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EP0225922A1 - Recipient analytique - Google Patents

Recipient analytique

Info

Publication number
EP0225922A1
EP0225922A1 EP19860903980 EP86903980A EP0225922A1 EP 0225922 A1 EP0225922 A1 EP 0225922A1 EP 19860903980 EP19860903980 EP 19860903980 EP 86903980 A EP86903980 A EP 86903980A EP 0225922 A1 EP0225922 A1 EP 0225922A1
Authority
EP
European Patent Office
Prior art keywords
test chamber
housing
cavity
reaction
test chambers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19860903980
Other languages
German (de)
English (en)
Inventor
Francis K. Mundon
Sean P. O'neill
Daniel H. Zimmerman
Michael J. Fisher
Didicus I. De Souza
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alfa Wassermann Inc
Electro Nucleonics Inc
Original Assignee
Alfa Wassermann Inc
Electro Nucleonics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann Inc, Electro Nucleonics Inc filed Critical Alfa Wassermann Inc
Publication of EP0225922A1 publication Critical patent/EP0225922A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Definitions

  • This invention relates to test devices, and more particularly to a device for conducting a chemical and/or biological reaction.
  • the invention has particular application to biological agglutination reactions.
  • test device in which a chemical and/or biological reaction may be carried out under the total control of the user, so that reagents or test samples may be introduced into the device and a reaction allowed to proceed to completion, permitting immediate evaluation of the test, preferably by inspection.
  • reagents or test samples may be introduced into the device and a reaction allowed to proceed to completion, permitting immediate evaluation of the test, preferably by inspection.
  • it would be of great benefit to the physician or technician administering the test if a patient's serum could be easily and simply introduced into a test device for immediate reaction with reagents either already in the device or introduced therein at the time of the test, with ,the test results being immediately visualized for the purpose of diagnosis.
  • the present invention provides such a device, and in apresently preferred embodiment constitutes base and cover members that are joined together and which together define a plurality of sealed test chambers wherein reactions may be conducted.
  • one of the members is of flexible material which is releasably and sealingly joined to the other member to provide access to the test chambers for introduction of a patient's serum into those chambers.
  • Lens elements may be included on one of the members for imaging the test chambers.
  • the test chambers may be defined by generally conical cavities which enhance the sensitivity by providing wells for the collection of precipitates that are formed.
  • those test chambers may be deformed so that different portions thereof are imaged by the lens elements.
  • variations in focal length of the lens elements may be compensated for by selective deformation of the walls of the test chambers, as necessary.
  • One of the base and cover members advantageously is of thermoplastic rubber material which is inert to substances used in tests.
  • that material is colored white, which shows up the various colors of reaction products.
  • That member could be clear for light transmission, as desired, or a color could be selected for good reflection of light, typically provided by white coloration.
  • the thermoplastic rubber has an excellent gripping property, which is utilized for sealing purposes. Because of its flexibility, it may be easily removed to provide access to the test chambers.
  • Those test chambers advantageously may be conically shaped, as noted above, so that a precipitated reaction product tends to collect at the bottom of the chamber so that it may be readilyvisualized. To aid in visualization lens elements provided in the device image the bottoms of the conical wells and provide magnification, as desired.
  • Fig. 1 is a perspective view of a test device embodying the present invention in which a flexible base member is shown partially peeled away from an associated cover member to provide access to test chambers.
  • Fig. 2 is alongitudinal sectional view through a device of the type in Fig. 1.
  • Fig. 3 is a plan view of a base member forming a part of the device of Figs. 1 and 2.
  • Fig. 4 is a sectional view of the base member of Fig. 3, taken along the Section 4-4 in Fig. 3.
  • -4- Fig. 5 is a side view of the base member of Fig. 3.
  • Fig. 6 is a plan view of a cover member forming a part of the device of Figs. 1 and 2.
  • Fig. 7 is a longitudinal sectional view of the cover member of Fig. 6, taken along the Section 7-7 in Fig. 6.
  • a testing device 10 is shown defined by a base member 12 and a cover member 14.
  • the assembly of Fig. 1 is shown in longitudinal section; in assembled form, in Fig. 2.
  • the base member 12 is of flexible material (it is shown partially “peeled” away from the cover member 10 in Fig. 1) and is of thermoplastic rubber grade, advantageously either pure or oil-modified block copolymer rubbers, such as KRATON thermoplastic rubber grade produced by Shell Chemical Company.
  • thermoplastic rubber grade advantageously either pure or oil-modified block copolymer rubbers, such as KRATON thermoplastic rubber grade produced by Shell Chemical Company.
  • Such material is flexible and yet has excellent gripping properties so that it may form an effective seal in the completed device, which may be released as desired for the purpose of adding serum and reagents to test chambers, as will be described below.
  • Figs. 3 to 5 show the details of the base member 12. It may be of generally white-colored material, so that individual colors of precipitated components may be readily visualized.
  • the base member 12 includes a base section 16 from which a peripheral lip 18 depends from one side thereof ' around the periphery of the base member.
  • a plurality of cavities 20 are generally concial in configuration. Although reaction products may tend to accumulate along the sides of the conical cavity, they do tend to concentrate in the V-shaped lower portion 20a of the cavity for better visualization.
  • the cavities 20 terminate at the level of the base section 16 in a ledge 22 bounded by a cylindrical wall 24, which is beveled, as at
  • Figs. 6 and 7 show the cover member 14.
  • it is formed of transparent rigid plastic material, such as acrylic plastic, formed with a rim 26 extending about the periphery thereof on both sides of an intermediate web section 28.
  • Lens elements 30 depend from one side of the web section 28, while upstanding cylindrical wall members 32 extend from the other side of the base section 28..
  • the base member 12 and the cover member 14 are interfitted together as shown in Fig. 2.
  • the flexible rubber material of the base member releasably and- sealingly engages the cylindrical walls 32 of the cover member, as shown, so that sealed test chambers 34 are provided.
  • the device In use, the device is typically positioned on a surface, such as the top of a table, with the orientation as in Fig. 2, so that rim edge 26a is positioned on the table top.
  • the flexible base member 12 is peeled from the cover member 14, as shown in Fig. 1, so that reagents and serum samples, for example, may be inserted into the now- open test chambers 34.
  • the device is sealed by repositioning the flexible base member 12 to the configuration shown in Fig. 2, and then the device is aggitated and preferably inverted so that it rests on the table top, this time with the edgel 8 positioned on the table top, with the lens elements 30 being upwardly exposed.
  • reaction products proceed within the test chambers 34, and reaction products that precipitate out are collected in the conical cavities 20.
  • Precipitated reaction products may accumulate along the walls of the cavities, but accumulations will occur in the cavity bottom portions 20a, where they may be easily visualized, particularly with the aid of the lens elements 30.
  • the lens elements 30 are normally fabricated so that they image the lower portions 20a of the conical cavities. Any variations in focal length which may occur because of manufacturing tolerances may be compensated for by flexing the cavity walls 20, by hand. Furthermore, by sufficiently manipulating the flexible cavity walls, any desired portion of a cavity wall may be imaged by the corresponding lens element 30.
  • the complete unit may be sealed, if preferred, so that it is pre-packaged with reagents, if desired, or provision may be made for the insertion of reagents and sample serum into the various test chambers by penetration of the flexible base material 12 by syringe.
  • the base material 12 should be of self- sealing characteristic, so that the syringe penetration does not result in leakage from the test chambers.
  • the opitcal characteristics of the various parts of the device may vary, depending upon the usage desired.
  • the entire device may be transparent, so that it may be positioned within a spectrophotometer, for example, for optical automated testing.
  • the device will find applicaiton for visualization of the test results by a physician or a technician, who simply views the cavities through the lens elements following completion of a test procedure.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Dispositif (10) permettant d'exécuter des réactions chimiques et/ou biologiques, comprenant un organe de base (12) et un organe de couvercle (14) reliés entre eux et définissant ensemble des chambres de test étanches. L'un des organes (12) se compose d'un matériau flexible qui est raccordé de manière étanche et libérable à l'autre organe (14) afin de permettre l'accès aux chambres de test. Des éléments lenticulaires (30) sont compris dans l'un des organes (14) pour mettre en image les chambres de test. Les parois des chambres de test peuvent être réalisées en un matériau flexible de manière à pouvoir se déformer pour permettre la mise en image de différentes parties des chambres de test à l'aide des éléments lenticulaires (30). Une pluralité de cavités coniques (20) sont formées dans l'organe de base (12) de sorte que les produits de réaction s'accumulent dans les parties inférieures en V (20a) des cavités (20), assurant une meilleure visibilité.
EP19860903980 1985-06-07 1986-06-04 Recipient analytique Withdrawn EP0225922A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74234685A 1985-06-07 1985-06-07
US742346 2003-12-19

Publications (1)

Publication Number Publication Date
EP0225922A1 true EP0225922A1 (fr) 1987-06-24

Family

ID=24984464

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19860903980 Withdrawn EP0225922A1 (fr) 1985-06-07 1986-06-04 Recipient analytique

Country Status (3)

Country Link
EP (1) EP0225922A1 (fr)
AU (1) AU5991686A (fr)
WO (1) WO1986007288A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4832422A (en) * 1987-09-08 1989-05-23 Carter-Hoffman Corporation One-piece drawer with integral guide structure
US5214277A (en) * 1992-06-15 1993-05-25 Drennen Iii James K Near-infrared reflectance spectrometer system and related sample cell and sample support
DE59405534D1 (de) * 1993-07-02 1998-04-30 Inst Molekulare Biolog E V Probenträger und seine verwendung
US7070740B1 (en) * 2000-09-28 2006-07-04 Beckman Coulter, Inc. Method and apparatus for processing biomolecule arrays
US7169602B2 (en) 2002-12-04 2007-01-30 Applera Corporation Sample substrate for use in biological testing and method for filling a sample substrate
US7332348B2 (en) 2003-02-28 2008-02-19 Applera Corporation Sample substrate having a divided sample chamber and method of loading thereof
US20040258563A1 (en) 2003-06-23 2004-12-23 Applera Corporation Caps for sample wells and microcards for biological materials

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1196374A (en) * 1968-04-24 1970-06-24 Watson Marlow Instr Ltd Improvements in or relating to the Measurement of the Properties of Fluids
US4251159A (en) * 1979-01-15 1981-02-17 American Hospital Supply Corporation Disposable multi-chamber cuvette
US4391780A (en) * 1981-07-06 1983-07-05 Beckman Instruments, Inc. Container for sample testing
US4431307A (en) * 1981-11-19 1984-02-14 Labsystems Oy Set of cuvettes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO8607288A1 *

Also Published As

Publication number Publication date
AU5991686A (en) 1987-01-07
WO1986007288A1 (fr) 1986-12-18

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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18D Application deemed to be withdrawn

Effective date: 19870310

RIN1 Information on inventor provided before grant (corrected)

Inventor name: DE SOUZA, DIDICUS, I.

Inventor name: FISHER, MICHAEL, J.

Inventor name: ZIMMERMAN, DANIEL, H.

Inventor name: O'NEILL, SEAN, P.

Inventor name: MUNDON, FRANCIS, K.