EP0467987A4 - Methods and materials for expression of human plasminogen in a eukaryotic cell system - Google Patents

Methods and materials for expression of human plasminogen in a eukaryotic cell system

Info

Publication number: EP0467987A4
Authority: EP; European Patent Office
Prior art keywords: expression; materials; methods; cell system; eukaryotic cell
Prior art date: 1989-05-01
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP19900907737

Other languages

English (en)

Other versions

EP0467987A1 (fr

Inventor

Francis J. Castellino

Joann L. Whitefleet-Smith

Elliot D. Rosen

James H. Mclinden

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

University of Notre Dame

Original Assignee

University of Notre Dame

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-05-01

Filing date

1990-04-26

Publication date

1992-07-08

1990-04-26 Application filed by University of Notre Dame filed Critical University of Notre Dame

1992-01-29 Publication of EP0467987A1 publication Critical patent/EP0467987A1/fr

1992-07-08 Publication of EP0467987A4 publication Critical patent/EP0467987A4/en

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3153—Streptokinase
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Microbiology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
General Chemical & Material Sciences (AREA)
Immunology (AREA)
Virology (AREA)
Animal Behavior & Ethology (AREA)
Diabetes (AREA)
Hematology (AREA)
Chemical Kinetics & Catalysis (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Gastroenterology & Hepatology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Peptides Or Proteins (AREA)

EP19900907737 1989-05-01 1990-04-26 Methods and materials for expression of human plasminogen in a eukaryotic cell system Withdrawn EP0467987A4 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US34580189A	1989-05-01	1989-05-01
US345801		1989-05-01

Publications (2)

Publication Number	Publication Date
EP0467987A1 EP0467987A1 (fr)	1992-01-29
EP0467987A4 true EP0467987A4 (en)	1992-07-08

Family

ID=23356538

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP19900907737 Withdrawn EP0467987A4 (en)	1989-05-01	1990-04-26	Methods and materials for expression of human plasminogen in a eukaryotic cell system

Country Status (6)

Country	Link
EP (1)	EP0467987A4 (fr)
JP (1)	JPH05500748A (fr)
AU (1)	AU647391B2 (fr)
FI (1)	FI915149A7 (fr)
PT (1)	PT93933A (fr)
WO (1)	WO1990013640A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8927722D0 (en) *	1989-12-07	1990-02-07	British Bio Technology	Proteins and nucleic acids
US6743623B2 (en)	1991-09-27	2004-06-01	Centre National De La Recherche Scientifique	Viral recombinant vectors for expression in muscle cells
FR2681786A1 (fr) *	1991-09-27	1993-04-02	Centre Nat Rech Scient	Vecteurs recombinants d'origine virale, leur procede d'obtention et leur utilisation pour l'expression de polypeptides dans des cellules musculaires.
US6099831A (en) *	1992-09-25	2000-08-08	Centre National De La Recherche Scientifique	Viral recombinant vectors for expression in muscle cells
US5945403A (en)	1997-05-30	1999-08-31	The Children's Medical Center Corporation	Angiostatin fragments and method of use
US7420036B2 (en)	2000-11-28	2008-09-02	Waisman David M	Anti-angiogenic polypeptides
ES2296705T3 (es)	2000-12-21	2008-05-01	Thrombogenics N.V.	Vector de expresion de levadura y metodo de produccion de una proteina recombinada por la expresion de una celula de levadura.
US7067492B2 (en) *	2001-09-06	2006-06-27	Omnio Ab	Method of promoting healing of a tympanic membrane perforation
AU2003297181A1 (en)	2002-12-13	2004-07-09	Mediomics, Llc	Compositions and methods for inhibiting tumor growth and metastasis
US20120220015A1 (en) *	2009-05-26	2012-08-30	Biolex Therapeutics	Compositions and methods for production of aglycosylated plasminogen
AU2010270146B9 (en)	2009-07-10	2015-04-02	Thrombogenics Nv	Variants of plasminogen and plasmin
CN103384722B (zh)	2011-01-05	2016-11-16	斯路姆基因公司	纤溶酶原和纤溶酶变体
WO2013024074A1 (fr)	2011-08-12	2013-02-21	Thrombogenics N.V.	Variants du plasminogène et de la plasmine

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3943245A (en) *	1974-02-14	1976-03-09	Armour Pharmaceutical Company	Purification of plasminogen
EP0028489B1 (fr) *	1979-11-05	1983-10-05	Beecham Group Plc	Dérivés d'enzymes et leur préparation
US4769331A (en) *	1981-09-16	1988-09-06	University Patents, Inc.	Recombinant methods and materials
US4745051A (en) *	1983-05-27	1988-05-17	The Texas A&M University System	Method for producing a recombinant baculovirus expression vector

1990
- 1990-04-26 AU AU56596/90A patent/AU647391B2/en not_active Expired - Fee Related
- 1990-04-26 WO PCT/US1990/002296 patent/WO1990013640A1/fr not_active Ceased
- 1990-04-26 EP EP19900907737 patent/EP0467987A4/en not_active Withdrawn
- 1990-04-26 JP JP2507792A patent/JPH05500748A/ja active Pending
- 1990-04-26 FI FI915149A patent/FI915149A7/fi not_active Application Discontinuation
- 1990-04-30 PT PT93933A patent/PT93933A/pt not_active Application Discontinuation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 271, no. 2, June 1989, pages 390-399; J. WHITEFLEET-SMITH et al.: "Expression of human plasminogen cDNA in a baculovirus vector-infected insect cell system" *

Also Published As

Publication number	Publication date
EP0467987A1 (fr)	1992-01-29
AU5659690A (en)	1990-11-29
FI915149A0 (fi)	1991-10-31
PT93933A (pt)	1991-01-08
FI915149A7 (fi)	1991-10-31
JPH05500748A (ja)	1993-02-18
WO1990013640A1 (fr)	1990-11-15
AU647391B2 (en)	1994-03-24

Publication	Publication Date	Title
AU3212689A (en)	1989-10-05	Medical material permitting cells to enter thereinto and artificial skin
GB2216052B (en)	1992-05-27	Redrawing method and drawn-redrawn can
DK40591D0 (da)	1991-03-07	Tredimensionalt celle- og vaevskultursystem
EP0486290A3 (en)	1993-03-17	A method and composition for increasing the accumulation of squalene and specific sterols in yeast
ZA895973B (en)	1990-05-30	Preparation of ionic phosphites and their use in homogeneous transition metal catalyzed process
EP0335630A3 (en)	1990-03-14	Switching device and method of preparing it
GB9010561D0 (en)	1990-07-04	Enzymes and their use in drug detection
EP0467987A4 (en)	1992-07-08	Methods and materials for expression of human plasminogen in a eukaryotic cell system
AU6518390A (en)	1991-04-28	Femoro-patellar prosthesis and its devices for setting in place
EP0337300A3 (en)	1990-09-12	Apparatus and method for determining functions of cells
EP0367830A4 (en)	1993-04-07	Series control unit and method of control
GB8910693D0 (en)	1989-06-28	Improvements in and relating to safety devices and methods of using safety devices
EP0336661A3 (en)	1990-08-22	Wipes for use in a cleanroom and a method of making them
ZA937156B (en)	1994-05-23	Piperidine derivatives their preparation and theirapplication in therapy
AU3212589A (en)	1989-10-05	Medical material permitting cells to enter thereinto and artificial skin
PT83301B (en)	1988-10-06	Expression of superoxide dismutase in eukaryotic cells and process for obtaining the same
NZ227594A (en)	1991-09-25	Human interleukin-1alpha and preparations thereof
GB2240310B (en)	1993-11-24	Double acting switch and method of use
NZ293994A (en)	1999-02-25	Immortalized endothelial brain cell lines and their use in therapy
GB2225038B (en)	1992-08-12	Post support and method of making same
ZA86711B (en)	1986-09-24	Means and method of pneumatic comminution
AU1980288A (en)	1989-01-30	Improvements in and relating to decontamination enclosures
AU3971289A (en)	1990-02-05	Method and means of wrapping in particular infected waste
GB2219820B (en)	1992-05-27	Improvements in and relating to joint members
NO914269L (no)	1991-10-31	Fremgangsmaate og materialer for ekspresjon av humanplasminogen i et eukaryot cellesystem

Legal Events

Date	Code	Title	Description
1991-12-13	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
1992-01-29	17P	Request for examination filed	Effective date: 19911029
1992-01-29	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB IT LI LU NL SE
1992-07-08	A4	Supplementary search report drawn up and despatched	Effective date: 19920520
1992-07-08	AK	Designated contracting states	Kind code of ref document: A4 Designated state(s): AT BE CH DE DK ES FR GB IT LI LU NL SE
1994-05-18	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN
1994-06-29	18W	Application withdrawn	Withdrawal date: 19940322
1994-07-06	R18W	Application withdrawn (corrected)	Effective date: 19940322