expression plasmid constructed from a promoter and a GY terminator of a soybean seed storage protein glycinin gene (hereinafter abbreviated to "GY").
  • GY soybean seed storage protein glycinin gene
  • Glycinin is one of the main storage proteins occupying 20% or more of the whole seed protein of the soybean and is classified ,for the most part, into five types. Glycinin genes are designated as GY1, GY2, GY3, GY4 and GY5 respectively, and properties thereof are described in The Plant Cell , Vol. 1, pp. 313-328 (1989).
  • the present inventors have made an extensive study with the view to develop an excellent expression plasmid for seeds and, as a result, have found that an expression plasmid constructed from a promoter and a terminator obtained from certain storage protein genes in seeds has a higher expression ability than the conventional expression plasmid, which has led to the completion of the present invention.
  • an expression plasmid comprising a GY promoter and a GY terminator.
  • an expression plasmid comprising the GY promoter and the GY terminator and a plurality of cloning sites carried in a region downstream of the promoter.
  • a plant cell or whole plant comprising said expression plasmid.
  • the expression plasmid of the present invention can serve to enhance the expression of plant genes in seeds and is useful for plant breeding and for the production of valuable substances through the introduction and expression of foreign genes.
  • an expression plasmid contains a promoter sequence that is bonded with a RNA polymerase, and initiates the transcription.
  • the expression plasmid of the present invention contains a GY promoter as a promoter.
  • GY promoter include a known gene comprising 690 base pairs described for instance in Nucleic Acids Research , Vol. 17, 4386 (1989) and is represented by the nucleotide sequence of SEQ ID No. 3 of the attached "SEQUENCE LISTING" and a novel gene comprising 1116 base pairs represented by the nucleotide sequence of SEQ ID No. 1.
  • the expression plasmid further comprises a terminator sequence at the 3' terminus to stop the transcription.
  • the promoter plays a more important role than the terminator as regards the expression of valuable genes.
  • known terminators for example, nopaline synthase (NOS) may be used, independent of whether the gene has the same origin as the promoter or belongs to the same origin as the promoter from the viewpoint of taxonomy (for example, plants, animals, bacteria, yeast, fungi and virus).
  • NOS nopaline synthase
  • the expression plasmid of the present invention contains, as a terminator, a gene belonging to the same origin as the promoter, that is, a GY terminator.
  • GY terminator examples include a novel gene comprising 744 base pairs represented by the nucleotide sequence of SEQ ID No. 2 of the attached "SEQUENCE LISTING".
  • the terminator described herein is particularly useful for expression in the seeds of plants.
  • derived from a soybean seed storage protein glycinin gene refers to promoter or terminator sequences of a soybean seed storage protein glycinin gene comprising nucleotide substitutions, deletions or additions with respect to the wild type sequences without altering the functional activity of the elements as determined in expression tests.
  • the expression plasmid of the present invention further carries, in a region downstream of the promoter, at least one, preferably two or more, cloning sites into which a given foreign gene can easily be inserted.
  • cloning site used herein is intended to mean a site that can be recognized and cleaved with restriction enzymes used in conventional gene manipulations.
  • cloning site which can be carried in the expression plasmid of the present invention, include a gene represented by the nucleotide sequence of SEQ ID No. 4 in the attached "SEQUENCE LISTING".
  • expression plasmid carrying such a cloning site is pSUM-GY1 shown in Fig. 1.
  • Examples of useful foreign genes that can be introduced into plants with the aid of the expression plasmid according to the present invention include (plant) storage protein genes, such as glycinin genes, ⁇ -conglycinin genes and other genes of soybean, which can improve the protein content of seeds when expressed in seeds, (plant) protein genes, such as 2S-albumin genes of Brazil nut, 10 kDa or 15 kDa protein genes of corn, 10 kDa protein genes of rice and 10 kDa protein genes of sunflower and other genes, which can improve the seeds so as to have a higher methionine content or a higher lysine content when expressed in seeds, biotin-biosynthesis-related enzyme genes, such as bio A, bio B, bio C, bio D, bio F, bio H, and a transcription regulatory factor, birA, of microorganisms including Escherichia coli , or genes encoding their homologous enzymes of plants, which can improve the seeds so as to have a higher biotin content when expressed in seeds, and
  • a selectable marker for example, a drug-resistant gene, such as kanamycin or hygromycin, is added to the expression plasmid of the present invention capable of expressing the above-described valuable foreign genes, and the plasmid is then introduced into cells of dicotyledonous plants and monocotyledonous plants, such as soybean, tobacco , Arabidopsis , rice plant, corn, wheat, rapeseed and alfalfa, for example, by conventional direct gene transfer methods, such as a particle bombardment method, a polyethylene glycol (hereinafter referred to as "PEG") method wherein the plasmid DNA is incorporated into a protoplast by PEG, an electroporation method wherein the plasmid DNA is incorporated into a protoplast by applying an electric pulse and a microinjection method wherein the plasmid DNA is injected into plant cells with a micropipette, or a method wherein the expression plasmid of the present invention is once integrated within a T-DNA
  • the amount of expression plasmid of the present invention that is introduced into the plant cells is preferably about 1 to 10 ng per cell for the particle bombardment method, wherein use is made of a compressed-air-driver particle bombardment device (available from Rehbock Shoko Co., Ltd.), about 10 to 100 pg for the PEG method, about 10 to 100 pg per cell for the electroporation method, wherein an electric pulse of about 0.5 to 1 kV/cm is applied, and about 1 pg per cell for the microinjection method.
  • a compressed-air-driver particle bombardment device available from Rehbock Shoko Co., Ltd.
  • the plant cells, into which the expression plasmid of the present invention has been introduced, are cultured in a medium containing a drug as a selectable marker for the expression plasmid and a phytohormone, such as auxin, for about 1 to 2 months and further cultured in a regeneration medium for about 2 to 3 months to regenerate the whole plant.
  • a drug as a selectable marker for the expression plasmid and a phytohormone, such as auxin
  • the expression plasmid of the present invention can contribute to an improvement in the content of useful components in seeds, an improvement in the composition of the useful components and an improvement in the characteristic trait of plant seeds and enables useful substances to be produced in plant seeds by growing transformed plants wherein a foreign gene has been introduced and expressed.
  • Expression plasmid pSUM-GY1 comprising a promoter region and a terminator region isolated from GY1 and ligated respectively to both sides of a cloning site of plasmid pUC19 was constructed as follows.
  • plasmid pGY1-#4 an about 700 bp gene fragment in a promoter region was amplified and isolated by a polymerase chain reaction (PCR) based on nucleotide sequence of GY1 described in Nucleic Acids Research , Vol. 17, No. 11, p. 4386 (1989).
  • a soybean genomic library (available from Clontech, Co. Ltd.) was subjected to screening by using this gene fragment as a probe, a gene fragment of about 4.4 kb considered to contain intact GY1, that is, GY1-#4, was subjected to subcloning, and the resultant plasmid was designated as "plasmid pGY1-#4".
  • a promoter region and a terminator region were then isolated from plasmid pGY1-#4 and ligated respectively to both sides of a cloning site of plasmid pUC19 so as to construct plasmid pSUM-GY1. This is summarized in Figs. 3 to 5.
  • Step 1 Construction of Plasmid pSUM-GY1-B Containing the GY1 Terminator
  • a reaction solution containing 1 ⁇ g of plasmid pGY1-#4 and, added thereto, 10 units of restriction enzymes Hind III and Sph I was allowed to react at 37°C for one hour, thereby digesting plasmid pGY1-#4.
  • the reaction mixture was then provided with 0.8% agarose gel containing 0.1 ⁇ g/ml ethidium bromide and subjected to electrophoresis. After the completion of electrophoresis, a gel portion corresponding to a 1.2 kb Hind III- Sph I DNA fragment was cut out using ultraviolet irradiation, and the DNA fragment was purified by using a centrifugal tube equipped with a DNA recovery filter (available from Takara Shuzo Co., Ltd.).
  • a reaction solution containing 1 ⁇ g of plasmid pUC19 and, added thereto, 10 units of restriction enzymes Hind III and Sph I was allowed to react at 37°C for one hour.
  • 0.2 ⁇ g of the Hind III- Sph I DNA fragment of plasmid pUC19 and 0.2 ⁇ g of the 1.2 kb Hind III- Sph I DNA fragment of plasmid pGY1-#4 were mixed, and 5 units of T4 DNA ligase was added thereto so as to prepare a reaction solution that was then allowed to react at 16°C for 2 hr to effect ligation. Thereafter, according to a method established by Cohen et al. (see Proc. Natl. Acad. Sci.
  • the reaction mixture was subjected to agarose gel electrophoresis so as to select a plasmid cleaved at its one site with restriction enzyme NheI.
  • This plasmid was designated as "plasmid pSUM-GY1-A”.
  • a reaction solution containing 1 ⁇ g of plasmid pSUM-GY1-A and, added thereto, 10 units of restriction enzymes Sph I and Nhe I was allowed to react at 37°C for one hour.
  • DNA was purified by precipitation with ethanol, and about 0.1 ⁇ g of the purified DNA at its end was blunted with T4 DNA polymerase and then subjected to ligation with T4 ligase by using DNA Blunting Kit available from Takara Shuzo Co., Ltd. Thereafter, according to the method established by Cohen et al., the reaction mixture was transformed into E. coli HB101 strains, and an ampicillin-resistant colony was isolated. A plasmid was then prepared from the colony according to the method established by Birnboim et al. A reaction solution containing 1 ⁇ g of the plasmid and, added thereto, 5 units of restriction enzymes Sph I or Nhe I was allowed to react at 37°C for one hour.
  • the reaction mixture was subjected to agarose gel electrophoresis so as to select a plasmid cleaved at its one site with restriction enzyme Nhe I but not cleaved with restriction enzyme Sph I.
  • This plasmid was designated as "plasmid pSUM-GY1-B".
  • the plasmid pSUM-GY1-B had a cloning site derived from plasmid pUC19 and a GY1 terminator of about 0.7 kb.
  • Step 2 Preparation of a DNA fragment containing the GY1 Promoter
  • a reaction solution containing 1 ⁇ g of plasmid pGY1-#4 and, added thereto, 10 units of restriction enzymes Nco I and Sma I was allowed to react at 37°C for one hour.
  • the DNA was purified by precipitation with ethanol, and about 0.1 ⁇ g of the purified DNA at its end was blunted with T4 DNA polymerase and subjected to ligation with T4 ligase.
  • the reaction mixture was transformed into E.coli HB101 strains, and an ampicillin-resistant colony was isolated.
  • a plasmid was then prepared from the colony according to the method established by Birnboim et al.
  • a reaction solution containing 1 ⁇ g of the plasmid and, added thereto, 5 units of restriction enzymes Nco I or Sma I was allowed to react at 37°C for one hour.
  • the reaction mixture was subjected to agarose gel electrophoresis so as to select a plasmid cleaved at its one site with restriction enzyme Nco I but not cleaved with restriction enzyme Sma I.
  • This plasmid was designated as "plasmid pSUM-GY1-C”.
  • a reaction solution containing 1 ⁇ g of plasmid pGpSUM-GY1-C and, added thereto, 10 units of restriction enzymes Eco RI and Kpn I was allowed to react at 37°C for one hour.
  • the reaction mixture was subjected to electrophoresis, and a gel portion corresponding to a 1.1 kb Eco RI- Kpn I DNA was cut out, and the DNA fragment was purified by using a centrifugal tube equipped with a DNA recovery filter (available from Takara Shuzo Co., Ltd.).
  • Step 3 Construction of Plasmid pSUM-GY1
  • a reaction solution containing plasmid pSUM-GY1-B prepared in Step 1 and, added thereto, 10 units of restriction enzymes Eco RI and Kpn I was allowed to react at 37°C for one hour.
  • 0.2 ⁇ g of the Eco RI- Kpn I DNA fragment of plasmid pSUM-GY1-B and 0.2 ⁇ g of the approximately 1.1 kb Eco RI- Kpn I DNA fragment containing the GY1 promoter were mixed, and 5 units of T4 DNA ligase was added thereto to prepare a reaction solution that was then allowed to react at 16°C for 2 hr so as to effect ligation. Thereafter, according to the method established by Cohen et al., the reaction mixture was transformed into E.
  • plasmid was then prepared from the colony according to the method established by Birnboim et al. A reaction mixture containing 1 ⁇ g of the plasmid and, added thereto, 5 units of restriction enzymes Eco RI and Hind III was allowed to react at 37°C for one hour. The reaction mixture was subjected to agarose gel electrophoresis to select a plasmid from which two DNA fragments, that is, an approximately 1.8 kb DNA fragment and an approximately 2.7 kb DNA fragment, were detected. This plasmid was designated as plasmid "pSUM-GY1".
  • the constructed expression plasmid pSUM-GY1 was forwarded as Escherichia coli HB101/pSUM-GY1 on March 26, 1992 (Registered Mail Receipt No. 161 38 785294) to the Patent Microorganism Depository, Fermentation Research Institute, Agency of Industrial Science and Technology, and deposited as FERM BP-4131 under Budapest Treaty with said Patent Microorganism Depository (address: 1-3, Higashi 1 chome, Tsukuba-shi, Ibaraki-ken, 305, Japan) as the International Depository Authority.
  • a GUS gene as a reporter gene was inserted into a cloning site of the constructed expression plasmid pSUM-GY1, and the expression of the gene was examined in an immature seed of a soybean so as to determine the GY1 promoter/terminator activity. The method will now be described in detail.
  • a 1.87 kb GUS gene fragment derived from plasmid pRAJ225 (available from Clontech Co. Ltd.) was inserted into between Nco I and Nhe I within the multi-cloning site of plasmid pSUM-GY1 so as to construct the GUS expression plasmid pSUM-GY-001.
  • Plasmid pSUM-GY1-001 was introduced into an immature seed of a soybean 1 to 2 months after flowering together within an internal standard plasmid pD0432 (see Science , 234 , 856-859 (1986)) comprising luciferase (LUC) gene of a firefly ligated downstream of a cauliflower mosaic virus (CaMV) 35S promoter by a particle bombardment method (see Shokubutsu Saibo Kogaku , 2 , pp. 631-637 (1990)).
  • LOC luciferase
  • GUS expression plasmid pGY1100 having the same structure as plasmid-pSUM-GY1-001, except for the replacement of the GY1 terminator with a nopaline synthase gene (NOS) terminator, was introduced in the same manner as that described above. 24 to 48 hrs after the introduction of the plasmid, the GUS/LUC activity was determined according to a method established by Morikawa et al. (see Appl. Microbiol. Biotechnol ., 31 , pp. 320-322 (1989)). The results are given in Table 1.
  • the GUS/LUC activity was about three times that for the introduction of plasmid pGY1100. This demonstrates that the GY1 promoter/terminator has an activity in the tissue of an immature seed and, in the seed tissue, the novel GY1 terminator provides a higher expression ability than a known NOS terminator widely used in conventional plants.

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EP93105138A 1992-03-27 1993-03-29 Plasmide pour l'expression dans des graines. Ceased EP0571741A3 (fr)

Applications Claiming Priority (2)

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JP7064792 1992-03-27
JP70647/92 1992-03-27

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EP0571741A2 true EP0571741A2 (fr) 1993-12-01
EP0571741A3 EP0571741A3 (fr) 1995-04-19

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Cited By (44)

* Cited by examiner, † Cited by third party
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WO1998003655A3 (fr) * 1996-07-23 1998-03-26 Sapporo Breweries Adn regulateur de l'expression genique, cassette d'expression, vecteur d'expression et plante transgenique
WO1999024573A3 (fr) * 1997-11-12 1999-07-15 Du Pont Homologue du soja correspondant a l'activateur transcriptionnel, specifique des semences, de phaseolus vulgaris
EP0976832A3 (fr) * 1998-07-15 2000-06-07 Sumitomo Chemical Company, Limited Promoteur fonctionel dans une cellule vegetale
WO2000040710A3 (fr) * 1999-01-08 2000-09-28 Pioneer Hi Bred Int Promoteur de l'expression privilegiee dans une graine
WO2002016604A1 (fr) * 2000-08-22 2002-02-28 National Institute Of Agrobiological Sciences Procede d'accumulation de produit genique etranger a forte concentration dans des semences vegetales
EP1048733A3 (fr) * 1999-04-30 2002-07-31 Sumitomo Chemical Company, Limited Promoteurs végétaux
WO2004076617A2 (fr) 2003-02-27 2004-09-10 Basf Plant Science Gmbh Procede de production d'acides gras polyinsatures
WO2004092398A2 (fr) 2003-04-15 2004-10-28 Basf Plant Science Gmbh Sequence d'acides nucleiques codant pour des proteines associees a la reponse de stress abiotique et cellules vegetales et plantes a tolerance accrue au stress environnemental
WO2005083053A2 (fr) 2004-02-27 2005-09-09 Basf Plant Science Gmbh Procede pour produire des acides gras $g(v)-3 insatures dans des organismes transgeniques
WO2006069610A2 (fr) 2004-07-02 2006-07-06 Metanomics Gmbh Procede de production de produits chimiques fins
EP1539970A4 (fr) * 2002-08-01 2006-08-30 Exelixis Plant Sciences Inc Sequences de promoteurs associes a des semences
WO2006100241A2 (fr) 2005-03-22 2006-09-28 Basf Plant Science Gmbh Procede de production d'acides gras c20 et c22 polyinsatures avec au moins quatre liaisons doubles dans des plantes transgeniques
WO2007042510A2 (fr) 2005-10-13 2007-04-19 Basf Plant Science Gmbh Procede de production d'acide arachidonique et/ou d'acide eicosapentaenoique
WO2007087815A2 (fr) 2004-12-17 2007-08-09 Metanomics Gmbh Procédé de contrôle de production de produits chimiques fins
WO2007137973A2 (fr) 2006-05-31 2007-12-06 Metanomics Gmbh Manipulation du métabolisme de l'azote
WO2007141189A2 (fr) 2006-06-08 2007-12-13 Basf Plant Science Gmbh végétaux aux caractéristiques de croissance améliorées et procédé d'obtention
EP2045327A2 (fr) 2005-03-08 2009-04-08 BASF Plant Science GmbH Expression à amélioration de séquences d'intron
EP2053057A2 (fr) 2004-09-24 2009-04-29 BASF Plant Science GmbH Séquences d'acide nucléique codant pour des protéines associées à la réponse au stress abiotique et cellules végétales et plantes dotées d'une tolérance accrue au stress environnemental
EP2090662A2 (fr) 2006-04-05 2009-08-19 Metanomics GmbH Procédé de production d'un produit chimique fin
EP2145960A1 (fr) 2005-08-12 2010-01-20 BASF Plant Science GmbH Protéines de codage de séquences d'acide nucléique associées à une réponse de stress abiotique et cellules de plantes avec une augmentation de la tolérance au stress environnemental
EP2154252A1 (fr) 2008-07-16 2010-02-17 Sumitomo Chemical Company, Limited Procédés de production de protéines secrétées
EP2163630A1 (fr) 2008-09-03 2010-03-17 Sumitomo Chemical Co., Ltd. Procédé de contrôle du temps de floraison d'une plante
EP2166090A2 (fr) 2003-08-01 2010-03-24 BASF Plant Science GmbH Procédé de fabrication d'acides gras insaturés de manière multiple dans des organismes transgéniques
EP2177605A1 (fr) 2006-10-06 2010-04-21 BASF Plant Science GmbH Procédé de production d'acides gras polyinstaurés dans des organimes transgéniques non-humains
DE112008001453T5 (de) 2007-05-22 2010-04-29 Basf Plant Science Gmbh Pflanzenzellen und Pflanzen mit erhöhter Toleranz und/oder Resistenz gegenüber Umweltstress und erhöhter Biomasseproduktion-KO
EP2189533A1 (fr) 2006-08-02 2010-05-26 CropDesign N.V. Installations dotées de caractéristiques de rendement améliorées et procédé de fabrication de celles-ci
EP2194140A2 (fr) 2005-03-02 2010-06-09 Metanomics GmbH Procédé de production de produits chimiques fins
DE112008003433T5 (de) 2007-12-21 2010-11-04 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag (KO NUE)
EP2333078A2 (fr) 2006-03-24 2011-06-15 BASF Plant Science GmbH Protéines associées à une réponse au stress abiotique et homologues
EP2307547A4 (fr) * 2008-06-28 2011-06-22 Donald Danforth Plant Sci Ct Production et stockage améliorés de protéines dans des plantes
EP2365063A1 (fr) 2003-03-31 2011-09-14 University Of Bristol Nouvelles acyltransférases végétales spécifiques aux acides gras insaturés multiples à chaîne longue
EP2366781A1 (fr) 2005-11-24 2011-09-21 BASF Plant Science GmbH Procédé de production d'acides gras non saturés delta 5 dans des organismes transgéniques
EP2377937A1 (fr) 2006-05-30 2011-10-19 CropDesign N.V. Plantes ayant une expression réduite d'un gen CLE-like, caractéristiques de rendement améliorées et procédé de fabrication de celles-ci
EP2380984A2 (fr) 2006-02-16 2011-10-26 BASF Plant Science GmbH Acide nucléique
EP2431472A1 (fr) 2005-07-06 2012-03-21 CropDesign N.V. Amélioration de rendement de plantes par expression génique de type STE20
EP2434019A1 (fr) 2003-08-01 2012-03-28 BASF Plant Science GmbH Procédé de production de produits chimiques fins
EP2439280A1 (fr) 2006-03-31 2012-04-11 BASF Plant Science GmbH Installations dotées de caractéristiques de rendement améliorées et procédé de fabrication de celles-ci
EP2471930A1 (fr) 2002-12-20 2012-07-04 Metanomics GmbH & Co. KGaA Procédé de production d'aminoacides
EP2492345A2 (fr) 2007-05-04 2012-08-29 BASF Plant Science GmbH Amélioration de semences par des combinaisons de pyruvate kinase
DE112009003708T5 (de) 2008-12-12 2012-09-13 Basf Plant Science Gmbh Desaturasen und Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenenOrganismen
EP2573188A2 (fr) 2005-03-02 2013-03-27 Metanomics GmbH Procédé de production de produits chimiques fins
EP2573179A1 (fr) 2005-09-15 2013-03-27 CropDesign N.V. Augmentation du rendement végétal par l'expression de groupe 3 lea
EP2623584A1 (fr) 2004-02-27 2013-08-07 BASF Plant Science GmbH Procédé de fabrication de plusieurs acides gras insaturés dans des plantes transgéniques
WO2015092709A1 (fr) 2013-12-17 2015-06-25 Basf Plant Science Company Gmbh Procédés de conversion de la spécificité de substrat de désaturases

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TR27832A (tr) * 1987-04-29 1995-08-31 Monsanto Co Zararli ucucu hasarata mukavim bitkiler.
JPH062064B2 (ja) * 1987-06-24 1994-01-12 農林水産省食品総合研究所長 新規なｄｎａ鎖
AU7583691A (en) * 1990-03-05 1991-10-10 Upjohn Company, The Protein expression via seed specific regulatory sequences

Cited By (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955649A (en) * 1996-07-23 1999-09-21 Sapporo Breweries Ltd. Gene expression regulatory DNA, expression cassette, expression vector and transgenic plant
WO1998003655A3 (fr) * 1996-07-23 1998-03-26 Sapporo Breweries Adn regulateur de l'expression genique, cassette d'expression, vecteur d'expression et plante transgenique
WO1999024573A3 (fr) * 1997-11-12 1999-07-15 Du Pont Homologue du soja correspondant a l'activateur transcriptionnel, specifique des semences, de phaseolus vulgaris
EP0976832A3 (fr) * 1998-07-15 2000-06-07 Sumitomo Chemical Company, Limited Promoteur fonctionel dans une cellule vegetale
US6218598B1 (en) 1998-07-15 2001-04-17 Sumitomo Chemical Company, Limited Plant promoter
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