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EA199900668A1 - Способ идентификации рекомбинантной нуклеиновой кислоты (варианты), способ создания библиотеки экспрессируемых фрагментов генов (варианты), библиотеки экзогенных белков, нуклеиновых кислот, экспрессируемых фрагментов генов (варианты) - Google Patents

Способ идентификации рекомбинантной нуклеиновой кислоты (варианты), способ создания библиотеки экспрессируемых фрагментов генов (варианты), библиотеки экзогенных белков, нуклеиновых кислот, экспрессируемых фрагментов генов (варианты)

Info

Publication number
EA199900668A1
EA199900668A1 EA199900668A EA199900668A EA199900668A1 EA 199900668 A1 EA199900668 A1 EA 199900668A1 EA 199900668 A EA199900668 A EA 199900668A EA 199900668 A EA199900668 A EA 199900668A EA 199900668 A1 EA199900668 A1 EA 199900668A1
Authority
EA
Eurasian Patent Office
Prior art keywords
proteins
glycoproteins
rapid
novel
provides
Prior art date
Application number
EA199900668A
Other languages
English (en)
Inventor
Джеймс Э. Бейли
Вольфганг А. Реннер
Георг Х. Орбергер
Даниэль Коллер
Original Assignee
Сайтос Байотекнолэджи Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Сайтос Байотекнолэджи Гмбх filed Critical Сайтос Байотекнолэджи Гмбх
Publication of EA199900668A1 publication Critical patent/EA199900668A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/912—Absidia

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)

Abstract

Данное изобретение направлено на способ определения, получения характеристик и выделения нуклеиновых кислот, кодирующих белки с желательными свойствами, такими как локализация в клетке. Кроме того, изобретение представляет способ быстрой экспрессии данных белков или гликопептидов в клетках млекопитающих и облегчения очистки новых секретируемых белков или гликопротеинов. Далее изобретение представляет способ радиоактивного мечения новых белков или гликопротеинов с целью быстрой идентификации участков связывания, включая имеющиеся у животных, и быстрого получения инфекционных вирусных векторов для использования при переносе генов.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA199900668A 1997-11-17 1998-11-17 Способ идентификации рекомбинантной нуклеиновой кислоты (варианты), способ создания библиотеки экспрессируемых фрагментов генов (варианты), библиотеки экзогенных белков, нуклеиновых кислот, экспрессируемых фрагментов генов (варианты) EA199900668A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/972,218 US6197502B1 (en) 1997-11-17 1997-11-17 Expression cloning processes for the discovery characterization, and isolation of genes encoding polypeptides with a predetermined property
PCT/US1998/024520 WO1999025876A1 (en) 1997-11-17 1998-11-17 Expression cloning processes for the discovery, characterization and isolation of genes encoding polypeptides with a predetermined property

Publications (1)

Publication Number Publication Date
EA199900668A1 true EA199900668A1 (ru) 2000-06-26

Family

ID=25519360

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199900668A EA199900668A1 (ru) 1997-11-17 1998-11-17 Способ идентификации рекомбинантной нуклеиновой кислоты (варианты), способ создания библиотеки экспрессируемых фрагментов генов (варианты), библиотеки экзогенных белков, нуклеиновых кислот, экспрессируемых фрагментов генов (варианты)

Country Status (11)

Country Link
US (2) US6197502B1 (ru)
EP (1) EP0972086B1 (ru)
AT (1) ATE407219T1 (ru)
AU (1) AU760584B2 (ru)
CA (1) CA2282811A1 (ru)
DE (1) DE69839962D1 (ru)
EA (1) EA199900668A1 (ru)
IL (1) IL131759A0 (ru)
NO (1) NO994220L (ru)
NZ (1) NZ337297A (ru)
WO (1) WO1999025876A1 (ru)

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US6783939B2 (en) * 2000-07-07 2004-08-31 Alphavax, Inc. Alphavirus vectors and virosomes with modified HIV genes for use in vaccines
DE10040289A1 (de) * 2000-08-17 2002-02-28 Aventis Res & Tech Gmbh & Co Verfahren zur Herstellung und Ermittlung geeigneter Effektoren von Zielmolekülen mit Substanzbibliotheken
US6830885B1 (en) * 2000-08-18 2004-12-14 Phenogene Therapeutiques Inc. Nucleic acid molecule, method and kit for selecting a nucleic acid having a desired feature
AU2002254212A1 (en) * 2001-03-12 2002-09-24 Irm, Llc Identification of cellular targets for biologically active molecules
US20030014232A1 (en) * 2001-05-22 2003-01-16 Paterson Thomas S. Methods for predicting biological activities of cellular constituents
EP1432791B2 (en) * 2001-09-06 2013-10-23 Alphavax, Inc. Alphavirus replicon vector systems
US20040152105A1 (en) * 2002-09-06 2004-08-05 Cytos Biotechnology Ag. Immune modulatory compounds and methods
EP2290054B1 (en) * 2002-12-13 2017-03-29 Alphavax, Inc. Alphavirus particles and methods for preparation
EP1585812B1 (en) * 2002-12-13 2017-01-18 Alphavax, Inc. Multi-antigenic alphavirus replicon particles and methods
JP5016305B2 (ja) * 2003-03-20 2012-09-05 アルファヴァックス，インコーポレイテッド 改良されたアルファウイルスレプリコンおよびヘルパー構築物
ATE432285T1 (de) * 2003-07-11 2009-06-15 Alphavax Inc Cytomegalovirusimpfstoffe, die auf dem alphavirus basieren
CA2567254C (en) 2004-05-18 2012-03-13 Alphavax, Inc. Tc-83-derived alphavirus vectors, particles and methods
US7303898B2 (en) * 2005-03-29 2007-12-04 New York University Defective sindbis viral vectors
WO2007025008A2 (en) * 2005-08-24 2007-03-01 The Scripps Research Institute Translation enhancer-element dependent vector systems
PL2947149T3 (pl) 2007-06-21 2018-09-28 Alphavax, Inc. Kasety bez promotora do ekspresji alfawirusowych białek strukturalnych
US9234181B2 (en) * 2007-11-26 2016-01-12 Novartis Ag RNA expression cassette and cells for making alphavirus particles
EP2065398A1 (en) 2007-11-29 2009-06-03 Cytos Biotechnology AG Human monoclonal nicotine specific antibodies
AU2008329089A1 (en) 2007-11-29 2009-06-04 Cytos Biotechnology Ag Human monoclonal nicotine specific antibodies
WO2009075886A1 (en) 2007-12-11 2009-06-18 The Scripps Research Institute Compositions and methods related to mrna translational enhancer elements
WO2010005704A2 (en) * 2008-06-13 2010-01-14 New York University Novel helper plasmid, defective sindbis viral vectors and methods of use thereof
WO2010063675A1 (en) 2008-12-04 2010-06-10 Cytos Biotechnology Ag Fully human influenza m2 specific antibodies
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN107254488A (zh) * 2017-05-27 2017-10-17 上海市农业科学院 一种辛德毕斯病毒全长质粒型载体及其构建方法
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Also Published As

Publication number Publication date
DE69839962D1 (de) 2008-10-16
AU1415599A (en) 1999-06-07
NZ337297A (en) 2001-03-30
IL131759A0 (en) 2001-03-19
US6524792B1 (en) 2003-02-25
CA2282811A1 (en) 1999-05-27
NO994220L (no) 1999-09-27
AU760584B2 (en) 2003-05-15
WO1999025876A1 (en) 1999-05-27
EP0972086A4 (en) 2005-07-20
ATE407219T1 (de) 2008-09-15
EP0972086B1 (en) 2008-09-03
US6197502B1 (en) 2001-03-06
EP0972086A1 (en) 2000-01-19
NO994220D0 (no) 1999-08-31

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