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EP0456137A2 - Dispositif de pipettage - Google Patents

Dispositif de pipettage Download PDF

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Publication number
EP0456137A2
EP0456137A2 EP91107231A EP91107231A EP0456137A2 EP 0456137 A2 EP0456137 A2 EP 0456137A2 EP 91107231 A EP91107231 A EP 91107231A EP 91107231 A EP91107231 A EP 91107231A EP 0456137 A2 EP0456137 A2 EP 0456137A2
Authority
EP
European Patent Office
Prior art keywords
pipetting device
slide
housing
valve
hose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP91107231A
Other languages
German (de)
English (en)
Other versions
EP0456137A3 (en
Inventor
Adolf Martin Hirschmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glasgeratebau Hirschmann
Original Assignee
Glasgeratebau Hirschmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glasgeratebau Hirschmann filed Critical Glasgeratebau Hirschmann
Publication of EP0456137A2 publication Critical patent/EP0456137A2/fr
Publication of EP0456137A3 publication Critical patent/EP0456137A3/de
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries

Definitions

  • the invention relates to a pipetting device for aspirating liquids and / or filling vessels, preferably capillaries, with liquids by capillary action, and for blowing liquids out of vessels, preferably capillaries, according to the preamble of claim 1.
  • a pipetting device for drawing in and blowing out liquids from capillaries according to the preamble of claim 1 is known from DE-GM 88 14 099.7.
  • a pump which can deliver air in two directions, firstly in the suction direction and secondly in the blowout direction. Depending on the direction of conveyance, the air is sucked in or blown out. The delivery starts relatively suddenly when the pump is switched on, which can be disadvantageous in certain cases.
  • a precision valve for dosing devices for dosing the smallest amounts of liquid consists of a spring-loaded valve ball and a valve seat built into the valve housing.
  • the valve seat is formed by a ring made of an elastomeric material with a square or rectangular cross section or a cross section approximating this.
  • the US-PS 41 95 526 shows a pipetting device with a peristaltic pump.
  • a pipetting device which has a pipette holder and a suction line, a pressure line and a valve, which optionally creates a connection between the suction line, the pressure line and the pipette.
  • DE-GM 88 09 704 shows a pipetting device which consists of a housing with a handle and a pipette holder for a pipette and a vacuum pump with an electric motor for drawing in and expelling liquid. So that such a device can be used for pipettes of different sizes and so that a pipette can be filled quickly as well as precisely dosed, there are two voltage sources, which can be switched in parallel or in series by a switch.
  • the object of the invention is to simplify and improve a pipetting device of the type specified at the outset.
  • this object is achieved according to the characterizing part of claim 1 in that a valve is provided in the air duct which seals the air duct in the rest position and which can be opened.
  • the multi-way valve required in some previously known solutions can be dispensed with will.
  • the delivery be it in the suction direction or in the blow-out direction, begins suddenly when the pump is switched on. Rather, it is possible to switch on the pump before opening the valve and only then to open the valve slowly or faster. This enables the dosing to be carried out more precisely. If necessary, it is also possible to open the valve first and only then switch on the pump.
  • the valve consists of a ball, preferably a glass ball, which is inserted sealingly in a part of the air duct designed as a tube.
  • a ball valve has the advantage that it can be produced and metered very finely (precisely), that is, enables very precise metering.
  • a further advantageous further development is characterized by a hose receptacle which fits the part of the air duct formed as a hose on both sides of the ball valve and a slide which is mounted in a longitudinally displaceable manner in the housing with an operating part protruding from the housing and an actuating part which surrounds the part of the air duct designed as a hose in the region of the ball valve .
  • the ball valve is closed when the slide is at rest.
  • the ball preferably a glass ball, sits and seals in a part of the air duct designed as a tube.
  • the actuating part of the slide which surrounds the ball valve, moves with the slide.
  • the pipetting device is very simple.
  • the valve consists only of a hose holder, a slide and a ball arranged in the hose. Depending on the direction of movement (suction direction or blow-out direction) of the pump, liquid is sucked in or blown out.
  • the operation of the pipetting device according to the invention is very simple since only the slide has to be moved to open the valve.
  • the hose can be guided in an S-shape in the hose holder. This arrangement is particularly space-saving.
  • the hose receptacle is preferably U-shaped in cross section.
  • the actuating part of the slide preferably projects into the U-shaped cross section.
  • the hose receptacle preferably runs in the cross-sectional plane of the U-shaped cross section.
  • the slide can be guided in a longitudinally displaceable manner in guide recesses in the housing. This is particularly advantageous in the case of a two-part housing, since the guides can then be produced particularly easily as recesses in the housing halves.
  • the slide can have an operating part on each side of the housing. This makes the slide easy to use for both right-handed and left-handed users.
  • the slide is preferably designed to be symmetrical in itself and also symmetrical to the housing.
  • a further advantageous development is characterized in that a lever is provided which is pivotably mounted in the housing and which projects from one end of the housing and which is connected to the slider. The slide is then actuated by rotating the lever about its pivot point.
  • the lever is preferably connected to the slide between its pivot point and its end protruding from the housing. The lever is therefore designed as a one-armed lever.
  • the actuating part of the slide can enclose the ball valve by more than 180 °. If the actuating part surrounds the ball valve by 180 ° or a little more, it can be easily installed.
  • the slide is spring-loaded so that it automatically returns to its starting position after actuation.
  • the spring zero point preferably coincides with the neutral position of the slide, in which the ball valve is closed.
  • the spring preferably consists of a leaf spring connecting the housing to the slide.
  • This leaf spring can be made of plastic. It can be molded onto the slide. Furthermore, it can be inserted into corresponding recesses in the housing. This is also particularly advantageous if the housing consists of two housing halves. It is then possible to simply insert the spring into one of the housing halves or recesses provided therein and then to mount the second housing half.
  • the actuating part preferably encloses the hose with play.
  • the game can be about 2/10 mm.
  • the ball of the ball valve is forcibly centered.
  • the ball of the ball valve has more freedom of movement than within the actuating part of the slide due to the game mentioned within the adjacent hose parts, which are firmly fitted into the adjacent hose holder.
  • Another advantage of the game mentioned is that the slide must first cover a certain distance before opening the ball valve, which can be used to actuate the pump. This route can be used in particular to switch the pump on before the valve opens. For exact dosing, it is desirable to first switch on the pump and only then to open the valve.
  • the delivery be it in the suction direction or in the blow-out direction, would start suddenly, so that exact dosing would become more difficult or even impossible in certain applications.
  • the pump is switched on before the valve is opened. This can be done through the game mentioned. However, it is also possible that the pump is switched on first and then the valve is only opened in another way.
  • a further advantageous development is characterized by two switches which can be actuated by the slide, preferably microswitches, for switching on the pump in the suction and discharge directions.
  • One of the switches is used to switch on the pump in the suction direction, the other switch is used to switch on the pump in the discharge direction.
  • the switches can be actuated in such a way that the pump is first switched on before the valve is opened.
  • the switches and the play between the actuating part of the slide and the hose are coordinated with one another such that the pump is first switched on and only then is the valve opened.
  • the switches are preferably on different sides in the direction of movement of the slide arranged of the actuating part. Depending on the direction of movement of the slide, one of the two switches is operated.
  • One slide direction of movement is therefore used for suction, the other slide direction of movement serves to expel the liquid.
  • a further advantageous development is characterized by an ejector pin which can be moved in the direction of the capillary for removing the capillary from its receptacle and which can be actuated by a pusher.
  • the ejection pin preferably projects into the capillary receptacle. By pressing the pusher, the ejection pin is moved in the direction of the capillary, so that the capillary is moved out of its receptacle and ejected.
  • the air duct preferably runs through the ejection pin and / or the pusher.
  • the air duct can run partly in the longitudinal direction of the lever and partly transversely to the longitudinal direction of the lever.
  • the arrangement of part of the air duct in the ejector pin and / or in the pusher or in part of the pusher is particularly space-saving.
  • the ejector pin preferably has a cylindrical part on its side facing the pusher which can be inserted into a corresponding cylindrical bore in the pusher. This makes assembly easier. Furthermore, the ejector pin can be designed as an easily replaceable wear part.
  • a filter preferably a replaceable sterile filter
  • the filter can then be easily replaced.
  • a liquid filter can be used for this purpose. However, this must be replaced when it comes into contact with liquid.
  • the eject pin can be removed from the pusher.
  • the filter can then be removed from the eject pin and a new filter can be inserted. Finally, the unit consisting of the ejector pin and filter is reinserted into the handle.
  • the pusher is preferably spring-loaded so that it automatically returns to its starting position after actuation to eject the capillary.
  • a further advantageous development is characterized by a capillary channel branching off the air channel, in which a valve is provided and the end of which is connected to the ambient pressure.
  • a valve is provided and the end of which is connected to the ambient pressure.
  • the valve provided in the capillary channel is opened. Since the same ambient pressure prevails on both sides of the capillary, the capillary can be filled by capillary action.
  • the branch of the capillary channel from the air channel is preferably arranged in the pusher.
  • the valve in the capillary channel is preferably a ball valve.
  • a preferably spring-loaded actuation button for actuating the valve is mounted in the capillary channel in the housing. This button can be used to operate the valve in the capillary channel.
  • the pipetting device shown in FIGS. 1 and 2 for aspirating and / or filling vessels, preferably capillaries, and for blowing liquids out of vessels, preferably capillaries consists of a housing 1 with a capillary receptacle 13, 14, a vane pump 2 for conveying air in two directions, namely in the suction direction and in the blow-out direction, and an air duct from the capillary receptacle 13, 14 to the pump 2 with a valve, which consists of a glass ball 7, which seals into a part of the air duct designed as a tube is used.
  • the pipetting device can be used for pipetting glass micro precision capillaries.
  • the housing 1 is a pistol grip designed with finger depressions 21.
  • the air channel runs from the capillary receptacle 13 via a channel provided in the ejection pin 12 through the hydrophobic sterile filter 11 into a channel 22 which is provided within the pusher 10.
  • the channel 22 within the handle 10 extends partially in the longitudinal direction of the handle. At its end facing away from the ejection pin, the channel 22 then bends downwards at right angles.
  • the hose 9, which leads the air duct to the hose receptacle 24, is pushed over a shoulder 23 on the pusher 10. The other end of the hose 9 is connected to the vane pump 2.
  • the glass ball 7 is inserted sealingly into the tube 9. This glass ball 7 forms a ball valve that is normally closed.
  • the hose 9 is fitted on both sides of the ball valve 7 with a precise fit in the hose receptacle 24.
  • a slide 8 is mounted for longitudinal displacement. The possibility of moving the slide 8 is shown by the double arrow B.
  • the slider 8 has operating parts 25 protruding from the housing 1, which have an arc-shaped profile and which can be corrugated for better displaceability.
  • An operating part 25 is provided on each side of the housing 1.
  • the slide 8 is symmetrical both in itself and relative to the housing 1. At its lower end in FIGS. 1 and 2, the slide 8 has an actuating part 26 which surrounds the hose 9 in the region of the ball valve 7.
  • the hose 9 is guided in an S-shape in the hose receptacle 24.
  • the hose receptacle 24 is - as can be seen from FIG. 2 - U-shaped in cross section, the actuating part 26 of the slide 8 protruding into the opening of the U cross section.
  • the level of the S-shaped curvature of the hose 9 is the same level like the plane of the U-shaped cross section of the hose receptacle 24, namely the plane of the drawing in FIG. 2.
  • the actuating part 26 of the slide 8 surrounds the ball valve 7 by a little more than 180 °. As a result, the actuating part 26 can be easily assembled; it is simply pushed over the ball valve.
  • the actuating part 26 surrounds the hose 9 in the region of the ball valve 7 with play. In the example shown, this game is about 2/10 mm. As a result, the ball 7 is automatically centered. It has more space in the area of the actuating part 26 than in the areas of the hose receptacle 24 adjacent to the left and right (see FIG. 2), so that it is inevitably held in the center of the actuating part 26 in the position shown in FIG. 2.
  • the slider 8 is guided in longitudinally displaceable manner in guide recesses 27 of the two-part housing 1.
  • the ends of the guide recesses 27 simultaneously form the stops for the slide 8.
  • the operating parts 25, which protrude from the housing 1, form the stops for the slide 8 with their ends.
  • the slide 8 is spring-loaded by a leaf spring 28.
  • the leaf spring 28 is made of plastic. It is molded onto the slide, in the area of its actuating part 26. Furthermore, the leaf spring 28 is inserted into corresponding recesses in the two housing halves 1.
  • the slide 8 can be used to actuate two microswitches 5, 6, which are arranged in the direction of movement B of the slide 8 on different sides of the actuating part 26 of the slide 8.
  • the actuating part 26 of the slide has this Purpose of an extended end portion 29 at the bottom left in Fig. 1, to which the switching lugs 30 of the microswitches 5, 6 are applied.
  • the electric motor 31 driving the vane pump 2 is powered by the battery 4, which can be charged by a charger via the socket 3.
  • both switches 5 and 6 are in the initial position, that is, unactuated.
  • the switch 6 is actuated, the positive pole of the battery 4 is applied to the negative pole of the motor 31 via the unchanged switch 5.
  • the negative pole of the battery 4 is applied to the positive pole of the electric motor 31 via the switch 6 that is switched on.
  • the electric motor then drives the vane pump in the blow-out direction.
  • the switch 5 When the switch 5 is actuated, the positive pole of the battery 4 is applied to the positive pole of the motor 31 via the unchanged switch 6. The negative pole of the battery 4 is applied to the negative pole of the electric motor 31 via the slide switch 16 and the switched switch 5. The vane pump is then operated in the suction direction.
  • the device can be switched on or off as a whole by the on / off slide switch 16. In the neutral switching position of the switches 5 and 6 shown in FIG. 3, both poles of the electric motor 31 rest on the positive pole of the battery 4, so that the motor does not move.
  • the ejection mechanism is located at the front end of the housing 1 shown in FIGS. 1 and 2 above.
  • a substantially cylindrical receptacle 32 is in one piece with the housing 1 connected to which the outer part 14 of the capillary receptacle is attached. Between the outer capillary holder 14 and the holder 32, the inner capillary holder 13 is clamped with an outer collar thereof.
  • the capillary is located in the capillary receptacles 13 and 14. It is held in a sealed manner in the inner capillary receptacle 13.
  • the pusher 10 In the receptacle 32, the pusher 10 is guided so as to be longitudinally displaceable.
  • An ejector pin 12 is inserted into the end of the pusher 10 facing the capillary receptacle 13.
  • the ejection pin 12 projects partially into the passage opening of the capillary receptacle 13.
  • the middle part of the ejector pin 12 is approximately the same diameter as the end part of the pusher 10.
  • the ejector pin 12 can be moved in the direction of the capillary, so that the capillary can be moved out of its receptacle 13 by this movement.
  • the ejector pin 12 is moved by a corresponding movement of the pusher 10.
  • the pusher 10 can be actuated simply by pressing on the end shown on the right in FIG. 1. Then the handle moves back to the starting position by the spring 15, which is supported on the one hand on the housing 1 and on the other hand on a plate which is snapped into a groove of the handle.
  • the ejection pin 12 has on its side facing the handle 10 a cylindrical part which can be inserted into a corresponding cylindrical bore in the handle.
  • a hydrophobic sterile filter 11 is located in this cylindrical part of the ejection pin 12.
  • the unit consisting of the pusher 10 and the ejection pin 12 is removed from the device.
  • the ejector pin 12 can then be separated from the pusher 10. This can be done by pulling the protruding end of the ejector pin 12 out with pliers becomes.
  • the filter 11 that is now accessible can then be replaced. Then everything is reassembled in reverse order.
  • the hypophobic sterile filter 11 serves to prevent liquids from penetrating into the device.
  • the pipetting device works as follows: The switch 16 is closed for the suction movement, so that the device as a whole is switched on (switch position of the switch 16 as shown in FIG. 3). By pulling the slide 8, that is to say moving the slide backwards (downward in FIG. 1), the pump 2 is put into suction operation via the microswitch 5. By pulling the slide 8 further, the ball valve 7 is opened so that the suction process is initiated and thus liquid is sucked into the capillary. When the slider 8 is released, it returns to the starting position due to the action of the spring 28.
  • the blow-out movement is generated by pushing the slide 8, that is to say moving the slide 8 upward in FIG. 1.
  • the pump 2 is set in pressure movement via the microswitch 6.
  • the ball valve 7 is opened, which initiates the blow-out process via the silicone hose 9 of the trigger system 10, 12.
  • the liquid is thus blown out of the capillary.
  • spring action spring 28
  • the pusher 10 By actuating the pusher 10, the ejection movement of the capillary is initiated. After reaching the stop (in the exemplary embodiment shown: 6 mm stroke), the capillaries have emerged from the sealing part 13; they therefore fall out of the capillary receptacle 14. By releasing the button 10 this returns again, brought about by the compression spring 15, into the starting position.
  • the energy part consists of a power supply via the battery 4 with 1.5 V and 600 mA. Recharging can take place via the charging socket 3.
  • the vane pump 2 is suitable for clockwise and anti-clockwise rotation, thus for suction and pressing.
  • the control takes place via the two microswitches 5 and 6 for right and left rotation of the vane pump 2.
  • a capillary duct 41 branching off the air duct is provided, in which the valve 17 is located.
  • the end of the capillary channel 41 is connected to the ambient pressure.
  • the branching of the capillary channel 41 is arranged in the pusher 10.
  • the pusher 10 has a further attachment 42, over which the tube 43 forming the capillary channel 41, which is only partially illustrated, is pushed.
  • the valve 17, which is located in the capillary channel 41, is a ball valve. It can be actuated by an actuation button 44 mounted in the housing 1.
  • the pusher 10 is pressed to eject the capillary.
  • the slide 8 is pulled so that the microswitch 5 is actuated and the pump 2 is actuated in the suction direction.
  • the capillary valve 17 is actuated by pressing the button 44, that is to say opened.
  • FIG. 6 shows a third embodiment, in which the same parts are again identified by the same reference numbers.
  • the slide 8 by a lever 51 operated.
  • the lever 51 is pivotally mounted at its end shown on the left in FIG. 6 about a pivot point provided in the housing.
  • the pivot point is essentially cylindrical; it is enclosed by the correspondingly cranked and screwed-in end of the lever 51.
  • the other end 52 of the lever 51 protrudes from the housing 1. It consists of a cap placed on the lever, which is movable and guided in a recess in the housing 1.
  • the lever 51 is connected to the slide 8 between its pivot point and its end 52 protruding from the housing 1.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Jet Pumps And Other Pumps (AREA)
EP19910107231 1990-05-07 1991-05-03 Pipetting apparatus Withdrawn EP0456137A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19904014588 DE4014588A1 (de) 1990-05-07 1990-05-07 Pipettiergeraet
DE4014588 1990-05-07

Publications (2)

Publication Number Publication Date
EP0456137A2 true EP0456137A2 (fr) 1991-11-13
EP0456137A3 EP0456137A3 (en) 1992-04-22

Family

ID=6405866

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19910107231 Withdrawn EP0456137A3 (en) 1990-05-07 1991-05-03 Pipetting apparatus

Country Status (2)

Country Link
EP (1) EP0456137A3 (fr)
DE (1) DE4014588A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2711075A1 (fr) * 1993-10-13 1995-04-21 Monostat Corp Appareil de pipetage automatique à main.
DE19717361A1 (de) * 1997-04-24 1998-10-29 Walu Labortechnik Gmbh Pipettiergerät
WO2004062805A1 (fr) * 2003-01-14 2004-07-29 Pz Htl Spólka Akcyjna Procede pour remplir de liquide un dispositif de pipetage et dispositif de pipetage

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5509318A (en) * 1993-10-13 1996-04-23 Manostat Corporation Memory Mopet
DE19742005A1 (de) * 1997-09-24 1999-04-01 Microdrop Ges Fuer Mikrodosier Vorrichtung und Verfahren zum genauen Dosieren und Umsetzen von kleinen Flüssigkeitsmengen
DE19917029C2 (de) * 1999-04-15 2001-08-09 Inst Mikrotechnik Mainz Gmbh Verfahren und Vorrichtung zur dosierten Ausgabe von Flüssigkeitsmengen im Bereich von 0,1 nl bis 100 mul

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3219417A (en) * 1960-08-25 1965-11-23 Franz Bergmann K G Pipetting device
US3310002A (en) * 1965-10-18 1967-03-21 Robbin Lab Inc Pipette pump
US3719087A (en) * 1966-04-08 1973-03-06 R Thiers Pipetting apparatus and method
AU451101B2 (en) * 1970-07-23 1974-07-05 Bioscience Laboratories Pipetting apparatus and method
US4195526A (en) * 1978-02-09 1980-04-01 Corning Glass Works Hand-held pipetter
US4257268A (en) * 1979-08-24 1981-03-24 Data Packaging Corporation Pipetter
DE8619168U1 (de) * 1986-07-17 1986-10-16 Witeg-Glasgeräte Helmut Antlinger KG, 6980 Wertheim Präzisionsventil
JPS6347665A (ja) * 1986-08-14 1988-02-29 コントロン　インスツルメンツ　ホールディング　エヌ．ブイ． ピペット操作方法および装置
DE8809704U1 (de) * 1988-07-29 1988-12-15 Glasgerätebau Hirschmann, 7101 Eberstadt Pipettiergerät
DE8814099U1 (de) * 1988-11-10 1989-02-09 Glasgerätebau Hirschmann, 7101 Eberstadt Pipettiergerät

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2711075A1 (fr) * 1993-10-13 1995-04-21 Monostat Corp Appareil de pipetage automatique à main.
DE19717361A1 (de) * 1997-04-24 1998-10-29 Walu Labortechnik Gmbh Pipettiergerät
DE19717361C2 (de) * 1997-04-24 1999-06-24 Walu Labortechnik Gmbh Pipettiergerät
DE19717361C3 (de) * 1997-04-24 2002-08-22 Walu Labortechnik Gmbh Pipettiergerät
WO2004062805A1 (fr) * 2003-01-14 2004-07-29 Pz Htl Spólka Akcyjna Procede pour remplir de liquide un dispositif de pipetage et dispositif de pipetage

Also Published As

Publication number Publication date
DE4014588C2 (fr) 1992-10-22
DE4014588A1 (de) 1991-11-14
EP0456137A3 (en) 1992-04-22

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