DK3821011T3 - Transposom-aktiveret dna/rna-sekventering (ted rna-seq) - Google Patents
Transposom-aktiveret dna/rna-sekventering (ted rna-seq) Download PDFInfo
- Publication number
- DK3821011T3 DK3821011T3 DK19834769.2T DK19834769T DK3821011T3 DK 3821011 T3 DK3821011 T3 DK 3821011T3 DK 19834769 T DK19834769 T DK 19834769T DK 3821011 T3 DK3821011 T3 DK 3821011T3
- Authority
- DK
- Denmark
- Prior art keywords
- rna
- transposome
- ted
- seq
- activated dna
- Prior art date
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 title 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862696681P | 2018-07-11 | 2018-07-11 | |
| PCT/US2019/041121 WO2020014303A1 (en) | 2018-07-11 | 2019-07-10 | Transposome enabled dna/rna-sequencing (ted rna-seq) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3821011T3 true DK3821011T3 (da) | 2023-07-24 |
| DK3821011T5 DK3821011T5 (da) | 2024-08-19 |
Family
ID=69142494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK19834769.2T DK3821011T5 (da) | 2018-07-11 | 2019-07-10 | Transposom-aktiveret dna/rna-sekventering (ted rna-seq) |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20210222163A1 (da) |
| EP (1) | EP3821011B1 (da) |
| CN (1) | CN112689673A (da) |
| DK (1) | DK3821011T5 (da) |
| WO (1) | WO2020014303A1 (da) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| GB2513024B (en) | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| GB2546833B (en) | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| CN108728477B (zh) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | 一种高效的转座突变系统及构建方法 |
| AU2018281745B2 (en) | 2017-06-05 | 2022-05-19 | Becton, Dickinson And Company | Sample indexing for single cells |
| ES2945191T3 (es) | 2018-05-03 | 2023-06-29 | Becton Dickinson Co | Análisis de muestras multiómicas de alto rendimiento |
| EP4471156A3 (en) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| EP4025686A4 (en) | 2019-09-03 | 2023-09-13 | Myeloid Therapeutics, Inc. | Methods and compositions for genomic integration |
| CN112501249B (zh) * | 2019-09-16 | 2024-01-26 | 深圳市真迈生物科技有限公司 | Rna文库的制备方法、测序方法和试剂盒 |
| EP4407041A3 (en) | 2019-11-08 | 2024-09-25 | Becton Dickinson and Company | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| EP4471155A3 (en) | 2020-01-29 | 2024-12-18 | Becton, Dickinson and Company | Barcoded wells for spatial mapping of single cells through sequencing |
| CN111188094B (zh) * | 2020-02-24 | 2020-10-16 | 南京诺唯赞生物科技股份有限公司 | 一种用于病原微生物检测的测序文库构建方法和试剂盒 |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| CN112251422B (zh) * | 2020-10-21 | 2024-04-19 | 华中农业大学 | 含独特分子标签序列的转座酶复合体及其应用 |
| US20220135966A1 (en) | 2020-11-03 | 2022-05-05 | Fluent Biosciences Inc. | Systems and methods for making sequencing libraries |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| JP2024518100A (ja) | 2021-05-11 | 2024-04-24 | マイエロイド・セラピューティクス，インコーポレーテッド | ゲノム組込みのための方法および組成物 |
| EP4402280A1 (en) * | 2021-09-14 | 2024-07-24 | Becton, Dickinson and Company | Full length single cell rna sequencing |
| EP4605556A2 (en) * | 2022-10-20 | 2025-08-27 | Bio-Rad Laboratories, Inc. | Methods and compositions for tracking barcodes in partitions |
| WO2025024703A1 (en) * | 2023-07-26 | 2025-01-30 | Bio-Rad Laboratories, Inc. | Dual-tagmentation single-cell dnaseq |
| WO2025207588A2 (en) * | 2024-03-27 | 2025-10-02 | Bio-Rad Laboratories, Inc. | Single-cell sequencing using multiple transposase adapters |
| CN120665989B (zh) * | 2025-08-22 | 2025-12-12 | 北京大学 | 一种微量rna测序文库的构建方法及其所用的试剂 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| US20040197793A1 (en) * | 2002-08-30 | 2004-10-07 | Arjang Hassibi | Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| EP2664678B1 (en) * | 2008-10-24 | 2014-10-08 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| CA2827948C (en) * | 2011-02-23 | 2019-07-09 | Board Of Regents, University Of Texas System | Use of template switching for dna synthesis |
| AU2012304328B2 (en) * | 2011-09-09 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| US10557133B2 (en) * | 2013-03-13 | 2020-02-11 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| US10941397B2 (en) * | 2013-10-17 | 2021-03-09 | Takara Bio Usa, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
| US20170009288A1 (en) * | 2014-02-03 | 2017-01-12 | Thermo Fisher Scientific Baltics Uab | Method for controlled dna fragmentation |
| US20170044525A1 (en) * | 2014-04-29 | 2017-02-16 | Illumina, Inc. | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| CA2948951C (en) * | 2014-05-14 | 2023-04-04 | Ruprecht-Karls-Universitat Heidelberg | Synthesis of double-stranded nucleic acids |
| US10017759B2 (en) * | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| EP3286326B1 (en) * | 2015-04-23 | 2025-01-22 | Becton, Dickinson and Company | Method for whole transcriptome amplification |
| US10280449B2 (en) * | 2015-06-29 | 2019-05-07 | The Regents Of The University Of California | Methods of producing nucleic acid libraries and compositions and kits for practicing same |
| WO2017048993A1 (en) | 2015-09-15 | 2017-03-23 | Takara Bio Usa, Inc. | Methods for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and compositions for practicing the same |
| EP3485032B1 (en) * | 2016-07-12 | 2021-02-17 | Life Technologies Corporation | Compositions and methods for detecting nucleic acid regions |
| US10676736B2 (en) | 2016-08-05 | 2020-06-09 | Bio-Rad Laboratories, Inc. | Second strand direct |
-
2019
- 2019-07-10 DK DK19834769.2T patent/DK3821011T5/da active
- 2019-07-10 WO PCT/US2019/041121 patent/WO2020014303A1/en not_active Ceased
- 2019-07-10 US US17/257,991 patent/US20210222163A1/en not_active Abandoned
- 2019-07-10 CN CN201980059589.3A patent/CN112689673A/zh active Pending
- 2019-07-10 EP EP19834769.2A patent/EP3821011B1/en active Active
-
2024
- 2024-08-21 US US18/810,809 patent/US20240401033A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DK3821011T5 (da) | 2024-08-19 |
| US20210222163A1 (en) | 2021-07-22 |
| WO2020014303A1 (en) | 2020-01-16 |
| US20240401033A1 (en) | 2024-12-05 |
| CN112689673A (zh) | 2021-04-20 |
| EP3821011A4 (en) | 2022-03-30 |
| EP3821011B1 (en) | 2023-04-26 |
| EP3821011A1 (en) | 2021-05-19 |
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