EA200600585A1 - Система селекции, содержащая маркеры селекции, не связанные с устойчивостью к антибиотикам - Google Patents
Система селекции, содержащая маркеры селекции, не связанные с устойчивостью к антибиотикамInfo
- Publication number
- EA200600585A1 EA200600585A1 EA200600585A EA200600585A EA200600585A1 EA 200600585 A1 EA200600585 A1 EA 200600585A1 EA 200600585 A EA200600585 A EA 200600585A EA 200600585 A EA200600585 A EA 200600585A EA 200600585 A1 EA200600585 A1 EA 200600585A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- arad gene
- arad
- breeding
- gene
- selection
- Prior art date
Links
- 230000003115 biocidal effect Effects 0.000 title abstract 2
- 238000009395 breeding Methods 0.000 title 2
- 230000001488 breeding effect Effects 0.000 title 2
- 101150017736 araD gene Proteins 0.000 abstract 6
- 239000003550 marker Substances 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 108090000416 L-ribulose-5-phosphate 4-epimerases Proteins 0.000 abstract 1
- 238000009825 accumulation Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000001937 non-anti-biotic effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 229940124597 therapeutic agent Drugs 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/53—DNA (RNA) vaccination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Система селекции, лишенная генов устойчивости к антибиотикам, которая основана на применении гена araD в качестве маркера селекции, который несет вектор, встроенный в бактериальный штамм, дефицитный по гену araD. Ген araD из E.coli кодирует L-рибулозо-5-фосфат-4-эпимеразу. Способ селекции клеток, трансформированных плазмидой, которая содержит ген araD. Не связанный с антибиотиками маркер селекции делает данную систему подходящей для продукции терапевтических средств. Ген araD не существенен для роста хозяев, но манипуляция с ним влияет на рост в некоторых селективных условиях. Делеция araD ведет к накоплению вещества, токсичного для хозяина, но не для людей. Ген araD относительно мал, и поэтому может конструироваться малая плазмида, которая требует меньше энергии для репликации, и приводит к повышенным скорости роста и выходу.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20031319A FI116068B (fi) | 2003-09-15 | 2003-09-15 | Uusi selektiojärjestelmä, siinä käyttökelpoinen vektori, bakteerisolukantoja sekä menetelmä solujen valikoimiseksi |
| PCT/FI2004/000540 WO2005026364A1 (en) | 2003-09-15 | 2004-09-15 | Selection system containing non-antibiotic resistance selection marker |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA200600585A1 true EA200600585A1 (ru) | 2006-08-25 |
| EA011823B1 EA011823B1 (ru) | 2009-06-30 |
Family
ID=27838984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200600585A EA011823B1 (ru) | 2003-09-15 | 2004-09-15 | Бактериальная система селекции и ее применение |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US7521182B2 (ru) |
| EP (2) | EP1664305B1 (ru) |
| JP (1) | JP4732348B2 (ru) |
| KR (1) | KR101215245B1 (ru) |
| CN (1) | CN1882692B (ru) |
| AP (1) | AP1993A (ru) |
| AU (1) | AU2004272776B2 (ru) |
| BR (1) | BRPI0414392A (ru) |
| CA (1) | CA2538863C (ru) |
| DK (1) | DK1664305T3 (ru) |
| EA (1) | EA011823B1 (ru) |
| ES (1) | ES2529346T3 (ru) |
| FI (1) | FI116068B (ru) |
| HK (1) | HK1217111A1 (ru) |
| IL (1) | IL173923A0 (ru) |
| MX (1) | MXPA06002559A (ru) |
| NZ (1) | NZ545937A (ru) |
| PL (1) | PL1664305T3 (ru) |
| PT (1) | PT1664305E (ru) |
| SI (1) | SI1664305T1 (ru) |
| WO (1) | WO2005026364A1 (ru) |
| ZA (1) | ZA200602153B (ru) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2543079T3 (es) | 2004-08-13 | 2015-08-14 | The Trustees Of The University Of Pennsylvania | Métodos para construir vacunas sin resistencia antibiótica |
| US20060135457A1 (en) * | 2004-08-13 | 2006-06-22 | Yvonne Paterson | Methods for constructing antibiotic resistance free vaccines |
| JP5966018B2 (ja) * | 2011-12-21 | 2016-08-10 | バイオネット−エイジア，カンパニー・リミテッド | 改変ボルデテラ・パータシス株 |
| WO2014018602A1 (en) * | 2012-07-26 | 2014-01-30 | Glycos Biotechnologies, Inc. | Strains and methods for plasmid maintenance |
| EP2950816B1 (en) | 2013-02-04 | 2019-07-31 | Schweitzer Biotech Company Ltd. | The use of dna sequences encoding an interferon as vaccine adjuvants |
| JP2017512500A (ja) | 2014-03-25 | 2017-05-25 | ギンゴー バイオワークス， インコーポレイテッド | 細胞工学のための方法および遺伝システム |
| EP3569710A1 (en) * | 2018-05-16 | 2019-11-20 | BioVersys AG | Bacterial vectors for genetic manipulation of bacteria |
| CN109136247B (zh) * | 2018-08-09 | 2022-03-29 | 天津大学 | 一种用于铅结合蛋白定向进化的双向筛选系统构建方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843760A (en) | 1994-04-15 | 1998-12-01 | Midwest Research Institute | Single zymomonas mobilis strain for xylose and arabinose fermentation |
| KR20060099543A (ko) | 1994-12-09 | 2006-09-19 | 임페리얼 컬리지 이노베이션스 리미티드 | 유전자의 동정 |
| CA2219136A1 (en) | 1995-04-24 | 1996-10-31 | Chromaxome Corp. | Methods for generating and screening novel metabolic pathways |
| FR2738842B1 (fr) | 1995-09-15 | 1997-10-31 | Rhone Poulenc Rorer Sa | Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique |
| US6479279B2 (en) | 1995-12-29 | 2002-11-12 | Estonian Biocentre | Episomal vectors and uses thereof |
| US6162433A (en) | 1996-04-19 | 2000-12-19 | Medeva Europe Limited | Non antibiotic selectable markers for live vaccines |
| ES2157084T3 (es) | 1996-08-16 | 2001-08-01 | Medical Res Council | Vectores de expresion episomicos autorreplicantes que confieren expresion genica histoespecifica. |
| WO1998011231A1 (en) | 1996-09-10 | 1998-03-19 | The Rockefeller University | Highly regulable promoter for heterologous gene expression |
| KR100230578B1 (ko) | 1997-07-25 | 1999-12-01 | 허영섭 | 포스포리불로키나제를 융합파트너로 이용하는 재조합 인간 부갑상선호르몬의 발현벡터 |
| US6291245B1 (en) * | 1998-07-15 | 2001-09-18 | Roche Diagnostics Gmbh | Host-vector system |
| US6368793B1 (en) | 1998-10-14 | 2002-04-09 | Microgenomics, Inc. | Metabolic selection methods |
| DK1268823T3 (da) | 2000-03-24 | 2008-03-10 | Xoma Technology Ltd | Fremgangsmåder og celler til ekspression af rekombinante proteinprodukter |
| JP5016779B2 (ja) * | 2001-05-03 | 2012-09-05 | フィット バイオテク オーワイ | 発現ベクターおよびその使用方法 |
-
2003
- 2003-09-15 FI FI20031319A patent/FI116068B/fi not_active IP Right Cessation
-
2004
- 2004-09-15 JP JP2006526650A patent/JP4732348B2/ja not_active Expired - Fee Related
- 2004-09-15 DK DK04767054.2T patent/DK1664305T3/en active
- 2004-09-15 EA EA200600585A patent/EA011823B1/ru not_active IP Right Cessation
- 2004-09-15 ES ES04767054.2T patent/ES2529346T3/es not_active Expired - Lifetime
- 2004-09-15 PT PT47670542T patent/PT1664305E/pt unknown
- 2004-09-15 BR BRPI0414392-2A patent/BRPI0414392A/pt not_active Application Discontinuation
- 2004-09-15 WO PCT/FI2004/000540 patent/WO2005026364A1/en not_active Ceased
- 2004-09-15 EP EP04767054.2A patent/EP1664305B1/en not_active Expired - Lifetime
- 2004-09-15 PL PL04767054T patent/PL1664305T3/pl unknown
- 2004-09-15 AP AP2006003577A patent/AP1993A/xx active
- 2004-09-15 MX MXPA06002559A patent/MXPA06002559A/es active IP Right Grant
- 2004-09-15 EP EP14191756.7A patent/EP2949753A1/en not_active Withdrawn
- 2004-09-15 NZ NZ545937A patent/NZ545937A/xx not_active IP Right Cessation
- 2004-09-15 AU AU2004272776A patent/AU2004272776B2/en not_active Ceased
- 2004-09-15 US US10/531,870 patent/US7521182B2/en not_active Expired - Fee Related
- 2004-09-15 SI SI200432219T patent/SI1664305T1/sl unknown
- 2004-09-15 CA CA2538863A patent/CA2538863C/en not_active Expired - Fee Related
- 2004-09-15 CN CN2004800336943A patent/CN1882692B/zh not_active Expired - Fee Related
-
2006
- 2006-02-23 IL IL173923A patent/IL173923A0/en unknown
- 2006-03-14 ZA ZA200602153A patent/ZA200602153B/en unknown
- 2006-03-14 KR KR1020067005158A patent/KR101215245B1/ko not_active Expired - Fee Related
-
2016
- 2016-05-05 HK HK16105149.1A patent/HK1217111A1/en unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ KG MD TJ TM |
|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): BY KZ RU |