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EA199800212A1 - METHODS OF OBTAINING SUSTAINABLE TRANSFORMABLE HIGH-PRODUCTIVE WHEAT BY TRANSFORMATION MEDIATED BY AGROBACTERIUM AND THE COMBINATION OBTAINED BY THEM - Google Patents

METHODS OF OBTAINING SUSTAINABLE TRANSFORMABLE HIGH-PRODUCTIVE WHEAT BY TRANSFORMATION MEDIATED BY AGROBACTERIUM AND THE COMBINATION OBTAINED BY THEM

Info

Publication number
EA199800212A1
EA199800212A1 EA199800212A EA199800212A EA199800212A1 EA 199800212 A1 EA199800212 A1 EA 199800212A1 EA 199800212 A EA199800212 A EA 199800212A EA 199800212 A EA199800212 A EA 199800212A EA 199800212 A1 EA199800212 A1 EA 199800212A1
Authority
EA
Eurasian Patent Office
Prior art keywords
transformation
methods
wheat
agrobacterium
productive
Prior art date
Application number
EA199800212A
Other languages
Russian (ru)
Inventor
Минг Ченг
Джойс Э. Фрай
Ючун Уон
Original Assignee
Монсанто Компани
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24677185&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA199800212(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Монсанто Компани filed Critical Монсанто Компани
Publication of EA199800212A1 publication Critical patent/EA199800212A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Grain Derivatives (AREA)
  • Soy Sauces And Products Related Thereto (AREA)
  • Seasonings (AREA)

Abstract

Раскрываются процессы получения устойчиво трансформируемой высокопродуктивной пшеницы и система трансформации пшеницы с помощью Agrobacterium. Настоящее изобретение представляет способы трансформации и различные эксплантаты, например свежевыделенные либо предварительно культивированные незрелые зародыши, зародышевый каллюс и суспензионные клетки. Раскрывают также способы выделения трансгенных растений после трансформации в течение непродолжительного периода времени при условии, если эксплантаты регенерируются во время трансформации. Таким образом, существенно снижается частота сомаклональных изменений, связанных с продолжительным периодом культивирования in vitro. Частота трансформации с использованием этой системы сопоставима либо превосходит опубликованные способы с использованием других систем, например бомбардировки микроносителями.Отчет о международном поиске опубликован 1998.03.19.The processes of obtaining stably transformed highly productive wheat and the system of wheat transformation using Agrobacterium are revealed. The present invention provides transformation methods and various explants, such as freshly isolated or pre-cultured immature embryos, germ callus and suspension cells. Methods for isolating transgenic plants after transformation for a short period of time are also disclosed, provided the explants are regenerated during transformation. Thus, the frequency of somaclonal changes associated with a long period of in vitro cultivation is significantly reduced. The frequency of transformation using this system is comparable or exceeds published methods using other systems, such as microcarrier bombardment. The international search report was published 1998.03.19.

EA199800212A 1996-06-21 1997-06-20 METHODS OF OBTAINING SUSTAINABLE TRANSFORMABLE HIGH-PRODUCTIVE WHEAT BY TRANSFORMATION MEDIATED BY AGROBACTERIUM AND THE COMBINATION OBTAINED BY THEM EA199800212A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66718896A 1996-06-21 1996-06-21
PCT/US1997/010621 WO1997048814A2 (en) 1996-06-21 1997-06-20 Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom

Publications (1)

Publication Number Publication Date
EA199800212A1 true EA199800212A1 (en) 1998-10-29

Family

ID=24677185

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199800212A EA199800212A1 (en) 1996-06-21 1997-06-20 METHODS OF OBTAINING SUSTAINABLE TRANSFORMABLE HIGH-PRODUCTIVE WHEAT BY TRANSFORMATION MEDIATED BY AGROBACTERIUM AND THE COMBINATION OBTAINED BY THEM

Country Status (11)

Country Link
US (1) US7026528B2 (en)
EP (1) EP0856060A2 (en)
CN (1) CN1208437A (en)
AU (1) AU738153C (en)
CA (1) CA2230216A1 (en)
CZ (1) CZ86798A3 (en)
EA (1) EA199800212A1 (en)
HU (1) HUP9902123A3 (en)
MX (1) MX9801421A (en)
TR (1) TR199800294T1 (en)
WO (1) WO1997048814A2 (en)

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CA2230216A1 (en) 1997-12-24
AU3402897A (en) 1998-01-07
MX9801421A (en) 1999-01-01
US7026528B2 (en) 2006-04-11
CN1208437A (en) 1999-02-17
HUP9902123A2 (en) 1999-10-28
US20030024014A1 (en) 2003-01-30
WO1997048814A3 (en) 1998-03-19
TR199800294T1 (en) 1999-10-21
EP0856060A2 (en) 1998-08-05
AU738153B2 (en) 2001-09-13
WO1997048814A2 (en) 1997-12-24
AU738153C (en) 2004-07-01
HUP9902123A3 (en) 2002-01-28
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