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EP0137292B1 - Eprouvette pour analyses immunologiques - Google Patents

Eprouvette pour analyses immunologiques Download PDF

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Publication number
EP0137292B1
EP0137292B1 EP84110414A EP84110414A EP0137292B1 EP 0137292 B1 EP0137292 B1 EP 0137292B1 EP 84110414 A EP84110414 A EP 84110414A EP 84110414 A EP84110414 A EP 84110414A EP 0137292 B1 EP0137292 B1 EP 0137292B1
Authority
EP
European Patent Office
Prior art keywords
container
reaction container
wall
reaction
ribs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP84110414A
Other languages
German (de)
English (en)
Other versions
EP0137292A3 (en
EP0137292A2 (fr
Inventor
Pekka Sakari Peltonen
Pentti Juhani Juhala
Lasse Martti Hautoniemi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Farmos Yhtyma Oy
Original Assignee
Farmos Yhtyma Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Farmos Yhtyma Oy filed Critical Farmos Yhtyma Oy
Publication of EP0137292A2 publication Critical patent/EP0137292A2/fr
Publication of EP0137292A3 publication Critical patent/EP0137292A3/de
Application granted granted Critical
Publication of EP0137292B1 publication Critical patent/EP0137292B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se

Definitions

  • the invention relates to a tubular reaction container for immunological analyzes, which has at least at and next to its closed end an inner wall on which an antibody is fixed, to which the component to be analyzed (antigen) and a labeled component (labeled antigen) of a solution bind in the reaction vessel, the surface of the inner wall of the reaction vessel being enlarged at and near its closed end by the fact that inwardly directed formations are formed in the inner wall which are uniformly distributed over the circumference.
  • the formations are designed as flanges which are rectangular in cross section and rounded in side view and which practically only extend over the height of the round bottom of the reaction vessel.
  • the the reaction rate i.e. H. the diffusion distance from the liquid space to the wall, which determines the binding rate of the molecules on the wall, is relatively large.
  • the inner wall area is too small for some antibodies to be attached. Quite apart from this, it is disadvantageous that gussets are formed between the inner wall of the container and the flanges, which at least make emptying the reaction container more difficult and in which air bubbles can get caught.
  • the object of the invention is to improve the function of the generic reaction vessel.
  • the solution to this problem is that the formations consist of a maximum of eight round-flank ribs, which extend radially over a length of 1/4 to 1/2 the length of the container by 1/8 to slightly less than 1/2 of the inner diameter of the container extend inward into the container.
  • the diffusion distance is short and the inner surface available is more than sufficient in any case. Because the ribs are rounded, i.e. H. between rounded inner wall surfaces, complete emptying of liquid is guaranteed. That is e.g. B. when fixing the antibody on the inner wall of importance, with various chemical treatment and washing steps are required. In the case of immunological experiments, the reaction containers no longer need to be washed if there is complete emptying. This saves time and leads to more uniform test results. Due to the increased inner wall area, the outer diameter of the containers can be reduced, so that conventional analysis devices, which generally place limits on the outer diameter of the reaction containers, can be used. In any case, the reaction containers according to the invention deliver optimal results in practice.
  • the container is provided with four to six ribs arranged symmetrically.
  • Each rib can protrude from the wall into the reaction container by a piece, which corresponds to 1/5 to 1/3 of the inside diameter of the cross section of the container.
  • a particularly advantageous reaction container is a test tube, the lower or closed end of which is provided with five symmetrically arranged ribs on the tube wall, which extend from the wall into the tube by a piece corresponding to approximately 1/3 of the inner diameter of the tube cross section.
  • the reaction container according to the invention is preferably made of a plastic or another material containing hydrophilic functional groups, such as -COOH, -OH, -CHO, -NH 2 etc.
  • Suitable plastics for the reaction container are, for example, polyacrylate-ethylene copolymers; Polystyrene derivatives, such as polyaminostyrene, polystyrene acrylonitrile or polyvinylbenzyl chloride; Polyhydroxyethyl methacrylate.
  • reaction containers can perform immunological analyzes quickly and the possibility of errors decreases because the binding reaction can proceed almost to the end before the solution is removed from the container and the amount of labeled component bound to the wall is measured means a clear benefit to the user when hundreds of analyzes generally need to be performed daily.
  • the antibody is fixed to the reaction container in a simple and reliable manner, since the material of the reaction container contains functional groups.
  • the surface of the wall of the tubular reaction container is enlarged in that in the wall four ribs S are provided which extend over part of the length of the container.
  • the liquid-filled space V in the tube also becomes somewhat narrower.
  • the distance between two opposite ribs is about half the pipe diameter.
  • the liquid space V is thus divided into four compartments by the ribs S, which protrude into the container by about 1/3 of the diameter corresponding piece.
  • the reaction container according to the invention is used as follows: The test sample and the marked constituent are introduced into the reaction container. Thereupon the antigen in the sample and the labeled antigen, which functions as the labeled component, bind to the antibody on the wall of the reaction container. When the binding reaction is sufficiently advanced, the solution is poured from the reaction vessel and the amount of the labeled component adhered to the wall of the reaction vessel is measured. The amount of labeled component can also be measured from the solution poured out of the reaction container. The method of analysis depends on the labeled component used.
  • the antibody is fixed to the wall of the reaction container by chemical binding of the antibody to the functional groups of the material from which the reaction container is made.
  • reaction vessels are obtained, even when mass-produced, while also ensuring perfect storage.
  • the binding of the antigen and the labeled constituent to the antibody fixed to the wall of the reaction container takes place very quickly.
  • the reaction container according to the invention it is possible to analyze all such substances and combinations of substances which permit an immunological analysis.
  • the antibody fixed to the wall of the reaction container can also be an antigen.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices And Processes Conducted In The Presence Of Fluids And Solid Particles (AREA)

Claims (4)

1. Réacteur tubulaire pour analyses immunolo- giques, comprenant au moins sur et à côté de son extrémité fermée une paroi intérieure sur laquelle est fixé un anticorps auquel se lie le constituant à analyser et un constituant marqué d'une solution dans le réacteur, la surface de la paroi intérieure du réacteur à et au voisinage de son extrémité fermée étant agrandie par le fait que dans la paroi intérieure sont réalisées des excroissances réparties uniformément sur la circonférence et dirigées vers l'intérieur, caractérisé par le fait que les excroissances sont constituées au plus par huit nervures (S) à flanc arrondi qui, sur une longueur d'environ 1/4 à 1/2 de la longueur du réacteur, s'étendent radialement vers l'intérieur du réacteur sur 1/8 à un peu moins de 1/2 du diamètre intérieur.
2. Réacteur selon la revendication 1, caractérisé par le fait que le réacteur est muni de quatre à six nervures.
3. Réacteur selon la revendication 2, caractérisé par le fait que les nervures dépassent de 1/5 à 1/3 du diamètre intérieur du réacteur dans ledit réacteur.
4. Réacteur selon l'une des revendications 1 à 3, caractérisé par le fait qu'il est constitué d'une matière contenant des groupes hydrophiles fonctionnels.
EP84110414A 1983-09-08 1984-09-01 Eprouvette pour analyses immunologiques Expired - Lifetime EP0137292B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI833207 1983-09-08
FI833207A FI833207A0 (fi) 1983-09-08 1983-09-08 Reaktionskaerl foer immunologiska bestaemningar

Publications (3)

Publication Number Publication Date
EP0137292A2 EP0137292A2 (fr) 1985-04-17
EP0137292A3 EP0137292A3 (en) 1986-11-12
EP0137292B1 true EP0137292B1 (fr) 1990-03-28

Family

ID=8517705

Family Applications (1)

Application Number Title Priority Date Filing Date
EP84110414A Expired - Lifetime EP0137292B1 (fr) 1983-09-08 1984-09-01 Eprouvette pour analyses immunologiques

Country Status (6)

Country Link
EP (1) EP0137292B1 (fr)
JP (1) JPS60155972A (fr)
DE (1) DE3481760D1 (fr)
DK (1) DK157715C (fr)
ES (1) ES292536Y (fr)
FI (1) FI833207A0 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8394342B2 (en) 2008-07-21 2013-03-12 Becton, Dickinson And Company Density phase separation device
US8747781B2 (en) 2008-07-21 2014-06-10 Becton, Dickinson And Company Density phase separation device
US8794452B2 (en) 2009-05-15 2014-08-05 Becton, Dickinson And Company Density phase separation device
US9333445B2 (en) 2008-07-21 2016-05-10 Becton, Dickinson And Company Density phase separation device
US9682373B2 (en) 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4665035A (en) * 1986-05-27 1987-05-12 Josephino Tunac Fermentation apparatus and systems for the cultivation of microorganisms and other biological entities
DE4419971A1 (de) * 1994-06-08 1995-12-14 Eppendorf Geraetebau Netheler Gefäß zum Temperieren kleiner Flüssigkeitsmengen in einem Thermostaten
EP0959989B1 (fr) * 1997-02-14 2003-11-12 Dendreon Corporation Dispositif de lavage de cellules et procede associe
CA2356857A1 (fr) * 1998-12-24 2000-07-06 Sumitomo Bakelite Co., Ltd. Receptacle pour essais immunologiques
EP1234614A1 (fr) * 2001-02-27 2002-08-28 Pentapharm Gmbh Récipient-doseur subdivisé par des nervures pour la réception des réagents, sa fabrication et son usage

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE8331431U1 (de) * 1983-11-02 1984-02-23 Aktieselskabet NUNC, 4000 Roskilde Roehre fuer die immunologische adsorptionsanalyse

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1573224A (fr) * 1968-04-26 1969-07-04
US4111754A (en) * 1976-11-29 1978-09-05 Hydow Park Immunological testing devices and methods
US4146365A (en) * 1977-11-07 1979-03-27 Litton Bionetics, Inc. Affinity detection apparatus
FI58839C (fi) * 1979-04-30 1981-04-10 Orion Yhtymae Oy Saett att utfoera enzym-immunbestaemning och foer utfoerande av saettet avsett reagensroer
JPS5629168A (en) * 1979-08-16 1981-03-23 Toyobo Co Ltd Measuring method of body fluid component by immunity chemical reaction

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE8331431U1 (de) * 1983-11-02 1984-02-23 Aktieselskabet NUNC, 4000 Roskilde Roehre fuer die immunologische adsorptionsanalyse

Cited By (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9682373B2 (en) 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
US9714890B2 (en) 2008-07-21 2017-07-25 Becton, Dickinson And Company Density phase separation device
US8747781B2 (en) 2008-07-21 2014-06-10 Becton, Dickinson And Company Density phase separation device
US9933344B2 (en) 2008-07-21 2018-04-03 Becton, Dickinson And Company Density phase separation device
US8394342B2 (en) 2008-07-21 2013-03-12 Becton, Dickinson And Company Density phase separation device
US9333445B2 (en) 2008-07-21 2016-05-10 Becton, Dickinson And Company Density phase separation device
US9339741B2 (en) 2008-07-21 2016-05-17 Becton, Dickinson And Company Density phase separation device
US9700886B2 (en) 2008-07-21 2017-07-11 Becton, Dickinson And Company Density phase separation device
US9452427B2 (en) 2008-07-21 2016-09-27 Becton, Dickinson And Company Density phase separation device
US10807088B2 (en) 2009-05-15 2020-10-20 Becton, Dickinson And Company Density phase separation device
US8998000B2 (en) 2009-05-15 2015-04-07 Becton, Dickinson And Company Density phase separation device
US9364828B2 (en) 2009-05-15 2016-06-14 Becton, Dickinson And Company Density phase separation device
US9079123B2 (en) 2009-05-15 2015-07-14 Becton, Dickinson And Company Density phase separation device
US8794452B2 (en) 2009-05-15 2014-08-05 Becton, Dickinson And Company Density phase separation device
US9731290B2 (en) 2009-05-15 2017-08-15 Becton, Dickinson And Company Density phase separation device
US10343157B2 (en) 2009-05-15 2019-07-09 Becton, Dickinson And Company Density phase separation device
US9919308B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US9919309B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US12090476B2 (en) 2009-05-15 2024-09-17 Becton, Dickinson And Company Density phase separation device
US9919307B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US10376879B2 (en) 2009-05-15 2019-08-13 Becton, Dickinson And Company Density phase separation device
US10413898B2 (en) 2009-05-15 2019-09-17 Becton, Dickinson And Company Density phase separation device
US10456782B2 (en) 2009-05-15 2019-10-29 Becton, Dickinson And Company Density phase separation device
US9802189B2 (en) 2009-05-15 2017-10-31 Becton, Dickinson And Company Density phase separation device
US11351535B2 (en) 2009-05-15 2022-06-07 Becton, Dickinson And Company Density phase separation device
US11786895B2 (en) 2009-05-15 2023-10-17 Becton, Dickinson And Company Density phase separation device
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid

Also Published As

Publication number Publication date
DK157715C (da) 1990-07-09
DE3481760D1 (de) 1990-05-03
EP0137292A3 (en) 1986-11-12
DK429284A (da) 1985-03-09
ES292536Y (es) 1987-08-16
JPS60155972A (ja) 1985-08-16
EP0137292A2 (fr) 1985-04-17
ES292536U (es) 1987-01-01
FI833207A0 (fi) 1983-09-08
DK157715B (da) 1990-02-05
DK429284D0 (da) 1984-09-07

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