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DK4081652T3 - Styret strengforskydning for parret endesekvensering - Google Patents

Styret strengforskydning for parret endesekvensering Download PDF

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Publication number
DK4081652T3
DK4081652T3 DK20906868.3T DK20906868T DK4081652T3 DK 4081652 T3 DK4081652 T3 DK 4081652T3 DK 20906868 T DK20906868 T DK 20906868T DK 4081652 T3 DK4081652 T3 DK 4081652T3
Authority
DK
Denmark
Prior art keywords
sequenceing
paired end
controlled strand
strand shift
shift
Prior art date
Application number
DK20906868.3T
Other languages
English (en)
Inventor
Radoje Drmanac
Matthew J Callow
Original Assignee
Mgi Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mgi Tech Co Ltd filed Critical Mgi Tech Co Ltd
Application granted granted Critical
Publication of DK4081652T3 publication Critical patent/DK4081652T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK20906868.3T 2019-12-23 2020-12-23 Styret strengforskydning for parret endesekvensering DK4081652T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962952713P 2019-12-23 2019-12-23
PCT/CN2020/000311 WO2021128441A1 (en) 2019-12-23 2020-12-23 Controlled strand-displacement for paired-end sequencing

Publications (1)

Publication Number Publication Date
DK4081652T3 true DK4081652T3 (da) 2025-03-24

Family

ID=76438809

Family Applications (1)

Application Number Title Priority Date Filing Date
DK20906868.3T DK4081652T3 (da) 2019-12-23 2020-12-23 Styret strengforskydning for parret endesekvensering

Country Status (9)

Country Link
US (1) US12448648B2 (da)
EP (2) EP4524259A3 (da)
JP (1) JP2023507529A (da)
CN (3) CN120666005A (da)
AU (1) AU2020413071A1 (da)
CA (1) CA3163353A1 (da)
DK (1) DK4081652T3 (da)
ES (1) ES3025432T3 (da)
WO (1) WO2021128441A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12448648B2 (en) * 2019-12-23 2025-10-21 Mgi Tech Co., Ltd. Controlled strand-displacement for paired end sequencing
CN115516104A (zh) 2020-03-03 2022-12-23 加利福尼亚太平洋生物科学股份有限公司 用于对双链核酸进行测序的方法和组合物
US11427855B1 (en) * 2021-06-17 2022-08-30 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) * 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
EP4367264A1 (en) * 2021-07-08 2024-05-15 Pacific Biosciences Of California, Inc. Paired-end sequencing methods and compositions
CN120322565A (zh) * 2022-12-09 2025-07-15 深圳华大智造科技股份有限公司 Dnb双末端测序中二链的合成方法、测序方法及相关产品
WO2025062001A1 (en) * 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
WO2025212654A1 (en) * 2024-04-02 2025-10-09 Element Biosciences, Inc. Library molecule titration for tunable surface density in polony sequencing

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69837913T2 (de) 1997-04-01 2008-02-07 Solexa Ltd., Saffron Walden Verfahren zur vervielfältigung von nukleinsäure
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN102124018A (zh) * 2008-04-29 2011-07-13 生命科技公司 可维持从核苷酸模板酶促合成双链核苷酸的非天然聚合酶底物及其使用的方法
GB201018714D0 (en) 2010-11-05 2010-12-22 Oxitec Ltd Strand displacement activity of modified polymerases and uses thereof
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
EP2947156A1 (en) 2014-05-22 2015-11-25 Qiagen GmbH Optimization of sequencing reactions
GB201416106D0 (en) * 2014-09-11 2014-10-29 Illumina Cambridge Ltd Paired-end sequencing by attachment of the complementary strand
WO2016133764A1 (en) * 2015-02-17 2016-08-25 Complete Genomics, Inc. Dna sequencing using controlled strand displacement
EP3277839B1 (en) * 2015-03-31 2020-12-02 Illumina Cambridge Limited Surface concatamerization of templates
WO2016170182A1 (en) * 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
US20170283864A1 (en) * 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
HUE071446T2 (hu) 2017-01-04 2025-09-28 Mgi Tech Co Ltd Lépésenkénti szekvenálás nem jelölt reverzibilis terminátorokkal vagy természetes nukleotidokkal
WO2018208133A1 (ko) 2017-05-12 2018-11-15 서울대학교산학협력단 고순도 뉴클레오타이드 획득 방법 및 장치
CN110468179B (zh) * 2018-05-10 2021-03-05 北京大学 选择性扩增核酸序列的方法
US12448648B2 (en) * 2019-12-23 2025-10-21 Mgi Tech Co., Ltd. Controlled strand-displacement for paired end sequencing
CN115516104A (zh) * 2020-03-03 2022-12-23 加利福尼亚太平洋生物科学股份有限公司 用于对双链核酸进行测序的方法和组合物

Also Published As

Publication number Publication date
AU2020413071A1 (en) 2022-06-09
CN114846154A (zh) 2022-08-02
EP4081652A4 (en) 2023-12-06
CN114846154B (zh) 2025-06-17
JP2023507529A (ja) 2023-02-22
CN120666005A (zh) 2025-09-19
EP4524259A3 (en) 2025-05-14
EP4081652A1 (en) 2022-11-02
EP4081652B1 (en) 2025-03-19
EP4524259A2 (en) 2025-03-19
CN120666004A (zh) 2025-09-19
CA3163353A1 (en) 2021-07-01
ES3025432T3 (en) 2025-06-09
US12448648B2 (en) 2025-10-21
US20210189483A1 (en) 2021-06-24
WO2021128441A1 (en) 2021-07-01

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