[go: up one dir, main page]

EA201791059A1 - Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений - Google Patents

Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений

Info

Publication number
EA201791059A1
EA201791059A1 EA201791059A EA201791059A EA201791059A1 EA 201791059 A1 EA201791059 A1 EA 201791059A1 EA 201791059 A EA201791059 A EA 201791059A EA 201791059 A EA201791059 A EA 201791059A EA 201791059 A1 EA201791059 A1 EA 201791059A1
Authority
EA
Eurasian Patent Office
Prior art keywords
plant
glycosylation
compositions
methods
plant cells
Prior art date
Application number
EA201791059A
Other languages
English (en)
Other versions
EA038931B1 (ru
EA038931B9 (ru
Inventor
Одед Шосейов
Хелена Магриссо
Цви Цвирин
Амит Яари
Original Assignee
Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд. filed Critical Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд.
Publication of EA201791059A1 publication Critical patent/EA201791059A1/ru
Publication of EA038931B1 publication Critical patent/EA038931B1/ru
Publication of EA038931B9 publication Critical patent/EA038931B9/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01051—Alpha-L-fucosidase (3.2.1.51)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Предложен способ модификации профиля гликозилирования полипептида, представляющего интерес, в растении или клетке растения. Указанный способ включает экспрессию в растении или клетке растения, трансформированных для экспрессии во внутриклеточном компартменте по меньшей мере одной гликозидазы, последовательности нуклеиновой кислоты, которая кодирует полипептид, представляющий интерес, таким образом, что по меньшей мере одна гликозидаза и полипептид, представляющий интерес, являются совместно локализованными во внутриклеточном компартменте растения или клетки растения, посредством чего модифицируется профиль гликозилирования полипептида, представляющего интерес, в растении или клетке растения.
EA201791059A 2014-11-20 2015-11-19 Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений EA038931B9 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462082204P 2014-11-20 2014-11-20
PCT/IL2015/051112 WO2016079739A2 (en) 2014-11-20 2015-11-19 Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells

Publications (3)

Publication Number Publication Date
EA201791059A1 true EA201791059A1 (ru) 2018-02-28
EA038931B1 EA038931B1 (ru) 2021-11-11
EA038931B9 EA038931B9 (ru) 2022-02-18

Family

ID=54851374

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201791059A EA038931B9 (ru) 2014-11-20 2015-11-19 Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений

Country Status (7)

Country Link
US (3) US20170283822A1 (ru)
EP (1) EP3221455B1 (ru)
CN (1) CN107810271B (ru)
BR (1) BR112017010555A2 (ru)
EA (1) EA038931B9 (ru)
IL (1) IL252389B (ru)
WO (1) WO2016079739A2 (ru)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3548612A4 (en) * 2016-12-01 2020-04-15 Plantform Corporation TRANSGENIC PLANT WITH REDUCED FUCOSYL TRANSFERASE AND XYLOSYL TRANSFERASE ACTIVITY
CN110092831B (zh) * 2018-01-30 2022-05-20 北京睿诚海汇健康科技有限公司 植物作为宿主在表达阿达木抗体中的应用
WO2019170899A1 (en) * 2018-03-08 2019-09-12 Targimmune Therapeutics Ag A vector for the expression of cetuximab antibody chains
JP7496347B2 (ja) 2018-08-17 2024-06-06 モダン メドウ，インコーポレイテッド アミノ酸をヒドロキシル化するための融合タンパク質及び生成物
KR102317403B1 (ko) * 2019-01-28 2021-10-29 주식회사 바이오앱 당화된 Ag85A 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법
WO2021101351A2 (ko) * 2019-11-21 2021-05-27 (주)지플러스 생명과학 비푸코실화된 담배를 이용하여 생산한 항체 및 이의 용도
US11326176B2 (en) 2019-11-22 2022-05-10 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
US12359212B2 (en) 2019-11-22 2025-07-15 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
KR102461600B1 (ko) * 2020-07-21 2022-11-02 주식회사 피토맵 N-당질화 돌연변이 벼, 이의 제조방법 및 이를 이용한 단백질 생산용 벼의 제조방법
KR20240066509A (ko) * 2022-11-02 2024-05-16 주식회사 피토맵 N-당질화 돌연변이 벼를 이용한 단일클론항체 생산용 벼의 제조방법 및 이에 의해 제조된 단일클론항체

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
CA1192510A (en) 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
JPS6054684A (ja) 1983-09-05 1985-03-29 Teijin Ltd 新規ｄνａ及びハイブリツドｄνａ
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
GB8608850D0 (en) 1986-04-11 1986-05-14 Diatech Ltd Packaging system
JPS6314693A (ja) 1986-07-04 1988-01-21 Sumitomo Chem Co Ltd 植物ウイルスｒｎａベクタ−
ES2060646T3 (es) 1987-02-09 1994-12-01 Lubrizol Genetics Inc Virus rna hibrido.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5360726A (en) 1989-09-12 1994-11-01 Board Of Trustees Operating Michigan State University Polypeptides enabling sorting of proteins to vacuoles in plants
US5202422A (en) * 1989-10-27 1993-04-13 The Scripps Research Institute Compositions containing plant-produced glycopolypeptide multimers, multimeric proteins and method of their use
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5849870A (en) 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
US5496934A (en) * 1993-04-14 1996-03-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acids encoding a cellulose binding domain
JP4052605B2 (ja) 1994-05-18 2008-02-27 タカラバイオ株式会社 α−１，３／４−フコシダーゼ遺伝子
WO1997000964A1 (en) 1995-06-23 1997-01-09 Danisco Ingredients A/S (Danisco A/S) Novel beta-xylosidase, nucleotide sequence encoding it, and use thereof
IL155588A0 (en) * 2003-04-27 2003-11-23 Metabogal Ltd Methods for expression of enzymatically active recombinant lysosomal enzymes in transgenic plant root cells and vectors used thereby
US20050032211A1 (en) 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
DE19900635A1 (de) * 1999-01-11 2000-07-13 Deutsches Krebsforsch Selektion von monoklonalen Antikörpern
US6331416B1 (en) * 1999-06-10 2001-12-18 Cbd Technologies Ltd. Process of expressing and isolating recombinant proteins and recombinant protein products from plants, plant derived tissues or cultured plant cells
WO2001029242A2 (en) 1999-10-21 2001-04-26 Monsanto Company Post-translational modification of recombinant proteins produced in plants
US20030159178A1 (en) 2000-02-10 2003-08-21 Peter Ulvskov Method for remodelling cell wall polysaccharide structures in plants
US6391683B1 (en) 2000-06-21 2002-05-21 Siliconware Precision Industries Co., Ltd. Flip-chip semiconductor package structure and process for fabricating the same
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US20090203079A1 (en) * 2000-10-20 2009-08-13 Board Of Trustees Of Michigan State University Transgenic monocot plants encoding beta-glucosidase and xylanase
JP4532118B2 (ja) * 2002-03-19 2010-08-25 スティヒティング ディーンスト ランドバウクンディフ オンデルズーク 植物体内のグリカンプロセシングの最適化
CL2003002461A1 (es) 2002-11-27 2005-01-07 Dow Chemical Company Agroscien Inmunoglobulina que comprende al menos un glicano afucosilado, composicion que la contiene, secuencia nucleotidica y vector que la comprende, procedimiento para producir dicha inmunoglobulina en plantas.
US7855063B2 (en) 2003-04-16 2010-12-21 Yeda Research And Development Co. Ltd. Partially deglycosylated glucocerebrosidase polypeptide and crystals thereof
US20050059053A1 (en) * 2003-07-21 2005-03-17 Rainer Fischer Complex formation for the stabilisation and purification of proteins of interest
JP5100386B2 (ja) 2004-09-29 2012-12-19 コルプラント リミテッド コラーゲン生産植物及びその作成方法及びその使用
CA2568633C (en) 2004-10-15 2008-04-01 Oren Halpern A system and a method for improving the captured images of digital still cameras
WO2007084926A2 (en) * 2006-01-17 2007-07-26 Biolex Therapeutics, Inc. Compositions and methods for humanization and optimization of n-glycans in plants
JP5568702B2 (ja) * 2006-11-08 2014-08-13 サントル ナショナル ドゥ ラ ルシェルシュ スィヤンティフィック（セーエヌエルエス） 組換えポリペプチドの発現および翻訳後修飾の制御を標的化するための配列を有するポリペプチド
WO2008128144A2 (en) * 2007-04-15 2008-10-23 Shuang Zhang Monoclonal antibody selecting system, and making and using thereof
EP3366762B1 (en) 2007-05-07 2020-07-08 Protalix Ltd. Large scale disposable bioreactor
BRPI0812538B1 (pt) * 2007-06-15 2021-03-16 Medicago Inc. processo para a síntese de uma proteína de interesse com fucosilação e xilosilação reduzidas, ácido nucleico, e proteína híbrida gnt1-galt
WO2009066303A2 (en) 2007-11-22 2009-05-28 Ganga Raju Gokaraju New synergistic phytochemical composition for the treatment of obesity
WO2009069123A2 (en) 2007-11-26 2009-06-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Compositions comprising fibrous polypeptides and polysaccharides
EP2090648A1 (en) * 2008-02-12 2009-08-19 Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) Production of glycosylated polypeptides in microalgae
US8815580B2 (en) * 2008-08-08 2014-08-26 Vib Vzw Cells producing glycoproteins having altered glycosylation patterns and method and use thereof
US8513397B2 (en) * 2008-08-27 2013-08-20 The Arizona Board Of Regents For And On Behalf Of Arizona State University DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
US20120079627A1 (en) 2009-05-29 2012-03-29 Edenspace Systems Corporation Plant gene regulatory elements
JP2013509180A (ja) * 2009-10-30 2013-03-14 メルク・シャープ・エンド・ドーム・コーポレイション 改善された分泌効率を有する組換えタンパク質の産生方法
US9249474B2 (en) * 2009-11-06 2016-02-02 Agrivida, Inc. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
US11673926B2 (en) * 2011-06-07 2023-06-13 Ibio, Inc. In vivo de-glycosylation of recombinant proteins by co-expression with PNGase F
US20140090108A1 (en) * 2012-09-18 2014-03-27 Freydoun Garabagi Vectors and Methods For Enhancing Recombinant Protein Expression in Plants
WO2014071039A1 (en) * 2012-11-01 2014-05-08 Cellectis Sa Plants for production of therapeutic proteins
TWI675044B (zh) * 2012-11-14 2019-10-21 美商再生元醫藥公司 重組細胞表面捕捉蛋白質
MX376838B (es) * 2013-03-15 2025-03-07 Univ Minnesota Ingenieria genomica de plantas utilizando sistemas crispr/cas.
US9689016B2 (en) * 2013-12-18 2017-06-27 Caliber Biotherapeutics, Llc Method for in vivo production of deglycosylated recombinant proteins used as substrate for downstream protein glycoremodeling

Also Published As

Publication number Publication date
US20230340516A1 (en) 2023-10-26
WO2016079739A2 (en) 2016-05-26
IL252389B (en) 2021-04-29
EP3221455B1 (en) 2020-08-12
CN107810271A (zh) 2018-03-16
CN107810271B (zh) 2021-09-28
US20190382781A1 (en) 2019-12-19
EA038931B1 (ru) 2021-11-11
US20170283822A1 (en) 2017-10-05
EP3221455A2 (en) 2017-09-27
WO2016079739A3 (en) 2016-07-14
IL252389A0 (en) 2017-07-31
WO2016079739A9 (en) 2017-02-09
BR112017010555A2 (pt) 2018-02-27
US11697819B2 (en) 2023-07-11
EA038931B9 (ru) 2022-02-18

Similar Documents

Publication Publication Date Title
EA201791059A1 (ru) Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений
CY1125979T1 (el) Εκφραση κατασκευασματων και μεθοδων εφαρμογης γενετικης μηχανικης σε μεθυλοτροφους ζυμομυκητες
MX2023011785A (es) Metodos y composiciones para produccion de proteina de clara de huevo.
EA202090931A2 (ru) Композиции и способы перепрограммирования tcr с помощью гибридных белков
MX2019005199A (es) Métodos para evaluar el riesgo usando and libre de células total y específico.
MX2017006802A (es) Animales no humanos que expresan el complejo cd3 humanizado.
EP4219744A3 (en) Methods of preparing nucleic acids for sequencing
CA2971734C (en) Anti-pd-1 antibodies
MX375524B (es) Anticuerpos anti-axl.
MX2017002765A (es) Il-23a y tnf-alfa orientados y compuesto y sus usos.
BR112016018105A2 (pt) célula eletroquímica, método de produção de uma célula eletroquímica
EA201990758A1 (ru) Способ получения электрокомпетентных дрожжевых клеток и способ применения указанных клеток
MX383142B (es) Composiciones que comprenden polipéptidos que tienen actividad xilanasa y polipéptidos que tienen actividad arabinofuranosidasa.
BR112017001550A2 (pt) enxerto de tecido
BR112016011004A2 (pt) animal roedor geneticamente modificado, célula tronco embrionária de roedor, embrião de roedor, e, métodos para fabricar um animal roedor e para enxertar células humanas em um camundongo
EP3577216A4 (en) EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS
MX390701B (es) Manufactura de proteína.
EA201791275A1 (ru) Способы получения ctp-модифицированных полипептидов длительного действия
MX2015014424A (es) Construcciones de proteinas mitocondriales y sus usos.
MX2020003508A (es) Un factor de secrecion de proteinas con alta eficacia secretora y un vector de expresion que lo comprende.
EA201691548A1 (ru) Экспрессирующие конструкции и способы для отбора клеток-хозяев, экспрессирующих полипептиды
AR100122A1 (es) Proteína antiportadora de protones / azúcar tonoplastidaria y su uso para aumentar la concentración de sacarosa de un órgano de almacenamiento de sacarosa de plantas
MX2020001223A (es) Proteinas de fusion tatk-cdkl5, composiciones, formulaciones y uso de estas.
EA201690831A1 (ru) Способы модулирования размера семян и органов у растений
BR112018005464A2 (pt) expressão de proteínas contendo fc

Legal Events

Date Code Title Description
TH4A Publication of the corrected specification to eurasian patent